Click here to switch to the map view.

The map label for this gene is epsC [H]

Identifier: 222524308

GI number: 222524308

Start: 1311454

End: 1313403

Strand: Reverse

Name: epsC [H]

Synonym: Chy400_1031

Alternate gene names: 222524308

Gene position: 1313403-1311454 (Counterclockwise)

Preceding gene: 222524309

Following gene: 222524307

Centisome position: 24.93

GC content: 53.9

Gene sequence:

>1950_bases
TTGAAGCTCATACCATCCCCTACTCGCAATCGTTATTTTTTCTTTCTCGACGCCATTCTGCTCCCATTAATGGCATACAT
GAGCTTCGTAGTACGGCTTGATGATCTTCCAAATGGCAATGCTCTGTTGGGATGGTTGATTCTTGCTATCATTGCCACCC
CAGTTCATCTCATCGTCTTTCGGCATCTGGGGGTTTACTCCCGCTACTGGCGTTATGCGTCGATTGACGAGCTGTTGCTG
CTCATTTCAGCTATTTCGCTGGCAATGCTGATCTCTACCCCAACTGCACTTGTTGTTGCTTCGGTAACACCCTTTGCTCT
TCTACCGCGCTCAGTTCCAATCATCTTCTTCTTCTTTGGCCTGGCAGCTACAATCGGTCCTCGTCTGATCGCGCGTATTC
GCTGGCATCGTGCAACCGTGAAGCGCAAGTCTAAAAGCGAATTGACCTTCAACATGCAGCGGGTGTTGATCATGGGGGCT
GGTTCAGCCGGCACGATGATTGCCCGTGAACTCCGCGATAATCCGCAACTCGGCATGGTTGCGGTCGGCTTTCTCGATGA
TGATCCCCTCAAGCAGGGCATGCATATCTACGGCGTGCCGGTGTTGGGTAATCGTTACGACATTCCGCGGCTGGCTCGTG
AGCGACAGGCACATTACGTCATTATTGCGATGCCTTCGGCGAGTGGTAAAGATATTCGCAGCATTGTTGAACTCTGTGAG
CGTACCAGAGTGAAGACCAAAATCATGCCTGGTCTTTACGAGATGCTTGATGGCAAGGTGAGCGTCAATCAGTTGCGGAA
TGTCCAGATTGAAGACTTGCTCCGCCGGCCTCCGGTACAGACAGATATTGCCGCTGTTCATCAGCTTCTGCGCGGGAAGC
GGGTGCTGGTCACCGGCGGTGGTGGCTCGATTGGCTCTGAGCTTTGTCGGCAAATCCTACGGGCCAGTCCTGAAGAGCTG
ATTATTCTCGGTCATGGCGAAAATTCGGTCTTTACTATCGAGCAGGAATTGCGTCGAGTCGCTCCGCCGACCACGAAGCT
TTCGGTGGTCATTGCTGACATCCGTTTCGCTGAACGCATCATGCACATCTTCGAGCAGTACCGGCCAGAGATAGTCTTTC
ACGCTGCGGCGCACAAGCACGTGCCGTTGATGGAGTTGCATCCCTCAGAAGCAGTGACGAATAACGTGCTCGGTACCCGC
AATCTGCTCAGCGCGACAATGCAGGTTGACGTAAGCCATTTTGTGATGATTTCCAGTGATAAAGCGGTTAATCCGACCAG
TGTGATGGGGGCGACAAAGCGTGTGGCTGAGCTGCTGGTACACGAGGCGGCCCGGCAGAGTGGGCGGGCGTATGTGGCTG
TGCGCTTTGGGAATGTGCTGGGTTCCCGCGGATCGGTTGTGCTGACCTTCAAGCAGCAGATCGCCGCCGGTGGCCCGGTA
ACGGTGACCCATCCTGAAATGCGCCGCTTCTTCATGACTATCCCTGAAGCGGTGCAATTAACGTTGCAGGCTTCGGTGTT
GGGGAAAGGTGGCGAGGTGTTTGTGCTCGATATGGGCGAACCAATCCGTATCGTCGATCTGGCTCGCGACATGATCGAGT
TGTCTGGCCTACAGGTTGGTCGCGATATTGATATCGTCTTTACCGGTCTGCGCCCCGGCGAGAAGCTCTATGAAGAGCTA
TTTGTTGAGGGTGAAGAATATGAACGGACGACCCACGCCAAGATCGTTATCGCTCGCAACGCATCACAGTTAGTACCGCG
CACACTGGCCGACCAAATTCGTATTCTCGAAATGGCAGCGCTCAACGATGATACAGCCGTACTGTTGCGTACCCTTCATC
GTTTGGTGCCAACCTTCAAACAGCCGACACCAATGCCGATGAACGAACCCAAACCACGCGAGCAGGCTGTTGGTGAGCCG
CTGTGGCGACGACAGTTAGCCAGTGATTAG

Upstream 100 bases:

>100_bases
TAAGCACAGCCACCACAAATATGTGCAGCGTTATCCAATTGCCAGCACCCCTCCCGCATACGCTGCCTTCCAGAAAGCGG
CAAACGGAAAGGAGCCTGTC

Downstream 100 bases:

>100_bases
GCAAAGGCATAGCGAAGTGTTCATGTGAGGGTGCAGTTATACACGTGTAGCGTTATCTGGAATGCGGAAGCCACGCTTCC
GCATTCCACTGTAAGGATGC

Product: polysaccharide biosynthesis protein CapD

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MKLIPSPTRNRYFFFLDAILLPLMAYMSFVVRLDDLPNGNALLGWLILAIIATPVHLIVFRHLGVYSRYWRYASIDELLL
LISAISLAMLISTPTALVVASVTPFALLPRSVPIIFFFFGLAATIGPRLIARIRWHRATVKRKSKSELTFNMQRVLIMGA
GSAGTMIARELRDNPQLGMVAVGFLDDDPLKQGMHIYGVPVLGNRYDIPRLARERQAHYVIIAMPSASGKDIRSIVELCE
RTRVKTKIMPGLYEMLDGKVSVNQLRNVQIEDLLRRPPVQTDIAAVHQLLRGKRVLVTGGGGSIGSELCRQILRASPEEL
IILGHGENSVFTIEQELRRVAPPTTKLSVVIADIRFAERIMHIFEQYRPEIVFHAAAHKHVPLMELHPSEAVTNNVLGTR
NLLSATMQVDVSHFVMISSDKAVNPTSVMGATKRVAELLVHEAARQSGRAYVAVRFGNVLGSRGSVVLTFKQQIAAGGPV
TVTHPEMRRFFMTIPEAVQLTLQASVLGKGGEVFVLDMGEPIRIVDLARDMIELSGLQVGRDIDIVFTGLRPGEKLYEEL
FVEGEEYERTTHAKIVIARNASQLVPRTLADQIRILEMAALNDDTAVLLRTLHRLVPTFKQPTPMPMNEPKPREQAVGEP
LWRRQLASD

Sequences:

>Translated_649_residues
MKLIPSPTRNRYFFFLDAILLPLMAYMSFVVRLDDLPNGNALLGWLILAIIATPVHLIVFRHLGVYSRYWRYASIDELLL
LISAISLAMLISTPTALVVASVTPFALLPRSVPIIFFFFGLAATIGPRLIARIRWHRATVKRKSKSELTFNMQRVLIMGA
GSAGTMIARELRDNPQLGMVAVGFLDDDPLKQGMHIYGVPVLGNRYDIPRLARERQAHYVIIAMPSASGKDIRSIVELCE
RTRVKTKIMPGLYEMLDGKVSVNQLRNVQIEDLLRRPPVQTDIAAVHQLLRGKRVLVTGGGGSIGSELCRQILRASPEEL
IILGHGENSVFTIEQELRRVAPPTTKLSVVIADIRFAERIMHIFEQYRPEIVFHAAAHKHVPLMELHPSEAVTNNVLGTR
NLLSATMQVDVSHFVMISSDKAVNPTSVMGATKRVAELLVHEAARQSGRAYVAVRFGNVLGSRGSVVLTFKQQIAAGGPV
TVTHPEMRRFFMTIPEAVQLTLQASVLGKGGEVFVLDMGEPIRIVDLARDMIELSGLQVGRDIDIVFTGLRPGEKLYEEL
FVEGEEYERTTHAKIVIARNASQLVPRTLADQIRILEMAALNDDTAVLLRTLHRLVPTFKQPTPMPMNEPKPREQAVGEP
LWRRQLASD
>Mature_649_residues
MKLIPSPTRNRYFFFLDAILLPLMAYMSFVVRLDDLPNGNALLGWLILAIIATPVHLIVFRHLGVYSRYWRYASIDELLL
LISAISLAMLISTPTALVVASVTPFALLPRSVPIIFFFFGLAATIGPRLIARIRWHRATVKRKSKSELTFNMQRVLIMGA
GSAGTMIARELRDNPQLGMVAVGFLDDDPLKQGMHIYGVPVLGNRYDIPRLARERQAHYVIIAMPSASGKDIRSIVELCE
RTRVKTKIMPGLYEMLDGKVSVNQLRNVQIEDLLRRPPVQTDIAAVHQLLRGKRVLVTGGGGSIGSELCRQILRASPEEL
IILGHGENSVFTIEQELRRVAPPTTKLSVVIADIRFAERIMHIFEQYRPEIVFHAAAHKHVPLMELHPSEAVTNNVLGTR
NLLSATMQVDVSHFVMISSDKAVNPTSVMGATKRVAELLVHEAARQSGRAYVAVRFGNVLGSRGSVVLTFKQQIAAGGPV
TVTHPEMRRFFMTIPEAVQLTLQASVLGKGGEVFVLDMGEPIRIVDLARDMIELSGLQVGRDIDIVFTGLRPGEKLYEEL
FVEGEEYERTTHAKIVIARNASQLVPRTLADQIRILEMAALNDDTAVLLRTLHRLVPTFKQPTPMPMNEPKPREQAVGEP
LWRRQLASD

