Click here to switch to the map view.

The map label for this gene is amiD [H]

Identifier: 222524241

GI number: 222524241

Start: 1229252

End: 1231156

Strand: Direct

Name: amiD [H]

Synonym: Chy400_0963

Alternate gene names: 222524241

Gene position: 1229252-1231156 (Clockwise)

Preceding gene: 222524240

Following gene: 222524242

Centisome position: 23.33

GC content: 60.21

Gene sequence:

>1905_bases
ATGTATCAGATTGAATGGCTAGGAATGACGCGCAACAATTTCAAGCCCGGCACCCCTGATCGCATCCGTATGGTGGTGTT
ACACGCCACCGCCGGCACCTATCCCGGTGATCTGAAGTGGCTGCGTCAGGGTGGTGCGCCAGGCCGTGAAGTGTCTGTGC
ATTACTACATCAATAAACGTGGACACATTGTGCAACTGGTTGCCGATCACGATATTGCCTGGCACGCCGGTGTCAGTCGT
TGGGAGGTGGACGGACGTACCGTCTACGGCTGTAACGATGTGTCGATTGGGATTGAGTTAGAGAACCGGAACGATGGTCG
CGATCCGTATCCGCCGGAACAGTATGCGGCGGCACGCTGGTTAACCCGCGAGCTGGTGCAGAAGTATCAGATTCCGCCAA
ACCAGGTTGTCCGCCACCTTGACATTGCCCCCGGTCGGAAGACCGATCCGGCTGGCTTCCCCTGGCAACGCTTTGTCAGT
GAGGTGTTTGCCGATCTTCCCGGCGCCCCGACGATGCCGCCGACCGAGCAGATGCGCCAACGGATGCTCGATGTCGCCTA
TCGCGCCGCCGGGAGTGGCCTGCCCGCGAGCTGGCCCTTCGCTACCACAGCACGTACCCTTCATCTGGGGATGCCGGTGG
CCTCGTTAATCGCCCGTCCACCGGCATCACGTGCGGCATCATCACCGAATGATCGGGAACGAGTGTTGTCACTTCCCGAT
GGCACCCGCTATCTGGTGGAGGTCTACGCTCGTGATGCGCTCTTTGCAACCGTTGGCCCGGATGACACGCTGCGCCCCGA
TGAGCCAATTCAGCGATTACGTGATGTTCCCGCCAGCCCGCAACGTCTGGCGCTCCTCGACGCTATCTTCCGGGCTGCCG
ACCCGATCAACGGGTTTCAACCGGGCTGGGCATTCCATCAGTATTTTCTCGACCATGAGGCTGAACTTGGCATGCCAATC
AGTCACAACCACCGCCTCGCTCTGGCACCGGGATGGCATGTCGCCTGCCAGCACTTCGCCCTCGACAGCCTCTGCTCGCC
GGTTGGTCAGTGGCAGGTAATCTACCGTCTGAGTGATATTCGACGTGCTGCGGCAGGCCAGATGATTCTTCCCGGTCTGA
GTCGTGAGCGAGCCAGGCAACTGGCGAATCTCCTGCTTGATGATCTCTTTGCCCTGCGTACCGGTCGGCGGTATCAGGCT
GAGGCGGCGCTCGTGCGGTACGCGCTTGACGAAGATCTGGGCGCACCGCTTGGCCCACCCGAAACAGATGTCGTTGATGG
CATCCCCATCGCGTTGATGCCGTTTGCCCTCGATGTGGTGGCCTGTCGCCTGCCGGCACCGGACTGGCCACTCGACCGGC
CACTGCCACCGGGCAATCCGTTCGGTCGTCTCACCGCATTGCTCAGCTCACACCGTCAGGGCGTATCCGGTGTTATCCGA
TTATCACGGATGCACAATCCGCGTTCGCTCCCGGTGATGCAGGCAACCCCGTTGGTGGGATCACCCCGACCTGATCGCCC
GTTCATCGATGTCAGTCTCTTTGCCGGTGACGGTGAGGTACGTCGGCGAGCGATTGATACCATTGTCGTTATCCCAACGC
CAGGACCGGCGAGCCTGGCACTGACGCAGGCGCATACGGCTGCGCGCTGGCACTACTACATCGATCATACCGGCACCGTC
TTTCGGTTGCGGCACGAACGCTATGTTGCGCGGGCTGCCCGTGGTATCGGCCTGCCTGCCGAACAGCTCGATCAACGGGC
ACTGGTCATTGCTGTTGAGGGTGAACTTGACCACGCCCCGCCGACACTTCGTAAAGCAGCAAAGGCGCTCATTCAGTTAC
TCTGCCGACATTTTCAGATTAGCAGCAACCGGATACACGTAGGCGAAATGACCATGCATTTGTAG

Upstream 100 bases:

>100_bases
CGGCGTTTACGCATTCAAGAGGAATGGGCACGTCAGCGTTTGCAAGCAGATCGGCAGTAAGGGAATCTGCCGGCGCTGTT
GAACATATATCAGGTGAGTC

Downstream 100 bases:

>100_bases
GCGGCAATCGGTTCGCTGCCACCTATCACAACATCACAGGAATCATATGCAGTCAACAGCTTCTTCGTTTCACGCCCGCG
CCCCGATCAACATCAAGCTG

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MYQIEWLGMTRNNFKPGTPDRIRMVVLHATAGTYPGDLKWLRQGGAPGREVSVHYYINKRGHIVQLVADHDIAWHAGVSR
WEVDGRTVYGCNDVSIGIELENRNDGRDPYPPEQYAAARWLTRELVQKYQIPPNQVVRHLDIAPGRKTDPAGFPWQRFVS
EVFADLPGAPTMPPTEQMRQRMLDVAYRAAGSGLPASWPFATTARTLHLGMPVASLIARPPASRAASSPNDRERVLSLPD
GTRYLVEVYARDALFATVGPDDTLRPDEPIQRLRDVPASPQRLALLDAIFRAADPINGFQPGWAFHQYFLDHEAELGMPI
SHNHRLALAPGWHVACQHFALDSLCSPVGQWQVIYRLSDIRRAAAGQMILPGLSRERARQLANLLLDDLFALRTGRRYQA
EAALVRYALDEDLGAPLGPPETDVVDGIPIALMPFALDVVACRLPAPDWPLDRPLPPGNPFGRLTALLSSHRQGVSGVIR
LSRMHNPRSLPVMQATPLVGSPRPDRPFIDVSLFAGDGEVRRRAIDTIVVIPTPGPASLALTQAHTAARWHYYIDHTGTV
FRLRHERYVARAARGIGLPAEQLDQRALVIAVEGELDHAPPTLRKAAKALIQLLCRHFQISSNRIHVGEMTMHL

Sequences:

>Translated_634_residues
MYQIEWLGMTRNNFKPGTPDRIRMVVLHATAGTYPGDLKWLRQGGAPGREVSVHYYINKRGHIVQLVADHDIAWHAGVSR
WEVDGRTVYGCNDVSIGIELENRNDGRDPYPPEQYAAARWLTRELVQKYQIPPNQVVRHLDIAPGRKTDPAGFPWQRFVS
EVFADLPGAPTMPPTEQMRQRMLDVAYRAAGSGLPASWPFATTARTLHLGMPVASLIARPPASRAASSPNDRERVLSLPD
GTRYLVEVYARDALFATVGPDDTLRPDEPIQRLRDVPASPQRLALLDAIFRAADPINGFQPGWAFHQYFLDHEAELGMPI
SHNHRLALAPGWHVACQHFALDSLCSPVGQWQVIYRLSDIRRAAAGQMILPGLSRERARQLANLLLDDLFALRTGRRYQA
EAALVRYALDEDLGAPLGPPETDVVDGIPIALMPFALDVVACRLPAPDWPLDRPLPPGNPFGRLTALLSSHRQGVSGVIR
LSRMHNPRSLPVMQATPLVGSPRPDRPFIDVSLFAGDGEVRRRAIDTIVVIPTPGPASLALTQAHTAARWHYYIDHTGTV
FRLRHERYVARAARGIGLPAEQLDQRALVIAVEGELDHAPPTLRKAAKALIQLLCRHFQISSNRIHVGEMTMHL
>Mature_634_residues
MYQIEWLGMTRNNFKPGTPDRIRMVVLHATAGTYPGDLKWLRQGGAPGREVSVHYYINKRGHIVQLVADHDIAWHAGVSR
WEVDGRTVYGCNDVSIGIELENRNDGRDPYPPEQYAAARWLTRELVQKYQIPPNQVVRHLDIAPGRKTDPAGFPWQRFVS
EVFADLPGAPTMPPTEQMRQRMLDVAYRAAGSGLPASWPFATTARTLHLGMPVASLIARPPASRAASSPNDRERVLSLPD
GTRYLVEVYARDALFATVGPDDTLRPDEPIQRLRDVPASPQRLALLDAIFRAADPINGFQPGWAFHQYFLDHEAELGMPI
SHNHRLALAPGWHVACQHFALDSLCSPVGQWQVIYRLSDIRRAAAGQMILPGLSRERARQLANLLLDDLFALRTGRRYQA
EAALVRYALDEDLGAPLGPPETDVVDGIPIALMPFALDVVACRLPAPDWPLDRPLPPGNPFGRLTALLSSHRQGVSGVIR
LSRMHNPRSLPVMQATPLVGSPRPDRPFIDVSLFAGDGEVRRRAIDTIVVIPTPGPASLALTQAHTAARWHYYIDHTGTV
FRLRHERYVARAARGIGLPAEQLDQRALVIAVEGELDHAPPTLRKAAKALIQLLCRHFQISSNRIHVGEMTMHL

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=183, Percent_Identity=36.0655737704918, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1786300, Length=109, Percent_Identity=44.0366972477064, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 70481; Mature: 70481

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQIEWLGMTRNNFKPGTPDRIRMVVLHATAGTYPGDLKWLRQGGAPGREVSVHYYINKR
CEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCEEEEEEEECCC
GHIVQLVADHDIAWHAGVSRWEVDGRTVYGCNDVSIGIELENRNDGRDPYPPEQYAAARW
CCEEEEEECCCEEECCCCCEEEECCEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LTRELVQKYQIPPNQVVRHLDIAPGRKTDPAGFPWQRFVSEVFADLPGAPTMPPTEQMRQ
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
RMLDVAYRAAGSGLPASWPFATTARTLHLGMPVASLIARPPASRAASSPNDRERVLSLPD
HHHHHHHHHCCCCCCCCCCCHHCHHEEEECCCHHHHHCCCCHHHCCCCCCCHHHEEECCC
GTRYLVEVYARDALFATVGPDDTLRPDEPIQRLRDVPASPQRLALLDAIFRAADPINGFQ
CHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
PGWAFHQYFLDHEAELGMPISHNHRLALAPGWHVACQHFALDSLCSPVGQWQVIYRLSDI
CCHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHH
RRAAAGQMILPGLSRERARQLANLLLDDLFALRTGRRYQAEAALVRYALDEDLGAPLGPP
HHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
ETDVVDGIPIALMPFALDVVACRLPAPDWPLDRPLPPGNPFGRLTALLSSHRQGVSGVIR
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
LSRMHNPRSLPVMQATPLVGSPRPDRPFIDVSLFAGDGEVRRRAIDTIVVIPTPGPASLA
HHHCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEECCHHHHHEEECEEEEEECCCCCCEE
LTQAHTAARWHYYIDHTGTVFRLRHERYVARAARGIGLPAEQLDQRALVIAVEGELDHAP
EEHHHHCEEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCHHHHCCCEEEEEEECCCCCCC
PTLRKAAKALIQLLCRHFQISSNRIHVGEMTMHL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECC
>Mature Secondary Structure
MYQIEWLGMTRNNFKPGTPDRIRMVVLHATAGTYPGDLKWLRQGGAPGREVSVHYYINKR
CEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCEEEEEEEECCC
GHIVQLVADHDIAWHAGVSRWEVDGRTVYGCNDVSIGIELENRNDGRDPYPPEQYAAARW
CCEEEEEECCCEEECCCCCEEEECCEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LTRELVQKYQIPPNQVVRHLDIAPGRKTDPAGFPWQRFVSEVFADLPGAPTMPPTEQMRQ
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
RMLDVAYRAAGSGLPASWPFATTARTLHLGMPVASLIARPPASRAASSPNDRERVLSLPD
HHHHHHHHHCCCCCCCCCCCHHCHHEEEECCCHHHHHCCCCHHHCCCCCCCHHHEEECCC
GTRYLVEVYARDALFATVGPDDTLRPDEPIQRLRDVPASPQRLALLDAIFRAADPINGFQ
CHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
PGWAFHQYFLDHEAELGMPISHNHRLALAPGWHVACQHFALDSLCSPVGQWQVIYRLSDI
CCHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHH
RRAAAGQMILPGLSRERARQLANLLLDDLFALRTGRRYQAEAALVRYALDEDLGAPLGPP
HHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
ETDVVDGIPIALMPFALDVVACRLPAPDWPLDRPLPPGNPFGRLTALLSSHRQGVSGVIR
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
LSRMHNPRSLPVMQATPLVGSPRPDRPFIDVSLFAGDGEVRRRAIDTIVVIPTPGPASLA
HHHCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEECCHHHHHEEECEEEEEECCCCCCEE
LTQAHTAARWHYYIDHTGTVFRLRHERYVARAARGIGLPAEQLDQRALVIAVEGELDHAP
EEHHHHCEEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCHHHHCCCEEEEEEECCCCCCC
PTLRKAAKALIQLLCRHFQISSNRIHVGEMTMHL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]