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The map label for this gene is potD [H]

Identifier: 222150968

GI number: 222150968

Start: 774366

End: 775439

Strand: Direct

Name: potD [H]

Synonym: MCCL_0718

Alternate gene names: 222150968

Gene position: 774366-775439 (Clockwise)

Preceding gene: 222150967

Following gene: 222150969

Centisome position: 36.83

GC content: 39.01

Gene sequence:

>1074_bases
ATGAAACAGCTGATGCAGTTAATCTTCATCTCACTTGCAGTTGCCGTATTGTTGTTATTCTCAAGCCGCTTCATCAATCC
ACCGCCATCAGGTGGAAAAGGGAATGTCCTCTATGTATATAACTGGGGTGAATATATTGATCCTGATCTGCTTAAAAAGT
TTGAGAAGGAAAAAGGCATTCGTGTCGTATTAGAGACGTTTGATTCCAATGAAGCGATGCTCGCAAAGATAAAGAATGGA
GGGACAAGCTATGATATTGCTGTCCCTAGTGAATATGCGATTCAGAAGATGAAAAATGAAAAATTATTGCTGCCCATCGA
TCATAATAAAATACCGAATCTGAAAAATATTAATGAAGATTTTATGGATCTTCCTTTTGATAAGAAGAATAAATACTCTA
TCCCATATTTCTGGGGTACGGTAGGCATTCTATATAATCCCGATACAACGAAAGGCATTGATTTTTCGAGCTGGGATAGT
TTATGGGATAAGCGTCTGAAAAACGATGTCCTTATTGTTGACGGTGCTCGTGAAGCGCTTGGATTAGCGCTGAACAGTAT
GGGTGAGTCTTTAAATGAGACTGACCCTGATAAACTCCTTAAGGCAGAGCAGAAGCTTGAGAAACTTGCACCGAACGTGA
AAGGTGTTGTAGCAGATGAAATTAATACGATGATGGTACAAAAAGAAGCGGATGTTGCGGTTGTATGGAGTGGCATGGGT
GCTGATATCATGACGGAGAACGAAAATCTGGACTTTGTTGTGCCAAAAGAAGGATCGAATCTATGGTTCGACAATATCGT
TATCCCGAAAACGGCACAGAATATTGAAGGGGCGCATGAATTTATTAACTTTCTGCTTGATGCACAAAATGCAAAACAGA
ATACTGAATGGGTTGGATATGCGACACCAAATAAAGCGGCTCTAAAATTACTGGATAAAGAAACACGAGAAGATGAACGA
TTCTATCCGGATAAAGCAGTGCAGGAGAAGCTTGAAGTATATGAAGACCTTGGTTATGAAAATATAAAAAGATATAACGA
ACTCTTCCTTAAATTTAAGATGTCTCTGAAGTAA

Upstream 100 bases:

>100_bases
TCAGCACATTAATGTTCTTAGTCGTGATGACGATCATTGCAATTTACTACGTATTAACAACAAATGCAGATAAAAAGAAG
TTAAAGGCAGGTGCAAAATC

Downstream 100 bases:

>100_bases
GCGTACGTTGATGCAACGATTTTCTATATTTATGCATAAAATCTATTTGTGTGGGTACTATATAAGAAATATGTTTAAAG
GAGATGAATATATGAGTCGT

Product: spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKQLMQLIFISLAVAVLLLFSSRFINPPPSGGKGNVLYVYNWGEYIDPDLLKKFEKEKGIRVVLETFDSNEAMLAKIKNG
GTSYDIAVPSEYAIQKMKNEKLLLPIDHNKIPNLKNINEDFMDLPFDKKNKYSIPYFWGTVGILYNPDTTKGIDFSSWDS
LWDKRLKNDVLIVDGAREALGLALNSMGESLNETDPDKLLKAEQKLEKLAPNVKGVVADEINTMMVQKEADVAVVWSGMG
ADIMTENENLDFVVPKEGSNLWFDNIVIPKTAQNIEGAHEFINFLLDAQNAKQNTEWVGYATPNKAALKLLDKETREDER
FYPDKAVQEKLEVYEDLGYENIKRYNELFLKFKMSLK

Sequences:

>Translated_357_residues
MKQLMQLIFISLAVAVLLLFSSRFINPPPSGGKGNVLYVYNWGEYIDPDLLKKFEKEKGIRVVLETFDSNEAMLAKIKNG
GTSYDIAVPSEYAIQKMKNEKLLLPIDHNKIPNLKNINEDFMDLPFDKKNKYSIPYFWGTVGILYNPDTTKGIDFSSWDS
LWDKRLKNDVLIVDGAREALGLALNSMGESLNETDPDKLLKAEQKLEKLAPNVKGVVADEINTMMVQKEADVAVVWSGMG
ADIMTENENLDFVVPKEGSNLWFDNIVIPKTAQNIEGAHEFINFLLDAQNAKQNTEWVGYATPNKAALKLLDKETREDER
FYPDKAVQEKLEVYEDLGYENIKRYNELFLKFKMSLK
>Mature_357_residues
MKQLMQLIFISLAVAVLLLFSSRFINPPPSGGKGNVLYVYNWGEYIDPDLLKKFEKEKGIRVVLETFDSNEAMLAKIKNG
GTSYDIAVPSEYAIQKMKNEKLLLPIDHNKIPNLKNINEDFMDLPFDKKNKYSIPYFWGTVGILYNPDTTKGIDFSSWDS
LWDKRLKNDVLIVDGAREALGLALNSMGESLNETDPDKLLKAEQKLEKLAPNVKGVVADEINTMMVQKEADVAVVWSGMG
ADIMTENENLDFVVPKEGSNLWFDNIVIPKTAQNIEGAHEFINFLLDAQNAKQNTEWVGYATPNKAALKLLDKETREDER
FYPDKAVQEKLEVYEDLGYENIKRYNELFLKFKMSLK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=320, Percent_Identity=40.3125, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1787078, Length=315, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40668; Mature: 40668

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLMQLIFISLAVAVLLLFSSRFINPPPSGGKGNVLYVYNWGEYIDPDLLKKFEKEKGI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCC
RVVLETFDSNEAMLAKIKNGGTSYDIAVPSEYAIQKMKNEKLLLPIDHNKIPNLKNINED
EEEEEECCCCCEEEEEECCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHH
FMDLPFDKKNKYSIPYFWGTVGILYNPDTTKGIDFSSWDSLWDKRLKNDVLIVDGAREAL
HHCCCCCCCCCCCCCEEECCEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHH
GLALNSMGESLNETDPDKLLKAEQKLEKLAPNVKGVVADEINTMMVQKEADVAVVWSGMG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHEEECCCCEEEEEECCC
ADIMTENENLDFVVPKEGSNLWFDNIVIPKTAQNIEGAHEFINFLLDAQNAKQNTEWVGY
CCEEECCCCCCEEEECCCCCEEECCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
ATPNKAALKLLDKETREDERFYPDKAVQEKLEVYEDLGYENIKRYNELFLKFKMSLK
ECCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MKQLMQLIFISLAVAVLLLFSSRFINPPPSGGKGNVLYVYNWGEYIDPDLLKKFEKEKGI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCC
RVVLETFDSNEAMLAKIKNGGTSYDIAVPSEYAIQKMKNEKLLLPIDHNKIPNLKNINED
EEEEEECCCCCEEEEEECCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHH
FMDLPFDKKNKYSIPYFWGTVGILYNPDTTKGIDFSSWDSLWDKRLKNDVLIVDGAREAL
HHCCCCCCCCCCCCCEEECCEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHH
GLALNSMGESLNETDPDKLLKAEQKLEKLAPNVKGVVADEINTMMVQKEADVAVVWSGMG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHEEECCCCEEEEEECCC
ADIMTENENLDFVVPKEGSNLWFDNIVIPKTAQNIEGAHEFINFLLDAQNAKQNTEWVGY
CCEEECCCCCCEEEECCCCCEEECCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
ATPNKAALKLLDKETREDERFYPDKAVQEKLEVYEDLGYENIKRYNELFLKFKMSLK
ECCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]