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The map label for this gene is oppA [H]

Identifier: 222524400

GI number: 222524400

Start: 1421446

End: 1423230

Strand: Direct

Name: oppA [H]

Synonym: Chy400_1124

Alternate gene names: 222524400

Gene position: 1421446-1423230 (Clockwise)

Preceding gene: 222524399

Following gene: 222524401

Centisome position: 26.98

GC content: 57.59

Gene sequence:

>1785_bases
ATGAATTTGCTTTACTGGCGTCGCTCGCTCGCACTGGCTCTCCTGTTTGGGTTACTCTTACCCATCCTCGCAGCCTGTGG
CGGTGGTTCCACGCCGGCAGCTCAACCGACAACGGCCCCCGCCGAGCAACCAACAACCGCTCCCGCCGAGCAACCAACAA
CCGCCCCCGCTGAACAACCAACTGCCGCGCCGGTTGCCGGCACTGCGCAACCCGGCGTTCTGCGCCTCGTGACCGGTTCT
GAGCCGGAGAACATCGATCCACAAAAATCGAGCTTCGTCAACGAGATTCAGTTCATCATGATGGCCTATCAGGCGCTGAT
GACCTTCGATCTCGATATGAATCCTATTCCTGGAGCTGCCGAAAAGGTTGAGGTTTCAGAAGACGGTACCGTCTACACAT
TCACCTTACGGCGCGATGGCAAGTACAGTGATGGGACACCGCTGACGGCACGTAACTTTGAATACGCCTGGAAGCGTCTG
GCCGATCCTGAGACTGCCGGTGAGTACCAGTCGCTGCCGTGTGGCATCATCAAAGGCTACAGCGAGTACAGTGCCGCTGC
GTGCCAGGGTCTGTCGATGGAAGAGGTCATGCAGCTCGATCTCGAGAAGCTGCGCGACGAATTTGGCGTCAAGGCCATCG
ATGACTACACGCTGCAGATCGAGCTGGTCGCACCTGCTCCCTACTTCCTCAGCATGGCAGCCCTCTGGATTGGCGCACCG
GTGCGTGAGGAGGATGTTGCGAAGGGTGACGACTGGTGGTACTACCCGGAAAACTACATCGGTAACGGCCCGTTTGTGCT
CAAGGAGTGGGAGCACGACAGCAAGGCAGTGTGGGAGCCTAACCCCAATTACGTCGGTCCGCTGGGTCCGGTCAAGCTCA
AGCGGGTTGAGTACTACATGATCACCGACGGTCAGGTAGCGTTCCAGGCTTATCAGAACGGTGAGATTGACATCCTCGGT
GTGGGTGCCGAAGACCTGGCAACCGTTCAATCTGATCCCGTCTTGAGCAAGGAAGTAATTGACGTACCCGGTTCGTGTAC
CTTCTACTTCGGCTTCAACCTGGCGAAGCCACCGTTTGACAACAAACTGGTGCGGCAGGCATTTGCCCAGGCGCTCGACC
GTGAAGCGTATGTACGCGATGTCTTCCAGGGTCTGGGGACACCGACCTACAGCTTTATTCCACCCGGTTTTCCTGGGTAC
CAGCCTGATCTGAAGCTGTGGGAGTTTAACCCGGAGAAGGCGAAGCAGACGCTCGCCGAAGCCGGCTATCCGGATGGGCA
GGGGCTGCCTGAAATCCGGCTTACCTATTCAGGTACGGCACGTAACAATGCTCGCTTCGAGTGGGTAGCCAACCAGTTCA
AGCAGAATCTGGGGATCGATGTGATCCTTGATCCGATTGATCCGACAGCCTTTGCTGCGGCCACCAAGGACAATCCGCCG
CAAATGTTCTCGCTCGGCTGGTGCGCCGACTACCCCGATCCGCAGAACTGGATCTCGTTGTTCCAGACCAACGGTCTGCT
CAGCGCACGGGTGAACTACTCGAACCCGCAACTTGATGAGCTGGTGCGCCAGGCTGACGTTGAGCAAGACCCGGTGAAGC
GGGCCGAACTGTACGCTCAGGCGCAGAGGATTCTGGTCGAAGATGCACCGGTGGTCTTTATGCTCAACAACGGTGGGCCG
GTACTGGTGAAGCCATACGTGAAGGGTATCACACCTGACACCATCACCCCACTCGATTACTGGCTTGGCTTCTTCAACCT
GCCCAACGTTGATGTCCAACCATAG

Upstream 100 bases:

>100_bases
CACCCAAAAACCGTTTCTGTCCGGCGTAGTGCGGCTTTGCCGGTGTTCCTCGCTTCGAGTGTTTTGTTCGCCTGACAGAT
ACCTTTCAAGGAGTGTTGCG

Downstream 100 bases:

>100_bases
TCGGCGTTCCTGAAGCCCTATCGGTGGCGCGAGATAACCTGCTCGCGCCACCAGAGCATGCCTATGACAGCCTATATCAT
TCGGCGCATTCTGTGGAATA

Product: family 5 extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MNLLYWRRSLALALLFGLLLPILAACGGGSTPAAQPTTAPAEQPTTAPAEQPTTAPAEQPTAAPVAGTAQPGVLRLVTGS
EPENIDPQKSSFVNEIQFIMMAYQALMTFDLDMNPIPGAAEKVEVSEDGTVYTFTLRRDGKYSDGTPLTARNFEYAWKRL
ADPETAGEYQSLPCGIIKGYSEYSAAACQGLSMEEVMQLDLEKLRDEFGVKAIDDYTLQIELVAPAPYFLSMAALWIGAP
VREEDVAKGDDWWYYPENYIGNGPFVLKEWEHDSKAVWEPNPNYVGPLGPVKLKRVEYYMITDGQVAFQAYQNGEIDILG
VGAEDLATVQSDPVLSKEVIDVPGSCTFYFGFNLAKPPFDNKLVRQAFAQALDREAYVRDVFQGLGTPTYSFIPPGFPGY
QPDLKLWEFNPEKAKQTLAEAGYPDGQGLPEIRLTYSGTARNNARFEWVANQFKQNLGIDVILDPIDPTAFAAATKDNPP
QMFSLGWCADYPDPQNWISLFQTNGLLSARVNYSNPQLDELVRQADVEQDPVKRAELYAQAQRILVEDAPVVFMLNNGGP
VLVKPYVKGITPDTITPLDYWLGFFNLPNVDVQP

Sequences:

>Translated_594_residues
MNLLYWRRSLALALLFGLLLPILAACGGGSTPAAQPTTAPAEQPTTAPAEQPTTAPAEQPTAAPVAGTAQPGVLRLVTGS
EPENIDPQKSSFVNEIQFIMMAYQALMTFDLDMNPIPGAAEKVEVSEDGTVYTFTLRRDGKYSDGTPLTARNFEYAWKRL
ADPETAGEYQSLPCGIIKGYSEYSAAACQGLSMEEVMQLDLEKLRDEFGVKAIDDYTLQIELVAPAPYFLSMAALWIGAP
VREEDVAKGDDWWYYPENYIGNGPFVLKEWEHDSKAVWEPNPNYVGPLGPVKLKRVEYYMITDGQVAFQAYQNGEIDILG
VGAEDLATVQSDPVLSKEVIDVPGSCTFYFGFNLAKPPFDNKLVRQAFAQALDREAYVRDVFQGLGTPTYSFIPPGFPGY
QPDLKLWEFNPEKAKQTLAEAGYPDGQGLPEIRLTYSGTARNNARFEWVANQFKQNLGIDVILDPIDPTAFAAATKDNPP
QMFSLGWCADYPDPQNWISLFQTNGLLSARVNYSNPQLDELVRQADVEQDPVKRAELYAQAQRILVEDAPVVFMLNNGGP
VLVKPYVKGITPDTITPLDYWLGFFNLPNVDVQP
>Mature_594_residues
MNLLYWRRSLALALLFGLLLPILAACGGGSTPAAQPTTAPAEQPTTAPAEQPTTAPAEQPTAAPVAGTAQPGVLRLVTGS
EPENIDPQKSSFVNEIQFIMMAYQALMTFDLDMNPIPGAAEKVEVSEDGTVYTFTLRRDGKYSDGTPLTARNFEYAWKRL
ADPETAGEYQSLPCGIIKGYSEYSAAACQGLSMEEVMQLDLEKLRDEFGVKAIDDYTLQIELVAPAPYFLSMAALWIGAP
VREEDVAKGDDWWYYPENYIGNGPFVLKEWEHDSKAVWEPNPNYVGPLGPVKLKRVEYYMITDGQVAFQAYQNGEIDILG
VGAEDLATVQSDPVLSKEVIDVPGSCTFYFGFNLAKPPFDNKLVRQAFAQALDREAYVRDVFQGLGTPTYSFIPPGFPGY
QPDLKLWEFNPEKAKQTLAEAGYPDGQGLPEIRLTYSGTARNNARFEWVANQFKQNLGIDVILDPIDPTAFAAATKDNPP
QMFSLGWCADYPDPQNWISLFQTNGLLSARVNYSNPQLDELVRQADVEQDPVKRAELYAQAQRILVEDAPVVFMLNNGGP
VLVKPYVKGITPDTITPLDYWLGFFNLPNVDVQP

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=518, Percent_Identity=32.046332046332, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI87081878, Length=506, Percent_Identity=29.8418972332016, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1789397, Length=503, Percent_Identity=29.8210735586481, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789966, Length=557, Percent_Identity=25.8527827648115, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787052, Length=520, Percent_Identity=26.3461538461538, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1787762, Length=536, Percent_Identity=24.4402985074627, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1789887, Length=486, Percent_Identity=24.4855967078189, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787551, Length=525, Percent_Identity=22.6666666666667, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 65641; Mature: 65641

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLYWRRSLALALLFGLLLPILAACGGGSTPAAQPTTAPAEQPTTAPAEQPTTAPAEQP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TAAPVAGTAQPGVLRLVTGSEPENIDPQKSSFVNEIQFIMMAYQALMTFDLDMNPIPGAA
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EKVEVSEDGTVYTFTLRRDGKYSDGTPLTARNFEYAWKRLADPETAGEYQSLPCGIIKGY
CEEEECCCCEEEEEEEEECCCCCCCCCCEECCHHHHHHHHCCCCCCCCCCCCCHHHHCCC
SEYSAAACQGLSMEEVMQLDLEKLRDEFGVKAIDDYTLQIELVAPAPYFLSMAALWIGAP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCEEEEEEEECCCHHHHHHHHHHHCCC
VREEDVAKGDDWWYYPENYIGNGPFVLKEWEHDSKAVWEPNPNYVGPLGPVKLKRVEYYM
CCHHHCCCCCCEEECCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCEEEEEEEEEE
ITDGQVAFQAYQNGEIDILGVGAEDLATVQSDPVLSKEVIDVPGSCTFYFGFNLAKPPFD
EECCEEEEEEECCCCEEEEEECHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCC
NKLVRQAFAQALDREAYVRDVFQGLGTPTYSFIPPGFPGYQPDLKLWEFNPEKAKQTLAE
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
AGYPDGQGLPEIRLTYSGTARNNARFEWVANQFKQNLGIDVILDPIDPTAFAAATKDNPP
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCC
QMFSLGWCADYPDPQNWISLFQTNGLLSARVNYSNPQLDELVRQADVEQDPVKRAELYAQ
CEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHH
AQRILVEDAPVVFMLNNGGPVLVKPYVKGITPDTITPLDYWLGFFNLPNVDVQP
HHHHHEECCCEEEEECCCCCEEEEHHHCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNLLYWRRSLALALLFGLLLPILAACGGGSTPAAQPTTAPAEQPTTAPAEQPTTAPAEQP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TAAPVAGTAQPGVLRLVTGSEPENIDPQKSSFVNEIQFIMMAYQALMTFDLDMNPIPGAA
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EKVEVSEDGTVYTFTLRRDGKYSDGTPLTARNFEYAWKRLADPETAGEYQSLPCGIIKGY
CEEEECCCCEEEEEEEEECCCCCCCCCCEECCHHHHHHHHCCCCCCCCCCCCCHHHHCCC
SEYSAAACQGLSMEEVMQLDLEKLRDEFGVKAIDDYTLQIELVAPAPYFLSMAALWIGAP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCEEEEEEEECCCHHHHHHHHHHHCCC
VREEDVAKGDDWWYYPENYIGNGPFVLKEWEHDSKAVWEPNPNYVGPLGPVKLKRVEYYM
CCHHHCCCCCCEEECCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCEEEEEEEEEE
ITDGQVAFQAYQNGEIDILGVGAEDLATVQSDPVLSKEVIDVPGSCTFYFGFNLAKPPFD
EECCEEEEEEECCCCEEEEEECHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCC
NKLVRQAFAQALDREAYVRDVFQGLGTPTYSFIPPGFPGYQPDLKLWEFNPEKAKQTLAE
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
AGYPDGQGLPEIRLTYSGTARNNARFEWVANQFKQNLGIDVILDPIDPTAFAAATKDNPP
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCC
QMFSLGWCADYPDPQNWISLFQTNGLLSARVNYSNPQLDELVRQADVEQDPVKRAELYAQ
CEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHH
AQRILVEDAPVVFMLNNGGPVLVKPYVKGITPDTITPLDYWLGFFNLPNVDVQP
HHHHHEECCCEEEEECCCCCEEEEHHHCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]