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=261, Percent_Identity=25.2873563218391, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 72320; Mature: 72320

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIPSPTRNRYFFFLDAILLPLMAYMSFVVRLDDLPNGNALLGWLILAIIATPVHLIVF
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RHLGVYSRYWRYASIDELLLLISAISLAMLISTPTALVVASVTPFALLPRSVPIIFFFFG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHCCCCHHHHHHHHH
LAATIGPRLIARIRWHRATVKRKSKSELTFNMQRVLIMGAGSAGTMIARELRDNPQLGMV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEE
AVGFLDDDPLKQGMHIYGVPVLGNRYDIPRLARERQAHYVIIAMPSASGKDIRSIVELCE
EEEECCCCHHHCCCEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
RTRVKTKIMPGLYEMLDGKVSVNQLRNVQIEDLLRRPPVQTDIAAVHQLLRGKRVLVTGG
HHHHHHHHHHHHHHHHCCCEEHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCEEEEECC
GGSIGSELCRQILRASPEELIILGHGENSVFTIEQELRRVAPPTTKLSVVIADIRFAERI
CCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHCCCCCEEEEEEEHHHHHHHH
MHIFEQYRPEIVFHAAAHKHVPLMELHPSEAVTNNVLGTRNLLSATMQVDVSHFVMISSD
HHHHHHHCCCEEEEECCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHEECEEEEEEEECC
KAVNPTSVMGATKRVAELLVHEAARQSGRAYVAVRFGNVLGSRGSVVLTFKQQIAAGGPV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHCCCCCEEEEEEHHHCCCCCE
TVTHPEMRRFFMTIPEAVQLTLQASVLGKGGEVFVLDMGEPIRIVDLARDMIELSGLQVG
EECCHHHHHHHHHCCHHHHEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
RDIDIVFTGLRPGEKLYEELFVEGEEYERTTHAKIVIARNASQLVPRTLADQIRILEMAA
CCEEEEEECCCCHHHHHHHHCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
LNDDTAVLLRTLHRLVPTFKQPTPMPMNEPKPREQAVGEPLWRRQLASD
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MKLIPSPTRNRYFFFLDAILLPLMAYMSFVVRLDDLPNGNALLGWLILAIIATPVHLIVF
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RHLGVYSRYWRYASIDELLLLISAISLAMLISTPTALVVASVTPFALLPRSVPIIFFFFG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHCCCCHHHHHHHHH
LAATIGPRLIARIRWHRATVKRKSKSELTFNMQRVLIMGAGSAGTMIARELRDNPQLGMV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEE
AVGFLDDDPLKQGMHIYGVPVLGNRYDIPRLARERQAHYVIIAMPSASGKDIRSIVELCE
EEEECCCCHHHCCCEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
RTRVKTKIMPGLYEMLDGKVSVNQLRNVQIEDLLRRPPVQTDIAAVHQLLRGKRVLVTGG
HHHHHHHHHHHHHHHHCCCEEHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCEEEEECC
GGSIGSELCRQILRASPEELIILGHGENSVFTIEQELRRVAPPTTKLSVVIADIRFAERI
CCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHCCCCCEEEEEEEHHHHHHHH
MHIFEQYRPEIVFHAAAHKHVPLMELHPSEAVTNNVLGTRNLLSATMQVDVSHFVMISSD
HHHHHHHCCCEEEEECCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHEECEEEEEEEECC
KAVNPTSVMGATKRVAELLVHEAARQSGRAYVAVRFGNVLGSRGSVVLTFKQQIAAGGPV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHCCCCCEEEEEEHHHCCCCCE
TVTHPEMRRFFMTIPEAVQLTLQASVLGKGGEVFVLDMGEPIRIVDLARDMIELSGLQVG
EECCHHHHHHHHHCCHHHHEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCC
RDIDIVFTGLRPGEKLYEELFVEGEEYERTTHAKIVIARNASQLVPRTLADQIRILEMAA
CCEEEEEECCCCHHHHHHHHCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
LNDDTAVLLRTLHRLVPTFKQPTPMPMNEPKPREQAVGEPLWRRQLASD
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]