Click here to switch to the map view.

The map label for this gene is fixJ [H]

Identifier: 222524071

GI number: 222524071

Start: 994858

End: 995229

Strand: Direct

Name: fixJ [H]

Synonym: Chy400_0787

Alternate gene names: 222524071

Gene position: 994858-995229 (Clockwise)

Preceding gene: 222524069

Following gene: 222524074

Centisome position: 18.88

GC content: 52.15

Gene sequence:

>372_bases
ATGCCAGCGAATAAGGGTACTATCTTTATTATCGACGACGACCTGGGGCTCCAAAACATTCTCTCCATTGCCCTGCGCAA
CGCCGGTTACGATGTTGTGCTGGCCCGCGACGGCCTCGAAGGTCTGCGCATGCTCGAGCACCTCTCACCCAGTCTGGTTA
TCAGCGACATTATGATGCCGAACATGGACGGCGTTGAGACCTTCCAGCGTATTAAGGAACGTCTGCAAGATAATGGCATT
CCGATCTTCTTGATCACTGCGCTCAACCGCAAGCCATGGTTTGCCGATCTTGAGGCTGAAGGCGCAGTGATTATTCAGAA
GCCGTTTGAGGTACATCATCTGGTTGAGCTGATTGATCGGACACTGAGCTAA

Upstream 100 bases:

>100_bases
ACCCACTGTCTACTCTCTGGTATAATGGCGCTCAACGCGACGCCAATCCACTCTTGTATTATCCCGGCGCAGTTCATTCA
GAACCGGTAAGGACATCGAT

Downstream 100 bases:

>100_bases
GTGCAGCAAAGTGTGGCCGGAGTCGGGCGTAGGTCTCGTCGAGCGCTTCTGGGATCAGTTTTGTATCGCCAATCACCGGC
ATAAAGTTGGTATCGCCATT

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MPANKGTIFIIDDDLGLQNILSIALRNAGYDVVLARDGLEGLRMLEHLSPSLVISDIMMPNMDGVETFQRIKERLQDNGI
PIFLITALNRKPWFADLEAEGAVIIQKPFEVHHLVELIDRTLS

Sequences:

>Translated_123_residues
MPANKGTIFIIDDDLGLQNILSIALRNAGYDVVLARDGLEGLRMLEHLSPSLVISDIMMPNMDGVETFQRIKERLQDNGI
PIFLITALNRKPWFADLEAEGAVIIQKPFEVHHLVELIDRTLS
>Mature_122_residues
PANKGTIFIIDDDLGLQNILSIALRNAGYDVVLARDGLEGLRMLEHLSPSLVISDIMMPNMDGVETFQRIKERLQDNGIP
IFLITALNRKPWFADLEAEGAVIIQKPFEVHHLVELIDRTLS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13807; Mature: 13676

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANKGTIFIIDDDLGLQNILSIALRNAGYDVVLARDGLEGLRMLEHLSPSLVISDIMMP
CCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCC
NMDGVETFQRIKERLQDNGIPIFLITALNRKPWFADLEAEGAVIIQKPFEVHHLVELIDR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCEEEEECCHHHHHHHHHHHH
TLS
HCC
>Mature Secondary Structure 
PANKGTIFIIDDDLGLQNILSIALRNAGYDVVLARDGLEGLRMLEHLSPSLVISDIMMP
CCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCC
NMDGVETFQRIKERLQDNGIPIFLITALNRKPWFADLEAEGAVIIQKPFEVHHLVELIDR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCEEEEECCHHHHHHHHHHHH
TLS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]