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The map label for this gene is bioH [H]

Identifier: 222524011

GI number: 222524011

Start: 907633

End: 908454

Strand: Direct

Name: bioH [H]

Synonym: Chy400_0726

Alternate gene names: 222524011

Gene position: 907633-908454 (Clockwise)

Preceding gene: 222524003

Following gene: 222524012

Centisome position: 17.23

GC content: 59.37

Gene sequence:

>822_bases
ATGTCAATCGTCCAAATCATGCAGCACTACCACCACCGGCTTGGGATCAGCTATCTGACTGCCGGTGAGTCTGGCCCGGC
CATCGTGTTTCTCCACGGGTGGGGCGCATTTAAGGAACTCTGGTGGAGCGCCCTTCGCGACCTTGGCCGTGATTACCGCT
GTTTTGCCATCGATATGCCCGGTCATGGCGAGAGTCGGATTGGACGGGCCGACCAGATTGAGCGGATTGCCGTGTTGATT
GCCGATTTTTGCCATGATCTCGGCCTTTCAACCATCATTCTGGTCGGTCATTCGATGGGGGGCAGCGTTGCCGTCGAGAT
GACTCTTCACTACCCCCACCTGGTACAGCGCCTGGCTCTTATTGATGCGGCAGTCGACGCCTATCGCATGCCGGCCTATA
CCCGTATCTATCTGTTGCCACATCTCGGCTGGCCAACCTTTCGCCTGACCCAGGCTATTGGACGTGCGTTTCGACCGCTC
GGTCAGCGGATACCGCACGAGCACGGTGGCGGCTGGATTCGTCCGTGGTTACGACGGGCATCGTACCTCGCCACGTTTGA
CCCAGAAGGATTGTACCGCATTTTGCGTTCGCTCTTCGCGACTCGCGCAGACGAACGACTGCAACAGATCCGTGTCCCAA
CCCTGGTAATGACCGGGCAGTTCGATAGTCTGGCCCCGCCTGCCCACGCTCGCCGTCTGGCCCAGGTGATACCCGGTGCA
CGCTATGTCATGATTCCGGTCAGTCTCCACAACCCAATGGACGAACGACCGCGGGCATTCACCCGCGCTCTCCGCGCATT
TCTCGCCGAGACCGACTCGTAA

Upstream 100 bases:

>100_bases
TACCTGCTCTAGCGATAGCTGCCGTTTCGATTGTGGTGAAAAGGTATAATAGTGCCACAATCGGGCAAGACTGTTCTGAA
ATCCGTTCAGTAGCGATAGT

Downstream 100 bases:

>100_bases
GCGGCAAGATAATCCAAGTTGCCACCTTACGTCACTACAGCGGTGCGGGTCGTTGAGCGCGACGGGGGAGGGTGGAACAC
GGATGGACGCGGGTGAGGCG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSIVQIMQHYHHRLGISYLTAGESGPAIVFLHGWGAFKELWWSALRDLGRDYRCFAIDMPGHGESRIGRADQIERIAVLI
ADFCHDLGLSTIILVGHSMGGSVAVEMTLHYPHLVQRLALIDAAVDAYRMPAYTRIYLLPHLGWPTFRLTQAIGRAFRPL
GQRIPHEHGGGWIRPWLRRASYLATFDPEGLYRILRSLFATRADERLQQIRVPTLVMTGQFDSLAPPAHARRLAQVIPGA
RYVMIPVSLHNPMDERPRAFTRALRAFLAETDS

Sequences:

>Translated_273_residues
MSIVQIMQHYHHRLGISYLTAGESGPAIVFLHGWGAFKELWWSALRDLGRDYRCFAIDMPGHGESRIGRADQIERIAVLI
ADFCHDLGLSTIILVGHSMGGSVAVEMTLHYPHLVQRLALIDAAVDAYRMPAYTRIYLLPHLGWPTFRLTQAIGRAFRPL
GQRIPHEHGGGWIRPWLRRASYLATFDPEGLYRILRSLFATRADERLQQIRVPTLVMTGQFDSLAPPAHARRLAQVIPGA
RYVMIPVSLHNPMDERPRAFTRALRAFLAETDS
>Mature_272_residues
SIVQIMQHYHHRLGISYLTAGESGPAIVFLHGWGAFKELWWSALRDLGRDYRCFAIDMPGHGESRIGRADQIERIAVLIA
DFCHDLGLSTIILVGHSMGGSVAVEMTLHYPHLVQRLALIDAAVDAYRMPAYTRIYLLPHLGWPTFRLTQAIGRAFRPLG
QRIPHEHGGGWIRPWLRRASYLATFDPEGLYRILRSLFATRADERLQQIRVPTLVMTGQFDSLAPPAHARRLAQVIPGAR
YVMIPVSLHNPMDERPRAFTRALRAFLAETDS

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Homo sapiens, GI189027141, Length=301, Percent_Identity=25.2491694352159, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI218777837, Length=285, Percent_Identity=24.5614035087719, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 30906; Mature: 30775

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVQIMQHYHHRLGISYLTAGESGPAIVFLHGWGAFKELWWSALRDLGRDYRCFAIDMP
CCHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECC
GHGESRIGRADQIERIAVLIADFCHDLGLSTIILVGHSMGGSVAVEMTLHYPHLVQRLAL
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHH
IDAAVDAYRMPAYTRIYLLPHLGWPTFRLTQAIGRAFRPLGQRIPHEHGGGWIRPWLRRA
HHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SYLATFDPEGLYRILRSLFATRADERLQQIRVPTLVMTGQFDSLAPPAHARRLAQVIPGA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCC
RYVMIPVSLHNPMDERPRAFTRALRAFLAETDS
EEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIVQIMQHYHHRLGISYLTAGESGPAIVFLHGWGAFKELWWSALRDLGRDYRCFAIDMP
CHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECC
GHGESRIGRADQIERIAVLIADFCHDLGLSTIILVGHSMGGSVAVEMTLHYPHLVQRLAL
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHH
IDAAVDAYRMPAYTRIYLLPHLGWPTFRLTQAIGRAFRPLGQRIPHEHGGGWIRPWLRRA
HHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SYLATFDPEGLYRILRSLFATRADERLQQIRVPTLVMTGQFDSLAPPAHARRLAQVIPGA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCC
RYVMIPVSLHNPMDERPRAFTRALRAFLAETDS
EEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]