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The map label for this gene is infB

Identifier: 222151102

GI number: 222151102

Start: 932790

End: 934970

Strand: Direct

Name: infB

Synonym: MCCL_0853

Alternate gene names: 222151102

Gene position: 932790-934970 (Clockwise)

Preceding gene: 222151101

Following gene: 222151103

Centisome position: 44.37

GC content: 39.71

Gene sequence:

>2181_bases
ATGAGTAAAAAGAGAATATATGAATACGCAAAAGAAGTCAGCTTAAAAAGTAAAGATATTATCGATGCATTAAAGAAAAT
GAACATCGAAGCATCGAGTCACATGCAGGTAATCGAAGCAAAAGAAATTGCTGCATTAGATAAGATCTTCAAGAAATCAG
ATACCAAATCAGAAGTGAAATCTGAGCAAAAAGCTGAAGCAAAAAAAGAAGAACCGAACAAAGAACAGCCTAAAAAAGAA
GAACCGAAAAAGCAAAGTGATCAGAAGAGACCACAAAATAATAATCAAAAACGTAATCCAAACCATCGTCCTGGAAGCCA
TAATAAACCAGGTACGGGCGGTCCATCTAAAAACAAAAAAGGTAAAGGCAAAGGTAAACAAAAACCTGAACCAAAACAAG
AAGTACCTGTTGTAAAAGAAACACCTTCTAAAATTACGTATGAAGAAGGCATTACAGTAGGAGAGCTGGCTGAAAAACTA
GGTAAAGATGCTTCTGAAATCGTTAAAAATTTATTTATGGTCGGTATTATGGCTAATATCAATCAATCTTTAAACCAAGA
AGCGATCGAGCTTATTTGTGATGAGTATGGCGTAGAAGCTGAACTAGAAGTTGTGGTCGATGCAACGGATCTAGAAACAT
ATTTTGAAGATGTAGATGCTAATGAAGAGGATATTATGGAACGTCCGCCTGTTGTTACAATTATGGGACACGTTGACCAT
GGTAAAACTACATTACTTGATTCTATCCGTAATACACGTGTTACTGCAGGAGAAGCAGGAGGAATCACACAGCATATCGG
TGCATATCAAATTGAATATAATGATAAGCCTATTACATTCCTTGATACACCAGGGCATGCTGCGTTTACGACAATGCGTG
CACGTGGGGCACAAGTAACAGATATCACGATATTAGTAGTAGCAGCTGATGATGGCGTAATGCCACAAACGGTTGAAGCT
ATCAACCATGCGAAAGCAGCAGAAGTACCAATCATCGTCGCTGTAAACAAAATTGATAAGCCGACTGCTAATCCAGACCG
TGTAATGCAGGAACTTGGTGAGCATGGTTTATATCCTGAAGATTGGGGCGGAGACACAATATTTGTGCAGCTTTCTGCGA
TAAAAGGTGATGGTATTGATGACTTGCTTGAAATGATTCAGCTTGTAACTGAAGTGGAAGAATTAAAGGCAAATCCAAAA
CGTACAGCGATTGGAACTGTAATTGAAGCGGAACTAGATAAATCTCGTGGACCAGCTGCATCTTTACTTGTTCAAGATGG
TACCCTTGAAATCGGTGACTCTATCGTTGTTGGTAATACGTTCGGACGTGTTCGTGCAATGGTGAACGACCTTGGCAAAC
GTATTAAGACAGCCGGACCATCTTTACCAGTTGAAATTACTGGACTTCAAGATGTGCCTTTAGCGGGTGATCGTTTTGTT
GTATTTAAAGATGAGAAGAAAGCACGTCGTATTGGTGAAGCACGTCAGCAGCAAAATATTTTAGCGCAGCGTCAGGAATC
TCAAAAAGTTTCGCTTGATAACTTATTTGAACAGATGAAACAAGGTGAGATGAAAGACTTAAACGTTATCATTAAAGGTG
ATGTTCAAGGTTCAGTTGAAGCATTGGCAGCATCATTGATGAAGATCGATGTTGAAGGCGTTAACGTACGTATTATCCAT
ACAGCAGTGGGGGCAATCAATGAATCTGACGTTACTTTAGCGTCTGCATCTAACGGTATTATCATTGGTTTTAACGTCCG
CCCTGATGTTAATGCAAAACGTGCGGCAGAAGCTGAAGGTGTAGATATGCGTTTACACCGTATCATCTATAAAGTGATTG
AAGAAATTGAAAGTGCGATGAAAGGGATGTTAGACCCTGAGTTTGAAGAAAAAGTAATCGGTCAGGCAGAAGTTCGTCAA
ACAATCAATATTTCTAAAGTCGGTACAGTAGCTGGAAGTTATGTGACTGACGGTAAGATTACGCGTGATTCACAAGTACG
TATCATTCGTGACGGTATCGTCGTTTATGAAGGTCAAGTTGATGCATTAAAACGCTTTAAAGACGATGTACGTGAAGTGG
CACAAGGGTATGAGTGCGGTATCACGATCGAAAACTTCAATGACATCAAAGAAGGCGACATCTTTGAGGCCTTTGTCATG
GAAGAGATTAAACGCGCCTAA

Upstream 100 bases:

>100_bases
GCACTTGGTAAAGATGAACGTGTAACAATTGGCGTTCTGGATGCAGGATTTGCCAAGTCAATGAAAGCTTTGATTCGAGA
CATCAATGAGGAGAGAAGCT

Downstream 100 bases:

>100_bases
TAATGCATGGTTAAGCTCTTTCTGAGAGTTGAAAGAAGAACAGTCTAAACTTTTCAAATTACACTTTCAACGAAATATCA
GGAAAATTTACAATGCGAGA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MSKKRIYEYAKEVSLKSKDIIDALKKMNIEASSHMQVIEAKEIAALDKIFKKSDTKSEVKSEQKAEAKKEEPNKEQPKKE
EPKKQSDQKRPQNNNQKRNPNHRPGSHNKPGTGGPSKNKKGKGKGKQKPEPKQEVPVVKETPSKITYEEGITVGELAEKL
GKDASEIVKNLFMVGIMANINQSLNQEAIELICDEYGVEAELEVVVDATDLETYFEDVDANEEDIMERPPVVTIMGHVDH
GKTTLLDSIRNTRVTAGEAGGITQHIGAYQIEYNDKPITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEA
INHAKAAEVPIIVAVNKIDKPTANPDRVMQELGEHGLYPEDWGGDTIFVQLSAIKGDGIDDLLEMIQLVTEVEELKANPK
RTAIGTVIEAELDKSRGPAASLLVQDGTLEIGDSIVVGNTFGRVRAMVNDLGKRIKTAGPSLPVEITGLQDVPLAGDRFV
VFKDEKKARRIGEARQQQNILAQRQESQKVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIH
TAVGAINESDVTLASASNGIIIGFNVRPDVNAKRAAEAEGVDMRLHRIIYKVIEEIESAMKGMLDPEFEEKVIGQAEVRQ
TINISKVGTVAGSYVTDGKITRDSQVRIIRDGIVVYEGQVDALKRFKDDVREVAQGYECGITIENFNDIKEGDIFEAFVM
EEIKRA

Sequences:

>Translated_726_residues
MSKKRIYEYAKEVSLKSKDIIDALKKMNIEASSHMQVIEAKEIAALDKIFKKSDTKSEVKSEQKAEAKKEEPNKEQPKKE
EPKKQSDQKRPQNNNQKRNPNHRPGSHNKPGTGGPSKNKKGKGKGKQKPEPKQEVPVVKETPSKITYEEGITVGELAEKL
GKDASEIVKNLFMVGIMANINQSLNQEAIELICDEYGVEAELEVVVDATDLETYFEDVDANEEDIMERPPVVTIMGHVDH
GKTTLLDSIRNTRVTAGEAGGITQHIGAYQIEYNDKPITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEA
INHAKAAEVPIIVAVNKIDKPTANPDRVMQELGEHGLYPEDWGGDTIFVQLSAIKGDGIDDLLEMIQLVTEVEELKANPK
RTAIGTVIEAELDKSRGPAASLLVQDGTLEIGDSIVVGNTFGRVRAMVNDLGKRIKTAGPSLPVEITGLQDVPLAGDRFV
VFKDEKKARRIGEARQQQNILAQRQESQKVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIH
TAVGAINESDVTLASASNGIIIGFNVRPDVNAKRAAEAEGVDMRLHRIIYKVIEEIESAMKGMLDPEFEEKVIGQAEVRQ
TINISKVGTVAGSYVTDGKITRDSQVRIIRDGIVVYEGQVDALKRFKDDVREVAQGYECGITIENFNDIKEGDIFEAFVM
EEIKRA
>Mature_725_residues
SKKRIYEYAKEVSLKSKDIIDALKKMNIEASSHMQVIEAKEIAALDKIFKKSDTKSEVKSEQKAEAKKEEPNKEQPKKEE
PKKQSDQKRPQNNNQKRNPNHRPGSHNKPGTGGPSKNKKGKGKGKQKPEPKQEVPVVKETPSKITYEEGITVGELAEKLG
KDASEIVKNLFMVGIMANINQSLNQEAIELICDEYGVEAELEVVVDATDLETYFEDVDANEEDIMERPPVVTIMGHVDHG
KTTLLDSIRNTRVTAGEAGGITQHIGAYQIEYNDKPITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEAI
NHAKAAEVPIIVAVNKIDKPTANPDRVMQELGEHGLYPEDWGGDTIFVQLSAIKGDGIDDLLEMIQLVTEVEELKANPKR
TAIGTVIEAELDKSRGPAASLLVQDGTLEIGDSIVVGNTFGRVRAMVNDLGKRIKTAGPSLPVEITGLQDVPLAGDRFVV
FKDEKKARRIGEARQQQNILAQRQESQKVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIHT
AVGAINESDVTLASASNGIIIGFNVRPDVNAKRAAEAEGVDMRLHRIIYKVIEEIESAMKGMLDPEFEEKVIGQAEVRQT
INISKVGTVAGSYVTDGKITRDSQVRIIRDGIVVYEGQVDALKRFKDDVREVAQGYECGITIENFNDIKEGDIFEAFVME
EIKRA

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=522, Percent_Identity=41.1877394636015, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI53729337, Length=522, Percent_Identity=41.1877394636015, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI84043963, Length=474, Percent_Identity=27.4261603375527, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI157426893, Length=240, Percent_Identity=28.3333333333333, Blast_Score=83, Evalue=9e-16,
Organism=Escherichia coli, GI1789559, Length=577, Percent_Identity=53.2062391681109, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1788922, Length=232, Percent_Identity=28.8793103448276, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI48994988, Length=144, Percent_Identity=35.4166666666667, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI2367247, Length=229, Percent_Identity=31.4410480349345, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790412, Length=127, Percent_Identity=39.3700787401575, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789737, Length=127, Percent_Identity=39.3700787401575, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=544, Percent_Identity=35.6617647058824, Blast_Score=309, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI212656558, Length=567, Percent_Identity=26.63139329806, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=29.0322580645161, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556456, Length=244, Percent_Identity=31.1475409836066, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324550, Length=631, Percent_Identity=36.1331220285262, Blast_Score=357, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6319282, Length=464, Percent_Identity=27.1551724137931, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=122, Percent_Identity=37.7049180327869, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28572034, Length=649, Percent_Identity=36.8258859784284, Blast_Score=352, Evalue=6e-97,
Organism=Drosophila melanogaster, GI24656849, Length=466, Percent_Identity=26.8240343347639, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI160714833, Length=78, Percent_Identity=69.2307692307692, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI78706572, Length=226, Percent_Identity=28.7610619469027, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281363316, Length=152, Percent_Identity=34.8684210526316, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17864358, Length=152, Percent_Identity=34.8684210526316, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_MACCJ (B9EBE9)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560256.1
- GeneID:   7389960
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0853
- OMA:   EAYVMKE
- ProtClustDB:   PRK05306
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 79858; Mature: 79727

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKRIYEYAKEVSLKSKDIIDALKKMNIEASSHMQVIEAKEIAALDKIFKKSDTKSEVK
CCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
SEQKAEAKKEEPNKEQPKKEEPKKQSDQKRPQNNNQKRNPNHRPGSHNKPGTGGPSKNKK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GKGKGKQKPEPKQEVPVVKETPSKITYEEGITVGELAEKLGKDASEIVKNLFMVGIMANI
CCCCCCCCCCCHHHCCCCCCCCCCEEHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NQSLNQEAIELICDEYGVEAELEVVVDATDLETYFEDVDANEEDIMERPPVVTIMGHVDH
HHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCC
GKTTLLDSIRNTRVTAGEAGGITQHIGAYQIEYNDKPITFLDTPGHAAFTTMRARGAQVT
CHHHHHHHHHCCEECCCCCCCCHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHCCCEEE
DITILVVAADDGVMPQTVEAINHAKAAEVPIIVAVNKIDKPTANPDRVMQELGEHGLYPE
EEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCC
DWGGDTIFVQLSAIKGDGIDDLLEMIQLVTEVEELKANPKRTAIGTVIEAELDKSRGPAA
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCE
SLLVQDGTLEIGDSIVVGNTFGRVRAMVNDLGKRIKTAGPSLPVEITGLQDVPLAGDRFV
EEEEECCCEEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEE
VFKDEKKARRIGEARQQQNILAQRQESQKVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVE
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHH
ALAASLMKIDVEGVNVRIIHTAVGAINESDVTLASASNGIIIGFNVRPDVNAKRAAEAEG
HHHHHHHHEEECCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHCCCCC
VDMRLHRIIYKVIEEIESAMKGMLDPEFEEKVIGQAEVRQTINISKVGTVAGSYVTDGKI
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHCCHHHHHHCCCCEEECCEE
TRDSQVRIIRDGIVVYEGQVDALKRFKDDVREVAQGYECGITIENFNDIKEGDIFEAFVM
CCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHH
EEIKRA
HHHHCC
>Mature Secondary Structure 
SKKRIYEYAKEVSLKSKDIIDALKKMNIEASSHMQVIEAKEIAALDKIFKKSDTKSEVK
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
SEQKAEAKKEEPNKEQPKKEEPKKQSDQKRPQNNNQKRNPNHRPGSHNKPGTGGPSKNKK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GKGKGKQKPEPKQEVPVVKETPSKITYEEGITVGELAEKLGKDASEIVKNLFMVGIMANI
CCCCCCCCCCCHHHCCCCCCCCCCEEHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NQSLNQEAIELICDEYGVEAELEVVVDATDLETYFEDVDANEEDIMERPPVVTIMGHVDH
HHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCC
GKTTLLDSIRNTRVTAGEAGGITQHIGAYQIEYNDKPITFLDTPGHAAFTTMRARGAQVT
CHHHHHHHHHCCEECCCCCCCCHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHCCCEEE
DITILVVAADDGVMPQTVEAINHAKAAEVPIIVAVNKIDKPTANPDRVMQELGEHGLYPE
EEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCC
DWGGDTIFVQLSAIKGDGIDDLLEMIQLVTEVEELKANPKRTAIGTVIEAELDKSRGPAA
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCE
SLLVQDGTLEIGDSIVVGNTFGRVRAMVNDLGKRIKTAGPSLPVEITGLQDVPLAGDRFV
EEEEECCCEEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEE
VFKDEKKARRIGEARQQQNILAQRQESQKVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVE
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHH
ALAASLMKIDVEGVNVRIIHTAVGAINESDVTLASASNGIIIGFNVRPDVNAKRAAEAEG
HHHHHHHHEEECCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHCCCCC
VDMRLHRIIYKVIEEIESAMKGMLDPEFEEKVIGQAEVRQTINISKVGTVAGSYVTDGKI
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHCCHHHHHHCCCCEEECCEE
TRDSQVRIIRDGIVVYEGQVDALKRFKDDVREVAQGYECGITIENFNDIKEGDIFEAFVM
CCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHH
EEIKRA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA