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The map label for this gene is est [H]

Identifier: 222524432

GI number: 222524432

Start: 1465179

End: 1465964

Strand: Direct

Name: est [H]

Synonym: Chy400_1156

Alternate gene names: 222524432

Gene position: 1465179-1465964 (Clockwise)

Preceding gene: 222524431

Following gene: 222524436

Centisome position: 27.81

GC content: 60.56

Gene sequence:

>786_bases
ATGCTTGAACCGTATTTGTTGTCGAGTGGTGATCACGCTTGCTTGCTGATCCACGGGTTTGTCGGCGATCCCGGTGAGAT
GCGCGATCTAGGCGATCATCTGGCGCAAGCCGGCTATACGGTATTTGGTATGCGTCTGCCCGGACATACCGGGAACCCTG
AAGACCTGATTGCTGTGCGCTGGACGGACTGGCTGGCTGCGGTGCAGGCGGCATTTGACGCACTCGCCCGCCGGTCTACT
GCTGTCTCAGTAGTGGGTTTTTCATTGGGTGGCGCACTGGCCGCGTTGCTGGCAGCACAGCGCCCTGTGCACCGCCTGGT
GATGCTGGCAACACCCTACCGCCTTGGTGGCGACTGGCGGGTTGATCTGTTGGGGATTGTCCGCTACGTGACCCCCTGGT
TTTATCTGCTGGCCCAGGCCGATTTCAGCAGCCCCGATCTACGGGCATCAATCTGGCGTCGCCAGCCCGGCCTTGATCTC
GACGATCCGGTCATTCAACAGATGCTACGACGCTCGGTGAAGGTGTCAGTCGCCGCCGCTGATGAGTTGCGGCTGACTCT
GGCAGCCGCTCGTCGTGCACTGCCGCTGGTACGGGCACCAACCCTGATTATGCACGGTCGCAGTGATACAACAGCCGATC
CAGCCAGTGCCGCAGCTATCATGGCTCAGATCGGCAGTACTTACCGTGAACTCACATACTGGCCGGCCACCGGTCATCAA
CTGCTACTCACCGGGCCGTACCGGTCGGCCATCTTTCAACGGATCACACGGTTTCTCGCCCGCTAG

Upstream 100 bases:

>100_bases
GCTCACGGGCCGTTCAAGAGTGTTGATGATCTGACGGCCATTTCCGGTATTGGCGAGCGTAATATCAATATCTTCCGTAA
GCTGGTATACGTTGATCCCA

Downstream 100 bases:

>100_bases
CCTTTCCCTGCTGCCTGGAACGTATCGTAAACGACTCTACTCTCGCTTGATAACGATCAGCGTAATGTCATCAAACGGGG
GCTGACCGTGGCTATGGCGT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLEPYLLSSGDHACLLIHGFVGDPGEMRDLGDHLAQAGYTVFGMRLPGHTGNPEDLIAVRWTDWLAAVQAAFDALARRST
AVSVVGFSLGGALAALLAAQRPVHRLVMLATPYRLGGDWRVDLLGIVRYVTPWFYLLAQADFSSPDLRASIWRRQPGLDL
DDPVIQQMLRRSVKVSVAAADELRLTLAAARRALPLVRAPTLIMHGRSDTTADPASAAAIMAQIGSTYRELTYWPATGHQ
LLLTGPYRSAIFQRITRFLAR

Sequences:

>Translated_261_residues
MLEPYLLSSGDHACLLIHGFVGDPGEMRDLGDHLAQAGYTVFGMRLPGHTGNPEDLIAVRWTDWLAAVQAAFDALARRST
AVSVVGFSLGGALAALLAAQRPVHRLVMLATPYRLGGDWRVDLLGIVRYVTPWFYLLAQADFSSPDLRASIWRRQPGLDL
DDPVIQQMLRRSVKVSVAAADELRLTLAAARRALPLVRAPTLIMHGRSDTTADPASAAAIMAQIGSTYRELTYWPATGHQ
LLLTGPYRSAIFQRITRFLAR
>Mature_261_residues
MLEPYLLSSGDHACLLIHGFVGDPGEMRDLGDHLAQAGYTVFGMRLPGHTGNPEDLIAVRWTDWLAAVQAAFDALARRST
AVSVVGFSLGGALAALLAAQRPVHRLVMLATPYRLGGDWRVDLLGIVRYVTPWFYLLAQADFSSPDLRASIWRRQPGLDL
DDPVIQQMLRRSVKVSVAAADELRLTLAAARRALPLVRAPTLIMHGRSDTTADPASAAAIMAQIGSTYRELTYWPATGHQ
LLLTGPYRSAIFQRITRFLAR

Specific function: Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones [H]

COG id: COG1647

COG function: function code R; Esterase/lipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012354
- InterPro:   IPR001375 [H]

Pfam domain/function: PF00326 Peptidase_S9 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28597; Mature: 28597

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPYLLSSGDHACLLIHGFVGDPGEMRDLGDHLAQAGYTVFGMRLPGHTGNPEDLIAVR
CCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEE
WTDWLAAVQAAFDALARRSTAVSVVGFSLGGALAALLAAQRPVHRLVMLATPYRLGGDWR
HHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCH
VDLLGIVRYVTPWFYLLAQADFSSPDLRASIWRRQPGLDLDDPVIQQMLRRSVKVSVAAA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEEH
DELRLTLAAARRALPLVRAPTLIMHGRSDTTADPASAAAIMAQIGSTYRELTYWPATGHQ
HHHHHHHHHHHHHCCHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHEEECCCCCCE
LLLTGPYRSAIFQRITRFLAR
EEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEPYLLSSGDHACLLIHGFVGDPGEMRDLGDHLAQAGYTVFGMRLPGHTGNPEDLIAVR
CCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEE
WTDWLAAVQAAFDALARRSTAVSVVGFSLGGALAALLAAQRPVHRLVMLATPYRLGGDWR
HHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCH
VDLLGIVRYVTPWFYLLAQADFSSPDLRASIWRRQPGLDLDDPVIQQMLRRSVKVSVAAA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEEH
DELRLTLAAARRALPLVRAPTLIMHGRSDTTADPASAAAIMAQIGSTYRELTYWPATGHQ
HHHHHHHHHHHHHCCHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHEEECCCCCCE
LLLTGPYRSAIFQRITRFLAR
EEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1369099 [H]