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The map label for this gene is pdhC [H]

Identifier: 222150962

GI number: 222150962

Start: 767985

End: 769268

Strand: Direct

Name: pdhC [H]

Synonym: MCCL_0712

Alternate gene names: 222150962

Gene position: 767985-769268 (Clockwise)

Preceding gene: 222150961

Following gene: 222150963

Centisome position: 36.53

GC content: 41.04

Gene sequence:

>1284_bases
GTGGCATTTGAATTTAAATTACCTGCCCTTGGTGAAGGGATATTTGAAGGTGAAATCGTAAAGTGGTTCGTTAAGTCTGG
AGACGAAGTACAAGAAGACGATATTTTATTAGAAGTACAAAGTGATAAATCTGTAGTAGAAATTCCATCTCCAGTAACAG
GTAAGATCAATACGATCGTGGCTGAAGAAGGAACTGTAGCAAATCTAGGTGAAGTTATCGTAACAATCGATTCTGACGAT
GCACACGCTCAAAACGACGCTTCTGAAGCGAAAGAAGAGCCAAAAGAAGAAGCGAAAGAAACAAAAGAAGAAGCGCCTAA
AGCACAAGCACCTGCGCAAGACGTTGAAGTTGACGAAAACCGTCGTGTTATCGCAATGCCTTCAGTACGTAAATTAGCCC
GTGATAAAGGGATTAACATTAAAGCTGTTCAAGGTACTGGTAAAAACGGTCGTATCTTAAAAGATGACGTTCTAGCGTAT
GCTGAAGGTGGACAAACAGCTGCAAGTACACCTGAAGCACCAGCACAAGAAGCACCAGCACAAGAGGCAGCACCACAACC
AGTTGCAGCACCAGAAGGTGACTTCCCAGAAACACGCGAGAAAATCCCAGCAATGCGTCGTGCAATTGCAAAAGCAATGG
TGAACTCTAAACATACTGCACCACATGTAACGTTAATGGACGAAGTAGAAGTACAGGCATTATGGGATCACCGTAAGAAG
TTTAAAGAAGTTGCAGCAGAACAAGGTACGAAGTTAACATTCTTACCATACGTTGTAAAAGCATTAGTTTCAGCACTTAA
AGCGTATCCAGCTTTAAACACTTCATTAGATGATGCGACTGAGGAAATCGTCCACAAACATTATTACAACATTGGTATCG
CTGCTGATACTGAACGTGGTCTACTCGTACCTGTTGTAAAAAATGCTGACCGTAAATCAATCTTTGCAATTTCTGATGAA
ATCAATGAATTAGCAGTTAAAGCACGTGACGGCAAACTTTCTCCTAGCGAAATGAAGGGTGCTTCATGTACAATAAGTAA
TATCGGTTCAGCTGGTGGACAATGGTTCACTCCAGTTATTAACCATCCTGAAGTTGCAATTTTAGGTATTGGTCGTATCG
CGCAAAAACCAATCGTTAAAGATGGAGAAATCGTTGCAGCACCAGTTCTTGCATTATCATTAAGTTTCGACCACCGTCAA
ATCGATGGCGCTACTGGACAAAATGCAATGAACCATATTAAACGCTTGTTAAATAATCCAGAATTATTATTAATGGAGGG
GTAA

Upstream 100 bases:

>100_bases
ATTCTAATCTAGAAGATAAACGTGTAAAATGAAAAGTGACATCACGATGTTACTTTTCATTCATTTATTTTAATTCTTAA
TTCTTTAGGAGGAATTTATT

Downstream 100 bases:

>100_bases
ACAGATGGTAGTTGGAGATTTTCCTATTGAAACAGATACTATTGTAATTGGAGCAGGTCCTGGTGGATATGTTGCAGCAA
TTCGTGCAGCACAATTAGGA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAFEFKLPALGEGIFEGEIVKWFVKSGDEVQEDDILLEVQSDKSVVEIPSPVTGKINTIVAEEGTVANLGEVIVTIDSDD
AHAQNDASEAKEEPKEEAKETKEEAPKAQAPAQDVEVDENRRVIAMPSVRKLARDKGINIKAVQGTGKNGRILKDDVLAY
AEGGQTAASTPEAPAQEAPAQEAAPQPVAAPEGDFPETREKIPAMRRAIAKAMVNSKHTAPHVTLMDEVEVQALWDHRKK
FKEVAAEQGTKLTFLPYVVKALVSALKAYPALNTSLDDATEEIVHKHYYNIGIAADTERGLLVPVVKNADRKSIFAISDE
INELAVKARDGKLSPSEMKGASCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQ
IDGATGQNAMNHIKRLLNNPELLLMEG

Sequences:

>Translated_427_residues
MAFEFKLPALGEGIFEGEIVKWFVKSGDEVQEDDILLEVQSDKSVVEIPSPVTGKINTIVAEEGTVANLGEVIVTIDSDD
AHAQNDASEAKEEPKEEAKETKEEAPKAQAPAQDVEVDENRRVIAMPSVRKLARDKGINIKAVQGTGKNGRILKDDVLAY
AEGGQTAASTPEAPAQEAPAQEAAPQPVAAPEGDFPETREKIPAMRRAIAKAMVNSKHTAPHVTLMDEVEVQALWDHRKK
FKEVAAEQGTKLTFLPYVVKALVSALKAYPALNTSLDDATEEIVHKHYYNIGIAADTERGLLVPVVKNADRKSIFAISDE
INELAVKARDGKLSPSEMKGASCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQ
IDGATGQNAMNHIKRLLNNPELLLMEG
>Mature_426_residues
AFEFKLPALGEGIFEGEIVKWFVKSGDEVQEDDILLEVQSDKSVVEIPSPVTGKINTIVAEEGTVANLGEVIVTIDSDDA
HAQNDASEAKEEPKEEAKETKEEAPKAQAPAQDVEVDENRRVIAMPSVRKLARDKGINIKAVQGTGKNGRILKDDVLAYA
EGGQTAASTPEAPAQEAPAQEAAPQPVAAPEGDFPETREKIPAMRRAIAKAMVNSKHTAPHVTLMDEVEVQALWDHRKKF
KEVAAEQGTKLTFLPYVVKALVSALKAYPALNTSLDDATEEIVHKHYYNIGIAADTERGLLVPVVKNADRKSIFAISDEI
NELAVKARDGKLSPSEMKGASCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQI
DGATGQNAMNHIKRLLNNPELLLMEG

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=36.7205542725173, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=29.9319727891156, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI19923748, Length=244, Percent_Identity=34.8360655737705, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI203098753, Length=446, Percent_Identity=28.6995515695067, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI203098816, Length=446, Percent_Identity=28.6995515695067, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=34.8101265822785, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786946, Length=428, Percent_Identity=30.607476635514, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=33.4894613583138, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=33.3333333333333, Blast_Score=247, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=30.4250559284116, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=419, Percent_Identity=30.3102625298329, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=30.5466237942122, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=31.5165876777251, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=29.2035398230088, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321632, Length=175, Percent_Identity=32, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=36.5339578454333, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=33.4763948497854, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=32.618025751073, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=32.618025751073, Blast_Score=128, Evalue=8e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46028; Mature: 45896

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFKLPALGEGIFEGEIVKWFVKSGDEVQEDDILLEVQSDKSVVEIPSPVTGKINTIV
CCEEECCCHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEE
AEEGTVANLGEVIVTIDSDDAHAQNDASEAKEEPKEEAKETKEEAPKAQAPAQDVEVDEN
ECCCCEECCCEEEEEECCCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCC
RRVIAMPSVRKLARDKGINIKAVQGTGKNGRILKDDVLAYAEGGQTAASTPEAPAQEAPA
CEEEECCHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHEEECCCCCCCCCCCCCCCCCCH
QEAAPQPVAAPEGDFPETREKIPAMRRAIAKAMVNSKHTAPHVTLMDEVEVQALWDHRKK
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHH
FKEVAAEQGTKLTFLPYVVKALVSALKAYPALNTSLDDATEEIVHKHYYNIGIAADTERG
HHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCC
LLVPVVKNADRKSIFAISDEINELAVKARDGKLSPSEMKGASCTISNIGSAGGQWFTPVI
EEEEEECCCCCCEEEEEHHHHHHHEEEECCCCCCHHHCCCCEEEHHHCCCCCCCEECCCC
NHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQIDGATGQNAMNHIKRLLNNP
CCCCEEEEECCHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
ELLLMEG
CEEEECC
>Mature Secondary Structure 
AFEFKLPALGEGIFEGEIVKWFVKSGDEVQEDDILLEVQSDKSVVEIPSPVTGKINTIV
CEEECCCHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEE
AEEGTVANLGEVIVTIDSDDAHAQNDASEAKEEPKEEAKETKEEAPKAQAPAQDVEVDEN
ECCCCEECCCEEEEEECCCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCC
RRVIAMPSVRKLARDKGINIKAVQGTGKNGRILKDDVLAYAEGGQTAASTPEAPAQEAPA
CEEEECCHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHEEECCCCCCCCCCCCCCCCCCH
QEAAPQPVAAPEGDFPETREKIPAMRRAIAKAMVNSKHTAPHVTLMDEVEVQALWDHRKK
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHH
FKEVAAEQGTKLTFLPYVVKALVSALKAYPALNTSLDDATEEIVHKHYYNIGIAADTERG
HHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCC
LLVPVVKNADRKSIFAISDEINELAVKARDGKLSPSEMKGASCTISNIGSAGGQWFTPVI
EEEEEECCCCCCEEEEEHHHHHHHEEEECCCCCCHHHCCCCEEEHHHCCCCCCCEECCCC
NHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQIDGATGQNAMNHIKRLLNNP
CCCCEEEEECCHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
ELLLMEG
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA