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The map label for this gene is fruA [H]

Identifier: 222524153

GI number: 222524153

Start: 1099912

End: 1101354

Strand: Direct

Name: fruA [H]

Synonym: Chy400_0869

Alternate gene names: 222524153

Gene position: 1099912-1101354 (Clockwise)

Preceding gene: 222524152

Following gene: 222524155

Centisome position: 20.88

GC content: 61.68

Gene sequence:

>1443_bases
ATGGCGGTCATTGTCGCAATTACGTCATGTCCCACCGGTATCGCGCATACCTTTATGGCAGCGGAGGGGCTACAACGGGG
AGCAGAGAGTCTCGGCCACACGATCAAGGTCGAGACACAGGGGTCGGTTGGCGCCGAAAACACGCTGACGGCGGCTGATA
TTGCCGCTGCCGAGGTGGTGATCATTGCCGCCGACACCAAAGTCGATCTGAGTCGGTTTGCCGGCAAGCGGATTTACGAA
ACCAGCACGAAAGCGGCAATTCACGACGGGGCAGGCGTGGTGAAGGCAGCACTTGCCCAGGCCACCACCAAAGCAGTATC
GGCGGGGGCACGATCCGATCTGGTTGATCAGGTCGCAGCGGCAAAGGCGGCCCGCGCATCAGGCGTAGCCGGCCCGTACA
AGCATCTGATGACCGGCGTCAGCTATATGCTCCCCTTCGTGGTAGCCGGTGGTCTGCTCATTGCCCTGGCATTTGCCTTC
GGCGGTATCTACGTCTACGAAGATGCAAACCGTGGCACGCTGGGCTGGGCATTGTTCCAGATCGGTGCCCCATCGGCCTT
CGCGCTGATGGTACCCATCCTCGCCGGCTTCATCGCCTTCTCTATCGCAGATCGGCCCGGTCTGGCCCCCGGTATGATCG
GTGGCATGCTGGCCTCTTCCACCGGTTCCGGCTTCCTCGGCGGTATCCTGGCCGGGTTCATCGCCGGTTACGCGACGCAA
TGGCTCAACCAGAACCTCAACCTGCCCAAGACACTGGCCGGCCTGAAGCCGGTACTGATCCTGCCGCTGCTCAGCTCGCT
ATTCGTCGGTCTGCTGATGGTCTACGTGATCGGCCAGCCGGTGAGTTCAGCCCTCAATGCCTTAACGGCCTGGCTGCAAA
GCATGGAGCAGAGCAGTGCGCTAATCCTCGGCCTGATCCTGGGTGCGATGATGGCCTTCGATATGGGTGGCCCGGTCAAC
AAAGCTGCCTACACCTTCGCCACCGGACTGCTGGCCTCGCAAGTCACGACGCCGATGGCAGCCGTGATGGCCGCCGGTAT
GACCCCACCGCTCGGCCTGGCACTGGCGACCCTGCTGTTCAAAGATCGCTTCTCTGACGAAGAGCGTGAGGCGGGCAAGG
CCGCTGCCGTACTCGGCATCTCCTTCATCACCGAAGGTGCCATTCCCTTCGCGGCCAAAGACCCCTTCCGCGTCATTCCT
TCGATTATGGTTGGGTCGGCAGTAGCCGGTGCGCTCTCGATGCTCTTCGGGGCAACGCTGGCTGTGCCACACGGTGGTAT
CTTCGTGCTGCCTATTCCGAATGCTGTCGGCAACCTGCCGATGTACATCGTCGCCATCCTGGCCGGTACCCTGGTAACCG
CAGCGATGCTCTATGTGCTCAAGCGACCACTGAATACCGCCGAAGCACCGACAACGGCAGCAGTGCGGGGGACGGCTGAT
TAG

Upstream 100 bases:

>100_bases
CGCTGGCAGCAAAAGTTACCGTTACCGTGGTTGATCCGGCTGTCACGACCGTGACGCCTGTTGATCAATAGAAGTTGTAA
GGAGCAAAGGGAGGAGAACC

Downstream 100 bases:

>100_bases
TCATCGCAACCAGCGTTACACACGCAACTCAATCGTTGCAGCAACCGGGGTGCGGAAGCCAGGCTTCCGCACCCCAAGTG
TGACTACTGAATTCATCCTC

Product: PTS system, fructose subfamily, IIC subunit

Products: NA

Alternate protein names: EIIBBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAVIVAITSCPTGIAHTFMAAEGLQRGAESLGHTIKVETQGSVGAENTLTAADIAAAEVVIIAADTKVDLSRFAGKRIYE
TSTKAAIHDGAGVVKAALAQATTKAVSAGARSDLVDQVAAAKAARASGVAGPYKHLMTGVSYMLPFVVAGGLLIALAFAF
GGIYVYEDANRGTLGWALFQIGAPSAFALMVPILAGFIAFSIADRPGLAPGMIGGMLASSTGSGFLGGILAGFIAGYATQ
WLNQNLNLPKTLAGLKPVLILPLLSSLFVGLLMVYVIGQPVSSALNALTAWLQSMEQSSALILGLILGAMMAFDMGGPVN
KAAYTFATGLLASQVTTPMAAVMAAGMTPPLGLALATLLFKDRFSDEEREAGKAAAVLGISFITEGAIPFAAKDPFRVIP
SIMVGSAVAGALSMLFGATLAVPHGGIFVLPIPNAVGNLPMYIVAILAGTLVTAAMLYVLKRPLNTAEAPTTAAVRGTAD

Sequences:

>Translated_480_residues
MAVIVAITSCPTGIAHTFMAAEGLQRGAESLGHTIKVETQGSVGAENTLTAADIAAAEVVIIAADTKVDLSRFAGKRIYE
TSTKAAIHDGAGVVKAALAQATTKAVSAGARSDLVDQVAAAKAARASGVAGPYKHLMTGVSYMLPFVVAGGLLIALAFAF
GGIYVYEDANRGTLGWALFQIGAPSAFALMVPILAGFIAFSIADRPGLAPGMIGGMLASSTGSGFLGGILAGFIAGYATQ
WLNQNLNLPKTLAGLKPVLILPLLSSLFVGLLMVYVIGQPVSSALNALTAWLQSMEQSSALILGLILGAMMAFDMGGPVN
KAAYTFATGLLASQVTTPMAAVMAAGMTPPLGLALATLLFKDRFSDEEREAGKAAAVLGISFITEGAIPFAAKDPFRVIP
SIMVGSAVAGALSMLFGATLAVPHGGIFVLPIPNAVGNLPMYIVAILAGTLVTAAMLYVLKRPLNTAEAPTTAAVRGTAD
>Mature_479_residues
AVIVAITSCPTGIAHTFMAAEGLQRGAESLGHTIKVETQGSVGAENTLTAADIAAAEVVIIAADTKVDLSRFAGKRIYET
STKAAIHDGAGVVKAALAQATTKAVSAGARSDLVDQVAAAKAARASGVAGPYKHLMTGVSYMLPFVVAGGLLIALAFAFG
GIYVYEDANRGTLGWALFQIGAPSAFALMVPILAGFIAFSIADRPGLAPGMIGGMLASSTGSGFLGGILAGFIAGYATQW
LNQNLNLPKTLAGLKPVLILPLLSSLFVGLLMVYVIGQPVSSALNALTAWLQSMEQSSALILGLILGAMMAFDMGGPVNK
AAYTFATGLLASQVTTPMAAVMAAGMTPPLGLALATLLFKDRFSDEEREAGKAAAVLGISFITEGAIPFAAKDPFRVIPS
IMVGSAVAGALSMLFGATLAVPHGGIFVLPIPNAVGNLPMYIVAILAGTLVTAAMLYVLKRPLNTAEAPTTAAVRGTAD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=460, Percent_Identity=56.9565217391304, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1786951, Length=467, Percent_Identity=37.4732334047109, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI87082348, Length=440, Percent_Identity=37.7272727272727, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1790386, Length=300, Percent_Identity=46.6666666666667, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788729, Length=377, Percent_Identity=31.5649867374005, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1790387, Length=104, Percent_Identity=45.1923076923077, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788730, Length=93, Percent_Identity=44.0860215053763, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 48755; Mature: 48624

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIVAITSCPTGIAHTFMAAEGLQRGAESLGHTIKVETQGSVGAENTLTAADIAAAEVV
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHEEEE
IIAADTKVDLSRFAGKRIYETSTKAAIHDGAGVVKAALAQATTKAVSAGARSDLVDQVAA
EEEECCCHHHHHHCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AKAARASGVAGPYKHLMTGVSYMLPFVVAGGLLIALAFAFGGIYVYEDANRGTLGWALFQ
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHH
IGAPSAFALMVPILAGFIAFSIADRPGLAPGMIGGMLASSTGSGFLGGILAGFIAGYATQ
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
WLNQNLNLPKTLAGLKPVLILPLLSSLFVGLLMVYVIGQPVSSALNALTAWLQSMEQSSA
HHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LILGLILGAMMAFDMGGPVNKAAYTFATGLLASQVTTPMAAVMAAGMTPPLGLALATLLF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KDRFSDEEREAGKAAAVLGISFITEGAIPFAAKDPFRVIPSIMVGSAVAGALSMLFGATL
HHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVPHGGIFVLPIPNAVGNLPMYIVAILAGTLVTAAMLYVLKRPLNTAEAPTTAAVRGTAD
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVIVAITSCPTGIAHTFMAAEGLQRGAESLGHTIKVETQGSVGAENTLTAADIAAAEVV
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHEEEE
IIAADTKVDLSRFAGKRIYETSTKAAIHDGAGVVKAALAQATTKAVSAGARSDLVDQVAA
EEEECCCHHHHHHCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AKAARASGVAGPYKHLMTGVSYMLPFVVAGGLLIALAFAFGGIYVYEDANRGTLGWALFQ
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHH
IGAPSAFALMVPILAGFIAFSIADRPGLAPGMIGGMLASSTGSGFLGGILAGFIAGYATQ
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
WLNQNLNLPKTLAGLKPVLILPLLSSLFVGLLMVYVIGQPVSSALNALTAWLQSMEQSSA
HHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LILGLILGAMMAFDMGGPVNKAAYTFATGLLASQVTTPMAAVMAAGMTPPLGLALATLLF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KDRFSDEEREAGKAAAVLGISFITEGAIPFAAKDPFRVIPSIMVGSAVAGALSMLFGATL
HHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVPHGGIFVLPIPNAVGNLPMYIVAILAGTLVTAAMLYVLKRPLNTAEAPTTAAVRGTAD
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254279 [H]