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The map label for this gene is gacS [H]

Identifier: 222524521

GI number: 222524521

Start: 1583914

End: 1586154

Strand: Direct

Name: gacS [H]

Synonym: Chy400_1245

Alternate gene names: 222524521

Gene position: 1583914-1586154 (Clockwise)

Preceding gene: 222524520

Following gene: 222524526

Centisome position: 30.06

GC content: 52.16

Gene sequence:

>2241_bases
ATGTGGATGATCGGGAGCAGTGCCGCAATTTTCATCGGCGGTGGCTGGTTTATTAACCGCTACTTTACTCAGCCACTCAT
CACATTGACAAACGCAGCGCGACGAGTTGCCGAGGGATGGCGCGATACACCAATCCCGCTCGTCGGTACTGATGAAATTG
GTGACCTTGCCCGCTCATTAGCGAGGCTAACTGCCAGCTTGAATGCAACGATTGCTACACTTGAAGAGCGCATCCAACAG
CGTACTGCACAATTGCAAGACGCCTTACGCGAAAAAGAGACGGTTCTTGTCCGTTCGCAGCAGCAACGATGGCGCGAACG
GACATTGATTGATTTGAGTTTGCGCTTGAATAGTGCCCACAGCGAGGAGGAGATTTATCGATCTATCGTCGATGCGTTTG
TTGCCATTCGTGAAAATCAAGATCGGATTGGTATCTACGTCCGTGATACGGATTCAAGGCAATGGCTGCCCTACATTACC
TATGGTTATCAGCATAATCCCCTCCTTACGCTGACGGCGCCGCCTGCACTTGAGCAGTATACAGCTAATCCGTTCTACAT
TCCTGATCTTCGGCAGCATCAGTTCACAGACCTGATCCAGGCCGAGGGATCGGCGATTGTGGCAACGATTGCCGTGCAAG
GACATCCCCAGGCCATACTGGTTGTCTATCGTCCACAGCCTCACGCCTTCGATCACGCTGATATTGAGAACTTGCGCCTT
GCTGCGCAGTTAACGACGTATGCGCTTAATCGGGTATCCATAATGGCTGAAATGGCTGAATTACAGCGTGCCCGAGAACG
CGCTGAGGTGGCCGTCCGGGAACGCAGCACATTTTTGGTTCGTCTCGATCACGATATTCGTCAACCGTTAAATACCATTA
TTGCGCTCAGTGAATTGTTGCGCGAGGCGCTGGCCACCCAGACCATGTTTGCCGAAGATATCGACAAGATTCGGCGTGCC
GGACGTCACCTGGTGAACCGTTTTAACCTTCTTCTCGACAGTGCAAAAATCGAGATCGGTTCTCTCAGCTTACAACCGGA
ACCTTTTATCTTTGATGCCCTGCTCGATAATATTCTTGAAGAAATATCACCTCTCATCCACCAACACCAGCATCGTTTTG
ACATTGAGCGCCCTGCACAAGTGGGGATGATCGTAGCTGATCTGAATCGGTTACGTCAGATCTTTATCTATCCGCTGCGT
TTTGCTGCCACGACCACGATGCGCGGTGTGATCACACTACGTGTACAGCGATTGAGTGGCGACGAAATAGACACACTCGA
AGTCATAATCACCGATACTGGACCATCACTGAGTTCAGAGCATCTGAATGCACTACTTACGCCTTTCGCCGTTCCCCCAG
ACACAACACGCCAGTTAGATGAAGGATGTGGTCTGGCGCTGAGCCGTCAATTGTGTGAGTTGATGGGAGGGACATTTCAG
GCAGTTCCTCGTACTGCGGGTGGAGTCAGGTTCACTATTCGTATCCCTCTTAGCACGCTACGCAATGCTGAGGAAGTGTC
ACCGCCGGCGTACCTCCCTCAGCCTGATCTGGTGTTGATTACGACAACGCAGGCACGTGTGCTACAGTCAGCATTAGAAC
AATTTGGCTGGCAGGTGCAAAGCGAAGCCAGTCTTGCGCATGTGCTAAATCGTCTCTACCGTCCGCCTGCTGCCATTCTG
ATGGATGTACCGGAAAATCGTGATCTGGTGGTAGCGACCTTACAGGCTGCCGGCTGGCAAGATGTGCCGATCATCTGGTT
GACCGATAACGTTGAGAGTACCGGTAGCACCGATTATGCAGTATGGCCGGGGGAGACTGAACAGGTGATTCAGACGGTGC
AGGTCGTGTTATCTCGCCGCTCAAGAACTGCAATGTCGCGCACCATTCTGGTAATTGATGATGAACCTCCAACACGGCTG
ATGATCCGTCGGGCATTGGAGAGTGATGGTTGGGTTGTATTGGAAGCAAACAACGGGCAGATTGGAGCAACCATCTGGCG
TACCAATAAGCCACAACTGGTGATCATGGATATGACGTTGCCGGACGTTGATGGTCTGGTACTCTTACGCGAAGTGCGCG
CCGATCTGGAAACACCGGTGATTGTGGTATCAGAGCGGACACTGCGGCGCGAGGAGCTGGAACTTCTTGCCAGTATTGGC
GCAGTCGTGTTGCAGAAGGGGCGTTATCGACGGGGTGATCTCCTTGAGCTGGTTCGCAAACTGATATGGGAACGGCTGTG
A

Upstream 100 bases:

>100_bases
CCTGCTGATCATCAGCGAACTTACGCGCATACTGAAATCTGAACAACAACAAGTAAATGCCTCTTTTTCGACAACGATCT
GGATTGGCTTCTGGCTCGTG

Downstream 100 bases:

>100_bases
ACCGCATTCTTACTGCACCGGCGGGAGCACTGATGCAGCGTCAGGGTGATGGTTGGGTAGCGTGTCATGCATTCGCTGCT
ACCAGATGTGAAATCATACA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MWMIGSSAAIFIGGGWFINRYFTQPLITLTNAARRVAEGWRDTPIPLVGTDEIGDLARSLARLTASLNATIATLEERIQQ
RTAQLQDALREKETVLVRSQQQRWRERTLIDLSLRLNSAHSEEEIYRSIVDAFVAIRENQDRIGIYVRDTDSRQWLPYIT
YGYQHNPLLTLTAPPALEQYTANPFYIPDLRQHQFTDLIQAEGSAIVATIAVQGHPQAILVVYRPQPHAFDHADIENLRL
AAQLTTYALNRVSIMAEMAELQRARERAEVAVRERSTFLVRLDHDIRQPLNTIIALSELLREALATQTMFAEDIDKIRRA
GRHLVNRFNLLLDSAKIEIGSLSLQPEPFIFDALLDNILEEISPLIHQHQHRFDIERPAQVGMIVADLNRLRQIFIYPLR
FAATTTMRGVITLRVQRLSGDEIDTLEVIITDTGPSLSSEHLNALLTPFAVPPDTTRQLDEGCGLALSRQLCELMGGTFQ
AVPRTAGGVRFTIRIPLSTLRNAEEVSPPAYLPQPDLVLITTTQARVLQSALEQFGWQVQSEASLAHVLNRLYRPPAAIL
MDVPENRDLVVATLQAAGWQDVPIIWLTDNVESTGSTDYAVWPGETEQVIQTVQVVLSRRSRTAMSRTILVIDDEPPTRL
MIRRALESDGWVVLEANNGQIGATIWRTNKPQLVIMDMTLPDVDGLVLLREVRADLETPVIVVSERTLRREELELLASIG
AVVLQKGRYRRGDLLELVRKLIWERL

Sequences:

>Translated_746_residues
MWMIGSSAAIFIGGGWFINRYFTQPLITLTNAARRVAEGWRDTPIPLVGTDEIGDLARSLARLTASLNATIATLEERIQQ
RTAQLQDALREKETVLVRSQQQRWRERTLIDLSLRLNSAHSEEEIYRSIVDAFVAIRENQDRIGIYVRDTDSRQWLPYIT
YGYQHNPLLTLTAPPALEQYTANPFYIPDLRQHQFTDLIQAEGSAIVATIAVQGHPQAILVVYRPQPHAFDHADIENLRL
AAQLTTYALNRVSIMAEMAELQRARERAEVAVRERSTFLVRLDHDIRQPLNTIIALSELLREALATQTMFAEDIDKIRRA
GRHLVNRFNLLLDSAKIEIGSLSLQPEPFIFDALLDNILEEISPLIHQHQHRFDIERPAQVGMIVADLNRLRQIFIYPLR
FAATTTMRGVITLRVQRLSGDEIDTLEVIITDTGPSLSSEHLNALLTPFAVPPDTTRQLDEGCGLALSRQLCELMGGTFQ
AVPRTAGGVRFTIRIPLSTLRNAEEVSPPAYLPQPDLVLITTTQARVLQSALEQFGWQVQSEASLAHVLNRLYRPPAAIL
MDVPENRDLVVATLQAAGWQDVPIIWLTDNVESTGSTDYAVWPGETEQVIQTVQVVLSRRSRTAMSRTILVIDDEPPTRL
MIRRALESDGWVVLEANNGQIGATIWRTNKPQLVIMDMTLPDVDGLVLLREVRADLETPVIVVSERTLRREELELLASIG
AVVLQKGRYRRGDLLELVRKLIWERL
>Mature_746_residues
MWMIGSSAAIFIGGGWFINRYFTQPLITLTNAARRVAEGWRDTPIPLVGTDEIGDLARSLARLTASLNATIATLEERIQQ
RTAQLQDALREKETVLVRSQQQRWRERTLIDLSLRLNSAHSEEEIYRSIVDAFVAIRENQDRIGIYVRDTDSRQWLPYIT
YGYQHNPLLTLTAPPALEQYTANPFYIPDLRQHQFTDLIQAEGSAIVATIAVQGHPQAILVVYRPQPHAFDHADIENLRL
AAQLTTYALNRVSIMAEMAELQRARERAEVAVRERSTFLVRLDHDIRQPLNTIIALSELLREALATQTMFAEDIDKIRRA
GRHLVNRFNLLLDSAKIEIGSLSLQPEPFIFDALLDNILEEISPLIHQHQHRFDIERPAQVGMIVADLNRLRQIFIYPLR
FAATTTMRGVITLRVQRLSGDEIDTLEVIITDTGPSLSSEHLNALLTPFAVPPDTTRQLDEGCGLALSRQLCELMGGTFQ
AVPRTAGGVRFTIRIPLSTLRNAEEVSPPAYLPQPDLVLITTTQARVLQSALEQFGWQVQSEASLAHVLNRLYRPPAAIL
MDVPENRDLVVATLQAAGWQDVPIIWLTDNVESTGSTDYAVWPGETEQVIQTVQVVLSRRSRTAMSRTILVIDDEPPTRL
MIRRALESDGWVVLEANNGQIGATIWRTNKPQLVIMDMTLPDVDGLVLLREVRADLETPVIVVSERTLRREELELLASIG
AVVLQKGRYRRGDLLELVRKLIWERL

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=337, Percent_Identity=29.080118694362, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789149, Length=502, Percent_Identity=22.9083665338645, Blast_Score=90, Evalue=7e-19,
Organism=Escherichia coli, GI87081816, Length=272, Percent_Identity=29.7794117647059, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786912, Length=267, Percent_Identity=24.7191011235955, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI145693157, Length=276, Percent_Identity=27.1739130434783, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=255, Percent_Identity=23.5294117647059, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1786911, Length=94, Percent_Identity=40.4255319148936, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 84258; Mature: 84258

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50885 HAMP ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWMIGSSAAIFIGGGWFINRYFTQPLITLTNAARRVAEGWRDTPIPLVGTDEIGDLARSL
CEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHH
ARLTASLNATIATLEERIQQRTAQLQDALREKETVLVRSQQQRWRERTLIDLSLRLNSAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
SEEEIYRSIVDAFVAIRENQDRIGIYVRDTDSRQWLPYITYGYQHNPLLTLTAPPALEQY
CHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCEEEEECCCCHHHC
TANPFYIPDLRQHQFTDLIQAEGSAIVATIAVQGHPQAILVVYRPQPHAFDHADIENLRL
CCCCEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHH
AAQLTTYALNRVSIMAEMAELQRARERAEVAVRERSTFLVRLDHDIRQPLNTIIALSELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHH
REALATQTMFAEDIDKIRRAGRHLVNRFNLLLDSAKIEIGSLSLQPEPFIFDALLDNILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHH
EISPLIHQHQHRFDIERPAQVGMIVADLNRLRQIFIYPLRFAATTTMRGVITLRVQRLSG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCC
DEIDTLEVIITDTGPSLSSEHLNALLTPFAVPPDTTRQLDEGCGLALSRQLCELMGGTFQ
CCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCEE
AVPRTAGGVRFTIRIPLSTLRNAEEVSPPAYLPQPDLVLITTTQARVLQSALEQFGWQVQ
ECCCCCCCEEEEEEECHHHCCCHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCEEC
SEASLAHVLNRLYRPPAAILMDVPENRDLVVATLQAAGWQDVPIIWLTDNVESTGSTDYA
CCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCEE
VWPGETEQVIQTVQVVLSRRSRTAMSRTILVIDDEPPTRLMIRRALESDGWVVLEANNGQ
ECCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCE
IGATIWRTNKPQLVIMDMTLPDVDGLVLLREVRADLETPVIVVSERTLRREELELLASIG
EEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHH
AVVLQKGRYRRGDLLELVRKLIWERL
HHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MWMIGSSAAIFIGGGWFINRYFTQPLITLTNAARRVAEGWRDTPIPLVGTDEIGDLARSL
CEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHH
ARLTASLNATIATLEERIQQRTAQLQDALREKETVLVRSQQQRWRERTLIDLSLRLNSAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
SEEEIYRSIVDAFVAIRENQDRIGIYVRDTDSRQWLPYITYGYQHNPLLTLTAPPALEQY
CHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCEEEEECCCCHHHC
TANPFYIPDLRQHQFTDLIQAEGSAIVATIAVQGHPQAILVVYRPQPHAFDHADIENLRL
CCCCEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHH
AAQLTTYALNRVSIMAEMAELQRARERAEVAVRERSTFLVRLDHDIRQPLNTIIALSELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHH
REALATQTMFAEDIDKIRRAGRHLVNRFNLLLDSAKIEIGSLSLQPEPFIFDALLDNILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHH
EISPLIHQHQHRFDIERPAQVGMIVADLNRLRQIFIYPLRFAATTTMRGVITLRVQRLSG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCC
DEIDTLEVIITDTGPSLSSEHLNALLTPFAVPPDTTRQLDEGCGLALSRQLCELMGGTFQ
CCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCEE
AVPRTAGGVRFTIRIPLSTLRNAEEVSPPAYLPQPDLVLITTTQARVLQSALEQFGWQVQ
ECCCCCCCEEEEEEECHHHCCCHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCEEC
SEASLAHVLNRLYRPPAAILMDVPENRDLVVATLQAAGWQDVPIIWLTDNVESTGSTDYA
CCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCEE
VWPGETEQVIQTVQVVLSRRSRTAMSRTILVIDDEPPTRLMIRRALESDGWVVLEANNGQ
ECCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCE
IGATIWRTNKPQLVIMDMTLPDVDGLVLLREVRADLETPVIVVSERTLRREELELLASIG
EEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHH
AVVLQKGRYRRGDLLELVRKLIWERL
HHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]