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The map label for this gene is pyrF [H]

Identifier: 222151025

GI number: 222151025

Start: 837365

End: 838063

Strand: Direct

Name: pyrF [H]

Synonym: MCCL_0776

Alternate gene names: 222151025

Gene position: 837365-838063 (Clockwise)

Preceding gene: 222151024

Following gene: 222151026

Centisome position: 39.83

GC content: 40.92

Gene sequence:

>699_bases
ATGAGTAAACCGATTATCGCATTAGATTTTAAAGATATGGATGCTGTCAGAAACTTTTTAAGTCCTTTTGAAGAATCGTT
ATTTGTTAAAGTAGGGATGGAACTTTATTTACAGAATGGCCCGGAGATCATTAAAGAAATTCGTAATCAAGGTCATGAGA
TCTTTCTGGACTTAAAGTTACATGATATTCCGAATACAGTTGGTCAGGCGATGAAAGGTTTAGGAAAACTTGATATTCAG
ATGGTGAATGTACATGCAGCAGGAGGTTCCATCATGATGCAGCGTGCACTGGAAGGGTTACGTGATAGTGGGAGCACCGC
GTCACTCATTGCAGTAACACAGCTGACAAGTACTTCAGAACTCATGATGCAGCAGGAGCAGAACATTCAGTCTTCCATTA
ATGACAGTATTTTAAGCTATGCAACGTTAGCACAGAAGTCTGGTCTTGACGGTGTCGTGTGTTCAGCGAACGAAAGTGCA
ATGATCAGAGACGCATTAGGGGCATCATTCCTGAAAGTGACACCTGGTATTCGTACACTTACAGATGATAAAGGTGATCA
GGTACGTGTGGCGACGCCTGAATCTGCGGCAGAAAATGGTTCAACGCATATCGTTGTAGGACGTTCAATTACTAATGACG
CAAATCCAGTGGAAAAATATAGACAAATTAAGAAAGCGTGGGAATCAAATGTTAAATAA

Upstream 100 bases:

>100_bases
CAGTTTGTAAAGATATCATTGATGCATTACCAGGCTTTCTGGATAAATTAGGCGTTCATCATATTCATGAATTAAAAGGT
CGCACACAGGAGGCAGTTAA

Downstream 100 bases:

>100_bases
AGAAATCGCAAAAGCATTACTTGAAATTGAAGCAGTATCACTACAGCCGAATGATATGTTTACATGGTCTTCTGGTATTA
AAAGCCCGATTTATTGTGAT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSKPIIALDFKDMDAVRNFLSPFEESLFVKVGMELYLQNGPEIIKEIRNQGHEIFLDLKLHDIPNTVGQAMKGLGKLDIQ
MVNVHAAGGSIMMQRALEGLRDSGSTASLIAVTQLTSTSELMMQQEQNIQSSINDSILSYATLAQKSGLDGVVCSANESA
MIRDALGASFLKVTPGIRTLTDDKGDQVRVATPESAAENGSTHIVVGRSITNDANPVEKYRQIKKAWESNVK

Sequences:

>Translated_232_residues
MSKPIIALDFKDMDAVRNFLSPFEESLFVKVGMELYLQNGPEIIKEIRNQGHEIFLDLKLHDIPNTVGQAMKGLGKLDIQ
MVNVHAAGGSIMMQRALEGLRDSGSTASLIAVTQLTSTSELMMQQEQNIQSSINDSILSYATLAQKSGLDGVVCSANESA
MIRDALGASFLKVTPGIRTLTDDKGDQVRVATPESAAENGSTHIVVGRSITNDANPVEKYRQIKKAWESNVK
>Mature_231_residues
SKPIIALDFKDMDAVRNFLSPFEESLFVKVGMELYLQNGPEIIKEIRNQGHEIFLDLKLHDIPNTVGQAMKGLGKLDIQM
VNVHAAGGSIMMQRALEGLRDSGSTASLIAVTQLTSTSELMMQQEQNIQSSINDSILSYATLAQKSGLDGVVCSANESAM
IRDALGASFLKVTPGIRTLTDDKGDQVRVATPESAAENGSTHIVVGRSITNDANPVEKYRQIKKAWESNVK

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=233, Percent_Identity=39.4849785407725, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25236; Mature: 25105

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIIALDFKDMDAVRNFLSPFEESLFVKVGMELYLQNGPEIIKEIRNQGHEIFLDLKL
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEE
HDIPNTVGQAMKGLGKLDIQMVNVHAAGGSIMMQRALEGLRDSGSTASLIAVTQLTSTSE
CCCCHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCHHH
LMMQQEQNIQSSINDSILSYATLAQKSGLDGVVCSANESAMIRDALGASFLKVTPGIRTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHCCHHEEECCCCEEC
TDDKGDQVRVATPESAAENGSTHIVVGRSITNDANPVEKYRQIKKAWESNVK
CCCCCCEEEEECCCCHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKPIIALDFKDMDAVRNFLSPFEESLFVKVGMELYLQNGPEIIKEIRNQGHEIFLDLKL
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEE
HDIPNTVGQAMKGLGKLDIQMVNVHAAGGSIMMQRALEGLRDSGSTASLIAVTQLTSTSE
CCCCHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCHHH
LMMQQEQNIQSSINDSILSYATLAQKSGLDGVVCSANESAMIRDALGASFLKVTPGIRTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHCCHHEEECCCCEEC
TDDKGDQVRVATPESAAENGSTHIVVGRSITNDANPVEKYRQIKKAWESNVK
CCCCCCEEEEECCCCHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA