Click here to switch to the map view.

The map label for this gene is cwlA [H]

Identifier: 222151186

GI number: 222151186

Start: 1031369

End: 1032268

Strand: Direct

Name: cwlA [H]

Synonym: MCCL_0937

Alternate gene names: 222151186

Gene position: 1031369-1032268 (Clockwise)

Preceding gene: 222151185

Following gene: 222151192

Centisome position: 49.06

GC content: 37.0

Gene sequence:

>900_bases
TTGTCTTTTTTAAATACAAATTATAGGGAGAGAAATAACATGACATATAAAATTATTAATTCATGGTTACCAGCAAGCAA
ATATAGTTTAAAAGCACCTTTCTCAATGGATCCTGAATATATTACAGTTCACAATACAGGTAATACAGCGAGTGCTAGAG
AAGAAGCAGCGTATCATAACTCAAATAATAGCGAAACATCGTATCATGTAGTTATCGATGAAAATGAAGTACATCAATTA
ATTCCTTTCAGTCGTAACGCTTGGGATTCCGGAGATGGTAGAGGTAACGGAAACATGAAATCTATCGGAATCGAAATCGC
ACGTTCAATGGATAATGGATATAGTGGTCCTAAGTCACAACGTTATATGCAAGCAGAAGAAAACGCCGCGTTATATATCG
CTCACGTTATGCACGAAAGAGGTTGGGATATGAGCCGACTAAAACGACATTATGATTGGTCGGGTAAAGACTGCCCTCAC
AAAATGCACGCTACAGGCACATATCAACAATTTAGAGATAAAGTTCAAAAGCATCTTGTCGCTTTAAATAACGGTAAAAA
AGTTCAAGCAAGTACAGTTAAAAAACCGGTAAAGAAAGCACCTGCTAAAAATACGGGTGGTTGGCAAGTCAATCAGTACG
GTACGAAGTGGAAAAACGAAAAAGGTACATTTATTAATGGAAGTGAGCCTATTCAAGCCTATTACGTTGGACCATTTGTA
ATTGCTAAGAATAAAGCTGGGAAGTTACCTGCTAAAGCTACAGTTAAGTATGATGAAGTAATGGTGCAAGATGGATATGT
TTGGATCGCTTATGATGCGAATGATGGAAAGCGTATCTACTTACCAATCAGAACACATAAAAATGGCATTGATGGACCAC
TTTGGGGAACAATTAAATAA

Upstream 100 bases:

>100_bases
GCTAAACATTCTAAAGACTTGCCAGGTGGACAGGTTGACGTAAGTGTTAAGGTTCAGTCAGAAAAAAACGAAGAAATAAA
TTAAGGCAGCTCACTATGAG

Downstream 100 bases:

>100_bases
GCATGATTACTAGCCCTGCACTCAAATTAATGAGTGCAGGGCTTTTTTTATTTCGGTCATATACCCGAATAATTTAAAAT
TCGGTTAAATAGCCGGATAT

Product: hypothetical protein

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSFLNTNYRERNNMTYKIINSWLPASKYSLKAPFSMDPEYITVHNTGNTASAREEAAYHNSNNSETSYHVVIDENEVHQL
IPFSRNAWDSGDGRGNGNMKSIGIEIARSMDNGYSGPKSQRYMQAEENAALYIAHVMHERGWDMSRLKRHYDWSGKDCPH
KMHATGTYQQFRDKVQKHLVALNNGKKVQASTVKKPVKKAPAKNTGGWQVNQYGTKWKNEKGTFINGSEPIQAYYVGPFV
IAKNKAGKLPAKATVKYDEVMVQDGYVWIAYDANDGKRIYLPIRTHKNGIDGPLWGTIK

Sequences:

>Translated_299_residues
MSFLNTNYRERNNMTYKIINSWLPASKYSLKAPFSMDPEYITVHNTGNTASAREEAAYHNSNNSETSYHVVIDENEVHQL
IPFSRNAWDSGDGRGNGNMKSIGIEIARSMDNGYSGPKSQRYMQAEENAALYIAHVMHERGWDMSRLKRHYDWSGKDCPH
KMHATGTYQQFRDKVQKHLVALNNGKKVQASTVKKPVKKAPAKNTGGWQVNQYGTKWKNEKGTFINGSEPIQAYYVGPFV
IAKNKAGKLPAKATVKYDEVMVQDGYVWIAYDANDGKRIYLPIRTHKNGIDGPLWGTIK
>Mature_298_residues
SFLNTNYRERNNMTYKIINSWLPASKYSLKAPFSMDPEYITVHNTGNTASAREEAAYHNSNNSETSYHVVIDENEVHQLI
PFSRNAWDSGDGRGNGNMKSIGIEIARSMDNGYSGPKSQRYMQAEENAALYIAHVMHERGWDMSRLKRHYDWSGKDCPHK
MHATGTYQQFRDKVQKHLVALNNGKKVQASTVKKPVKKAPAKNTGGWQVNQYGTKWKNEKGTFINGSEPIQAYYVGPFVI
AKNKAGKLPAKATVKYDEVMVQDGYVWIAYDANDGKRIYLPIRTHKNGIDGPLWGTIK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 33915; Mature: 33783

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLNTNYRERNNMTYKIINSWLPASKYSLKAPFSMDPEYITVHNTGNTASAREEAAYHN
CCCCCCCCHHCCCCEEEEEECCCCCCCCEEECCCCCCCCEEEEECCCCCCHHHHHHHHCC
SNNSETSYHVVIDENEVHQLIPFSRNAWDSGDGRGNGNMKSIGIEIARSMDNGYSGPKSQ
CCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCHH
RYMQAEENAALYIAHVMHERGWDMSRLKRHYDWSGKDCPHKMHATGTYQQFRDKVQKHLV
HHEECCCCCEEEEEEEHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEE
ALNNGKKVQASTVKKPVKKAPAKNTGGWQVNQYGTKWKNEKGTFINGSEPIQAYYVGPFV
EECCCCEEEHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEECCCCCEEEEEECEEE
IAKNKAGKLPAKATVKYDEVMVQDGYVWIAYDANDGKRIYLPIRTHKNGIDGPLWGTIK
EECCCCCCCCCCEEEEEHEEEEECCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SFLNTNYRERNNMTYKIINSWLPASKYSLKAPFSMDPEYITVHNTGNTASAREEAAYHN
CCCCCCCHHCCCCEEEEEECCCCCCCCEEECCCCCCCCEEEEECCCCCCHHHHHHHHCC
SNNSETSYHVVIDENEVHQLIPFSRNAWDSGDGRGNGNMKSIGIEIARSMDNGYSGPKSQ
CCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCHH
RYMQAEENAALYIAHVMHERGWDMSRLKRHYDWSGKDCPHKMHATGTYQQFRDKVQKHLV
HHEECCCCCEEEEEEEHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEE
ALNNGKKVQASTVKKPVKKAPAKNTGGWQVNQYGTKWKNEKGTFINGSEPIQAYYVGPFV
EECCCCEEEHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEECCCCCEEEEEECEEE
IAKNKAGKLPAKATVKYDEVMVQDGYVWIAYDANDGKRIYLPIRTHKNGIDGPLWGTIK
EECCCCCCCCCCEEEEEHEEEEECCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2127796; 1683402; 7704261; 8969508; 9384377 [H]