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The map label for this gene is pnpA

Identifier: 222151113

GI number: 222151113

Start: 948815

End: 950926

Strand: Direct

Name: pnpA

Synonym: MCCL_0864

Alternate gene names: 222151113

Gene position: 948815-950926 (Clockwise)

Preceding gene: 222151112

Following gene: 222151114

Centisome position: 45.13

GC content: 41.76

Gene sequence:

>2112_bases
ATGTCTCAAGAAAAGAAAGTTTTTAAAACAGAATGGGCAGGTCGTCCATTAATTATTGAAACTGGCCAGTTAGCGAAACA
AGCAAATGGTGCAGTACTCGTACGCTACGGGGATACGGTAGTATTATCAACGGCGACAGCGAGTAAAGAACCTAGAGATG
TGGACTTCTTCCCGTTAATGGTTAATTATGAAGAGAAATTATATGCAGCAGGTAAAATACCTGGAGGATTCAACAAACGT
GAAGGCCGTCCGGGTGAGGATGCTACTTTGACTTCACGATTGATTGACCGCCCGATTCGTCCATTATTCCCAAAAGGTTA
TCGTCATGATGTTCAGGTCATTTCTATCGTTATGAGTGTAGATCCGGATAATTCACCTGAAATGGCAGCGATGATCGGTT
CATCTATGGCGCTTGCGGTTTCAGATATTCCATTTGAAGGACCAATTGCAGGTGTAAATGTAGGTTTAGTTGATGGAGAA
CTCATCATAAATCCTGACGTTCAGCAGCGCGAAGTTTCTGTTCTGGACTTACAAGTTGCTGGTCACTTCGATGCGGTCAA
CATGGTTGAAGCGGGTGCAAAAGAAGTAGCGGAAGATAAGATGCTTGAAGCAATCATGTTCGGTCATGCTGAAATCAAGA
AACTTGTTGAATTCCAGCAATCGATCATTGATGAGATACAACCTGTAAAGTCTGAGTTTGTACCTGTTGAAGTGGATGCA
GACTTAGAATCAAAGGTAGAACAATTATCAGAAAGCTTTGGACTATCTCAAGCGATTCAGACTCAGGAAAAGCTTGCGCG
TGAGGAAAATATTACAGCAATCAAATTAAAGGTTATCGAAGCTTTTGAAGGTGAAGATGAAGCTGTTATCACTGCAGTGA
ACAAGAAGTTTGATGCACTGATCAAAGAAGAAGTGCGTCGTCTTATCACAGAAGAAAAAGTGCGCCCGGATGGACGTCGC
CCAGACGAAATTCGACCGCTTGATTCAGAAGTCGGTATTTTACCACGTGTACATGGTTCGGGATTATTTACACGTGGACA
AACGCAGGCATTATCAGTTGCAACACTTGGTGCGCTAGGTGAGCATCAGATTATCGATGGGCTTGGTGTAGAAGAAGAGA
AGCGTTATATGCATCATTATAACTTCCCGAATTTCTCTGTAGGTGAGACTGGGCCAATTCGCGCACCAGGTCGTCGTGAA
ATTGGCCATGGTGCACTTGGTGAACGTGCGCTATTGCAGGTCATTCCAGATGAAAAAGAATTCCCATATACAATTCGTGT
TGTATCGGAGGTTTTAGAATCAAATGGTTCTAGTTCACAAGCATCAATCTGTGGTTCTACGCTTGCCTTGATGGATGCGG
GTGTACCTATTAAAGCACCAGTTGCGGGTATTGCAATGGGTCTTGTAACAAAAGATGAAAATTATACAATTCTATCTGAT
ATCCAAGGTATGGAAGACGCACTTGGTGATATGGACTTTAAAGTTGCAGGGACAAAAGAAGGTATCACAGCAATACAGAT
GGACATTAAGATTAATGGGTTAACGGAAGATATCTTACGTGAAGCATTAGAGCAGGCGCGAGTAGGTCGTCTGCATATTA
TGGAACATATGTTATCTACAATTTCTGAACCAAGAGCAGAACTTAGCCAATACGCACCTAAAATTGAAATTATTCATATT
AATCCTGATAAGATTCGTGATGTTATCGGACCAGGTGGTAAGAAAATCAACGAAATTATCGATGCAACAGGAGTTAAGCT
TGATATTGAACAAGATGGTACAGTGTTCATCGGTTCAAGTGATGCATCAATGATAGAGGCGGCGAAGAAGTTGATCGAAA
ATATTGTACGTGAAGCTGAGGTTGGCCAGATTTATATGGCAACGGTAAAACGTATCGAGAAGTTCGGTGCATTTGTTGAA
ATCTTCCCAGGTAAAGATGCGCTCGTACATATCTCGCAAATTGCTTTAGAACGTATAAACAAAGTAGAAGATGTTGTGAA
GCTTGGCGATCAGTTCCTGGTGAAAGTCACTGAGATTGATAAACAAGGTCGCGTAAATGCATCACGAAAAGTGCTGTTAG
AAGAAGAGAAGAAAGCATCAGAAGAAAAATAG

Upstream 100 bases:

>100_bases
AAAGAAACCTATGTTTCTTTCGTTTTTTTTATGCTATTTATGACATAATATGAATTGTCATGTTATGATTAAGGATATAT
GTGAGAGAGGAGATACATAC

Downstream 100 bases:

>100_bases
AGCATTAAGGTGAGGCGATATGTCTCACCTTTTTATTTGATTTGTCTGTGAGGAGATTATTTGGAGCATATTAAACTTAA
GAATGATATGACGTTTGCAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MSQEKKVFKTEWAGRPLIIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDVDFFPLMVNYEEKLYAAGKIPGGFNKR
EGRPGEDATLTSRLIDRPIRPLFPKGYRHDVQVISIVMSVDPDNSPEMAAMIGSSMALAVSDIPFEGPIAGVNVGLVDGE
LIINPDVQQREVSVLDLQVAGHFDAVNMVEAGAKEVAEDKMLEAIMFGHAEIKKLVEFQQSIIDEIQPVKSEFVPVEVDA
DLESKVEQLSESFGLSQAIQTQEKLAREENITAIKLKVIEAFEGEDEAVITAVNKKFDALIKEEVRRLITEEKVRPDGRR
PDEIRPLDSEVGILPRVHGSGLFTRGQTQALSVATLGALGEHQIIDGLGVEEEKRYMHHYNFPNFSVGETGPIRAPGRRE
IGHGALGERALLQVIPDEKEFPYTIRVVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTKDENYTILSD
IQGMEDALGDMDFKVAGTKEGITAIQMDIKINGLTEDILREALEQARVGRLHIMEHMLSTISEPRAELSQYAPKIEIIHI
NPDKIRDVIGPGGKKINEIIDATGVKLDIEQDGTVFIGSSDASMIEAAKKLIENIVREAEVGQIYMATVKRIEKFGAFVE
IFPGKDALVHISQIALERINKVEDVVKLGDQFLVKVTEIDKQGRVNASRKVLLEEEKKASEEK

Sequences:

>Translated_703_residues
MSQEKKVFKTEWAGRPLIIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDVDFFPLMVNYEEKLYAAGKIPGGFNKR
EGRPGEDATLTSRLIDRPIRPLFPKGYRHDVQVISIVMSVDPDNSPEMAAMIGSSMALAVSDIPFEGPIAGVNVGLVDGE
LIINPDVQQREVSVLDLQVAGHFDAVNMVEAGAKEVAEDKMLEAIMFGHAEIKKLVEFQQSIIDEIQPVKSEFVPVEVDA
DLESKVEQLSESFGLSQAIQTQEKLAREENITAIKLKVIEAFEGEDEAVITAVNKKFDALIKEEVRRLITEEKVRPDGRR
PDEIRPLDSEVGILPRVHGSGLFTRGQTQALSVATLGALGEHQIIDGLGVEEEKRYMHHYNFPNFSVGETGPIRAPGRRE
IGHGALGERALLQVIPDEKEFPYTIRVVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTKDENYTILSD
IQGMEDALGDMDFKVAGTKEGITAIQMDIKINGLTEDILREALEQARVGRLHIMEHMLSTISEPRAELSQYAPKIEIIHI
NPDKIRDVIGPGGKKINEIIDATGVKLDIEQDGTVFIGSSDASMIEAAKKLIENIVREAEVGQIYMATVKRIEKFGAFVE
IFPGKDALVHISQIALERINKVEDVVKLGDQFLVKVTEIDKQGRVNASRKVLLEEEKKASEEK
>Mature_702_residues
SQEKKVFKTEWAGRPLIIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDVDFFPLMVNYEEKLYAAGKIPGGFNKRE
GRPGEDATLTSRLIDRPIRPLFPKGYRHDVQVISIVMSVDPDNSPEMAAMIGSSMALAVSDIPFEGPIAGVNVGLVDGEL
IINPDVQQREVSVLDLQVAGHFDAVNMVEAGAKEVAEDKMLEAIMFGHAEIKKLVEFQQSIIDEIQPVKSEFVPVEVDAD
LESKVEQLSESFGLSQAIQTQEKLAREENITAIKLKVIEAFEGEDEAVITAVNKKFDALIKEEVRRLITEEKVRPDGRRP
DEIRPLDSEVGILPRVHGSGLFTRGQTQALSVATLGALGEHQIIDGLGVEEEKRYMHHYNFPNFSVGETGPIRAPGRREI
GHGALGERALLQVIPDEKEFPYTIRVVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTKDENYTILSDI
QGMEDALGDMDFKVAGTKEGITAIQMDIKINGLTEDILREALEQARVGRLHIMEHMLSTISEPRAELSQYAPKIEIIHIN
PDKIRDVIGPGGKKINEIIDATGVKLDIEQDGTVFIGSSDASMIEAAKKLIENIVREAEVGQIYMATVKRIEKFGAFVEI
FPGKDALVHISQIALERINKVEDVVKLGDQFLVKVTEIDKQGRVNASRKVLLEEEKKASEEK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=709, Percent_Identity=37.7997179125529, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI9506689, Length=224, Percent_Identity=27.6785714285714, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=48.7734487734488, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=714, Percent_Identity=32.3529411764706, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI281362905, Length=716, Percent_Identity=37.9888268156425, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24651641, Length=716, Percent_Identity=37.9888268156425, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24651643, Length=716, Percent_Identity=37.9888268156425, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI161079377, Length=667, Percent_Identity=37.4812593703148, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_MACCJ (B9EBG0)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560267.1
- ProteinModelPortal:   B9EBG0
- GeneID:   7389508
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0864
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77092; Mature: 76961

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEKKVFKTEWAGRPLIIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDVDFFPLM
CCCCCCHHHHHCCCCCEEEECCHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCEEEEE
VNYEEKLYAAGKIPGGFNKREGRPGEDATLTSRLIDRPIRPLFPKGYRHDVQVISIVMSV
ECCCHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEC
DPDNSPEMAAMIGSSMALAVSDIPFEGPIAGVNVGLVDGELIINPDVQQREVSVLDLQVA
CCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCEEEECCCCCCCCEEEEEEEEC
GHFDAVNMVEAGAKEVAEDKMLEAIMFGHAEIKKLVEFQQSIIDEIQPVKSEFVPVEVDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
DLESKVEQLSESFGLSQAIQTQEKLAREENITAIKLKVIEAFEGEDEAVITAVNKKFDAL
CHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEEEEEHHCCCCCEEEEEHHHHHHHH
IKEEVRRLITEEKVRPDGRRPDEIRPLDSEVGILPRVHGSGLFTRGQTQALSVATLGALG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCEECCCCCEEHHHHHHCCC
EHQIIDGLGVEEEKRYMHHYNFPNFSVGETGPIRAPGRREIGHGALGERALLQVIPDEKE
CCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
FPYTIRVVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTKDENYTILSD
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEECCCCEEEHHH
IQGMEDALGDMDFKVAGTKEGITAIQMDIKINGLTEDILREALEQARVGRLHIMEHMLST
HCHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHH
ISEPRAELSQYAPKIEIIHINPDKIRDVIGPGGKKINEIIDATGVKLDIEQDGTVFIGSS
HCCHHHHHHHCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCEEEEECC
DASMIEAAKKLIENIVREAEVGQIYMATVKRIEKFGAFVEIFPGKDALVHISQIALERIN
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHH
KVEDVVKLGDQFLVKVTEIDKQGRVNASRKVLLEEEKKASEEK
HHHHHHHCCHHHEEEEECCCCCCCCCCHHHHEEHHHHHCCCCC
>Mature Secondary Structure 
SQEKKVFKTEWAGRPLIIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDVDFFPLM
CCCCCHHHHHCCCCCEEEECCHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCEEEEE
VNYEEKLYAAGKIPGGFNKREGRPGEDATLTSRLIDRPIRPLFPKGYRHDVQVISIVMSV
ECCCHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEC
DPDNSPEMAAMIGSSMALAVSDIPFEGPIAGVNVGLVDGELIINPDVQQREVSVLDLQVA
CCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCEEEECCCCCCCCEEEEEEEEC
GHFDAVNMVEAGAKEVAEDKMLEAIMFGHAEIKKLVEFQQSIIDEIQPVKSEFVPVEVDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
DLESKVEQLSESFGLSQAIQTQEKLAREENITAIKLKVIEAFEGEDEAVITAVNKKFDAL
CHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEEEEEHHCCCCCEEEEEHHHHHHHH
IKEEVRRLITEEKVRPDGRRPDEIRPLDSEVGILPRVHGSGLFTRGQTQALSVATLGALG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCEECCCCCEEHHHHHHCCC
EHQIIDGLGVEEEKRYMHHYNFPNFSVGETGPIRAPGRREIGHGALGERALLQVIPDEKE
CCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
FPYTIRVVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTKDENYTILSD
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEECCCCEEEHHH
IQGMEDALGDMDFKVAGTKEGITAIQMDIKINGLTEDILREALEQARVGRLHIMEHMLST
HCHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHH
ISEPRAELSQYAPKIEIIHINPDKIRDVIGPGGKKINEIIDATGVKLDIEQDGTVFIGSS
HCCHHHHHHHCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCEEEEECC
DASMIEAAKKLIENIVREAEVGQIYMATVKRIEKFGAFVEIFPGKDALVHISQIALERIN
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHH
KVEDVVKLGDQFLVKVTEIDKQGRVNASRKVLLEEEKKASEEK
HHHHHHHCCHHHEEEEECCCCCCCCCCHHHHEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA