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The map label for this gene is spsK [H]

Identifier: 222524495

GI number: 222524495

Start: 1554632

End: 1555489

Strand: Reverse

Name: spsK [H]

Synonym: Chy400_1219

Alternate gene names: 222524495

Gene position: 1555489-1554632 (Counterclockwise)

Preceding gene: 222524496

Following gene: 222524481

Centisome position: 29.52

GC content: 60.72

Gene sequence:

>858_bases
ATGAATGGCGAACGGTATCTGATCACCGGTGGTACAGGGTACCTGGGGCAGGCGTTGATCCGTCACGCCCGTTGTCTGGG
TGTGCAGATTGCTGCTACCTTTCACTCGCAGACTCCGCCAGCGCTGGCAGAGGTTGCCTGGCATCCCCTCGATCTCCGCG
ACGCAGCGGCGGTTATGGCATTGATCCGCGAGGTGCAGCCGACCGTGATTGTCCATACCGCATTCCGCCAATACGATCCC
GACTTGATGGCGGTAACCGGGGAGGGTGCCGGCCACGTGGCCGTGGCTGCTGCCCAGATTGGCGCCCGCCTGATCCATAT
GTCGAGCGATGTCATTTTTGACGGAGAAAAGGGCGAACCATACACAGAAGATGATCCGCCCAATCCAATCACCGATTATG
GTCGCGCAAAAGCGCGGGCTGAAGCGTTGGTGCAGGCAGCATATCCGGCGGCAGCCATTGTGCGCACGTCGCTCATTTAC
GGTTTTGAGCCGATGGATCGCCATACGGCTTTTGCTCTGGCCGTGGCACGCGGAGAGCGCCCGGAACGGTTGTTTCGTGA
TGAGTTCCGTTGTCCGATCTTTGTTGATGATCTGGCCGTAGCCCTGATCGATCTGGCGCAGCGGGATTACCGTGGAGTGA
TCAACCTTGCCGGCGCCGAGATACTCAGCCGCTATGAGTGGGGCTGCCTGCTGGCGCAGGCTTACGGTCTCGATCCAGGT
CTCATACGTGGTGCCTGGAGTGCCGAGAGTCCAACACCACGACCACGCAACTGCGCGCTCGACATCAGCCGGGCGCGTCA
GCTCGGTTTTCGCCTGCGGGGAGCGCGCGAAGTGTTACAGTCGCCTGGTCGGCTCTAA

Upstream 100 bases:

>100_bases
AGCGCGCGGCTGCACGTAAAGCGTTGGCTGCCTGGGATAAGCACGGCTTCACTGCAACGGCCATAGACGCTCAGGATGAG
CGCAACTGAAGCATACCACG

Downstream 100 bases:

>100_bases
CCCTGAAACTCTGTAATCACCGTCACCCCAGAACCACGGAAACTATCGAGATCGACCAGCGCCGCCGGTGCTTCACTCAG
GCTAATCGTGCGGCCAATCA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNGERYLITGGTGYLGQALIRHARCLGVQIAATFHSQTPPALAEVAWHPLDLRDAAAVMALIREVQPTVIVHTAFRQYDP
DLMAVTGEGAGHVAVAAAQIGARLIHMSSDVIFDGEKGEPYTEDDPPNPITDYGRAKARAEALVQAAYPAAAIVRTSLIY
GFEPMDRHTAFALAVARGERPERLFRDEFRCPIFVDDLAVALIDLAQRDYRGVINLAGAEILSRYEWGCLLAQAYGLDPG
LIRGAWSAESPTPRPRNCALDISRARQLGFRLRGAREVLQSPGRL

Sequences:

>Translated_285_residues
MNGERYLITGGTGYLGQALIRHARCLGVQIAATFHSQTPPALAEVAWHPLDLRDAAAVMALIREVQPTVIVHTAFRQYDP
DLMAVTGEGAGHVAVAAAQIGARLIHMSSDVIFDGEKGEPYTEDDPPNPITDYGRAKARAEALVQAAYPAAAIVRTSLIY
GFEPMDRHTAFALAVARGERPERLFRDEFRCPIFVDDLAVALIDLAQRDYRGVINLAGAEILSRYEWGCLLAQAYGLDPG
LIRGAWSAESPTPRPRNCALDISRARQLGFRLRGAREVLQSPGRL
>Mature_285_residues
MNGERYLITGGTGYLGQALIRHARCLGVQIAATFHSQTPPALAEVAWHPLDLRDAAAVMALIREVQPTVIVHTAFRQYDP
DLMAVTGEGAGHVAVAAAQIGARLIHMSSDVIFDGEKGEPYTEDDPPNPITDYGRAKARAEALVQAAYPAAAIVRTSLIY
GFEPMDRHTAFALAVARGERPERLFRDEFRCPIFVDDLAVALIDLAQRDYRGVINLAGAEILSRYEWGCLLAQAYGLDPG
LIRGAWSAESPTPRPRNCALDISRARQLGFRLRGAREVLQSPGRL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=287, Percent_Identity=30.3135888501742, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI11034825, Length=287, Percent_Identity=30.3135888501742, Blast_Score=111, Evalue=6e-25,
Organism=Escherichia coli, GI1788352, Length=283, Percent_Identity=27.208480565371, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31099; Mature: 31099

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGERYLITGGTGYLGQALIRHARCLGVQIAATFHSQTPPALAEVAWHPLDLRDAAAVMA
CCCCEEEEECCCCHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHH
LIREVQPTVIVHTAFRQYDPDLMAVTGEGAGHVAVAAAQIGARLIHMSSDVIFDGEKGEP
HHHHCCCCEEEEEHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHEEECCCEEEECCCCCC
YTEDDPPNPITDYGRAKARAEALVQAAYPAAAIVRTSLIYGFEPMDRHTAFALAVARGER
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
PERLFRDEFRCPIFVDDLAVALIDLAQRDYRGVINLAGAEILSRYEWGCLLAQAYGLDPG
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LIRGAWSAESPTPRPRNCALDISRARQLGFRLRGAREVLQSPGRL
HEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNGERYLITGGTGYLGQALIRHARCLGVQIAATFHSQTPPALAEVAWHPLDLRDAAAVMA
CCCCEEEEECCCCHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHH
LIREVQPTVIVHTAFRQYDPDLMAVTGEGAGHVAVAAAQIGARLIHMSSDVIFDGEKGEP
HHHHCCCCEEEEEHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHEEECCCEEEECCCCCC
YTEDDPPNPITDYGRAKARAEALVQAAYPAAAIVRTSLIYGFEPMDRHTAFALAVARGER
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
PERLFRDEFRCPIFVDDLAVALIDLAQRDYRGVINLAGAEILSRYEWGCLLAQAYGLDPG
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LIRGAWSAESPTPRPRNCALDISRARQLGFRLRGAREVLQSPGRL
HEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]