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The map label for this gene is tetA [H]

Identifier: 222523679

GI number: 222523679

Start: 452539

End: 453750

Strand: Direct

Name: tetA [H]

Synonym: Chy400_0385

Alternate gene names: 222523679

Gene position: 452539-453750 (Clockwise)

Preceding gene: 222523678

Following gene: 222523680

Centisome position: 8.59

GC content: 59.82

Gene sequence:

>1212_bases
GTGAGTCGGCACCATGTCATCGTCGCCGGTTCTGTGCTGGTCGATATGCTAGGGTACGGCCTGATCATGCCGTTGTTGCC
TTTTATCGTCAAAACCTGGGTGAGCGACGCCACAGTGATCGGTTTGCTTGGCTCGTTGTATGCAGCGATGCAATTACTGG
CAGCACCACTCCTGGGCACCCTCTCTGACCGCATCGGACGCCGATCTGTCTTACTGATCTGCCTGTTTGGTTCAGCGGTA
GCCTACCTCGGTCTGGCGCTGGCCGGCTCACTGCCGCTGGTCATCCTGGCTATCGCGCTGGGTGGCGCAGCCGGATCGAG
CATGCCGGTAGCGCAGGCCTATATCGCCGATACCACCACCGCAGAGCGACGCGCACAAGGATTTGGGTTGCTGGGGGCAG
CCTTCGGCCTGGGACTAATCGGCGGTTCGGCCATCGGCGGGTTGCTGAGTCAGTATGGATTAGCGCTACCACCTGCGGTA
GCGGCGACGATTGCCTTCCTTAATGCACTCTGGGCAACCATCGCCCTGCCCGAAACCCTGCCACCCAATCGGCGACGCAT
CCAACCGGTGCGGCTGACAAACCCCTTTGCAGCAGCCGTGACCGTTCTGACCCTGCCGCAGATACGTCCGCTCCTTGGCG
CAGTTGTCATCCTCAACATCGCCTTCGCCGGGTTACAGAACAACGTTGCCTACTTCACCATGACCCGCTTTGGCTGGGGA
CCGGAACAAAATGCCGTCCTCTTCGTATTCGTTGGTCTGTGCGCCGTGGTTACCCAGGGCGTCCTGCTCAGGATACTGCA
ACCGTTGCTCGGTGAGAGGCACCTTGCCGGTGGCGGACTGGGCTTGATGGCTCTCGGCTTTGCCCTCGTGGGGCTGGTGC
ACAATGAAAGCTGGCGACTCTTTCCCATTGCTGCCATGTTTGCAACCGGCATGGGATTAGCAGTTCCCGCCCTGACGAGC
CTGATCTCGCTCCGGGCCGGTGATCGTCGCCAGGGAGCCATTCTCGGCGGTATGCAGACCCTGATCAGCATCGCACTGGT
GATCGGCCCATTGAGCTTCGGTTTCATCTTCGACCAGATCGGCCCCGATGCTCCCTATCTGATTGGGAGCTTGATGCTGC
TCGGTGCATGGCTGATAACGGTACTAACCTTCGTGCAACCAACGCCGGTTACCACCGGTTCATTGCATCCAGTTGAAGAC
TCAGAGGCTTAG

Upstream 100 bases:

>100_bases
TCAGCGCTCCCTGCAGGGCCGACCATCAGATGCGTGCAACCGTCCTTCACACTGCCCGGCAATCTATCTACCAGGCTCGC
GCCGCGCAAAGGAGAACACC

Downstream 100 bases:

>100_bases
ATATGACAACGTTCTTTCCCATCACCCGTGCTGCTATGGCTGACTTACCAACCCGCTTCGGTCACTTTCAGATTGTGGTC
TATCTCGACGCAGAGCAGAA

Product: major facilitator superfamily protein

Products: NA

Alternate protein names: TetA(B); Metal-tetracycline/H(+) antiporter [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSRHHVIVAGSVLVDMLGYGLIMPLLPFIVKTWVSDATVIGLLGSLYAAMQLLAAPLLGTLSDRIGRRSVLLICLFGSAV
AYLGLALAGSLPLVILAIALGGAAGSSMPVAQAYIADTTTAERRAQGFGLLGAAFGLGLIGGSAIGGLLSQYGLALPPAV
AATIAFLNALWATIALPETLPPNRRRIQPVRLTNPFAAAVTVLTLPQIRPLLGAVVILNIAFAGLQNNVAYFTMTRFGWG
PEQNAVLFVFVGLCAVVTQGVLLRILQPLLGERHLAGGGLGLMALGFALVGLVHNESWRLFPIAAMFATGMGLAVPALTS
LISLRAGDRRQGAILGGMQTLISIALVIGPLSFGFIFDQIGPDAPYLIGSLMLLGAWLITVLTFVQPTPVTTGSLHPVED
SEA

Sequences:

>Translated_403_residues
MSRHHVIVAGSVLVDMLGYGLIMPLLPFIVKTWVSDATVIGLLGSLYAAMQLLAAPLLGTLSDRIGRRSVLLICLFGSAV
AYLGLALAGSLPLVILAIALGGAAGSSMPVAQAYIADTTTAERRAQGFGLLGAAFGLGLIGGSAIGGLLSQYGLALPPAV
AATIAFLNALWATIALPETLPPNRRRIQPVRLTNPFAAAVTVLTLPQIRPLLGAVVILNIAFAGLQNNVAYFTMTRFGWG
PEQNAVLFVFVGLCAVVTQGVLLRILQPLLGERHLAGGGLGLMALGFALVGLVHNESWRLFPIAAMFATGMGLAVPALTS
LISLRAGDRRQGAILGGMQTLISIALVIGPLSFGFIFDQIGPDAPYLIGSLMLLGAWLITVLTFVQPTPVTTGSLHPVED
SEA
>Mature_402_residues
SRHHVIVAGSVLVDMLGYGLIMPLLPFIVKTWVSDATVIGLLGSLYAAMQLLAAPLLGTLSDRIGRRSVLLICLFGSAVA
YLGLALAGSLPLVILAIALGGAAGSSMPVAQAYIADTTTAERRAQGFGLLGAAFGLGLIGGSAIGGLLSQYGLALPPAVA
ATIAFLNALWATIALPETLPPNRRRIQPVRLTNPFAAAVTVLTLPQIRPLLGAVVILNIAFAGLQNNVAYFTMTRFGWGP
EQNAVLFVFVGLCAVVTQGVLLRILQPLLGERHLAGGGLGLMALGFALVGLVHNESWRLFPIAAMFATGMGLAVPALTSL
ISLRAGDRRQGAILGGMQTLISIALVIGPLSFGFIFDQIGPDAPYLIGSLMLLGAWLITVLTFVQPTPVTTGSLHPVEDS
EA

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=363, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI94721311, Length=363, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI217416366, Length=186, Percent_Identity=31.7204301075269, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI225703104, Length=360, Percent_Identity=25.5555555555556, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI225703102, Length=360, Percent_Identity=25.5555555555556, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17551570, Length=407, Percent_Identity=24.8157248157248, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71996331, Length=370, Percent_Identity=23.5135135135135, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17738085, Length=355, Percent_Identity=29.5774647887324, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357569, Length=375, Percent_Identity=26.1333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24656463, Length=378, Percent_Identity=26.1904761904762, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24656468, Length=378, Percent_Identity=26.1904761904762, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI161080926, Length=378, Percent_Identity=26.1904761904762, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI161080924, Length=378, Percent_Identity=26.1904761904762, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI221513297, Length=359, Percent_Identity=27.0194986072423, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667561, Length=189, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24667557, Length=143, Percent_Identity=30.0699300699301, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41826; Mature: 41694

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRHHVIVAGSVLVDMLGYGLIMPLLPFIVKTWVSDATVIGLLGSLYAAMQLLAAPLLGT
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LSDRIGRRSVLLICLFGSAVAYLGLALAGSLPLVILAIALGGAAGSSMPVAQAYIADTTT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCHH
AERRAQGFGLLGAAFGLGLIGGSAIGGLLSQYGLALPPAVAATIAFLNALWATIALPETL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PPNRRRIQPVRLTNPFAAAVTVLTLPQIRPLLGAVVILNIAFAGLQNNVAYFTMTRFGWG
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCC
PEQNAVLFVFVGLCAVVTQGVLLRILQPLLGERHLAGGGLGLMALGFALVGLVHNESWRL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
FPIAAMFATGMGLAVPALTSLISLRAGDRRQGAILGGMQTLISIALVIGPLSFGFIFDQI
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPDAPYLIGSLMLLGAWLITVLTFVQPTPVTTGSLHPVEDSEA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRHHVIVAGSVLVDMLGYGLIMPLLPFIVKTWVSDATVIGLLGSLYAAMQLLAAPLLGT
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LSDRIGRRSVLLICLFGSAVAYLGLALAGSLPLVILAIALGGAAGSSMPVAQAYIADTTT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCHH
AERRAQGFGLLGAAFGLGLIGGSAIGGLLSQYGLALPPAVAATIAFLNALWATIALPETL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PPNRRRIQPVRLTNPFAAAVTVLTLPQIRPLLGAVVILNIAFAGLQNNVAYFTMTRFGWG
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCC
PEQNAVLFVFVGLCAVVTQGVLLRILQPLLGERHLAGGGLGLMALGFALVGLVHNESWRL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
FPIAAMFATGMGLAVPALTSLISLRAGDRRQGAILGGMQTLISIALVIGPLSFGFIFDQI
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPDAPYLIGSLMLLGAWLITVLTFVQPTPVTTGSLHPVEDSEA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6319234; 6298728; 1848846; 2168416; 8995300 [H]