Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 222150966

GI number: 222150966

Start: 772757

End: 773563

Strand: Direct

Name: potB [H]

Synonym: MCCL_0716

Alternate gene names: 222150966

Gene position: 772757-773563 (Clockwise)

Preceding gene: 222150965

Following gene: 222150967

Centisome position: 36.76

GC content: 39.16

Gene sequence:

>807_bases
ATGCAAAGAGCGAAAAACTTTTTTTTAGTCCCTTACATGCTCTGGATTATTCTGTTTATCGTAATGCCGCTCATCATGAT
ATTTTATACGTCGTTTACGAATCACGATGGGCAGTTTACATTTGAGAACTACCAGTCTTTTTTGACGCCGACATATTTAA
AGATGACACTCTCTTCCTTTATTTATGCAACGATTATTACCTTATTTTGCTTAATTATTGCGTATCCCATTGCACTCGTC
TTAAAGCAGGCGAAGCATAAGCAGCTATGGTTGCTGATCATTATCTTACCGACGTGGATCAATCTGTTATTAAAGACATA
TGCGTTCATCGGTATATTCAGTATACAAGGCACGCTCAATGATGTGCTGCACTTCTTACATTTACCTGCAGCAAATCTAC
TGTTTACAGATACAGCATTTATCTTTGTATCTGTATATATCTATATACCATTTATGATACTGCCAATCTTTAACAGCATG
AATGAAATTCCGGATAATTTAATCCAGGCGTCACGCGATCTCGGTGCATCCGAGTGGATGACCTTTCGTAAGGTCATCAT
GCCCTTATCACTTGAAGGGGTGAAAACAGGGATACAAGTTACATTTATCCCGGCGTTAAGCTTGTTCATGATTACGCGTC
TAATTGCAGGGAATAAGGTTGTCAACCTGGGGACTGCCATTGAAGAACAGTTTTTAGTGACACAGAACTACGGGATGGGC
TCGACGATTGCCGTATTCTTAATTTTAGCGATGGCATTCGTGATGATCATCACGAAATCTAAAAACGAAAGCGAGGATAT
AAAATGA

Upstream 100 bases:

>100_bases
CGTGTAGGACTTACATTTACTGAGGAAGCGATACATATTATGCTGCCTGGTGAGACAGAAGAAGAATTCGATCGTCGTAT
TGAATCATACGAGGTGTAGT

Downstream 100 bases:

>100_bases
AACTAAGAGGTAAATTGTTTTTAGCGGTCGCACTTTTCATTTTATATTTCCCAATTTTCTATTTGATGTTCTATTCCTTT
AATTCTGCTGGCAATATGAA

Product: spermidine/putrescine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQRAKNFFLVPYMLWIILFIVMPLIMIFYTSFTNHDGQFTFENYQSFLTPTYLKMTLSSFIYATIITLFCLIIAYPIALV
LKQAKHKQLWLLIIILPTWINLLLKTYAFIGIFSIQGTLNDVLHFLHLPAANLLFTDTAFIFVSVYIYIPFMILPIFNSM
NEIPDNLIQASRDLGASEWMTFRKVIMPLSLEGVKTGIQVTFIPALSLFMITRLIAGNKVVNLGTAIEEQFLVTQNYGMG
STIAVFLILAMAFVMIITKSKNESEDIK

Sequences:

>Translated_268_residues
MQRAKNFFLVPYMLWIILFIVMPLIMIFYTSFTNHDGQFTFENYQSFLTPTYLKMTLSSFIYATIITLFCLIIAYPIALV
LKQAKHKQLWLLIIILPTWINLLLKTYAFIGIFSIQGTLNDVLHFLHLPAANLLFTDTAFIFVSVYIYIPFMILPIFNSM
NEIPDNLIQASRDLGASEWMTFRKVIMPLSLEGVKTGIQVTFIPALSLFMITRLIAGNKVVNLGTAIEEQFLVTQNYGMG
STIAVFLILAMAFVMIITKSKNESEDIK
>Mature_268_residues
MQRAKNFFLVPYMLWIILFIVMPLIMIFYTSFTNHDGQFTFENYQSFLTPTYLKMTLSSFIYATIITLFCLIIAYPIALV
LKQAKHKQLWLLIIILPTWINLLLKTYAFIGIFSIQGTLNDVLHFLHLPAANLLFTDTAFIFVSVYIYIPFMILPIFNSM
NEIPDNLIQASRDLGASEWMTFRKVIMPLSLEGVKTGIQVTFIPALSLFMITRLIAGNKVVNLGTAIEEQFLVTQNYGMG
STIAVFLILAMAFVMIITKSKNESEDIK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=253, Percent_Identity=34.3873517786561, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1787080, Length=239, Percent_Identity=33.4728033472803, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787713, Length=238, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30660; Mature: 30660

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]