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The map label for this gene is recJ [H]

Identifier: 222524518

GI number: 222524518

Start: 1580122

End: 1581834

Strand: Reverse

Name: recJ [H]

Synonym: Chy400_1242

Alternate gene names: 222524518

Gene position: 1581834-1580122 (Counterclockwise)

Preceding gene: 222524522

Following gene: 222524515

Centisome position: 30.02

GC content: 57.09

Gene sequence:

>1713_bases
ATGTCAGCTCGCCGTCAGCGCTGGGAGATCCGTCCGCCAGCACCCACTTCTTTCGTTGAGCAACTTGGTCTGCACCCGTT
GCTTGCACACCTTCTTTATCAGCGTGGCTTGCATGAGCCATCCATCGCCAGAGCTTTTCTTAACGCTGATTACAGCTCCG
GCCTGCACGATCCGTTTGCCATGCGAGGTATGGCAGCCGCTGCCGGTCGTATTGCCGAAGCGATTGAGCGGGGCGAATTG
ATCGCCGTCTACGGCGATTTTGATGTCGATGGGATCACTGCGGTTGCCCTGCTGACGCAGGCGATCAAGGCGATGGGGGG
ACGGATTCGGCCATACATTCCGCATCGTGCCCGCGAAGGGTATGGTCTTAACAATCTGGCGATTAGTCAGTTGGTGGCCG
ATGGTGTCACATTGCTGATAACGGTCGACTGCGGCATTTCCAATTATGCAGAGGTAGCCGAAGCCAATCGGCTCGGCCTC
GATGTGATTGTCACTGATCACCACCATCCACCCATGTTGCTGCCGCCAGCCCTGGCAATAGTGAATCCACGCCAGCCCGA
CTGTGCCTATCCATTCAAAGGTCTGGTTGGGGTCGGGATTGCCTTCAAAGTCGTGCAGGCACTGGCACGCTACGGTAAGC
GGCCAGCCGGTTTACGTGGACGTGATCTGCTCGATCTGGTTGCGCTAGGCACTGTCGCCGATATGGGTCCACTCCACGAC
GAGAATCGGGTGTTGGTACGGGCCGGCTTGATTGCGCTCAATGAAAGTTCGCGTCCTGGTGTCCTGGCATTGGTGGCGGC
TGCCGGCCTGTCGCCGGGTATGATCGACAGCGGCGCAATTGCGTTCGCTCTGGCACCGCGCCTCAACGCAGCCGGTCGCT
TAGCCGATGCGCGTCGTGCGTATGAGCTGCTCCTGGCTGATGAGCGAATGAAAGCCGATCTGCTGGCTGCCGAATTGAAT
ACCACCAATCGTGAGCGGCAGGCATTGACCCGGCAATTATATGCCATGGCAGAAACCATGGTGATGCAGAGTGGTCGCAC
CGAACATCCGCTGATTGTCGTCTCGCACCCCGGTTTTAACGCGGGTGTTATCGGATTGGTCGCCGCCCGTTTGGTTGAGA
CCTATCATCGTCCGGTAATTGTGATCGAGCAAGGCGAGGAGACATCACGTGGATCAGCGCGCTCGATCCCCGGGTTTAGT
ATCATCGATCTGTTCGATCAATGTGCCGATCTCTTTGTCCGTTATGGTGGACATGCTGCGGCTGCCGGTTTTACGATTTC
CACGAACCAGATTACGGCGCTCGAACAGCGGTTACTGGCCCTGGGGAGCCAGCATCTGTCAGCCGAACAGCTCGTACCAC
GATTGTTGATCGATGCCACTGTGCCATTAAGTGATTTATCGTGGGATCTATACGCTACCCTGTGCCAGCTCGAACCGTTT
GGACAGGGTAATCCGCAGCCTGTGTTCATGACCCCCAATGTTACAGTCATCGATCCGCAACCGACAACTGATGGCAATCA
TCTGCGGATGCGTGTGCGTGTCGGTCAGACCTCGTTCGAGGCTATCGGTTTTCACTTTGGTCGCTTCGCGGATGCGCTTC
GTCGTCATCCAAACATCGATCTGGCGTACCAGTTGGCAGTTGATGAATGGAACGGACAGCGTCGCTTACGGCTGCTGGTG
CGCGATTTCCGGCGCGCAACGAAGAGCGAGTGA

Upstream 100 bases:

>100_bases
ATGGAGATACGTATGAAAAATCGTTGCAATTACCTCTGAGATGAAAACAGCGTATAATAATCGCCGCTTGCCATGATGCT
ATTGGCCAGGAGTTACAGGT

Downstream 100 bases:

>100_bases
GTGGAGAAGATGGCAGCAATCCTGGCATCGTGCAGATTGTTATGCCCGTGCCGGCACGCTGTCAGATGTGCTACCAACTA
AATTGCTGCCATCGGTGCAC

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MSARRQRWEIRPPAPTSFVEQLGLHPLLAHLLYQRGLHEPSIARAFLNADYSSGLHDPFAMRGMAAAAGRIAEAIERGEL
IAVYGDFDVDGITAVALLTQAIKAMGGRIRPYIPHRAREGYGLNNLAISQLVADGVTLLITVDCGISNYAEVAEANRLGL
DVIVTDHHHPPMLLPPALAIVNPRQPDCAYPFKGLVGVGIAFKVVQALARYGKRPAGLRGRDLLDLVALGTVADMGPLHD
ENRVLVRAGLIALNESSRPGVLALVAAAGLSPGMIDSGAIAFALAPRLNAAGRLADARRAYELLLADERMKADLLAAELN
TTNRERQALTRQLYAMAETMVMQSGRTEHPLIVVSHPGFNAGVIGLVAARLVETYHRPVIVIEQGEETSRGSARSIPGFS
IIDLFDQCADLFVRYGGHAAAAGFTISTNQITALEQRLLALGSQHLSAEQLVPRLLIDATVPLSDLSWDLYATLCQLEPF
GQGNPQPVFMTPNVTVIDPQPTTDGNHLRMRVRVGQTSFEAIGFHFGRFADALRRHPNIDLAYQLAVDEWNGQRRLRLLV
RDFRRATKSE

Sequences:

>Translated_570_residues
MSARRQRWEIRPPAPTSFVEQLGLHPLLAHLLYQRGLHEPSIARAFLNADYSSGLHDPFAMRGMAAAAGRIAEAIERGEL
IAVYGDFDVDGITAVALLTQAIKAMGGRIRPYIPHRAREGYGLNNLAISQLVADGVTLLITVDCGISNYAEVAEANRLGL
DVIVTDHHHPPMLLPPALAIVNPRQPDCAYPFKGLVGVGIAFKVVQALARYGKRPAGLRGRDLLDLVALGTVADMGPLHD
ENRVLVRAGLIALNESSRPGVLALVAAAGLSPGMIDSGAIAFALAPRLNAAGRLADARRAYELLLADERMKADLLAAELN
TTNRERQALTRQLYAMAETMVMQSGRTEHPLIVVSHPGFNAGVIGLVAARLVETYHRPVIVIEQGEETSRGSARSIPGFS
IIDLFDQCADLFVRYGGHAAAAGFTISTNQITALEQRLLALGSQHLSAEQLVPRLLIDATVPLSDLSWDLYATLCQLEPF
GQGNPQPVFMTPNVTVIDPQPTTDGNHLRMRVRVGQTSFEAIGFHFGRFADALRRHPNIDLAYQLAVDEWNGQRRLRLLV
RDFRRATKSE
>Mature_569_residues
SARRQRWEIRPPAPTSFVEQLGLHPLLAHLLYQRGLHEPSIARAFLNADYSSGLHDPFAMRGMAAAAGRIAEAIERGELI
AVYGDFDVDGITAVALLTQAIKAMGGRIRPYIPHRAREGYGLNNLAISQLVADGVTLLITVDCGISNYAEVAEANRLGLD
VIVTDHHHPPMLLPPALAIVNPRQPDCAYPFKGLVGVGIAFKVVQALARYGKRPAGLRGRDLLDLVALGTVADMGPLHDE
NRVLVRAGLIALNESSRPGVLALVAAAGLSPGMIDSGAIAFALAPRLNAAGRLADARRAYELLLADERMKADLLAAELNT
TNRERQALTRQLYAMAETMVMQSGRTEHPLIVVSHPGFNAGVIGLVAARLVETYHRPVIVIEQGEETSRGSARSIPGFSI
IDLFDQCADLFVRYGGHAAAAGFTISTNQITALEQRLLALGSQHLSAEQLVPRLLIDATVPLSDLSWDLYATLCQLEPFG
QGNPQPVFMTPNVTVIDPQPTTDGNHLRMRVRVGQTSFEAIGFHFGRFADALRRHPNIDLAYQLAVDEWNGQRRLRLLVR
DFRRATKSE

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=523, Percent_Identity=33.8432122370937, Blast_Score=262, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 61982; Mature: 61851

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARRQRWEIRPPAPTSFVEQLGLHPLLAHLLYQRGLHEPSIARAFLNADYSSGLHDPFA
CCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHH
MRGMAAAAGRIAEAIERGELIAVYGDFDVDGITAVALLTQAIKAMGGRIRPYIPHRAREG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
YGLNNLAISQLVADGVTLLITVDCGISNYAEVAEANRLGLDVIVTDHHHPPMLLPPALAI
CCCCHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEE
VNPRQPDCAYPFKGLVGVGIAFKVVQALARYGKRPAGLRGRDLLDLVALGTVADMGPLHD
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
ENRVLVRAGLIALNESSRPGVLALVAAAGLSPGMIDSGAIAFALAPRLNAAGRLADARRA
CCEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
YELLLADERMKADLLAAELNTTNRERQALTRQLYAMAETMVMQSGRTEHPLIVVSHPGFN
HHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
AGVIGLVAARLVETYHRPVIVIEQGEETSRGSARSIPGFSIIDLFDQCADLFVRYGGHAA
CHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
AAGFTISTNQITALEQRLLALGSQHLSAEQLVPRLLIDATVPLSDLSWDLYATLCQLEPF
CCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCC
GQGNPQPVFMTPNVTVIDPQPTTDGNHLRMRVRVGQTSFEAIGFHFGRFADALRRHPNID
CCCCCCCEEECCCEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
LAYQLAVDEWNGQRRLRLLVRDFRRATKSE
EEEEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SARRQRWEIRPPAPTSFVEQLGLHPLLAHLLYQRGLHEPSIARAFLNADYSSGLHDPFA
CCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHH
MRGMAAAAGRIAEAIERGELIAVYGDFDVDGITAVALLTQAIKAMGGRIRPYIPHRAREG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
YGLNNLAISQLVADGVTLLITVDCGISNYAEVAEANRLGLDVIVTDHHHPPMLLPPALAI
CCCCHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEE
VNPRQPDCAYPFKGLVGVGIAFKVVQALARYGKRPAGLRGRDLLDLVALGTVADMGPLHD
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
ENRVLVRAGLIALNESSRPGVLALVAAAGLSPGMIDSGAIAFALAPRLNAAGRLADARRA
CCEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
YELLLADERMKADLLAAELNTTNRERQALTRQLYAMAETMVMQSGRTEHPLIVVSHPGFN
HHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
AGVIGLVAARLVETYHRPVIVIEQGEETSRGSARSIPGFSIIDLFDQCADLFVRYGGHAA
CHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
AAGFTISTNQITALEQRLLALGSQHLSAEQLVPRLLIDATVPLSDLSWDLYATLCQLEPF
CCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCC
GQGNPQPVFMTPNVTVIDPQPTTDGNHLRMRVRVGQTSFEAIGFHFGRFADALRRHPNID
CCCCCCCEEECCCEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
LAYQLAVDEWNGQRRLRLLVRDFRRATKSE
EEEEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]