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The map label for this gene is mcrB [C]

Identifier: 222523853

GI number: 222523853

Start: 667743

End: 669365

Strand: Direct

Name: mcrB [C]

Synonym: Chy400_0561

Alternate gene names: 222523853

Gene position: 667743-669365 (Clockwise)

Preceding gene: 222523852

Following gene: 222523855

Centisome position: 12.67

GC content: 56.81

Gene sequence:

>1623_bases
ATGCCGTTCTCTTCTGCTGCATTGCATGCATTGTTACAACCCGGCGTTTCTGAACGCTGGCAGGCTGTGCAACGCCTGTT
ACACCCTGAGATGCTGGTACTGGCCAATCGCGCCGCAGAGCGTGCAGCGGCGCTCTTGCCACAACAATGGCCGCTGTACG
AATTGAGTTTTAAATCACGGCGGGCAATTGATCGCGGTGGTGGTCGGCGCGATCCCATCACCGACTACTGGTTTGCTTTT
GACCGACCGCCACGTGGGGCCGGTGTCATGCTCACGGTCAGCGGAATCGAGCGGACCGTCACTGTTGGGTTGCAATTGTG
GGGTGTACGCCGGCCACAACTTGCCCACCTCTGGAGTCAGGCGCGTCCGGTGTGGGAGGCACTGATTGATCGAATCGAAC
ACGAGGGTCAGGCTCGTTTTGCCGGTCGCCGCCCGCCGATGACAGGGATGCGCTGGATCGACCATTATCTCAACAGTCGA
GCACACTACCTGTGGGCAGGGTTCGTCTACGACTGGAATGCGCTCCCGACAGCAGAACGCCTGATCGACGACATTTGTGC
ACTGCTACCACTCAATGAAGCGTTGATGGAGCAAGCCGAAATCGATCTGGCAACGACAAATACCTTACGTGAAACACCAG
CTCGTTATCTGACAGGCGCGCCATCAGTAGCGCAGATCGTTATGTCGATTAAGCGGCGCGGTATGATTATTGACGAACGT
ACACTGCACGCTTTTCATCTTGCAGTACAGGCCCGACCGCTGGTCATCCTGGCCGGGCCGAGCGGGAGCGGCAAGACCTG
GCTGACGCGCCTTTACGCCGATGCCCTGATTGGCATTGGTGAAGGGCAGCCGAACCCCTGTTATCTTCTGGTTGCAGTAC
AACCCGACTGGCATAGTGCCCGCGATCTCCTCGGCTACTACAACTCGCTGACCGGCCTCTACCAGCCTACGCCATTCTTA
AACTTTCTCTTGAAAGCGGCTGCTGATCCGGATCAAACCTATATTGTCTGCCTCGATGAGATGAACCTGGCACGGCCCGA
ATACTACCTGGCGCCAATCCTGTCAGCAATGGAGGCGGTAGAGGGATTGATCGATCTGGGCACACCCCTGGCGGAGACAC
CATTAGCCGGTGGTGGTGTGGTACGCAACCCCATTCGTTTACCGATCAATCTACGCCTTATCGGAACAATCAATGTCGAT
GAAAGCACGTTTGCGCTGAGTGATAAAGTGCTCGACCGCGCCAACTTAATCGAATTAGGAACGGTCGATATGGCTGCCTT
GCGCTCACAGTATCCGATAGCAAACGATCAGGTCTGGCAGATATTGAGCGACATCCAGCACCACCTGACTGCTGCCGGAA
AGCCGGCAGGTTACCGCGCCTTGCGCGAGATACTTGGCTTCGTTGGGCAGGCAACAGATATGAACGTTTTAGAAGCACTC
GATCTTCAGATTCTGCAGCGCCTGCTACCGCGCTTGCGCGGTGAGGACTCCCCGCGCTTCCGACAGGCACTCAACGGCTT
ACATGCGATTTGCGCAGGGCGCCTCTCGCGAAGTGCCGAGCGTCTGGCCCAGATGATGGCTCGCCTTGAGCGGGAGGGAT
ATACCGACTTTTATGGATACTAG

Upstream 100 bases:

>100_bases
AATTACGCGAGATGTTGCCACGGCTCACAATCGTCTTCCATGCCGGTCAAAATGAATCAGGGCTTGATCCGCAGCTCTAC
TTACGCCCACCAACTGCCTG

Downstream 100 bases:

>100_bases
GAAAACCAGAATCCCTGATCGGTGAGTGGAACGACAACAGTGGGGGGAAGGCCAGAACGACTGAAGTAGCACAGGCCGCG
GCCTGTGCTACTTCTAGTGA

Product: ATPase

Products: NA

Alternate protein names: GTPase Subunit Of Restriction Endonuclease-Like Protein; 5-Methylcytosine-Specific Restriction ; GTPase Subunit Of Restriction Endonuclease-Like; Site-Specific DNA-Methyltransferase Cytosine-Specific; 5-Methylcytosine-Specific Restriction McrB Related Protein; AAA Family ATPase; AAA ATPase; 5-Methylcytosine-Specific Restriction-Like Protein; ATPase AAA Family

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MPFSSAALHALLQPGVSERWQAVQRLLHPEMLVLANRAAERAAALLPQQWPLYELSFKSRRAIDRGGGRRDPITDYWFAF
DRPPRGAGVMLTVSGIERTVTVGLQLWGVRRPQLAHLWSQARPVWEALIDRIEHEGQARFAGRRPPMTGMRWIDHYLNSR
AHYLWAGFVYDWNALPTAERLIDDICALLPLNEALMEQAEIDLATTNTLRETPARYLTGAPSVAQIVMSIKRRGMIIDER
TLHAFHLAVQARPLVILAGPSGSGKTWLTRLYADALIGIGEGQPNPCYLLVAVQPDWHSARDLLGYYNSLTGLYQPTPFL
NFLLKAAADPDQTYIVCLDEMNLARPEYYLAPILSAMEAVEGLIDLGTPLAETPLAGGGVVRNPIRLPINLRLIGTINVD
ESTFALSDKVLDRANLIELGTVDMAALRSQYPIANDQVWQILSDIQHHLTAAGKPAGYRALREILGFVGQATDMNVLEAL
DLQILQRLLPRLRGEDSPRFRQALNGLHAICAGRLSRSAERLAQMMARLEREGYTDFYGY

Sequences:

>Translated_540_residues
MPFSSAALHALLQPGVSERWQAVQRLLHPEMLVLANRAAERAAALLPQQWPLYELSFKSRRAIDRGGGRRDPITDYWFAF
DRPPRGAGVMLTVSGIERTVTVGLQLWGVRRPQLAHLWSQARPVWEALIDRIEHEGQARFAGRRPPMTGMRWIDHYLNSR
AHYLWAGFVYDWNALPTAERLIDDICALLPLNEALMEQAEIDLATTNTLRETPARYLTGAPSVAQIVMSIKRRGMIIDER
TLHAFHLAVQARPLVILAGPSGSGKTWLTRLYADALIGIGEGQPNPCYLLVAVQPDWHSARDLLGYYNSLTGLYQPTPFL
NFLLKAAADPDQTYIVCLDEMNLARPEYYLAPILSAMEAVEGLIDLGTPLAETPLAGGGVVRNPIRLPINLRLIGTINVD
ESTFALSDKVLDRANLIELGTVDMAALRSQYPIANDQVWQILSDIQHHLTAAGKPAGYRALREILGFVGQATDMNVLEAL
DLQILQRLLPRLRGEDSPRFRQALNGLHAICAGRLSRSAERLAQMMARLEREGYTDFYGY
>Mature_539_residues
PFSSAALHALLQPGVSERWQAVQRLLHPEMLVLANRAAERAAALLPQQWPLYELSFKSRRAIDRGGGRRDPITDYWFAFD
RPPRGAGVMLTVSGIERTVTVGLQLWGVRRPQLAHLWSQARPVWEALIDRIEHEGQARFAGRRPPMTGMRWIDHYLNSRA
HYLWAGFVYDWNALPTAERLIDDICALLPLNEALMEQAEIDLATTNTLRETPARYLTGAPSVAQIVMSIKRRGMIIDERT
LHAFHLAVQARPLVILAGPSGSGKTWLTRLYADALIGIGEGQPNPCYLLVAVQPDWHSARDLLGYYNSLTGLYQPTPFLN
FLLKAAADPDQTYIVCLDEMNLARPEYYLAPILSAMEAVEGLIDLGTPLAETPLAGGGVVRNPIRLPINLRLIGTINVDE
STFALSDKVLDRANLIELGTVDMAALRSQYPIANDQVWQILSDIQHHLTAAGKPAGYRALREILGFVGQATDMNVLEALD
LQILQRLLPRLRGEDSPRFRQALNGLHAICAGRLSRSAERLAQMMARLEREGYTDFYGY

Specific function: Recognizes N4- And C5-Methylcytosine (And 5-Hydroxy- Methylcytosines) Produced By A Broad Range Of DNA Methylases And Appears To Act Against 5-Methylcytosine Preceded By A Purine Residue. Binds To DNA Containing Methylated Cytosines; Also Binds To GTP. [

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.21.- [C]

Molecular weight: Translated: 60315; Mature: 60184

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSSAALHALLQPGVSERWQAVQRLLHPEMLVLANRAAERAAALLPQQWPLYELSFKSR
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHH
RAIDRGGGRRDPITDYWFAFDRPPRGAGVMLTVSGIERTVTVGLQLWGVRRPQLAHLWSQ
HHHHCCCCCCCCCCHHHEEECCCCCCCEEEEEECCCCEEHEEHHHHCCCCCHHHHHHHHH
ARPVWEALIDRIEHEGQARFAGRRPPMTGMRWIDHYLNSRAHYLWAGFVYDWNALPTAER
CCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHH
LIDDICALLPLNEALMEQAEIDLATTNTLRETPARYLTGAPSVAQIVMSIKRRGMIIDER
HHHHHHHHHCCCHHHHHHHHCCHHCCHHHHHCCHHHHCCCCHHHHHHHHHHHCCCEEECH
TLHAFHLAVQARPLVILAGPSGSGKTWLTRLYADALIGIGEGQPNPCYLLVAVQPDWHSA
HHHHHHHEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHH
RDLLGYYNSLTGLYQPTPFLNFLLKAAADPDQTYIVCLDEMNLARPEYYLAPILSAMEAV
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHH
EGLIDLGTPLAETPLAGGGVVRNPIRLPINLRLIGTINVDESTFALSDKVLDRANLIELG
HHHHHCCCCCCCCCCCCCCEECCCEECEEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEC
TVDMAALRSQYPIANDQVWQILSDIQHHLTAAGKPAGYRALREILGFVGQATDMNVLEAL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
DLQILQRLLPRLRGEDSPRFRQALNGLHAICAGRLSRSAERLAQMMARLEREGYTDFYGY
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PFSSAALHALLQPGVSERWQAVQRLLHPEMLVLANRAAERAAALLPQQWPLYELSFKSR
CCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHH
RAIDRGGGRRDPITDYWFAFDRPPRGAGVMLTVSGIERTVTVGLQLWGVRRPQLAHLWSQ
HHHHCCCCCCCCCCHHHEEECCCCCCCEEEEEECCCCEEHEEHHHHCCCCCHHHHHHHHH
ARPVWEALIDRIEHEGQARFAGRRPPMTGMRWIDHYLNSRAHYLWAGFVYDWNALPTAER
CCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHH
LIDDICALLPLNEALMEQAEIDLATTNTLRETPARYLTGAPSVAQIVMSIKRRGMIIDER
HHHHHHHHHCCCHHHHHHHHCCHHCCHHHHHCCHHHHCCCCHHHHHHHHHHHCCCEEECH
TLHAFHLAVQARPLVILAGPSGSGKTWLTRLYADALIGIGEGQPNPCYLLVAVQPDWHSA
HHHHHHHEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHH
RDLLGYYNSLTGLYQPTPFLNFLLKAAADPDQTYIVCLDEMNLARPEYYLAPILSAMEAV
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHH
EGLIDLGTPLAETPLAGGGVVRNPIRLPINLRLIGTINVDESTFALSDKVLDRANLIELG
HHHHHCCCCCCCCCCCCCCEECCCEECEEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEC
TVDMAALRSQYPIANDQVWQILSDIQHHLTAAGKPAGYRALREILGFVGQATDMNVLEAL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
DLQILQRLLPRLRGEDSPRFRQALNGLHAICAGRLSRSAERLAQMMARLEREGYTDFYGY
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA