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The map label for this gene is lysX [H]

Identifier: 222523653

GI number: 222523653

Start: 423796

End: 424644

Strand: Direct

Name: lysX [H]

Synonym: Chy400_0359

Alternate gene names: 222523653

Gene position: 423796-424644 (Clockwise)

Preceding gene: 222523652

Following gene: 222523654

Centisome position: 8.04

GC content: 56.54

Gene sequence:

>849_bases
ATGCGCATTGCTGTTCTTTGTTCGCGGATTCGCGCTGAAGAAAAACTGCTCTTCCAGGAGCTGGAGCGCCGTGGTCTCGA
TTATGTCAAAGTTGACGACCGCGAGCAGATTTTTGATCTGCATACCACAACCTATCCCTTCGATGTTGTACTTGAGCGCT
GCATCCAGCATAGCCGGGCCCTCTACATGCTCAAAATCTTCAACGATGCCGGCGTGCCTACGGTCAACACCTATCACGTA
GCGCTCACCTGTGGCGACAAATTCCTGACCACGCAGGCCCTTATTCATGCCGGCGTCCCCACACCACGCTGCCTGCTGGC
ATTCACCCCGGAAAGTGCCCTCGAAGCCATGGAGCAACTCGGCTATCCGGTTGTGCTCAAGCCGGTGATCGGCTCGTGGG
GGCGGATGGTGAGCAAGATCAATGATCGCGATGCTGCCGAAGCCATTCTCGAACATCGTGATGTTCTGGGCAACTATCAA
CACTCCATTTTTTACATTCAGCAGTACATTCACAAACCGGGGCGCGACATTCGCAGCTTTGTGATCGGCGATGAGTGTAT
CGCCGCAATTTACCGCACCAGCCCGCACTGGATCACCAACACTGCACGCGGTGGCGTTGCCACCAACTGCCCGGTTACCC
CAGAACTGGCCGACATCAGCGTGCGTGCTGCCCACGCCGTCGGCGGTGGGGTAGTGGCGATTGACTTGCTGGAAACGCCC
GAAGGTGAGCTGCTGGTTAATGAAGTCAACTACACCATGGAGTTCCGCAATAGTATCGACACCACCGGCGTTGATATTCC
GGCCCGGATTATCGACTATGTGCTAGAGGTTGGGCGCGGGCGCGGCTAG

Upstream 100 bases:

>100_bases
CGCTGGCGCCTGAAGTCGAAGAGGATTGGGGCGAATAGGAATAGTTGTTCAGTGCGGAGTCGTCGTCCACGACTCCGCTC
ACTGCCAGGGAGAAACCATT

Downstream 100 bases:

>100_bases
CGTACCACACAGGATAGATTTCAGCACAGACGAAGAGCGAATGTCTGCTGACGGCCAGACTCCGACGGATTACAACCTGT
TTGCAGGACTACCACCCAGC

Product: lysine biosynthesis enzyme LysX

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRIAVLCSRIRAEEKLLFQELERRGLDYVKVDDREQIFDLHTTTYPFDVVLERCIQHSRALYMLKIFNDAGVPTVNTYHV
ALTCGDKFLTTQALIHAGVPTPRCLLAFTPESALEAMEQLGYPVVLKPVIGSWGRMVSKINDRDAAEAILEHRDVLGNYQ
HSIFYIQQYIHKPGRDIRSFVIGDECIAAIYRTSPHWITNTARGGVATNCPVTPELADISVRAAHAVGGGVVAIDLLETP
EGELLVNEVNYTMEFRNSIDTTGVDIPARIIDYVLEVGRGRG

Sequences:

>Translated_282_residues
MRIAVLCSRIRAEEKLLFQELERRGLDYVKVDDREQIFDLHTTTYPFDVVLERCIQHSRALYMLKIFNDAGVPTVNTYHV
ALTCGDKFLTTQALIHAGVPTPRCLLAFTPESALEAMEQLGYPVVLKPVIGSWGRMVSKINDRDAAEAILEHRDVLGNYQ
HSIFYIQQYIHKPGRDIRSFVIGDECIAAIYRTSPHWITNTARGGVATNCPVTPELADISVRAAHAVGGGVVAIDLLETP
EGELLVNEVNYTMEFRNSIDTTGVDIPARIIDYVLEVGRGRG
>Mature_282_residues
MRIAVLCSRIRAEEKLLFQELERRGLDYVKVDDREQIFDLHTTTYPFDVVLERCIQHSRALYMLKIFNDAGVPTVNTYHV
ALTCGDKFLTTQALIHAGVPTPRCLLAFTPESALEAMEQLGYPVVLKPVIGSWGRMVSKINDRDAAEAILEHRDVLGNYQ
HSIFYIQQYIHKPGRDIRSFVIGDECIAAIYRTSPHWITNTARGGVATNCPVTPELADISVRAAHAVGGGVVAIDLLETP
EGELLVNEVNYTMEFRNSIDTTGVDIPARIIDYVLEVGRGRG

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of 'Glu-54' of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=246, Percent_Identity=29.2682926829268, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1787076, Length=235, Percent_Identity=30.2127659574468, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31498; Mature: 31498

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVLCSRIRAEEKLLFQELERRGLDYVKVDDREQIFDLHTTTYPFDVVLERCIQHSRA
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHEECCCCCCCHHHHHHHHHHHCCC
LYMLKIFNDAGVPTVNTYHVALTCGDKFLTTQALIHAGVPTPRCLLAFTPESALEAMEQL
EEEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHC
GYPVVLKPVIGSWGRMVSKINDRDAAEAILEHRDVLGNYQHSIFYIQQYIHKPGRDIRSF
CCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHH
VIGDECIAAIYRTSPHWITNTARGGVATNCPVTPELADISVRAAHAVGGGVVAIDLLETP
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHCCEEEEHHHCCCCEEEEEEEECC
EGELLVNEVNYTMEFRNSIDTTGVDIPARIIDYVLEVGRGRG
CCCEEEEECCEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRIAVLCSRIRAEEKLLFQELERRGLDYVKVDDREQIFDLHTTTYPFDVVLERCIQHSRA
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHEECCCCCCCHHHHHHHHHHHCCC
LYMLKIFNDAGVPTVNTYHVALTCGDKFLTTQALIHAGVPTPRCLLAFTPESALEAMEQL
EEEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHC
GYPVVLKPVIGSWGRMVSKINDRDAAEAILEHRDVLGNYQHSIFYIQQYIHKPGRDIRSF
CCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHH
VIGDECIAAIYRTSPHWITNTARGGVATNCPVTPELADISVRAAHAVGGGVVAIDLLETP
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHCCEEEEHHHCCCCEEEEEEEECC
EGELLVNEVNYTMEFRNSIDTTGVDIPARIIDYVLEVGRGRG
CCCEEEEECCEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12963379 [H]