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The map label for this gene is glgB [H]

Identifier: 222151031

GI number: 222151031

Start: 843462

End: 845384

Strand: Direct

Name: glgB [H]

Synonym: MCCL_0782

Alternate gene names: 222151031

Gene position: 843462-845384 (Clockwise)

Preceding gene: 222151027

Following gene: 222151032

Centisome position: 40.12

GC content: 39.94

Gene sequence:

>1923_bases
ATGGTAGCTGAATTAGATCGATTTTTATTTCATCAAGGGACGCATTATGCATCCCAGCAGTTTATGGGTGCTCATTTCAA
TAAGGAGAAAGGTGAGACAACATTTACTGTATGGGCTCCAAATGCGCGTAAGGTAAGTATTGCGTTAGACGCGAATCAAT
GGACGGGTGATGGATACAAACTTGAGAAAGTACCGGATAGTGGATTGTTTACAGGTACATTTAATGTAGATGTCGGTTCA
GCCTATAAGTATTTAATCGAAACTAAGCAGGGTGAACAACTATTCAAGGCGGATCCTTACGCATTCTATGCTGAGAAACG
GCCTGCAACAGCATCGATTGTCACGGATATCTCCTATCAATGGTCGGATGAGGCTTGGCAGAAGAAGAAGACTGCTCCTT
ATAATCAACCAATCAATATTTATGAGGTTCATCTGGGAACGTGGAAAAAACATAATGTTGAGCGCTACGAAGGAGAATCC
GTCGATGCGTTTACAGATCGTTCGTACTATACATATAGAGAAATGGCGGAGTCTTTAATCCCGTATGTGAAGAGGATGGG
CTATACACATATTGAATTGATGCCAATTACCGAACATCCTTTCGATTTATCCTGGGGTTATCAAGTAACCGGTTACTTTG
CACCTACAAGCCGATTTGGTACACCGCAGGATTTCAAATATTTTATCGATCAATGTCATCAGCACCAGATTGGAGTGATA
CTGGACTGGGTACCGGCTCATTTTTGTAAGGATGCGCATGGTCTTAGAATGTTTGATGGGACTCCGTTATTTGAACATCC
GGATATGTATCGTGCAGAGAAGCGTGGATGGGGGACACTGACATTTGATTTTGGTCGAACAGAAGTCCAGAGTTTCCTCG
TGTCGAATGCACTGTTTTGGCTCGGTGAATATCATATCGATGGTTTACGTGTGGATGCAGTGCATTCGATGACTGACCTT
AACTTTGAGAATCATAAAGTGCATGAGAGAATTTTTAATGATGACGGTACAACCATTCATAAAGAAGCGGTTGCGTTTTT
ACAGAAGCTGAACACTGAAGTGTTTAGGCACTATCCACATACGTTAATGATTGCAGAGGATTCATCAGATATGAAGAATG
TCACAGCACCGGTTGCTTCTGGCGGCCTTGGGTTTAACTTTAAATGGGATCTTGGATTTATGCATGACACGCTTGATTAT
ATGGAGCTCGACCCTATTATGAGACAACATTACCACAAATATTTAAACTTTCCGCTTGTCTATCGCTATGACGAAAACTT
TATCCTGCCGTTCTCACATGATGAAGTTGTTCACGGAAAACTTAGTATGCTGGATAAAATGCCGGGGGATCAATGGCGGA
AGTTCGCACAGTATCGCTTACTGTATGGCTATCAAATGACTCAGCCAGGTAAGAAGCTTAACTTTATGGGTAGCGAGATT
GGCATGTATGCTGAATGGAAAGATAAGGAGCAGGTAGATTGGTTTTTGCTTAACTATCCGTATCATAAAGGACTTCAGGA
TTTTGTTCGTGATATGAACAAGTTATATTTGAAAGAAAAGCCATTATTTGAACTTGACTATGAAACACAAGGTTTCAGAT
GGATTGACGCAGATAACAACCAGCAGAACATCGCAAGTTATATGCGTACAGATCGTAAGGGGAATACTTTAGTCATCGTG
CTTAATTTCTCGCCGAATGTCTATTATGACTTTACAGTTGCAGTTGATCATCCTGGTAAATATAAAGAATATTTCAATTC
AGATAAAAAGGAATATGGTGGATCTAATCAGACGATTAGTAAGTATCTTTTTTCTGAATTATTAGAAGTTAACGGTTTCA
AACATGCAATAAAAGGGAAAATACCACCGCTCGGATTTGTAATATATAAAAAAGTTAAAGAAAGAAAGAAACATCATAGT
TAG

Upstream 100 bases:

>100_bases
GGTATAATATAGAGAAACATATAATTAAATAATGTTAAATTAATATAATGTTCACATTTTCCAATTATACTGAAAATAAG
CAATATGACGGAGGGATGAA

Downstream 100 bases:

>100_bases
TACAAAAATATGGATGAGGTAGGGGATATCATGTCAAATGAAGTTGTTGGAATGTTACTTGCAGGTGGAAAGGGAACGCG
ATTAGGTCAGCTGACAAAAA

Product: 1,4-alpha-glucan branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MVAELDRFLFHQGTHYASQQFMGAHFNKEKGETTFTVWAPNARKVSIALDANQWTGDGYKLEKVPDSGLFTGTFNVDVGS
AYKYLIETKQGEQLFKADPYAFYAEKRPATASIVTDISYQWSDEAWQKKKTAPYNQPINIYEVHLGTWKKHNVERYEGES
VDAFTDRSYYTYREMAESLIPYVKRMGYTHIELMPITEHPFDLSWGYQVTGYFAPTSRFGTPQDFKYFIDQCHQHQIGVI
LDWVPAHFCKDAHGLRMFDGTPLFEHPDMYRAEKRGWGTLTFDFGRTEVQSFLVSNALFWLGEYHIDGLRVDAVHSMTDL
NFENHKVHERIFNDDGTTIHKEAVAFLQKLNTEVFRHYPHTLMIAEDSSDMKNVTAPVASGGLGFNFKWDLGFMHDTLDY
MELDPIMRQHYHKYLNFPLVYRYDENFILPFSHDEVVHGKLSMLDKMPGDQWRKFAQYRLLYGYQMTQPGKKLNFMGSEI
GMYAEWKDKEQVDWFLLNYPYHKGLQDFVRDMNKLYLKEKPLFELDYETQGFRWIDADNNQQNIASYMRTDRKGNTLVIV
LNFSPNVYYDFTVAVDHPGKYKEYFNSDKKEYGGSNQTISKYLFSELLEVNGFKHAIKGKIPPLGFVIYKKVKERKKHHS

Sequences:

>Translated_640_residues
MVAELDRFLFHQGTHYASQQFMGAHFNKEKGETTFTVWAPNARKVSIALDANQWTGDGYKLEKVPDSGLFTGTFNVDVGS
AYKYLIETKQGEQLFKADPYAFYAEKRPATASIVTDISYQWSDEAWQKKKTAPYNQPINIYEVHLGTWKKHNVERYEGES
VDAFTDRSYYTYREMAESLIPYVKRMGYTHIELMPITEHPFDLSWGYQVTGYFAPTSRFGTPQDFKYFIDQCHQHQIGVI
LDWVPAHFCKDAHGLRMFDGTPLFEHPDMYRAEKRGWGTLTFDFGRTEVQSFLVSNALFWLGEYHIDGLRVDAVHSMTDL
NFENHKVHERIFNDDGTTIHKEAVAFLQKLNTEVFRHYPHTLMIAEDSSDMKNVTAPVASGGLGFNFKWDLGFMHDTLDY
MELDPIMRQHYHKYLNFPLVYRYDENFILPFSHDEVVHGKLSMLDKMPGDQWRKFAQYRLLYGYQMTQPGKKLNFMGSEI
GMYAEWKDKEQVDWFLLNYPYHKGLQDFVRDMNKLYLKEKPLFELDYETQGFRWIDADNNQQNIASYMRTDRKGNTLVIV
LNFSPNVYYDFTVAVDHPGKYKEYFNSDKKEYGGSNQTISKYLFSELLEVNGFKHAIKGKIPPLGFVIYKKVKERKKHHS
>Mature_640_residues
MVAELDRFLFHQGTHYASQQFMGAHFNKEKGETTFTVWAPNARKVSIALDANQWTGDGYKLEKVPDSGLFTGTFNVDVGS
AYKYLIETKQGEQLFKADPYAFYAEKRPATASIVTDISYQWSDEAWQKKKTAPYNQPINIYEVHLGTWKKHNVERYEGES
VDAFTDRSYYTYREMAESLIPYVKRMGYTHIELMPITEHPFDLSWGYQVTGYFAPTSRFGTPQDFKYFIDQCHQHQIGVI
LDWVPAHFCKDAHGLRMFDGTPLFEHPDMYRAEKRGWGTLTFDFGRTEVQSFLVSNALFWLGEYHIDGLRVDAVHSMTDL
NFENHKVHERIFNDDGTTIHKEAVAFLQKLNTEVFRHYPHTLMIAEDSSDMKNVTAPVASGGLGFNFKWDLGFMHDTLDY
MELDPIMRQHYHKYLNFPLVYRYDENFILPFSHDEVVHGKLSMLDKMPGDQWRKFAQYRLLYGYQMTQPGKKLNFMGSEI
GMYAEWKDKEQVDWFLLNYPYHKGLQDFVRDMNKLYLKEKPLFELDYETQGFRWIDADNNQQNIASYMRTDRKGNTLVIV
LNFSPNVYYDFTVAVDHPGKYKEYFNSDKKEYGGSNQTISKYLFSELLEVNGFKHAIKGKIPPLGFVIYKKVKERKKHHS

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=656, Percent_Identity=27.8963414634146, Blast_Score=192, Evalue=1e-48,
Organism=Escherichia coli, GI1789839, Length=636, Percent_Identity=42.6100628930818, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17554896, Length=617, Percent_Identity=26.742301458671, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI32564391, Length=560, Percent_Identity=26.9642857142857, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320826, Length=662, Percent_Identity=27.9456193353474, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI28573410, Length=665, Percent_Identity=27.3684210526316, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 75058; Mature: 75058

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAELDRFLFHQGTHYASQQFMGAHFNKEKGETTFTVWAPNARKVSIALDANQWTGDGYK
CCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCEE
LEKVPDSGLFTGTFNVDVGSAYKYLIETKQGEQLFKADPYAFYAEKRPATASIVTDISYQ
EEECCCCCEEEEEEEECCCCCEEEEEECCCCCHHHCCCCEEEEECCCCCCEEEEEECCCC
WSDEAWQKKKTAPYNQPINIYEVHLGTWKKHNVERYEGESVDAFTDRSYYTYREMAESLI
CCCHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHH
PYVKRMGYTHIELMPITEHPFDLSWGYQVTGYFAPTSRFGTPQDFKYFIDQCHQHQIGVI
HHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEE
LDWVPAHFCKDAHGLRMFDGTPLFEHPDMYRAEKRGWGTLTFDFGRTEVQSFLVSNALFW
EECHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
LGEYHIDGLRVDAVHSMTDLNFENHKVHERIFNDDGTTIHKEAVAFLQKLNTEVFRHYPH
HHHEEECCEEEEEHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TLMIAEDSSDMKNVTAPVASGGLGFNFKWDLGFMHDTLDYMELDPIMRQHYHKYLNFPLV
EEEEECCCCCHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCHHHHHHHHHHCCCCEE
YRYDENFILPFSHDEVVHGKLSMLDKMPGDQWRKFAQYRLLYGYQMTQPGKKLNFMGSEI
EEECCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCEEECCCCC
GMYAEWKDKEQVDWFLLNYPYHKGLQDFVRDMNKLYLKEKPLFELDYETQGFRWIDADNN
CEEECCCCCCCCCEEEEECCHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCC
QQNIASYMRTDRKGNTLVIVLNFSPNVYYDFTVAVDHPGKYKEYFNSDKKEYGGSNQTIS
HHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHCCCHHHHCCCCHHHH
KYLFSELLEVNGFKHAIKGKIPPLGFVIYKKVKERKKHHS
HHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAELDRFLFHQGTHYASQQFMGAHFNKEKGETTFTVWAPNARKVSIALDANQWTGDGYK
CCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCEE
LEKVPDSGLFTGTFNVDVGSAYKYLIETKQGEQLFKADPYAFYAEKRPATASIVTDISYQ
EEECCCCCEEEEEEEECCCCCEEEEEECCCCCHHHCCCCEEEEECCCCCCEEEEEECCCC
WSDEAWQKKKTAPYNQPINIYEVHLGTWKKHNVERYEGESVDAFTDRSYYTYREMAESLI
CCCHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHH
PYVKRMGYTHIELMPITEHPFDLSWGYQVTGYFAPTSRFGTPQDFKYFIDQCHQHQIGVI
HHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEE
LDWVPAHFCKDAHGLRMFDGTPLFEHPDMYRAEKRGWGTLTFDFGRTEVQSFLVSNALFW
EECHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
LGEYHIDGLRVDAVHSMTDLNFENHKVHERIFNDDGTTIHKEAVAFLQKLNTEVFRHYPH
HHHEEECCEEEEEHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TLMIAEDSSDMKNVTAPVASGGLGFNFKWDLGFMHDTLDYMELDPIMRQHYHKYLNFPLV
EEEEECCCCCHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCHHHHHHHHHHCCCCEE
YRYDENFILPFSHDEVVHGKLSMLDKMPGDQWRKFAQYRLLYGYQMTQPGKKLNFMGSEI
EEECCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCEEECCCCC
GMYAEWKDKEQVDWFLLNYPYHKGLQDFVRDMNKLYLKEKPLFELDYETQGFRWIDADNN
CEEECCCCCCCCCEEEEECCHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCC
QQNIASYMRTDRKGNTLVIVLNFSPNVYYDFTVAVDHPGKYKEYFNSDKKEYGGSNQTIS
HHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHCCCHHHHCCCCHHHH
KYLFSELLEVNGFKHAIKGKIPPLGFVIYKKVKERKKHHS
HHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]