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The map label for this gene is gca [H]

Identifier: 222524501

GI number: 222524501

Start: 1560390

End: 1561364

Strand: Reverse

Name: gca [H]

Synonym: Chy400_1225

Alternate gene names: 222524501

Gene position: 1561364-1560390 (Counterclockwise)

Preceding gene: 222524503

Following gene: 222524500

Centisome position: 29.63

GC content: 54.36

Gene sequence:

>975_bases
ATGGCTCCAAAGCGTGCGTTGATCACCGGTATTACTGGTCAAGATGGCAGTTACCTTGCCGAGTTCCTCCTTGCCAGGGG
GTATGAGGTGATCGGCATGGTGCGTCGCTCGAGCACCGTCAATTTCGAGCGTATCAGGCATATTCAGGACAAAATCACGC
TGGTAAGTGGCGACCTGCTCGATGAAGTGTCGCTGATCCATATCCTGCGCGAGCATCGCCCACACGAGGTGTACAATCTG
GCTGCGCAGTCGTTTGTGCAGACATCGTGGCCCCAACCTGTGTTCACCGGCGAAACGACCGCTCTGGGTGTGACCCGCTT
ACTTGATGCGGTACGGATTGTTGACCCCGAAATCCGCTTTTATCAGGCGTCGAGCAGTGAGATGTTCGGCAAAGTGGTGG
AGGTGCCCCAGAAGGAGACGACACCTTTTTATCCGCGAAGCCCCTACGGGGTGGCTAAGGTCTATGGTCACTGGATCACG
GTCAACTATCGTGAGAGCTACGGCATGCACGCTACGTCGGGCATTCTGTTCAATCACGAGAGTCCGCGTCGTGGTCTCGA
ATTTGTGACGCGCAAGATTTCTCATGGCGTGGCGCGAATTAAACTCGGTCTCGATGAAGAACTACGCCTGGGTAATCTTG
AAGCGCGGCGGGATTGGGGGTTTGCCGGCGATTACGTGGAGGCAATGTGGCTGATGTTGCAGCAGGATCAGCCAGAAGAT
TACGTGATCGCTACCGGTGAGACCCATTCGGTTCGTGAGTTTTGTGAACTGGCATTTGGTTATGTTGGTCTTGACTATCG
CGATTATGTGGTGATCGATCAGCGCTTTATGCGTCCGGCTGAAGTCGATTTGCTGATCGGTGACGCCAGCAAGGCTCGCC
AAAAACTGGGCTGGCAACCACGCACTACGTTCCCTGAACTGGTGCAGATGATGGTTGAGGCTGATCTACAGTTAGTTAAG
GAGCAGATGCGATGA

Upstream 100 bases:

>100_bases
ACACACAACTCGTTTCATTGTATAGTAGCACCGTTTGTCGCAGTGCGAGAACGTTTGCTCGTGCTGGTTTTGTGCTGCTA
ACGTGAGAGGATAACAAAGT

Downstream 100 bases:

>100_bases
ATCAAGCTGAGACTCGGTTGATCGAGGAAGGATGGGCAGCATTGGTTGAGCGTCTGGGTCTGGCGAATGCAAGTCGGTTT
ATTATGTTGCTGGATCGACG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAPKRALITGITGQDGSYLAEFLLARGYEVIGMVRRSSTVNFERIRHIQDKITLVSGDLLDEVSLIHILREHRPHEVYNL
AAQSFVQTSWPQPVFTGETTALGVTRLLDAVRIVDPEIRFYQASSSEMFGKVVEVPQKETTPFYPRSPYGVAKVYGHWIT
VNYRESYGMHATSGILFNHESPRRGLEFVTRKISHGVARIKLGLDEELRLGNLEARRDWGFAGDYVEAMWLMLQQDQPED
YVIATGETHSVREFCELAFGYVGLDYRDYVVIDQRFMRPAEVDLLIGDASKARQKLGWQPRTTFPELVQMMVEADLQLVK
EQMR

Sequences:

>Translated_324_residues
MAPKRALITGITGQDGSYLAEFLLARGYEVIGMVRRSSTVNFERIRHIQDKITLVSGDLLDEVSLIHILREHRPHEVYNL
AAQSFVQTSWPQPVFTGETTALGVTRLLDAVRIVDPEIRFYQASSSEMFGKVVEVPQKETTPFYPRSPYGVAKVYGHWIT
VNYRESYGMHATSGILFNHESPRRGLEFVTRKISHGVARIKLGLDEELRLGNLEARRDWGFAGDYVEAMWLMLQQDQPED
YVIATGETHSVREFCELAFGYVGLDYRDYVVIDQRFMRPAEVDLLIGDASKARQKLGWQPRTTFPELVQMMVEADLQLVK
EQMR
>Mature_323_residues
APKRALITGITGQDGSYLAEFLLARGYEVIGMVRRSSTVNFERIRHIQDKITLVSGDLLDEVSLIHILREHRPHEVYNLA
AQSFVQTSWPQPVFTGETTALGVTRLLDAVRIVDPEIRFYQASSSEMFGKVVEVPQKETTPFYPRSPYGVAKVYGHWITV
NYRESYGMHATSGILFNHESPRRGLEFVTRKISHGVARIKLGLDEELRLGNLEARRDWGFAGDYVEAMWLMLQQDQPEDY
VIATGETHSVREFCELAFGYVGLDYRDYVVIDQRFMRPAEVDLLIGDASKARQKLGWQPRTTFPELVQMMVEADLQLVKE
QMR

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=55.2631578947368, Blast_Score=378, Evalue=1e-105,
Organism=Escherichia coli, GI1788366, Length=355, Percent_Identity=52.9577464788732, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI48994969, Length=335, Percent_Identity=24.7761194029851, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=22.9461756373938, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=348, Percent_Identity=52.0114942528736, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901790, Length=348, Percent_Identity=52.0114942528736, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17539422, Length=348, Percent_Identity=52.0114942528736, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901788, Length=348, Percent_Identity=52.0114942528736, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901786, Length=348, Percent_Identity=52.2988505747126, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=52.6162790697674, Blast_Score=358, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24158427, Length=346, Percent_Identity=53.757225433526, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21356223, Length=306, Percent_Identity=24.8366013071895, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 37092; Mature: 36961

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPKRALITGITGQDGSYLAEFLLARGYEVIGMVRRSSTVNFERIRHIQDKITLVSGDLL
CCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHEEEECHHH
DEVSLIHILREHRPHEVYNLAAQSFVQTSWPQPVFTGETTALGVTRLLDAVRIVDPEIRF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
YQASSSEMFGKVVEVPQKETTPFYPRSPYGVAKVYGHWITVNYRESYGMHATSGILFNHE
EECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEEEEEEEEHHCCCCCCCCCEEECCC
SPRRGLEFVTRKISHGVARIKLGLDEELRLGNLEARRDWGFAGDYVEAMWLMLQQDQPED
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCC
YVIATGETHSVREFCELAFGYVGLDYRDYVVIDQRFMRPAEVDLLIGDASKARQKLGWQP
EEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCCCEEEEECCHHHHHHHCCCCC
RTTFPELVQMMVEADLQLVKEQMR
CCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APKRALITGITGQDGSYLAEFLLARGYEVIGMVRRSSTVNFERIRHIQDKITLVSGDLL
CCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHEEEECHHH
DEVSLIHILREHRPHEVYNLAAQSFVQTSWPQPVFTGETTALGVTRLLDAVRIVDPEIRF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
YQASSSEMFGKVVEVPQKETTPFYPRSPYGVAKVYGHWITVNYRESYGMHATSGILFNHE
EECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEEEEEEEEHHCCCCCCCCCEEECCC
SPRRGLEFVTRKISHGVARIKLGLDEELRLGNLEARRDWGFAGDYVEAMWLMLQQDQPED
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCC
YVIATGETHSVREFCELAFGYVGLDYRDYVVIDQRFMRPAEVDLLIGDASKARQKLGWQP
EEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCCCEEEEECCHHHHHHHCCCCC
RTTFPELVQMMVEADLQLVKEQMR
CCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]