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The map label for this gene is gap3 [H]

Identifier: 222523313

GI number: 222523313

Start: 12919

End: 13926

Strand: Direct

Name: gap3 [H]

Synonym: Chy400_0011

Alternate gene names: 222523313

Gene position: 12919-13926 (Clockwise)

Preceding gene: 222523305

Following gene: 222523314

Centisome position: 0.25

GC content: 57.64

Gene sequence:

>1008_bases
ATGGCTGTGCGCGTAGGGATCAACGGATTCGGGCGGATTGGACGGCTCGCGCTGCGGGCGGCGTGGGCATGGCCCGAACT
CGATATCGTCCATATTAACGAGGTTGGCGGTGATGCCGCTACCGCAGCGCATCTCCTCAGCTTTGATTCGACGCAGGGGC
GATGGTCGGTTGAGGTGCACGGTGAAGGCAATCAGTTGCTGATCGATGACAAAACGGTTGGTTACAGTCAGATTCGCGAT
CCCGGTGCTGTGCCATGGGCAGATGCCGGCGTTGACATTGTGCTTGAAGCAACCGGCAAGTTCCGCACTGCGGAGCAACT
GGCGGCCTATTTCAGCGCTGGGGTGAAGAAGGTGATTGTGGCGGCGCCGGTAAAGGGTGAGGCGCTGAATGTGGTTATGG
GGGTGAACGATCACTGGTATGAGCCAGATCGCCATCACCTGCTGACGGCCGCCTCGTGTACGACCAACTGTCTGGCACCG
ATTGTGAAAGTAATCCACGAAGGGATTGGCATTCGCCATGGCATGATCACAACCATTCACAGCTCGACTAACACCCAAAC
CGTGCACGACCGTCCACACAAAGATTTGCGACGGGCACGAGCGGCTAGTCTGTCACTCATCCCCACTACGACCGGCTCGG
CAACCGCTATCGGTCTGATCTTTCCTGAATTGCAGGGTAAACTCGACGGACAGGCGGTGCGGGTACCGTTGCTGAACGCA
TCGCTCACCGATTGCGTCTTCGAGGTACGTCGTCCGACAACTGTTGCCGAGGTGAACGGATTGTTACAGGCAGCGGCAGA
GGGTGCGCTGAAGGGGATTCTCGCCTATGAGACGCGCCCGCTGGTGTCGATTGACTTCCTCGGCAACCCACATTCGGCGA
TTGTCGACTCGCTGTGCACGATGGTCACCAACGAGACGCAGGTGAAGATTTACGCCTGGTACGACAATGAATGGGGATAC
GCCAATCGGTATGTGGAATTAGCGCGAAAGGTTGCCCTGCTCTTATGA

Upstream 100 bases:

>100_bases
TCTATCAGGCACGTACAGGCGTACAAATATCCCCTTGTCAGATGTCCCGGCATCCCGTACAATTACATCAATTCAGAATG
ATATAAATCAGGAGCATATC

Downstream 100 bases:

>100_bases
AAACAACGCCTTCCACTGAGGCATCGGCGACCATGACCCAACAGCGCGCCGACCGCCGTAACTATGTGCTGGTAACAATC
GCCTACTGGGCCGATACGCT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAVRVGINGFGRIGRLALRAAWAWPELDIVHINEVGGDAATAAHLLSFDSTQGRWSVEVHGEGNQLLIDDKTVGYSQIRD
PGAVPWADAGVDIVLEATGKFRTAEQLAAYFSAGVKKVIVAAPVKGEALNVVMGVNDHWYEPDRHHLLTAASCTTNCLAP
IVKVIHEGIGIRHGMITTIHSSTNTQTVHDRPHKDLRRARAASLSLIPTTTGSATAIGLIFPELQGKLDGQAVRVPLLNA
SLTDCVFEVRRPTTVAEVNGLLQAAAEGALKGILAYETRPLVSIDFLGNPHSAIVDSLCTMVTNETQVKIYAWYDNEWGY
ANRYVELARKVALLL

Sequences:

>Translated_335_residues
MAVRVGINGFGRIGRLALRAAWAWPELDIVHINEVGGDAATAAHLLSFDSTQGRWSVEVHGEGNQLLIDDKTVGYSQIRD
PGAVPWADAGVDIVLEATGKFRTAEQLAAYFSAGVKKVIVAAPVKGEALNVVMGVNDHWYEPDRHHLLTAASCTTNCLAP
IVKVIHEGIGIRHGMITTIHSSTNTQTVHDRPHKDLRRARAASLSLIPTTTGSATAIGLIFPELQGKLDGQAVRVPLLNA
SLTDCVFEVRRPTTVAEVNGLLQAAAEGALKGILAYETRPLVSIDFLGNPHSAIVDSLCTMVTNETQVKIYAWYDNEWGY
ANRYVELARKVALLL
>Mature_334_residues
AVRVGINGFGRIGRLALRAAWAWPELDIVHINEVGGDAATAAHLLSFDSTQGRWSVEVHGEGNQLLIDDKTVGYSQIRDP
GAVPWADAGVDIVLEATGKFRTAEQLAAYFSAGVKKVIVAAPVKGEALNVVMGVNDHWYEPDRHHLLTAASCTTNCLAPI
VKVIHEGIGIRHGMITTIHSSTNTQTVHDRPHKDLRRARAASLSLIPTTTGSATAIGLIFPELQGKLDGQAVRVPLLNAS
LTDCVFEVRRPTTVAEVNGLLQAAAEGALKGILAYETRPLVSIDFLGNPHSAIVDSLCTMVTNETQVKIYAWYDNEWGYA
NRYVELARKVALLL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=331, Percent_Identity=42.2960725075529, Blast_Score=271, Evalue=5e-73,
Organism=Homo sapiens, GI7657116, Length=326, Percent_Identity=41.1042944785276, Blast_Score=262, Evalue=4e-70,
Organism=Escherichia coli, GI1788079, Length=328, Percent_Identity=41.7682926829268, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1789295, Length=330, Percent_Identity=43.6363636363636, Blast_Score=268, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17534679, Length=335, Percent_Identity=40.8955223880597, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17534677, Length=335, Percent_Identity=40.8955223880597, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI32566163, Length=334, Percent_Identity=41.6167664670659, Blast_Score=264, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17568413, Length=334, Percent_Identity=41.6167664670659, Blast_Score=264, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6321631, Length=331, Percent_Identity=44.1087613293051, Blast_Score=291, Evalue=8e-80,
Organism=Saccharomyces cerevisiae, GI6322468, Length=331, Percent_Identity=42.9003021148036, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6322409, Length=331, Percent_Identity=41.3897280966767, Blast_Score=275, Evalue=8e-75,
Organism=Drosophila melanogaster, GI17933600, Length=327, Percent_Identity=41.2844036697248, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI18110149, Length=327, Percent_Identity=41.2844036697248, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI85725000, Length=327, Percent_Identity=40.9785932721713, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI22023983, Length=327, Percent_Identity=40.9785932721713, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19922412, Length=324, Percent_Identity=37.6543209876543, Blast_Score=247, Evalue=7e-66,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36196; Mature: 36065

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRVGINGFGRIGRLALRAAWAWPELDIVHINEVGGDAATAAHLLSFDSTQGRWSVEVH
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEE
GEGNQLLIDDKTVGYSQIRDPGAVPWADAGVDIVLEATGKFRTAEQLAAYFSAGVKKVIV
CCCCEEEEECCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEE
AAPVKGEALNVVMGVNDHWYEPDRHHLLTAASCTTNCLAPIVKVIHEGIGIRHGMITTIH
ECCCCCCEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
SSTNTQTVHDRPHKDLRRARAASLSLIPTTTGSATAIGLIFPELQGKLDGQAVRVPLLNA
CCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCEEEEEEEHHHCCCCCCCEEEEEEECC
SLTDCVFEVRRPTTVAEVNGLLQAAAEGALKGILAYETRPLVSIDFLGNPHSAIVDSLCT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHH
MVTNETQVKIYAWYDNEWGYANRYVELARKVALLL
HHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVRVGINGFGRIGRLALRAAWAWPELDIVHINEVGGDAATAAHLLSFDSTQGRWSVEVH
EEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEE
GEGNQLLIDDKTVGYSQIRDPGAVPWADAGVDIVLEATGKFRTAEQLAAYFSAGVKKVIV
CCCCEEEEECCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEE
AAPVKGEALNVVMGVNDHWYEPDRHHLLTAASCTTNCLAPIVKVIHEGIGIRHGMITTIH
ECCCCCCEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
SSTNTQTVHDRPHKDLRRARAASLSLIPTTTGSATAIGLIFPELQGKLDGQAVRVPLLNA
CCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCEEEEEEEHHHCCCCCCCEEEEEEECC
SLTDCVFEVRRPTTVAEVNGLLQAAAEGALKGILAYETRPLVSIDFLGNPHSAIVDSLCT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHH
MVTNETQVKIYAWYDNEWGYANRYVELARKVALLL
HHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]