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The map label for this gene is ybbD [H]

Identifier: 222524491

GI number: 222524491

Start: 1549963

End: 1551510

Strand: Direct

Name: ybbD [H]

Synonym: Chy400_1215

Alternate gene names: 222524491

Gene position: 1549963-1551510 (Clockwise)

Preceding gene: 222524490

Following gene: 222524492

Centisome position: 29.42

GC content: 52.45

Gene sequence:

>1548_bases
ATGAATGCATTGAGTGAACAACTACTAATCTGGTTCGCCGGTGATGAAGTACCATCACATCTGCCGGACCTGATCTTCAA
CCATCGCGTTGCCGGTGTGACGCTCTATCGCCACCTCAATGGCGCATCACCAGCCCGTATCCGTGCATTAACCGCGCATT
TACAGCAACTGGCAGCAGCCTCGGGCTACGGTCCTCTCTTAATCGGTGCCGATCAGGAGGGTGGTCAATTGCAAGCCTTA
CCGGGATTAACCGCTTTTCCCGGTGCAATGGCATTAGGAGCAACCCAATCGCCAGCTTTGGCCTTTCGTGTTGGCAGTGC
AATCGGGGCGGAACTGGCAGCACTTGGGGTTAACCTGGTCTATGCCCCGGTTTGTGATGTTAATACGAACCCACAAAACC
CGGTCATCGGGATCCGTGCCTTTGGCGATGACCCCAAGATCGTCGGAGTACTGGCTGCGGCAATGATTGCCGGCCTGCAG
CAGCATGGAGTTGCCGCAACAGCCAAGCACTTTCCCGGCCACGGAGATACGTTTGGTGATAGTCATTATGGTCTGGTTAG
GGTTAATCACGATTACGAACGATTACAGCACGTAGAATGGTTGCCATTTTCGGCGGCTATCGAGGCTGGTGTTCAGGCAG
TGATGGTTGGACATCTGGCTGTACCTGCATTGCAGGCAGCGGATCGCCCTGCCACCCGCGCACCACAGGTCATAGCGCAT
CTGCGGCATCACCTCGGATTCAGAGGTGTTACGATTAGCGATGCACTCGACATGAAAGCCTTTGCACCTGACGATGGATA
TGCCAGAGCTTTGTCGGAAGCAGTTCATGCAGGTATCGATCTGCTCTTGCTCGGTGCAGGAAATGATCTGGAGCAGACAA
TAAAGTTTCTCAGTACAATTGATAGAAACACTAAAAGTACTATTTCCAGCCAGCGCATTGCGGCGCTGCGCCAATGGCTG
CGGAATCGTCCTCAACCAGATCTATCAGTTGTCGGATGTGCAGCTCATCACGCGCTGGCAGATGAGGTGGCGCAGCGCGC
ATTAACCCTGGTGCGCAATCAGGAAGGCTTATTACCACTACAATTATCAACAACAGCCCGTGTCGTGGTAATCATGCCGC
AACCCGTTGATCTTACCCCTGCCGACACATCAGCAAGTGAACGGCCTGCACTTGCCGAGGCGATCCTTTCTTGCCATTCT
TATGTTCAGTCCTTCATTATCGATATTGATCCCTCTCCAGCCAGCATTGCCGATATTCTAGCTGCTCTCGCCGAAGCCGA
TCTGGTGATCATAGGAACAATCAACGCCCAAACGTATCGTGGTCAGGCTTCGCTCGTACACGAGATCTTTCAAAGAGGCA
TTCCCCTGATTACCGTAGCATTACGTCTCCCCACCGATTTGCTCGCCTACCCGGAAGTGTCAACGCATCTCTGTACTTAT
AGTTTGCAACCGGTAGCCTTGAAAGCACTTGCTGCTGGCCTATGGGGTGAAATTCCATTCCAGGGTAAGCTACCATTACG
AGTATTGTCTGAGGAATTTAATGTATGA

Upstream 100 bases:

>100_bases
TAGCCCCGCCGCAACCCTTGACAGATCGAACCTTACACATTCATTGTGGACCAAAGGAAATTCAAAGCTGGTGGTTCATC
ATTGGTGAGGAGGCGACCTA

Downstream 100 bases:

>100_bases
CCATTCAACACGAAATTTACGCACAACCCACAGTTATTAGTGAACTACTTGAACATGGGTTGGTACAGGCATACCAATTG
GCCGCTAAAATTCGCAAGCA

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNALSEQLLIWFAGDEVPSHLPDLIFNHRVAGVTLYRHLNGASPARIRALTAHLQQLAAASGYGPLLIGADQEGGQLQAL
PGLTAFPGAMALGATQSPALAFRVGSAIGAELAALGVNLVYAPVCDVNTNPQNPVIGIRAFGDDPKIVGVLAAAMIAGLQ
QHGVAATAKHFPGHGDTFGDSHYGLVRVNHDYERLQHVEWLPFSAAIEAGVQAVMVGHLAVPALQAADRPATRAPQVIAH
LRHHLGFRGVTISDALDMKAFAPDDGYARALSEAVHAGIDLLLLGAGNDLEQTIKFLSTIDRNTKSTISSQRIAALRQWL
RNRPQPDLSVVGCAAHHALADEVAQRALTLVRNQEGLLPLQLSTTARVVVIMPQPVDLTPADTSASERPALAEAILSCHS
YVQSFIIDIDPSPASIADILAALAEADLVIIGTINAQTYRGQASLVHEIFQRGIPLITVALRLPTDLLAYPEVSTHLCTY
SLQPVALKALAAGLWGEIPFQGKLPLRVLSEEFNV

Sequences:

>Translated_515_residues
MNALSEQLLIWFAGDEVPSHLPDLIFNHRVAGVTLYRHLNGASPARIRALTAHLQQLAAASGYGPLLIGADQEGGQLQAL
PGLTAFPGAMALGATQSPALAFRVGSAIGAELAALGVNLVYAPVCDVNTNPQNPVIGIRAFGDDPKIVGVLAAAMIAGLQ
QHGVAATAKHFPGHGDTFGDSHYGLVRVNHDYERLQHVEWLPFSAAIEAGVQAVMVGHLAVPALQAADRPATRAPQVIAH
LRHHLGFRGVTISDALDMKAFAPDDGYARALSEAVHAGIDLLLLGAGNDLEQTIKFLSTIDRNTKSTISSQRIAALRQWL
RNRPQPDLSVVGCAAHHALADEVAQRALTLVRNQEGLLPLQLSTTARVVVIMPQPVDLTPADTSASERPALAEAILSCHS
YVQSFIIDIDPSPASIADILAALAEADLVIIGTINAQTYRGQASLVHEIFQRGIPLITVALRLPTDLLAYPEVSTHLCTY
SLQPVALKALAAGLWGEIPFQGKLPLRVLSEEFNV
>Mature_515_residues
MNALSEQLLIWFAGDEVPSHLPDLIFNHRVAGVTLYRHLNGASPARIRALTAHLQQLAAASGYGPLLIGADQEGGQLQAL
PGLTAFPGAMALGATQSPALAFRVGSAIGAELAALGVNLVYAPVCDVNTNPQNPVIGIRAFGDDPKIVGVLAAAMIAGLQ
QHGVAATAKHFPGHGDTFGDSHYGLVRVNHDYERLQHVEWLPFSAAIEAGVQAVMVGHLAVPALQAADRPATRAPQVIAH
LRHHLGFRGVTISDALDMKAFAPDDGYARALSEAVHAGIDLLLLGAGNDLEQTIKFLSTIDRNTKSTISSQRIAALRQWL
RNRPQPDLSVVGCAAHHALADEVAQRALTLVRNQEGLLPLQLSTTARVVVIMPQPVDLTPADTSASERPALAEAILSCHS
YVQSFIIDIDPSPASIADILAALAEADLVIIGTINAQTYRGQASLVHEIFQRGIPLITVALRLPTDLLAYPEVSTHLCTY
SLQPVALKALAAGLWGEIPFQGKLPLRVLSEEFNV

Specific function: Hydrolyses the beta-1,4-glycosidic bond between N-acetylglucosamine and anhydro-N-acetylmuramic acid in cell wall degradation products following their importation into the cell during the process for recycling cell wall muropeptides [C]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=310, Percent_Identity=29.3548387096774, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788453, Length=361, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR002772
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3; PF01915 Glyco_hydro_3_C [H]

EC number: 3.2.1.52

Molecular weight: Translated: 54645; Mature: 54645

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALSEQLLIWFAGDEVPSHLPDLIFNHRVAGVTLYRHLNGASPARIRALTAHLQQLAAA
CCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHHHH
SGYGPLLIGADQEGGQLQALPGLTAFPGAMALGATQSPALAFRVGSAIGAELAALGVNLV
CCCCCEEEECCCCCCEEEECCCCCCCCCHHCCCCCCCCCEEEEHHHHHHHHHHHHCCEEE
YAPVCDVNTNPQNPVIGIRAFGDDPKIVGVLAAAMIAGLQQHGVAATAKHFPGHGDTFGD
EEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCC
SHYGLVRVNHDYERLQHVEWLPFSAAIEAGVQAVMVGHLAVPALQAADRPATRAPQVIAH
CCEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LRHHLGFRGVTISDALDMKAFAPDDGYARALSEAVHAGIDLLLLGAGNDLEQTIKFLSTI
HHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
DRNTKSTISSQRIAALRQWLRNRPQPDLSVVGCAAHHALADEVAQRALTLVRNQEGLLPL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
QLSTTARVVVIMPQPVDLTPADTSASERPALAEAILSCHSYVQSFIIDIDPSPASIADIL
EECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHH
AALAEADLVIIGTINAQTYRGQASLVHEIFQRGIPLITVALRLPTDLLAYPEVSTHLCTY
HHHHCCCEEEEEECCCCEECCHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHEEE
SLQPVALKALAAGLWGEIPFQGKLPLRVLSEEFNV
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MNALSEQLLIWFAGDEVPSHLPDLIFNHRVAGVTLYRHLNGASPARIRALTAHLQQLAAA
CCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHHHH
SGYGPLLIGADQEGGQLQALPGLTAFPGAMALGATQSPALAFRVGSAIGAELAALGVNLV
CCCCCEEEECCCCCCEEEECCCCCCCCCHHCCCCCCCCCEEEEHHHHHHHHHHHHCCEEE
YAPVCDVNTNPQNPVIGIRAFGDDPKIVGVLAAAMIAGLQQHGVAATAKHFPGHGDTFGD
EEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCC
SHYGLVRVNHDYERLQHVEWLPFSAAIEAGVQAVMVGHLAVPALQAADRPATRAPQVIAH
CCEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LRHHLGFRGVTISDALDMKAFAPDDGYARALSEAVHAGIDLLLLGAGNDLEQTIKFLSTI
HHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
DRNTKSTISSQRIAALRQWLRNRPQPDLSVVGCAAHHALADEVAQRALTLVRNQEGLLPL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
QLSTTARVVVIMPQPVDLTPADTSASERPALAEAILSCHSYVQSFIIDIDPSPASIADIL
EECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHH
AALAEADLVIIGTINAQTYRGQASLVHEIFQRGIPLITVALRLPTDLLAYPEVSTHLCTY
HHHHCCCEEEEEECCCCEECCHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHEEE
SLQPVALKALAAGLWGEIPFQGKLPLRVLSEEFNV
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8011666; 9274029; 9384377 [H]