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The map label for this gene is folC [H]

Identifier: 222523759

GI number: 222523759

Start: 563823

End: 565139

Strand: Direct

Name: folC [H]

Synonym: Chy400_0465

Alternate gene names: 222523759

Gene position: 563823-565139 (Clockwise)

Preceding gene: 222523752

Following gene: 222523762

Centisome position: 10.7

GC content: 61.05

Gene sequence:

>1317_bases
ATGCAGATTACAACCTATCAGGAAGCTCTCGATTACATCTACAGTTTTATCGATCCTACTCGTCAGGGGTCACCCGATCC
GGCGATTGCGGCGCGATCGCTGGCCCGGCTCAGGGCATTACTAGACATTGCCGGGAATCCACATCAACAACTGGCGGCGG
TTGTCGTTGCCGGTACCAAAGGGAAGGGGAGTACCTGCGCCTTGATTGAAGCTATGGCTCGCGCCGCCGGTTTACGGGTG
GGTTTATGGACATCGCCCCACCTTAGCTCGTATCGCGAACGCATCCAGATTGATCGCGAACCGATCTCTCAGGCGACGCT
GATTCAGCTTGTGCGGGATGTGCAACCGATTGTTGACGCCTTTGACACCGCTACCTACGGTCGTCCCAGTACGTTTGACA
TTGGGTTTGTGATGGCGATGCGCCATTTTGTAGCAGCTCACGTCGATCTGGCGGTCGTTGAGGTCGGTCTGGGGGGTCGT
TTCGATGCTGCGGCGACCATTACTCCACTCATCGCGGTGATCTCGTCGATCAGCTACGACCACATGGCGATTCTGGGGCC
AACGCTGCACGACATTGCCTACAACAAAGCGGGTATTATGCGGCCAGGCCGACCGGCGATTACCGTACCACAACACCCGG
AAGCAGCCGCTGTCTTCGCCGCCGAAGCCCAGACGGTCGGTGCCCCGCTCTGGCTGGCCGCTGAACACACGGTTGAACCG
TGGCTGGCGTCTGGTCAACCGCTCACCTATCCGGCCCCACCGCAACCGGGGCGCTTGCGCGGTGGCTTCCAGCGCGAAAA
TGCTCGTCTGGCAATGGGGACTGCCCTGCTGTTGCGTGATCAGGGAGTGCCGATTGATGACCAGGCCATCCGGCGTGGTC
TCGCCGAAGCCTGGTGGCCGGGACGTTTCGAGTTGATTGCCGGCCCGCCCCGTATCCTTATCGACGGCGCCCACAACGGC
GACTCGGCGCAGAAATTGTGGCAGGCCATTAGCGACGAGCTACCGCATCGGCAGTTTATTCTCGTGCTCGGTACCTCGCG
CGATAAAGACATTGCGGCTATCGCCGCTGCCCTGGCACCGCAGGCAGCCCACGTGATTATCACCCGCTCGAACCACCCCA
AAGCGATGGATCTCGACCGGATTGCCGCCGAGGTTGCCCCCTTTACCAACGCACCAATGCAGATTGTGCCGGTCGTGGCT
GAAGCGCTGGCGACCGCGCAGGCGCTGGCGGAACAGGACGATCTGATTTGTGTCACCGGCTCGCTGTTCGTCGTCGGCGC
AGCCCGTGAAGCCCTGGGCCTGGCGGTCGCAGACTGA

Upstream 100 bases:

>100_bases
CTCAGACCACCACCACCTTCCGGTCAATGGTATGATGGATCAATAAATTGTTGCGGTTACCGGCTGGTTCCGGGCAAGCA
CAGGGCAGGAAAAGCAGGAT

Downstream 100 bases:

>100_bases
GGGTAAGCGGTTGGTAGCGGCGGGTTGTTCAGCGGTGACGCAGCAGCGCTACGACGAAGCGCTGGTATACGAGCGCAGAC
CAACCCGCCAGAAAGCGCGT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQITTYQEALDYIYSFIDPTRQGSPDPAIAARSLARLRALLDIAGNPHQQLAAVVVAGTKGKGSTCALIEAMARAAGLRV
GLWTSPHLSSYRERIQIDREPISQATLIQLVRDVQPIVDAFDTATYGRPSTFDIGFVMAMRHFVAAHVDLAVVEVGLGGR
FDAAATITPLIAVISSISYDHMAILGPTLHDIAYNKAGIMRPGRPAITVPQHPEAAAVFAAEAQTVGAPLWLAAEHTVEP
WLASGQPLTYPAPPQPGRLRGGFQRENARLAMGTALLLRDQGVPIDDQAIRRGLAEAWWPGRFELIAGPPRILIDGAHNG
DSAQKLWQAISDELPHRQFILVLGTSRDKDIAAIAAALAPQAAHVIITRSNHPKAMDLDRIAAEVAPFTNAPMQIVPVVA
EALATAQALAEQDDLICVTGSLFVVGAAREALGLAVAD

Sequences:

>Translated_438_residues
MQITTYQEALDYIYSFIDPTRQGSPDPAIAARSLARLRALLDIAGNPHQQLAAVVVAGTKGKGSTCALIEAMARAAGLRV
GLWTSPHLSSYRERIQIDREPISQATLIQLVRDVQPIVDAFDTATYGRPSTFDIGFVMAMRHFVAAHVDLAVVEVGLGGR
FDAAATITPLIAVISSISYDHMAILGPTLHDIAYNKAGIMRPGRPAITVPQHPEAAAVFAAEAQTVGAPLWLAAEHTVEP
WLASGQPLTYPAPPQPGRLRGGFQRENARLAMGTALLLRDQGVPIDDQAIRRGLAEAWWPGRFELIAGPPRILIDGAHNG
DSAQKLWQAISDELPHRQFILVLGTSRDKDIAAIAAALAPQAAHVIITRSNHPKAMDLDRIAAEVAPFTNAPMQIVPVVA
EALATAQALAEQDDLICVTGSLFVVGAAREALGLAVAD
>Mature_438_residues
MQITTYQEALDYIYSFIDPTRQGSPDPAIAARSLARLRALLDIAGNPHQQLAAVVVAGTKGKGSTCALIEAMARAAGLRV
GLWTSPHLSSYRERIQIDREPISQATLIQLVRDVQPIVDAFDTATYGRPSTFDIGFVMAMRHFVAAHVDLAVVEVGLGGR
FDAAATITPLIAVISSISYDHMAILGPTLHDIAYNKAGIMRPGRPAITVPQHPEAAAVFAAEAQTVGAPLWLAAEHTVEP
WLASGQPLTYPAPPQPGRLRGGFQRENARLAMGTALLLRDQGVPIDDQAIRRGLAEAWWPGRFELIAGPPRILIDGAHNG
DSAQKLWQAISDELPHRQFILVLGTSRDKDIAAIAAALAPQAAHVIITRSNHPKAMDLDRIAAEVAPFTNAPMQIVPVVA
EALATAQALAEQDDLICVTGSLFVVGAAREALGLAVAD

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=374, Percent_Identity=32.8877005347594, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI66932984, Length=374, Percent_Identity=32.8877005347594, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1788654, Length=387, Percent_Identity=30.2325581395349, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71984923, Length=435, Percent_Identity=27.1264367816092, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17553148, Length=435, Percent_Identity=27.1264367816092, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17553150, Length=435, Percent_Identity=27.1264367816092, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323760, Length=421, Percent_Identity=26.8408551068884, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324815, Length=436, Percent_Identity=27.2935779816514, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24641571, Length=440, Percent_Identity=28.4090909090909, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=35.195530726257, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46722; Mature: 46722

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITTYQEALDYIYSFIDPTRQGSPDPAIAARSLARLRALLDIAGNPHQQLAAVVVAGTK
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCC
GKGSTCALIEAMARAAGLRVGLWTSPHLSSYRERIQIDREPISQATLIQLVRDVQPIVDA
CCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FDTATYGRPSTFDIGFVMAMRHFVAAHVDLAVVEVGLGGRFDAAATITPLIAVISSISYD
HHCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
HMAILGPTLHDIAYNKAGIMRPGRPAITVPQHPEAAAVFAAEAQTVGAPLWLAAEHTVEP
CEEEECCHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEHHHHHCCCCEEEEECCCCCC
WLASGQPLTYPAPPQPGRLRGGFQRENARLAMGTALLLRDQGVPIDDQAIRRGLAEAWWP
HHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCC
GRFELIAGPPRILIDGAHNGDSAQKLWQAISDELPHRQFILVLGTSRDKDIAAIAAALAP
CCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCC
QAAHVIITRSNHPKAMDLDRIAAEVAPFTNAPMQIVPVVAEALATAQALAEQDDLICVTG
CCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
SLFVVGAAREALGLAVAD
CEEEEECHHHHHCEEECC
>Mature Secondary Structure
MQITTYQEALDYIYSFIDPTRQGSPDPAIAARSLARLRALLDIAGNPHQQLAAVVVAGTK
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCC
GKGSTCALIEAMARAAGLRVGLWTSPHLSSYRERIQIDREPISQATLIQLVRDVQPIVDA
CCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FDTATYGRPSTFDIGFVMAMRHFVAAHVDLAVVEVGLGGRFDAAATITPLIAVISSISYD
HHCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
HMAILGPTLHDIAYNKAGIMRPGRPAITVPQHPEAAAVFAAEAQTVGAPLWLAAEHTVEP
CEEEECCHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEHHHHHCCCCEEEEECCCCCC
WLASGQPLTYPAPPQPGRLRGGFQRENARLAMGTALLLRDQGVPIDDQAIRRGLAEAWWP
HHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCC
GRFELIAGPPRILIDGAHNGDSAQKLWQAISDELPHRQFILVLGTSRDKDIAAIAAALAP
CCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCC
QAAHVIITRSNHPKAMDLDRIAAEVAPFTNAPMQIVPVVAEALATAQALAEQDDLICVTG
CCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
SLFVVGAAREALGLAVAD
CEEEEECHHHHHCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]