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The map label for this gene is ansA [H]

Identifier: 222151376

GI number: 222151376

Start: 1237484

End: 1238449

Strand: Direct

Name: ansA [H]

Synonym: MCCL_1129

Alternate gene names: 222151376

Gene position: 1237484-1238449 (Clockwise)

Preceding gene: 222151355

Following gene: 222151392

Centisome position: 58.86

GC content: 39.23

Gene sequence:

>966_bases
ATGAAAAATATTCTCGTAATGCATACAGGTGGAACAATCAGTATGTCTGAAAATGCACAAGGTGTCGTCACAACGAATGA
CACAAACCCTATGTCAAATATCGAAATCATTCAGTCGATTGCAAACATTACCGAAAAACACCCATTTCAAATCCCTTCCC
CCCATATGACGCTTAAACATATGAATATATTAAGAAAATCAATTATCAATGAAATATATGAAGAGAACTATGATGGTATC
GTCATCACACATGGCACTGACACGCTCGAAGAAACAGCATATTTTCTAGATTTGACTCTCAACGTTTCAATTCCAGTAAT
CTTAACCGGTGCAATGCGTTCATCCAACGAAATCGGGGCAGATGGTCTGTATAACTACATCTCCGCAATACGTACAGCTT
GCCATGATGATGCCAAAAATAAAGGCGTACTTGTTGTGTTTAATGACGAAATTCATACAGCTATGAACGTAACAAAGACC
CATACATCAAATACAAGCACTTTTCAAAGTCCGAACTACGGACCACTCGGTATTATCACGAAAAACAATATTTATTTCCA
TCATCATCCATTGACACATCCCCCGCTTGTTAAAGTCAATGAAGCAATTAAAGTCGGACTCGTTAAAGCCCATAGCGACC
TGGATTCAAACTTAATCAAATTCTTCGCTGATCAAGAATATGACGGGCTTGTTATAGAGGCGCTTGGACAAGGAAACTTG
CCGCCTTCTTCATTAGACGGCATTCAATATCTAATCGATAAAAATATCCCGATTGTCATCGTATCCCGATGTTTCAATGG
TATTGCAAGTGGTAACTATCAATACGAAGGCGGGGGATATGAGCTACAGAAGATGGGACTCATATTCTCCAACGGACTTA
ACGGCCAGAAAGCGCGTCTAAAACTCATCGTTGCAATAAGTAATGGTTTAAACGACGCTCTTCTTGATGATTACTTCAAA
CGTTAA

Upstream 100 bases:

>100_bases
TCTATTGCGATATTTATCTTATTCATTTATATACCTACCTTGTTGTTTAATCTTAGTTATTTTATCATAGAGCTATATCA
ATGTATATTGGAGGAGTAAA

Downstream 100 bases:

>100_bases
AAATACGTGAACAAATTATTGTCCACGCATTTCTTTTTTTATAATATCATCAGCTATCAGTCCGCCATGAAATTTTCCGT
TTTCAATGAATATCGTATTT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKNILVMHTGGTISMSENAQGVVTTNDTNPMSNIEIIQSIANITEKHPFQIPSPHMTLKHMNILRKSIINEIYEENYDGI
VITHGTDTLEETAYFLDLTLNVSIPVILTGAMRSSNEIGADGLYNYISAIRTACHDDAKNKGVLVVFNDEIHTAMNVTKT
HTSNTSTFQSPNYGPLGIITKNNIYFHHHPLTHPPLVKVNEAIKVGLVKAHSDLDSNLIKFFADQEYDGLVIEALGQGNL
PPSSLDGIQYLIDKNIPIVIVSRCFNGIASGNYQYEGGGYELQKMGLIFSNGLNGQKARLKLIVAISNGLNDALLDDYFK
R

Sequences:

>Translated_321_residues
MKNILVMHTGGTISMSENAQGVVTTNDTNPMSNIEIIQSIANITEKHPFQIPSPHMTLKHMNILRKSIINEIYEENYDGI
VITHGTDTLEETAYFLDLTLNVSIPVILTGAMRSSNEIGADGLYNYISAIRTACHDDAKNKGVLVVFNDEIHTAMNVTKT
HTSNTSTFQSPNYGPLGIITKNNIYFHHHPLTHPPLVKVNEAIKVGLVKAHSDLDSNLIKFFADQEYDGLVIEALGQGNL
PPSSLDGIQYLIDKNIPIVIVSRCFNGIASGNYQYEGGGYELQKMGLIFSNGLNGQKARLKLIVAISNGLNDALLDDYFK
R
>Mature_321_residues
MKNILVMHTGGTISMSENAQGVVTTNDTNPMSNIEIIQSIANITEKHPFQIPSPHMTLKHMNILRKSIINEIYEENYDGI
VITHGTDTLEETAYFLDLTLNVSIPVILTGAMRSSNEIGADGLYNYISAIRTACHDDAKNKGVLVVFNDEIHTAMNVTKT
HTSNTSTFQSPNYGPLGIITKNNIYFHHHPLTHPPLVKVNEAIKVGLVKAHSDLDSNLIKFFADQEYDGLVIEALGQGNL
PPSSLDGIQYLIDKNIPIVIVSRCFNGIASGNYQYEGGGYELQKMGLIFSNGLNGQKARLKLIVAISNGLNDALLDDYFK
R

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=307, Percent_Identity=25.4071661237785, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1789327, Length=285, Percent_Identity=36.4912280701754, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1788065, Length=291, Percent_Identity=28.8659793814433, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17558280, Length=340, Percent_Identity=26.4705882352941, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193207372, Length=339, Percent_Identity=26.5486725663717, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558278, Length=340, Percent_Identity=26.4705882352941, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193207374, Length=340, Percent_Identity=26.4705882352941, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=328, Percent_Identity=27.4390243902439, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323187, Length=328, Percent_Identity=27.4390243902439, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323186, Length=328, Percent_Identity=27.4390243902439, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323184, Length=328, Percent_Identity=27.4390243902439, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320527, Length=322, Percent_Identity=27.9503105590062, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24645530, Length=336, Percent_Identity=26.4880952380952, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24639609, Length=275, Percent_Identity=28, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35358; Mature: 35358

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILVMHTGGTISMSENAQGVVTTNDTNPMSNIEIIQSIANITEKHPFQIPSPHMTLKH
CCCEEEEECCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
MNILRKSIINEIYEENYDGIVITHGTDTLEETAYFLDLTLNVSIPVILTGAMRSSNEIGA
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCEEEEEEEEECEEEEEEEECCCCCCCCCC
DGLYNYISAIRTACHDDAKNKGVLVVFNDEIHTAMNVTKTHTSNTSTFQSPNYGPLGIIT
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEE
KNNIYFHHHPLTHPPLVKVNEAIKVGLVKAHSDLDSNLIKFFADQEYDGLVIEALGQGNL
ECCEEEEECCCCCCCCEEECCCEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCC
PPSSLDGIQYLIDKNIPIVIVSRCFNGIASGNYQYEGGGYELQKMGLIFSNGLNGQKARL
CCCHHCHHHHHHCCCCCEEEEEHHHHHHHCCCEEECCCCEEEEEEEEEEECCCCCCCEEE
KLIVAISNGLNDALLDDYFKR
EEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MKNILVMHTGGTISMSENAQGVVTTNDTNPMSNIEIIQSIANITEKHPFQIPSPHMTLKH
CCCEEEEECCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
MNILRKSIINEIYEENYDGIVITHGTDTLEETAYFLDLTLNVSIPVILTGAMRSSNEIGA
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCEEEEEEEEECEEEEEEEECCCCCCCCCC
DGLYNYISAIRTACHDDAKNKGVLVVFNDEIHTAMNVTKTHTSNTSTFQSPNYGPLGIIT
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEE
KNNIYFHHHPLTHPPLVKVNEAIKVGLVKAHSDLDSNLIKFFADQEYDGLVIEALGQGNL
ECCEEEEECCCCCCCCEEECCCEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCC
PPSSLDGIQYLIDKNIPIVIVSRCFNGIASGNYQYEGGGYELQKMGLIFSNGLNGQKARL
CCCHHCHHHHHHCCCCCEEEEEHHHHHHHCCCEEECCCCEEEEEEEEEEECCCCCCCEEE
KLIVAISNGLNDALLDDYFKR
EEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]