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The map label for this gene is cls2 [H]

Identifier: 222151986

GI number: 222151986

Start: 1883327

End: 1884805

Strand: Direct

Name: cls2 [H]

Synonym: MCCL_1743

Alternate gene names: 222151986

Gene position: 1883327-1884805 (Clockwise)

Preceding gene: 222151985

Following gene: 222151987

Centisome position: 89.58

GC content: 37.12

Gene sequence:

>1479_bases
ATGTTGGAGCAGTTATCACAAATTGATTACTCACAAATTTTCTCAATTTTATTTTTTGTAGGATTTATCTTTAACATCTT
TTTAGCTTTCGTCATCATCTTTTTTGAACGAAGACAAGCAGGCTCTACGTGGGCATGGCTACTCGTACTATTTTTCTTAC
CTATCGTCGGATTCATACTTTATCTATTGTTCGGTCGTCAAATCAATCATCAGACTATATTCACTTTAAATGATGAGGAC
CGGTTAGACCTAGAACTTATTGTCAGTGCTCAACGTAAAGCGATAGACAATGGTAATATAGAAGTGCGCTCTGGAGAAAT
TATTAAACATAGACATATCATCAGAATGTTGCTGCACAACTATGCCTCTTTTCTGACTACCGATAATAAGGTGGAGATTT
TAACGGATGGCAGAAAGAAATTTGACGCTTTATTAAATGATATTCATAACGCAACAGATCATATTCATATCCAGTACTAT
ATCTTCAAAAAGGATGGTATCGGTCTAGAGATCCTCAATGCACTGATGAAGAAACTTGAAGAAGGTGTAGAAGTGAAGAT
GCTATATGATGATATCGGTTCAAGAACTTTGTCTCTCTCTAGATTCAAGCAGTTTAAAAAACTAGGTGGACAAGTAGAAT
CCTTCTTTCCTTCTAAATTACCGCTTATCAACTTTAGAATGAATAATCGAAATCACCGCAAGATTGTCGTAATCGACGGT
AAAATTGGCTATATCGGCGGTTTTAATGTAGGTGATGAATACCTGGGTCTGGATAAGAAATTTGGTTACTGGCGCGATAC
GCATCTCAGAGTAGAAGGCGATGCAGTAAATGCCTTACAGCTCCGTTTTATGATGGACTGGAATTCACAGATTACACGTG
ACTTCATGTCTTATGATAAGAAGTACTTTCCAGATGTAACAAGCAAAGGTGATATCGGCATTCAGATTGTATCAAGCGGA
CCGGATTCCTCTGCTCAACATATAAAAAACGGATATCTTAAAATGATCACTTCCGCAAAAAAATCTATATATATCCAGTC
TCCTTATTTCATCCCTGATACATCATTACTAGATGCATTAAAAATTGCCGCAATGACTGGTGTAGAAGTTAATATCATGA
TTCCGAATAAACCTGACCATCCGTTTGTATATTGGGCTACATATTCAAACGTCGGTGAACTTCTCGATGTCGGTTGTAAT
ATCTTCATTTATGAGAATGGCTTCATTCACACAAAGATGCTCCTCATCGATGACGAAGTTGCAAGCGTCGGAACTGCCAA
TATGGATTTTAGAAGCTTTGAATTAAATTTTGAAGTGAATGCCTTTATATACGATGATGTCGTAGCAAAAAAATTACGAA
AATCATTTGAAGACGATGTGCACGTCTCTTCGCAGCTTACTAAGGAAATTTATGATCAGCGTGTCCTCCTTATTCGTGTG
AAAGAAGCCATTGCGCGACTGCTTTCTCCAATATTATAG

Upstream 100 bases:

>100_bases
TTTATCGATAACTTTATGAATTATTTGTTAAATATTACATCGGTCTCTACATACCGTGCTTCTATCTATTCATGTATAAT
AGATTTAATTAGGAGGGGTT

Downstream 100 bases:

>100_bases
GGGCAGTTCGAGCTCTACCTCTATTATATAAATCTAAGCTGGCGTATACATAAAATCTAGAGCAATGTTTGCAAGGATTA
AGCTATGTACAACAAGATCA

Product: hypothetical protein

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MLEQLSQIDYSQIFSILFFVGFIFNIFLAFVIIFFERRQAGSTWAWLLVLFFLPIVGFILYLLFGRQINHQTIFTLNDED
RLDLELIVSAQRKAIDNGNIEVRSGEIIKHRHIIRMLLHNYASFLTTDNKVEILTDGRKKFDALLNDIHNATDHIHIQYY
IFKKDGIGLEILNALMKKLEEGVEVKMLYDDIGSRTLSLSRFKQFKKLGGQVESFFPSKLPLINFRMNNRNHRKIVVIDG
KIGYIGGFNVGDEYLGLDKKFGYWRDTHLRVEGDAVNALQLRFMMDWNSQITRDFMSYDKKYFPDVTSKGDIGIQIVSSG
PDSSAQHIKNGYLKMITSAKKSIYIQSPYFIPDTSLLDALKIAAMTGVEVNIMIPNKPDHPFVYWATYSNVGELLDVGCN
IFIYENGFIHTKMLLIDDEVASVGTANMDFRSFELNFEVNAFIYDDVVAKKLRKSFEDDVHVSSQLTKEIYDQRVLLIRV
KEAIARLLSPIL

Sequences:

>Translated_492_residues
MLEQLSQIDYSQIFSILFFVGFIFNIFLAFVIIFFERRQAGSTWAWLLVLFFLPIVGFILYLLFGRQINHQTIFTLNDED
RLDLELIVSAQRKAIDNGNIEVRSGEIIKHRHIIRMLLHNYASFLTTDNKVEILTDGRKKFDALLNDIHNATDHIHIQYY
IFKKDGIGLEILNALMKKLEEGVEVKMLYDDIGSRTLSLSRFKQFKKLGGQVESFFPSKLPLINFRMNNRNHRKIVVIDG
KIGYIGGFNVGDEYLGLDKKFGYWRDTHLRVEGDAVNALQLRFMMDWNSQITRDFMSYDKKYFPDVTSKGDIGIQIVSSG
PDSSAQHIKNGYLKMITSAKKSIYIQSPYFIPDTSLLDALKIAAMTGVEVNIMIPNKPDHPFVYWATYSNVGELLDVGCN
IFIYENGFIHTKMLLIDDEVASVGTANMDFRSFELNFEVNAFIYDDVVAKKLRKSFEDDVHVSSQLTKEIYDQRVLLIRV
KEAIARLLSPIL
>Mature_492_residues
MLEQLSQIDYSQIFSILFFVGFIFNIFLAFVIIFFERRQAGSTWAWLLVLFFLPIVGFILYLLFGRQINHQTIFTLNDED
RLDLELIVSAQRKAIDNGNIEVRSGEIIKHRHIIRMLLHNYASFLTTDNKVEILTDGRKKFDALLNDIHNATDHIHIQYY
IFKKDGIGLEILNALMKKLEEGVEVKMLYDDIGSRTLSLSRFKQFKKLGGQVESFFPSKLPLINFRMNNRNHRKIVVIDG
KIGYIGGFNVGDEYLGLDKKFGYWRDTHLRVEGDAVNALQLRFMMDWNSQITRDFMSYDKKYFPDVTSKGDIGIQIVSSG
PDSSAQHIKNGYLKMITSAKKSIYIQSPYFIPDTSLLDALKIAAMTGVEVNIMIPNKPDHPFVYWATYSNVGELLDVGCN
IFIYENGFIHTKMLLIDDEVASVGTANMDFRSFELNFEVNAFIYDDVVAKKLRKSFEDDVHVSSQLTKEIYDQRVLLIRV
KEAIARLLSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=497, Percent_Identity=28.7726358148893, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1787007, Length=349, Percent_Identity=29.7994269340974, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 56708; Mature: 56708

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQLSQIDYSQIFSILFFVGFIFNIFLAFVIIFFERRQAGSTWAWLLVLFFLPIVGFIL
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
YLLFGRQINHQTIFTLNDEDRLDLELIVSAQRKAIDNGNIEVRSGEIIKHRHIIRMLLHN
HHHHCCCCCCCEEEEECCCCCCCHHEEEHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
YASFLTTDNKVEILTDGRKKFDALLNDIHNATDHIHIQYYIFKKDGIGLEILNALMKKLE
HHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHH
EGVEVKMLYDDIGSRTLSLSRFKQFKKLGGQVESFFPSKLPLINFRMNNRNHRKIVVIDG
CCCEEEEEEHHCCCCEEEHHHHHHHHHHCCHHHHCCCCCCCEEEEEECCCCCCEEEEEEC
KIGYIGGFNVGDEYLGLDKKFGYWRDTHLRVEGDAVNALQLRFMMDWNSQITRDFMSYDK
CEEEEECCCCCHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCH
KYFPDVTSKGDIGIQIVSSGPDSSAQHIKNGYLKMITSAKKSIYIQSPYFIPDTSLLDAL
HHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCEECCCHHHHHHH
KIAAMTGVEVNIMIPNKPDHPFVYWATYSNVGELLDVGCNIFIYENGFIHTKMLLIDDEV
HHHHCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCEEEEEEEEECHH
ASVGTANMDFRSFELNFEVNAFIYDDVVAKKLRKSFEDDVHVSSQLTKEIYDQRVLLIRV
HHCCCCCCCEEEEEEEEEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEHHH
KEAIARLLSPIL
HHHHHHHHHCCC
>Mature Secondary Structure
MLEQLSQIDYSQIFSILFFVGFIFNIFLAFVIIFFERRQAGSTWAWLLVLFFLPIVGFIL
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
YLLFGRQINHQTIFTLNDEDRLDLELIVSAQRKAIDNGNIEVRSGEIIKHRHIIRMLLHN
HHHHCCCCCCCEEEEECCCCCCCHHEEEHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
YASFLTTDNKVEILTDGRKKFDALLNDIHNATDHIHIQYYIFKKDGIGLEILNALMKKLE
HHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHH
EGVEVKMLYDDIGSRTLSLSRFKQFKKLGGQVESFFPSKLPLINFRMNNRNHRKIVVIDG
CCCEEEEEEHHCCCCEEEHHHHHHHHHHCCHHHHCCCCCCCEEEEEECCCCCCEEEEEEC
KIGYIGGFNVGDEYLGLDKKFGYWRDTHLRVEGDAVNALQLRFMMDWNSQITRDFMSYDK
CEEEEECCCCCHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCH
KYFPDVTSKGDIGIQIVSSGPDSSAQHIKNGYLKMITSAKKSIYIQSPYFIPDTSLLDAL
HHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCEECCCHHHHHHH
KIAAMTGVEVNIMIPNKPDHPFVYWATYSNVGELLDVGCNIFIYENGFIHTKMLLIDDEV
HHHHCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCEEEEEEEEECHH
ASVGTANMDFRSFELNFEVNAFIYDDVVAKKLRKSFEDDVHVSSQLTKEIYDQRVLLIRV
HHCCCCCCCEEEEEEEEEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEHHH
KEAIARLLSPIL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA