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The map label for this gene is pheS [H]

Identifier: 222523707

GI number: 222523707

Start: 486699

End: 487748

Strand: Direct

Name: pheS [H]

Synonym: Chy400_0413

Alternate gene names: 222523707

Gene position: 486699-487748 (Clockwise)

Preceding gene: 222523706

Following gene: 222523708

Centisome position: 9.24

GC content: 58.0

Gene sequence:

>1050_bases
ATGACCACACTGCTCGATGAATTGACAACTCTCGAACAAGAAGCCCTGACCGCGCTTGAGACTGTTGCCGACTTGTCTGC
ACTCACCGAATGGAAGAGCCGCTTTATCGGCAAACAGGGTCGTCTGTCGCGTCTCAGCCGGGGGCTTGGTGCATTGCCGG
CCTCCGAACGCCCACTGGTCGGTCAGCGGGTCAATGCCTTGCGCGAGCAACTCGAAGCAGCGTTCAGCGCTGCGAAAGAG
CGCCTGGAAGCACAGGCCGTTGCTGCTGAGCTGGCCGCCGAGCGCATCGACGTTACAATGCCCGGTCGTCGCCCTGCCGT
CGGTTATGTCCATCTGACCAACCAGATTCTGCGCCAGGTTCAGCATATCTTTGCCGAGATGGGGTTTCAGGTTTGGGAAA
GCCCTGAAGTTGAGTACGACGCATTCAATTTCAGTTTGCTAAACTTCCCCGATGACCATCCGGCCCGCGATATGCAGGAC
ACGTTTTATGTCGAGATGCCGGCTGGCGCACCTTCCGTACTCTTGCGCACCCACACCTCACCCGGTCAGATCCATGCTAT
GCGCCGCAATGCGCCCAATCCGCTGCGCGTACTCATCCCCGGCAAGGTCTACCGCAACGAACAGGTAACGGTACGCAGCG
AGATGATGTTCCACCAGTTCGAGTTTCTCGCGGTTGGGCGGAATGTCACGATGGCTGATTTGAAGGGCACGCTCAGCTTT
TTCGCCGAACGCATGTTTGGTCCAGGCACCCGGGTTCGCCTGCGCCCCAGTTTCTTCCCCTTCACCGAACCCAGTGCCGA
GATGGATGTGACCTGCTTCCTCTGCGGTGGGAAGGGCTGTCGGATTTGCAAATATGCCGGCTGGCTCGAAATTGGCGGGT
GCGGGATGGTACATCCAAACGTGCTGCGCAACGGCGGGTATGATCCGGCAGAGTTCAGTGGTTTCGCGGGTGGGTTTGGC
CCCGAACGAGTAGCGATGCTCAAATACGGTATCGACGATATTCGCTGGTTCTATAGCGGCGACCAACGCTTTGTTGAACA
GTTCGGATAA

Upstream 100 bases:

>100_bases
ACACCTGCTGGTGTAGCCCACCATTGTTTGTTACACTAACCCGAACTCGGATTATAATAGCAGAGAGATACCACTTGCCG
CTAATAATCTATGGTGTCAC

Downstream 100 bases:

>100_bases
GGTATGCGTAGAGATGAATTGAACCGGGCCATCCACGATCTCTACCGCGCTGAACACGAAGCCCTTGGCGAGCGCGGTGG
CTACGAGTTGCTGGAACGCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTTLLDELTTLEQEALTALETVADLSALTEWKSRFIGKQGRLSRLSRGLGALPASERPLVGQRVNALREQLEAAFSAAKE
RLEAQAVAAELAAERIDVTMPGRRPAVGYVHLTNQILRQVQHIFAEMGFQVWESPEVEYDAFNFSLLNFPDDHPARDMQD
TFYVEMPAGAPSVLLRTHTSPGQIHAMRRNAPNPLRVLIPGKVYRNEQVTVRSEMMFHQFEFLAVGRNVTMADLKGTLSF
FAERMFGPGTRVRLRPSFFPFTEPSAEMDVTCFLCGGKGCRICKYAGWLEIGGCGMVHPNVLRNGGYDPAEFSGFAGGFG
PERVAMLKYGIDDIRWFYSGDQRFVEQFG

Sequences:

>Translated_349_residues
MTTLLDELTTLEQEALTALETVADLSALTEWKSRFIGKQGRLSRLSRGLGALPASERPLVGQRVNALREQLEAAFSAAKE
RLEAQAVAAELAAERIDVTMPGRRPAVGYVHLTNQILRQVQHIFAEMGFQVWESPEVEYDAFNFSLLNFPDDHPARDMQD
TFYVEMPAGAPSVLLRTHTSPGQIHAMRRNAPNPLRVLIPGKVYRNEQVTVRSEMMFHQFEFLAVGRNVTMADLKGTLSF
FAERMFGPGTRVRLRPSFFPFTEPSAEMDVTCFLCGGKGCRICKYAGWLEIGGCGMVHPNVLRNGGYDPAEFSGFAGGFG
PERVAMLKYGIDDIRWFYSGDQRFVEQFG
>Mature_348_residues
TTLLDELTTLEQEALTALETVADLSALTEWKSRFIGKQGRLSRLSRGLGALPASERPLVGQRVNALREQLEAAFSAAKER
LEAQAVAAELAAERIDVTMPGRRPAVGYVHLTNQILRQVQHIFAEMGFQVWESPEVEYDAFNFSLLNFPDDHPARDMQDT
FYVEMPAGAPSVLLRTHTSPGQIHAMRRNAPNPLRVLIPGKVYRNEQVTVRSEMMFHQFEFLAVGRNVTMADLKGTLSFF
AERMFGPGTRVRLRPSFFPFTEPSAEMDVTCFLCGGKGCRICKYAGWLEIGGCGMVHPNVLRNGGYDPAEFSGFAGGFGP
ERVAMLKYGIDDIRWFYSGDQRFVEQFG

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=275, Percent_Identity=30.5454545454545, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI5729820, Length=241, Percent_Identity=29.8755186721992, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1788007, Length=344, Percent_Identity=43.3139534883721, Blast_Score=279, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI32563657, Length=272, Percent_Identity=29.0441176470588, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17508957, Length=272, Percent_Identity=29.0441176470588, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32566635, Length=289, Percent_Identity=30.4498269896194, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321087, Length=378, Percent_Identity=26.4550264550265, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6325304, Length=99, Percent_Identity=39.3939393939394, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI18858079, Length=276, Percent_Identity=30.0724637681159, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137424, Length=255, Percent_Identity=26.6666666666667, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39012; Mature: 38881

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLLDELTTLEQEALTALETVADLSALTEWKSRFIGKQGRLSRLSRGLGALPASERPLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCH
GQRVNALREQLEAAFSAAKERLEAQAVAAELAAERIDVTMPGRRPAVGYVHLTNQILRQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEEHHHHHHHHHH
QHIFAEMGFQVWESPEVEYDAFNFSLLNFPDDHPARDMQDTFYVEMPAGAPSVLLRTHTS
HHHHHHHCHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEECCC
PGQIHAMRRNAPNPLRVLIPGKVYRNEQVTVRSEMMFHQFEFLAVGRNVTMADLKGTLSF
CCCEEEHHCCCCCCEEEEECCEEECCCCEEHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
FAERMFGPGTRVRLRPSFFPFTEPSAEMDVTCFLCGGKGCRICKYAGWLEIGGCGMVHPN
HHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCEEEECCCCCCCCH
VLRNGGYDPAEFSGFAGGFGPERVAMLKYGIDDIRWFYSGDQRFVEQFG
HHHCCCCCCHHHCCCCCCCCHHHHHHHHHCHHHHHEEECCHHHHHHHCC
>Mature Secondary Structure 
TTLLDELTTLEQEALTALETVADLSALTEWKSRFIGKQGRLSRLSRGLGALPASERPLV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCH
GQRVNALREQLEAAFSAAKERLEAQAVAAELAAERIDVTMPGRRPAVGYVHLTNQILRQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEEHHHHHHHHHH
QHIFAEMGFQVWESPEVEYDAFNFSLLNFPDDHPARDMQDTFYVEMPAGAPSVLLRTHTS
HHHHHHHCHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEECCC
PGQIHAMRRNAPNPLRVLIPGKVYRNEQVTVRSEMMFHQFEFLAVGRNVTMADLKGTLSF
CCCEEEHHCCCCCCEEEEECCEEECCCCEEHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
FAERMFGPGTRVRLRPSFFPFTEPSAEMDVTCFLCGGKGCRICKYAGWLEIGGCGMVHPN
HHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCEEEECCCCCCCCH
VLRNGGYDPAEFSGFAGGFGPERVAMLKYGIDDIRWFYSGDQRFVEQFG
HHHCCCCCCHHHCCCCCCCCHHHHHHHHHCHHHHHEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA