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The map label for this gene is hsdM [H]

Identifier: 222151724

GI number: 222151724

Start: 1598602

End: 1599843

Strand: Direct

Name: hsdM [H]

Synonym: MCCL_1477

Alternate gene names: 222151724

Gene position: 1598602-1599843 (Clockwise)

Preceding gene: 222151721

Following gene: 222151725

Centisome position: 76.04

GC content: 36.47

Gene sequence:

>1242_bases
ATGGAGAAGATAAAAGTAGCGGAAATGTTTGCAGGTGTCGGTGGTTTTCGTATCGGTCTAGAAAACACTAACAATAATAT
GTTTGAAGTTACTTGGGCAAACCAATGGGAACCTTCTAGAAAAGTGCAGCATGCTTTTGATTGTTATTCACGAAACTTCA
AAACAGGCATTCATAGTAATCAGGATATTACAGAAGTTCCAAATGCTGAACTTGCTGCGACGAATGTCGACATGATTGTT
GGTGGTTTTCCTTGTCAAGACTATTCAGTAGCACGCTCATTAAATGGCGAACTCGGCATGCAAGGAAAGAAAGGTGTATT
ATTCTGGGAAATTACACGATTCATACAAAACGTTGCACCTAAATATATTCTACTTGAAAACGTTGATAGATTATTAAAAT
CTCCTTCTAAACAAAGAGGAAGAGATTTCGGAGTAATGCTTTCTACATTAAACGAACTTGGTTATGATGTTGAATGGCGT
GTTATCAATGCAGCAGACTATGGTAATGCACAACGTAGAAGACGTGTATTCATCTTTGGATATAGACGCGAAACGAACTA
TGCTAAATCAATACTCAAGAAAACACAGGAACAAATTGTATTTAAAGATGGATTCTTTGCGAAAGCATTCCCTATCACTG
ATGAAATAGTCAAGAATCGTGTTTCTACTACTTCACTTTCGAGTGATATTGTTAGTGTTTCTGATAATTTTTCTTATACA
TTTCATCCGTCGGGCTATATGAAGAATGGAGAAGTTTATACAGCTCATACTGTACCGAAATATGAATCTCCAGCTACACT
TGGATCTGTCCTCGTTGAAGAAGTCCCAGAACAATATTACCTTCCAGAAGATAAAGTTGAGAAGTTCAAATATTTAAGAG
GCTCAAAGAAAATCGAAAGAATTACAGCAGATGGTCATAAGTATATTTATAGTGAAGGTGGTATGTCAGAAAGCGATGAC
ATTAATTTACCTGCAAGAACGATGCTAACGAGTGAATCCTCAGTAAATAGAAGCACACATTTTATCAAAGAGTCAGAAGA
TAAATTTAGAACACTTACCCCTGTTGAAGCTGAGCGATTGAACGGTTTTCCTGATAACTGGACTGAAGGTATGCCAGAGA
AGATGAGATTCTTCTGTATGGGGAACGCATTAGTTGTACCATTAATTACGAGAATGGGAAATCAAATTGAGGAAATCGAA
AAACTCTATCCAGATGATATTACGCAATTAAATCTTTTTTAG

Upstream 100 bases:

>100_bases
ATCGTTCGTATAATTAAGATGGTAATAAATTTGATATATCAATCAATCTAGATCTTTGATAAAATATAAAAGTTAATCAT
AATTTATAAGGAGGCCCACT

Downstream 100 bases:

>100_bases
TCTACCAATTGATGGTAGACTTTTTTATTTGAGAAAAATATACTTGTAATAAGTAACCTCGATAGGAGATGATTAATATG
AGTAATTTTAATTTGGATAT

Product: type II modification methyltransferase

Products: NA

Alternate protein names: M.Sau3AI; Cytosine-specific methyltransferase Sau3AI [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MEKIKVAEMFAGVGGFRIGLENTNNNMFEVTWANQWEPSRKVQHAFDCYSRNFKTGIHSNQDITEVPNAELAATNVDMIV
GGFPCQDYSVARSLNGELGMQGKKGVLFWEITRFIQNVAPKYILLENVDRLLKSPSKQRGRDFGVMLSTLNELGYDVEWR
VINAADYGNAQRRRRVFIFGYRRETNYAKSILKKTQEQIVFKDGFFAKAFPITDEIVKNRVSTTSLSSDIVSVSDNFSYT
FHPSGYMKNGEVYTAHTVPKYESPATLGSVLVEEVPEQYYLPEDKVEKFKYLRGSKKIERITADGHKYIYSEGGMSESDD
INLPARTMLTSESSVNRSTHFIKESEDKFRTLTPVEAERLNGFPDNWTEGMPEKMRFFCMGNALVVPLITRMGNQIEEIE
KLYPDDITQLNLF

Sequences:

>Translated_413_residues
MEKIKVAEMFAGVGGFRIGLENTNNNMFEVTWANQWEPSRKVQHAFDCYSRNFKTGIHSNQDITEVPNAELAATNVDMIV
GGFPCQDYSVARSLNGELGMQGKKGVLFWEITRFIQNVAPKYILLENVDRLLKSPSKQRGRDFGVMLSTLNELGYDVEWR
VINAADYGNAQRRRRVFIFGYRRETNYAKSILKKTQEQIVFKDGFFAKAFPITDEIVKNRVSTTSLSSDIVSVSDNFSYT
FHPSGYMKNGEVYTAHTVPKYESPATLGSVLVEEVPEQYYLPEDKVEKFKYLRGSKKIERITADGHKYIYSEGGMSESDD
INLPARTMLTSESSVNRSTHFIKESEDKFRTLTPVEAERLNGFPDNWTEGMPEKMRFFCMGNALVVPLITRMGNQIEEIE
KLYPDDITQLNLF
>Mature_413_residues
MEKIKVAEMFAGVGGFRIGLENTNNNMFEVTWANQWEPSRKVQHAFDCYSRNFKTGIHSNQDITEVPNAELAATNVDMIV
GGFPCQDYSVARSLNGELGMQGKKGVLFWEITRFIQNVAPKYILLENVDRLLKSPSKQRGRDFGVMLSTLNELGYDVEWR
VINAADYGNAQRRRRVFIFGYRRETNYAKSILKKTQEQIVFKDGFFAKAFPITDEIVKNRVSTTSLSSDIVSVSDNFSYT
FHPSGYMKNGEVYTAHTVPKYESPATLGSVLVEEVPEQYYLPEDKVEKFKYLRGSKKIERITADGHKYIYSEGGMSESDD
INLPARTMLTSESSVNRSTHFIKESEDKFRTLTPVEAERLNGFPDNWTEGMPEKMRFFCMGNALVVPLITRMGNQIEEIE
KLYPDDITQLNLF

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on C-4 on both strands and protects the DNA from cleavage by the Sau3AI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=205, Percent_Identity=31.219512195122, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 47208; Mature: 47208

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVAEMFAGVGGFRIGLENTNNNMFEVTWANQWEPSRKVQHAFDCYSRNFKTGIHSN
CCCCHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCC
QDITEVPNAELAATNVDMIVGGFPCQDYSVARSLNGELGMQGKKGVLFWEITRFIQNVAP
CCCCCCCCCCEEECCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
KYILLENVDRLLKSPSKQRGRDFGVMLSTLNELGYDVEWRVINAADYGNAQRRRRVFIFG
CEEHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHCCEEEEEE
YRRETNYAKSILKKTQEQIVFKDGFFAKAFPITDEIVKNRVSTTSLSSDIVSVSDNFSYT
EECCCHHHHHHHHHHHHHEEEECCCCEEECCCCHHHHHHHHHHHHHCCCEEEECCCCEEE
FHPSGYMKNGEVYTAHTVPKYESPATLGSVLVEEVPEQYYLPEDKVEKFKYLRGSKKIER
ECCCCCEECCEEEEEECCCCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCHHHH
ITADGHKYIYSEGGMSESDDINLPARTMLTSESSVNRSTHFIKESEDKFRTLTPVEAERL
EECCCCEEEEECCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHCCCCHHHCCCCCCHHHC
NGFPDNWTEGMPEKMRFFCMGNALVVPLITRMGNQIEEIEKLYPDDITQLNLF
CCCCCCHHCCCCHHHEEEEECCHHHHHHHHHHCHHHHHHHHHCCCCCCEECCC
>Mature Secondary Structure
MEKIKVAEMFAGVGGFRIGLENTNNNMFEVTWANQWEPSRKVQHAFDCYSRNFKTGIHSN
CCCCHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCC
QDITEVPNAELAATNVDMIVGGFPCQDYSVARSLNGELGMQGKKGVLFWEITRFIQNVAP
CCCCCCCCCCEEECCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
KYILLENVDRLLKSPSKQRGRDFGVMLSTLNELGYDVEWRVINAADYGNAQRRRRVFIFG
CEEHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHCCEEEEEE
YRRETNYAKSILKKTQEQIVFKDGFFAKAFPITDEIVKNRVSTTSLSSDIVSVSDNFSYT
EECCCHHHHHHHHHHHHHEEEECCCCEEECCCCHHHHHHHHHHHHHCCCEEEECCCCEEE
FHPSGYMKNGEVYTAHTVPKYESPATLGSVLVEEVPEQYYLPEDKVEKFKYLRGSKKIER
ECCCCCEECCEEEEEECCCCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCHHHH
ITADGHKYIYSEGGMSESDDINLPARTMLTSESSVNRSTHFIKESEDKFRTLTPVEAERL
EECCCCEEEEECCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHCCCCHHHCCCCCCHHHC
NGFPDNWTEGMPEKMRFFCMGNALVVPLITRMGNQIEEIEKLYPDDITQLNLF
CCCCCCHHCCCCHHHEEEEECCHHHHHHHHHHCHHHHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2227451 [H]