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The map label for this gene is pyrK [H]

Identifier: 222151023

GI number: 222151023

Start: 835708

End: 836454

Strand: Direct

Name: pyrK [H]

Synonym: MCCL_0774

Alternate gene names: 222151023

Gene position: 835708-836454 (Clockwise)

Preceding gene: 222151022

Following gene: 222151024

Centisome position: 39.75

GC content: 42.44

Gene sequence:

>747_bases
ATGCGAGTCGTTAAACAAACAAGTATCGCAAGAAATATATATGAAATCGTTTTACAAGGTGGCATTACAAAAAATATGAC
AAAGCCGGGACAATTTGTGCATATGCGTGTTGGCAATAGTTCAGAGTTTATGCTGAGACGTCCAATTTCTATCTGCTCAG
TAGATCAACAAGCACAGACTTTAACGTGTTTATATCGTGCTGAAGGAAAAGGTACAACGCACTTATCACAACTGAAATCA
GGAGATACAGTAGATATATTAGCTCCTCTTGGTAATGGTTTTCCGGTGGAAGCTGCACAGAAAACAGCATTACTCATCGG
TGGAGGTATCGGTGTACCACCATTATATGAATTATCTAAGCAACTCAATGCACGTGGTATTAAAACTATCCACGTGCTCG
GCTTTCAGTCGGCTGAAGATATGTTTTATATTAAACAGTTTGAAGCGCTTGGTACGACGCATGTTGCGACAGTTGATGGG
ACTTATGGTACGAAAGGCTTTGTAACAGACGTTGTAAATGCGATACCGGTCGACTATGATACGTTCTACACATGCGGTCC
GATCGTCATGATTAAGGCGATTCAGGAAACTCTACCGAACACGCCTGGATATGTTTCATTAGAAGAGCGTATGGGATGCG
GTATCGGGGCATGTTATGCATGTGTATGTGAAGCGGATAATGAAGATGGCTACGTGAAGATTTGTACGGATGGCCCAGTA
TTTGAGAAAGGGGCGCTGGCACTATGA

Upstream 100 bases:

>100_bases
GTCTAACATCTCTTGATACAGCACGAGCATTACTTGAAGTCATTGAAAGCATGACATTTAATATGAATCAAATGTAGGGG
GTCTATTGATGCAGGAAGAG

Downstream 100 bases:

>100_bases
GATTAAATGTTGAATTGCCAGGTTTAAAATTAAAAAATCCAGTAATGCCTGCAAGTGGTTGTTTTGCTTTCGGTAAGGAG
TATGCCCAGTTTATGGACTT

Product: dihydroorotate dehydrogenase electron transfer subunit

Products: orotate; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRVVKQTSIARNIYEIVLQGGITKNMTKPGQFVHMRVGNSSEFMLRRPISICSVDQQAQTLTCLYRAEGKGTTHLSQLKS
GDTVDILAPLGNGFPVEAAQKTALLIGGGIGVPPLYELSKQLNARGIKTIHVLGFQSAEDMFYIKQFEALGTTHVATVDG
TYGTKGFVTDVVNAIPVDYDTFYTCGPIVMIKAIQETLPNTPGYVSLEERMGCGIGACYACVCEADNEDGYVKICTDGPV
FEKGALAL

Sequences:

>Translated_248_residues
MRVVKQTSIARNIYEIVLQGGITKNMTKPGQFVHMRVGNSSEFMLRRPISICSVDQQAQTLTCLYRAEGKGTTHLSQLKS
GDTVDILAPLGNGFPVEAAQKTALLIGGGIGVPPLYELSKQLNARGIKTIHVLGFQSAEDMFYIKQFEALGTTHVATVDG
TYGTKGFVTDVVNAIPVDYDTFYTCGPIVMIKAIQETLPNTPGYVSLEERMGCGIGACYACVCEADNEDGYVKICTDGPV
FEKGALAL
>Mature_248_residues
MRVVKQTSIARNIYEIVLQGGITKNMTKPGQFVHMRVGNSSEFMLRRPISICSVDQQAQTLTCLYRAEGKGTTHLSQLKS
GDTVDILAPLGNGFPVEAAQKTALLIGGGIGVPPLYELSKQLNARGIKTIHVLGFQSAEDMFYIKQFEALGTTHVATVDG
TYGTKGFVTDVVNAIPVDYDTFYTCGPIVMIKAIQETLPNTPGYVSLEERMGCGIGACYACVCEADNEDGYVKICTDGPV
FEKGALAL

Specific function: Is responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the pyrD subunit to the ultimate electron acceptor NAD(+) [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.3.3.1

Molecular weight: Translated: 26728; Mature: 26728

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVKQTSIARNIYEIVLQGGITKNMTKPGQFVHMRVGNSSEFMLRRPISICSVDQQAQT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHCCCCCHHHE
LTCLYRAEGKGTTHLSQLKSGDTVDILAPLGNGFPVEAAQKTALLIGGGIGVPPLYELSK
EEEEEEECCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
QLNARGIKTIHVLGFQSAEDMFYIKQFEALGTTHVATVDGTYGTKGFVTDVVNAIPVDYD
HHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCC
TFYTCGPIVMIKAIQETLPNTPGYVSLEERMGCGIGACYACVCEADNEDGYVKICTDGPV
CCCCCCHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCEEEEEEECCCCCCEEEEECCCCC
FEKGALAL
CCCCCCCC
>Mature Secondary Structure
MRVVKQTSIARNIYEIVLQGGITKNMTKPGQFVHMRVGNSSEFMLRRPISICSVDQQAQT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHCCCCCHHHE
LTCLYRAEGKGTTHLSQLKSGDTVDILAPLGNGFPVEAAQKTALLIGGGIGVPPLYELSK
EEEEEEECCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
QLNARGIKTIHVLGFQSAEDMFYIKQFEALGTTHVATVDGTYGTKGFVTDVVNAIPVDYD
HHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCC
TFYTCGPIVMIKAIQETLPNTPGYVSLEERMGCGIGACYACVCEADNEDGYVKICTDGPV
CCCCCCHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCEEEEEEECCCCCCEEEEECCCCC
FEKGALAL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; O2

Specific reaction: (S)-dihydroorotate + O2 = orotate + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA