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The map label for this gene is kinE [H]

Identifier: 222524324

GI number: 222524324

Start: 1331554

End: 1333002

Strand: Direct

Name: kinE [H]

Synonym: Chy400_1047

Alternate gene names: 222524324

Gene position: 1331554-1333002 (Clockwise)

Preceding gene: 222524318

Following gene: 222524327

Centisome position: 25.27

GC content: 54.59

Gene sequence:

>1449_bases
ATGTCTGGCTTACTACCTGCGAGAGGAGACGCGCACATGATAGAGCGGGAAGATACGGTTGCATACGTTGCCAGGTTGGA
GCGGGAATTGCAGGCTGCTCAGGATCGAATTGTAGCACTTGAACGTGAAGCTACGCTCCATGCTCAGTACGCGCGTCATC
TTGATCAACGGCTGACAGCACTCTTGTCAATCTCGGCGGCTCTGCTTATCAGTCGCGATGTTGAAACCATTGTCAAACTG
GTGGTGCAACATGCGACAAGTCTCTTCCCCGGAACTGCGGCTGCGTATCTCTACCTACTCGACGGCAGCAGTGAGAGTAC
TATTCAACTGAAAGCATCAATTCCAGATCACACCCCTTCTGCCCTGATAACGACACTGGCCGAAATGGCGATTAGGGCAC
CACGGGCAATGTTATTGAATGGGCCAGAATTAGAACGGCTTATCGATCAGCAACCGGCCTCAGACGGTTCCATCAATGGC
GATTGTGCAACGCCACAAAGTATTTTACTGGCACCACTGCGGATCGAAAACCAGCGCATGGGAGCGCTGATTGTGTGCAG
CGATCAGTTCTATCATTCATTTTTGCCACGCGATCTGCCATTTGCACAGGCGCTGGCCGATCTGACAATGATTGCGATTG
ACGAGATTCAGCAACGCCAGCGCGCTGCGGCACTACAACGCGACCTGGTACATACGCAATTGCTCCGGGCCGAGGCTGAA
GCCCGTCTGAATGCGGCGCAGGCTCAATTGCTCCAAAGTGCCAAGCTGGCCGCCGTCGGTGAACTGGCTGCTTCGGTGGC
ACACGAGATCAATAATCCGCTTTATGCCGCCCGCAACAGTCTCTATCTGGTAGAGCAAGACCTGCCACCTGACGCACCGC
AACGTACCTTTCTCACCATTGCCCAACAAGAGTTAGGTCGGATTGCCCGCATCATCACCCGCATGCGTGACTTTTACCGT
CCTTCGCGCGGTGAATTGAGCAGCACTAACGTTAATGCCCTTCTGCGCGAGACCCTCGAACTGGTGACCACGCATCTTCG
GCACAATCACGTGGCTGTCCACGCGCATCTGGCAAACGATCTACCTGCCATCACCGCTCACGCCGATCAGTTGCGGCAGG
TCTTTCTCAACGTCATTCTCAATGCCTGTGATGCCATGCCGAATGGTGGCGAATTGACAGTCAAAAGCAGTCTGATCGCT
GCACGACCGGAGGCGCCGGCAGCCCTGGCGATTGCGATCAGTGATACTGGTGTCGGGATTGCTCCAGAACACCTCCCCCA
CCTGTTCGAGCCGTTCTATACTACGAAACCGCATGGCACTGGACTGGGACTGGCAATCAGTGCCCATATTGTGACTCAAC
ACGGCGGTCGAATTCTGGTCGATAGTCAGCCAGGGTCGGGAACCACGTTCACTATTTTACTGCCACTTGAAGTACCAAAG
GTAGAGTGA

Upstream 100 bases:

>100_bases
GGCATATCATAGCATGAGCTGATCGTGTGGTCAACGGTGTACACATCGCACATCATACTACCCGACTATCACACAGCCAA
TGTTTGCGAAGACGTCTGCC

Downstream 100 bases:

>100_bases
GGAAGAATGGAAACACACCCAATGACTCACACGGTTTGATGTAACACCTGGGCAACCAGTGGTACCAGTTCACGCAATTC
AATCGGCTTTGCCGCGTAGA

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSGLLPARGDAHMIEREDTVAYVARLERELQAAQDRIVALEREATLHAQYARHLDQRLTALLSISAALLISRDVETIVKL
VVQHATSLFPGTAAAYLYLLDGSSESTIQLKASIPDHTPSALITTLAEMAIRAPRAMLLNGPELERLIDQQPASDGSING
DCATPQSILLAPLRIENQRMGALIVCSDQFYHSFLPRDLPFAQALADLTMIAIDEIQQRQRAAALQRDLVHTQLLRAEAE
ARLNAAQAQLLQSAKLAAVGELAASVAHEINNPLYAARNSLYLVEQDLPPDAPQRTFLTIAQQELGRIARIITRMRDFYR
PSRGELSSTNVNALLRETLELVTTHLRHNHVAVHAHLANDLPAITAHADQLRQVFLNVILNACDAMPNGGELTVKSSLIA
ARPEAPAALAIAISDTGVGIAPEHLPHLFEPFYTTKPHGTGLGLAISAHIVTQHGGRILVDSQPGSGTTFTILLPLEVPK
VE

Sequences:

>Translated_482_residues
MSGLLPARGDAHMIEREDTVAYVARLERELQAAQDRIVALEREATLHAQYARHLDQRLTALLSISAALLISRDVETIVKL
VVQHATSLFPGTAAAYLYLLDGSSESTIQLKASIPDHTPSALITTLAEMAIRAPRAMLLNGPELERLIDQQPASDGSING
DCATPQSILLAPLRIENQRMGALIVCSDQFYHSFLPRDLPFAQALADLTMIAIDEIQQRQRAAALQRDLVHTQLLRAEAE
ARLNAAQAQLLQSAKLAAVGELAASVAHEINNPLYAARNSLYLVEQDLPPDAPQRTFLTIAQQELGRIARIITRMRDFYR
PSRGELSSTNVNALLRETLELVTTHLRHNHVAVHAHLANDLPAITAHADQLRQVFLNVILNACDAMPNGGELTVKSSLIA
ARPEAPAALAIAISDTGVGIAPEHLPHLFEPFYTTKPHGTGLGLAISAHIVTQHGGRILVDSQPGSGTTFTILLPLEVPK
VE
>Mature_481_residues
SGLLPARGDAHMIEREDTVAYVARLERELQAAQDRIVALEREATLHAQYARHLDQRLTALLSISAALLISRDVETIVKLV
VQHATSLFPGTAAAYLYLLDGSSESTIQLKASIPDHTPSALITTLAEMAIRAPRAMLLNGPELERLIDQQPASDGSINGD
CATPQSILLAPLRIENQRMGALIVCSDQFYHSFLPRDLPFAQALADLTMIAIDEIQQRQRAAALQRDLVHTQLLRAEAEA
RLNAAQAQLLQSAKLAAVGELAASVAHEINNPLYAARNSLYLVEQDLPPDAPQRTFLTIAQQELGRIARIITRMRDFYRP
SRGELSSTNVNALLRETLELVTTHLRHNHVAVHAHLANDLPAITAHADQLRQVFLNVILNACDAMPNGGELTVKSSLIAA
RPEAPAALAIAISDTGVGIAPEHLPHLFEPFYTTKPHGTGLGLAISAHIVTQHGGRILVDSQPGSGTTFTILLPLEVPKV
E

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=35.042735042735, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1788549, Length=230, Percent_Identity=36.9565217391304, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790300, Length=232, Percent_Identity=31.4655172413793, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081816, Length=274, Percent_Identity=31.021897810219, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=26.171875, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=28.8288288288288, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788393, Length=228, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789149, Length=249, Percent_Identity=26.9076305220884, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786783, Length=225, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52340; Mature: 52208

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLLPARGDAHMIEREDTVAYVARLERELQAAQDRIVALEREATLHAQYARHLDQRLTA
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
LLSISAALLISRDVETIVKLVVQHATSLFPGTAAAYLYLLDGSSESTIQLKASIPDHTPS
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCHH
ALITTLAEMAIRAPRAMLLNGPELERLIDQQPASDGSINGDCATPQSILLAPLRIENQRM
HHHHHHHHHHHHCCHHEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHEEECEEECCCCC
GALIVCSDQFYHSFLPRDLPFAQALADLTMIAIDEIQQRQRAAALQRDLVHTQLLRAEAE
CEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLNAAQAQLLQSAKLAAVGELAASVAHEINNPLYAARNSLYLVEQDLPPDAPQRTFLTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCCHHHHHH
AQQELGRIARIITRMRDFYRPSRGELSSTNVNALLRETLELVTTHLRHNHVAVHAHLAND
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
LPAITAHADQLRQVFLNVILNACDAMPNGGELTVKSSLIAARPEAPAALAIAISDTGVGI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECCCCCCEEEEEEEECCCCCC
APEHLPHLFEPFYTTKPHGTGLGLAISAHIVTQHGGRILVDSQPGSGTTFTILLPLEVPK
CHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCEEEEEEEECCCC
VE
CC
>Mature Secondary Structure 
SGLLPARGDAHMIEREDTVAYVARLERELQAAQDRIVALEREATLHAQYARHLDQRLTA
CCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
LLSISAALLISRDVETIVKLVVQHATSLFPGTAAAYLYLLDGSSESTIQLKASIPDHTPS
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCHH
ALITTLAEMAIRAPRAMLLNGPELERLIDQQPASDGSINGDCATPQSILLAPLRIENQRM
HHHHHHHHHHHHCCHHEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHEEECEEECCCCC
GALIVCSDQFYHSFLPRDLPFAQALADLTMIAIDEIQQRQRAAALQRDLVHTQLLRAEAE
CEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLNAAQAQLLQSAKLAAVGELAASVAHEINNPLYAARNSLYLVEQDLPPDAPQRTFLTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCCHHHHHH
AQQELGRIARIITRMRDFYRPSRGELSSTNVNALLRETLELVTTHLRHNHVAVHAHLAND
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
LPAITAHADQLRQVFLNVILNACDAMPNGGELTVKSSLIAARPEAPAALAIAISDTGVGI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECCCCCCEEEEEEEECCCCCC
APEHLPHLFEPFYTTKPHGTGLGLAISAHIVTQHGGRILVDSQPGSGTTFTILLPLEVPK
CHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCEEEEEEEECCCC
VE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]