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The map label for this gene is maiB [H]

Identifier: 222151135

GI number: 222151135

Start: 977330

End: 978859

Strand: Direct

Name: maiB [H]

Synonym: MCCL_0886

Alternate gene names: 222151135

Gene position: 977330-978859 (Clockwise)

Preceding gene: 222151134

Following gene: 222151136

Centisome position: 46.49

GC content: 39.08

Gene sequence:

>1530_bases
ATGAATGAAGAAGGTAGATTACAATCTTCAGGTGCAAAAGGTAACGCAGTACCGGAGAAAGATTATAGTAAATATTTTGA
ACATGTTTACCAGGGACCAAATTTAAAAGAGGCGAAACGTCGTGGTAAAGAAGACGTGAAATATCATAATGATTTCAAGA
TTGATGAGCAGTATCGCGGATTAGGTCGCGGTCGTAAATTTTATATCAGAACTTATGGTTGCCAGATGAATGAACATGAT
ACAGAAGTAATGGCAGGCATCTTTGAAGCTCTAGAATATGAAGCGACAAGTGATGTCAATGATGCAGATGTTATTTTACT
CAATACGTGTGCTATTCGTGAAAATGCAGAGAATAAAGTTTTCGGTGAGATTGGGAATCTTAAACATATTAAACAGGCGA
AACCAGATGTACTCATCGGTGTATGTGGATGTATGTCACAAGAAGAGTCCGTAGTGAATAAGATATTAAAATCATATCAA
AATGTGGACATGATCTTTGGGACACATAATATACATCGTCTGCCTGCCATACTGGATGAAGCCTATATGTCTAAAGCGAT
GGTTGTAGAAGTGTGGTCTAAAGAGGGCGATGTTATTGAAAACCTACCTAAGTCACGCCTCGGAGATACGAAAGCATGGG
TCAACATTATGTACGGCTGTGATAAATTCTGTACATATTGTATTGTGCCGTTCACGCGCGGTAAAGAACGTAGTCGTATG
CCAGAAGAGATTATTGCAGAAGTGCGTGATCTTGCGCGACGTGGGTACAAGGAAATTTGTTTACTTGGACAAAACGTAAA
TGCTTATGGTAAAGATATCGATGGATTGAACTATGGATTGGGTGATCTACTTGCGGATATTCAGAAGATTGATATTCCAC
GTGTACGTTTCACTACAAGTCATCCATGGGACTTTGATGATCGACTTATTGAAGTGATCGCTGCAGGAGGAAATATTGTA
CCGCATATCCACCTTCCTGTTCAAAGTGGTAACAATGAAGTATTGAAGATCATGGGGCGTAAGTATTCGCGTGAAAGCTA
TTTGGAGCTGGTTGGTAAAATCAAAGCAGCGATGCCAGAAGTCGCACTGACGACAGATATTATCGTAGGTTATCCGAATG
AAACAGAAGAACAGTTCCAAGAAACGTTATCTCTCTATGAGGAAGTTGCTTTTGACCATGCTTATACTTACTTGTATTCT
CCACGTGAAGGTACACCAGCTGCAAAGATGGAAGATAATGTACCGATGCGTGAGAAAAAGGATCGTCTGCAACGATTAAA
TAAACTTGTTGGTGACTATACTGAACGTGCTTTATCCCATTATCTTGATCAGGAAGTAGTCGTACTATGTGAAGGACCTT
CTAAGAAAAATGATGAAATTCTCGCAGGGTACACTGAAAAGAATAAATTAGTGAACTTTAATGGGCCGAAAGAAGCAATT
GGACAGCTTGTTAAAGTAAGAATCACAGAAACAAAACAATATTCAATGAACGGCGAATTAATAGAAATTATTAAACCAGT
TGAGGTGTAG

Upstream 100 bases:

>100_bases
TTTATGAGAGGTATATCTGTACAATAAAAATACTTTGAAAATATTTGATTTAAAAGCTATACTATAGTAATGATATTATG
AATAAAATGGGGTTATTTAA

Downstream 100 bases:

>100_bases
AGATGTATACAAACGATGAAATTTTGGCAAAAGCAAAAGAATTAGGTAAGATGATGGCAGAAACAGAAGCAGTTGAGTTC
TTTAAACGTGCAGAGGCTCA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEEGRLQSSGAKGNAVPEKDYSKYFEHVYQGPNLKEAKRRGKEDVKYHNDFKIDEQYRGLGRGRKFYIRTYGCQMNEHD
TEVMAGIFEALEYEATSDVNDADVILLNTCAIRENAENKVFGEIGNLKHIKQAKPDVLIGVCGCMSQEESVVNKILKSYQ
NVDMIFGTHNIHRLPAILDEAYMSKAMVVEVWSKEGDVIENLPKSRLGDTKAWVNIMYGCDKFCTYCIVPFTRGKERSRM
PEEIIAEVRDLARRGYKEICLLGQNVNAYGKDIDGLNYGLGDLLADIQKIDIPRVRFTTSHPWDFDDRLIEVIAAGGNIV
PHIHLPVQSGNNEVLKIMGRKYSRESYLELVGKIKAAMPEVALTTDIIVGYPNETEEQFQETLSLYEEVAFDHAYTYLYS
PREGTPAAKMEDNVPMREKKDRLQRLNKLVGDYTERALSHYLDQEVVVLCEGPSKKNDEILAGYTEKNKLVNFNGPKEAI
GQLVKVRITETKQYSMNGELIEIIKPVEV

Sequences:

>Translated_509_residues
MNEEGRLQSSGAKGNAVPEKDYSKYFEHVYQGPNLKEAKRRGKEDVKYHNDFKIDEQYRGLGRGRKFYIRTYGCQMNEHD
TEVMAGIFEALEYEATSDVNDADVILLNTCAIRENAENKVFGEIGNLKHIKQAKPDVLIGVCGCMSQEESVVNKILKSYQ
NVDMIFGTHNIHRLPAILDEAYMSKAMVVEVWSKEGDVIENLPKSRLGDTKAWVNIMYGCDKFCTYCIVPFTRGKERSRM
PEEIIAEVRDLARRGYKEICLLGQNVNAYGKDIDGLNYGLGDLLADIQKIDIPRVRFTTSHPWDFDDRLIEVIAAGGNIV
PHIHLPVQSGNNEVLKIMGRKYSRESYLELVGKIKAAMPEVALTTDIIVGYPNETEEQFQETLSLYEEVAFDHAYTYLYS
PREGTPAAKMEDNVPMREKKDRLQRLNKLVGDYTERALSHYLDQEVVVLCEGPSKKNDEILAGYTEKNKLVNFNGPKEAI
GQLVKVRITETKQYSMNGELIEIIKPVEV
>Mature_509_residues
MNEEGRLQSSGAKGNAVPEKDYSKYFEHVYQGPNLKEAKRRGKEDVKYHNDFKIDEQYRGLGRGRKFYIRTYGCQMNEHD
TEVMAGIFEALEYEATSDVNDADVILLNTCAIRENAENKVFGEIGNLKHIKQAKPDVLIGVCGCMSQEESVVNKILKSYQ
NVDMIFGTHNIHRLPAILDEAYMSKAMVVEVWSKEGDVIENLPKSRLGDTKAWVNIMYGCDKFCTYCIVPFTRGKERSRM
PEEIIAEVRDLARRGYKEICLLGQNVNAYGKDIDGLNYGLGDLLADIQKIDIPRVRFTTSHPWDFDDRLIEVIAAGGNIV
PHIHLPVQSGNNEVLKIMGRKYSRESYLELVGKIKAAMPEVALTTDIIVGYPNETEEQFQETLSLYEEVAFDHAYTYLYS
PREGTPAAKMEDNVPMREKKDRLQRLNKLVGDYTERALSHYLDQEVVVLCEGPSKKNDEILAGYTEKNKLVNFNGPKEAI
GQLVKVRITETKQYSMNGELIEIIKPVEV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=36.0995850622407, Blast_Score=282, Evalue=6e-76,
Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=36.0995850622407, Blast_Score=281, Evalue=9e-76,
Organism=Homo sapiens, GI93277076, Length=447, Percent_Identity=29.7539149888143, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=41.8918918918919, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=26.7857142857143, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=33.1210191082803, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=367, Percent_Identity=28.0653950953678, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21356207, Length=530, Percent_Identity=34.5283018867924, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI19922432, Length=461, Percent_Identity=29.7180043383948, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 57881; Mature: 57881

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEEGRLQSSGAKGNAVPEKDYSKYFEHVYQGPNLKEAKRRGKEDVKYHNDFKIDEQYRG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCCCCCCCHHHHC
LGRGRKFYIRTYGCQMNEHDTEVMAGIFEALEYEATSDVNDADVILLNTCAIRENAENKV
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCH
FGEIGNLKHIKQAKPDVLIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHRLPAILDE
HHHHCCHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
AYMSKAMVVEVWSKEGDVIENLPKSRLGDTKAWVNIMYGCDKFCTYCIVPFTRGKERSRM
HHHHHHHEEEECCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHCCC
PEEIIAEVRDLARRGYKEICLLGQNVNAYGKDIDGLNYGLGDLLADIQKIDIPRVRFTTS
CHHHHHHHHHHHHCCHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECC
HPWDFDDRLIEVIAAGGNIVPHIHLPVQSGNNEVLKIMGRKYSRESYLELVGKIKAAMPE
CCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCC
VALTTDIIVGYPNETEEQFQETLSLYEEVAFDHAYTYLYSPREGTPAAKMEDNVPMREKK
CEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHH
DRLQRLNKLVGDYTERALSHYLDQEVVVLCEGPSKKNDEILAGYTEKNKLVNFNGPKEAI
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCEEECCCHHHHH
GQLVKVRITETKQYSMNGELIEIIKPVEV
HHHHEEEEECCCEECCCCCCEECCCCCCC
>Mature Secondary Structure
MNEEGRLQSSGAKGNAVPEKDYSKYFEHVYQGPNLKEAKRRGKEDVKYHNDFKIDEQYRG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCCCCCCCHHHHC
LGRGRKFYIRTYGCQMNEHDTEVMAGIFEALEYEATSDVNDADVILLNTCAIRENAENKV
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCH
FGEIGNLKHIKQAKPDVLIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHRLPAILDE
HHHHCCHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
AYMSKAMVVEVWSKEGDVIENLPKSRLGDTKAWVNIMYGCDKFCTYCIVPFTRGKERSRM
HHHHHHHEEEECCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHCCC
PEEIIAEVRDLARRGYKEICLLGQNVNAYGKDIDGLNYGLGDLLADIQKIDIPRVRFTTS
CHHHHHHHHHHHHCCHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECC
HPWDFDDRLIEVIAAGGNIVPHIHLPVQSGNNEVLKIMGRKYSRESYLELVGKIKAAMPE
CCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCC
VALTTDIIVGYPNETEEQFQETLSLYEEVAFDHAYTYLYSPREGTPAAKMEDNVPMREKK
CEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHH
DRLQRLNKLVGDYTERALSHYLDQEVVVLCEGPSKKNDEILAGYTEKNKLVNFNGPKEAI
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCEEECCCHHHHH
GQLVKVRITETKQYSMNGELIEIIKPVEV
HHHHEEEEECCCEECCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA