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The map label for this gene is ptsI [H]

Identifier: 222150951

GI number: 222150951

Start: 755043

End: 756752

Strand: Direct

Name: ptsI [H]

Synonym: MCCL_0701

Alternate gene names: 222150951

Gene position: 755043-756752 (Clockwise)

Preceding gene: 222150950

Following gene: 222150953

Centisome position: 35.91

GC content: 41.93

Gene sequence:

>1710_bases
ATGACGACTTTAAAAGGTATCGGTGCATCAGATGGCATCGCAATTGCCAAAGCATACCTTCTGGTTGAACCTGATCTGTC
TTTTAACAATGATAAAGTAGCAGATGTAGATGCAGAAGTTGAGAAATTCAAAGCTGCCATCAATCAATCTAAGGTAGAGT
TGACACAGATTCGTAACAATGCAGAAATAGCGCTTGGCCCAGATAAGGCAGCAATCTTTGATGCGCATCTGCTTGTCCTT
GAAGACCCTGAGCTAATCAACCCAATTGAAGATAACATTCGAAATAATCAGATGAATGCAGCCGCAAGTCTCTCTGAAGT
TTCAACGAATTTCATTACAATATTTGAATCGATGGATAATGAATATATGAAAGAACGTGCAGCAGATATTAAAGATGTAT
CTAAACGCGTATTATCGCATATTCTAGGTGTGTCACTACCAAATCCTACGATGATTGATGAGCCCGTTGTCATCATCGCA
GAAGACTTAACGCCTTCTGATACTGCGCAACTGAACAAGAAGTTCGTTCAAGGATTTGCGACAAATATCGGTGGGCGTAC
GAGCCACTCAGCAATTATGAGCCGTTCATTAGAAATTCCGGCGGTTGTTGGGACTAAAAACATTACTGATGCTGTACAGC
AAGGAGATTTTGTAATTGTTGACGGTATTGCAGGAGAAGTCATCATCAATCCAGATGAGCAGACGGTTACGGGATATAAA
GCTCGTCAAGCTGCTTTCCTGCAGGAGAAAGAAGAATTAAAGCAGCTGGTTAACGATAAGACAGTCACTAAAGAAGGTAA
GCACGTAGAGCTTGCTGCGAATATCGGTACACCGAATGATCTTGAAGGTGTTAAGAATAACGGTGCAGAAGGTATCGGCC
TCTATCGTACAGAGTTTCTTTATATGGGACGCGATGCAATGCCTTCTGAAGATGAACAGTTTGAAGCTTATAAGAAAGTA
TTATCTGAGATGGATGGTAAACGTGTTGTTGTCCGTACATTAGATATCGGTGGAGATAAGGAACTTCCGTACTTAAACTT
ACCAAAAGAGATGAACCCGTTCCTTGGATACCGTGCGATTCGACTTTGTCTGGATCAGCAGGATATCTTTAGAACACAGC
TTCGTGCGTTACTACGTGCATCCATCCATGGTAAACTGAGCATCATGTTCCCGATGATTGCGACTATCAACGAATTCCGT
GAGGCGAAAGCAATCTTAGAAGAAGAGAAAGCCAATTTAATTGCAGATAAAGTTAAAGTTGCTGACGATATCGAGCTTGG
CATCATGGTAGAGATTCCTTCTACAGCAGCAATGGCCGATATCTTTGCGAAAGAGGTTGATTTCTTTAGTATCGGTACGA
ATGATCTTATTCAATATACGATGGCAGCAGACCGTATGAGTGAACGCGTCTCTTACCTTTACCAGCCTTATAACCCAGCG
ATTCTTAGACTGATCAAACAAGTTATTGATGCCAGTCATAAAGAAGGTAAATGGACAGGGATGTGTGGAGAAATGGCAGG
AGATTCGACAGCAATTCCGTTATTGCTAGGTCTCGGCCTTGATGAGTTCAGTATGAGTGCGACAAGTATCTTAAGTGCAC
GTCGTCAGATTAAGAACTTAAGTCAGCCAGAGATGGCGAAAGTAGCTGAGCAAGCTTTAAACTGTGCAACACAGGAAGAA
GTTGTAAAGCTCGTAGATGCATATCTATAA

Upstream 100 bases:

>100_bases
GTAAAGATGCAGAAATTACAATCTACGCTGAAGGTAGCGATGAGAAAGAAGCAATCGATGCAATCAGCGAAGTATTAGTT
AAAGAAGGTTTAAGCAAATA

Downstream 100 bases:

>100_bases
TCTGAATTAAATTTAAGTTAAATAAATATAACCCGAATGACGGAGAAGATATAATTCATCTTCTCCGTCATTCGGGTTAT
TTGTGCAGTTACTTATTAAT

Product: phosphoenolpyruvate-protein phosphatase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MTTLKGIGASDGIAIAKAYLLVEPDLSFNNDKVADVDAEVEKFKAAINQSKVELTQIRNNAEIALGPDKAAIFDAHLLVL
EDPELINPIEDNIRNNQMNAAASLSEVSTNFITIFESMDNEYMKERAADIKDVSKRVLSHILGVSLPNPTMIDEPVVIIA
EDLTPSDTAQLNKKFVQGFATNIGGRTSHSAIMSRSLEIPAVVGTKNITDAVQQGDFVIVDGIAGEVIINPDEQTVTGYK
ARQAAFLQEKEELKQLVNDKTVTKEGKHVELAANIGTPNDLEGVKNNGAEGIGLYRTEFLYMGRDAMPSEDEQFEAYKKV
LSEMDGKRVVVRTLDIGGDKELPYLNLPKEMNPFLGYRAIRLCLDQQDIFRTQLRALLRASIHGKLSIMFPMIATINEFR
EAKAILEEEKANLIADKVKVADDIELGIMVEIPSTAAMADIFAKEVDFFSIGTNDLIQYTMAADRMSERVSYLYQPYNPA
ILRLIKQVIDASHKEGKWTGMCGEMAGDSTAIPLLLGLGLDEFSMSATSILSARRQIKNLSQPEMAKVAEQALNCATQEE
VVKLVDAYL

Sequences:

>Translated_569_residues
MTTLKGIGASDGIAIAKAYLLVEPDLSFNNDKVADVDAEVEKFKAAINQSKVELTQIRNNAEIALGPDKAAIFDAHLLVL
EDPELINPIEDNIRNNQMNAAASLSEVSTNFITIFESMDNEYMKERAADIKDVSKRVLSHILGVSLPNPTMIDEPVVIIA
EDLTPSDTAQLNKKFVQGFATNIGGRTSHSAIMSRSLEIPAVVGTKNITDAVQQGDFVIVDGIAGEVIINPDEQTVTGYK
ARQAAFLQEKEELKQLVNDKTVTKEGKHVELAANIGTPNDLEGVKNNGAEGIGLYRTEFLYMGRDAMPSEDEQFEAYKKV
LSEMDGKRVVVRTLDIGGDKELPYLNLPKEMNPFLGYRAIRLCLDQQDIFRTQLRALLRASIHGKLSIMFPMIATINEFR
EAKAILEEEKANLIADKVKVADDIELGIMVEIPSTAAMADIFAKEVDFFSIGTNDLIQYTMAADRMSERVSYLYQPYNPA
ILRLIKQVIDASHKEGKWTGMCGEMAGDSTAIPLLLGLGLDEFSMSATSILSARRQIKNLSQPEMAKVAEQALNCATQEE
VVKLVDAYL
>Mature_568_residues
TTLKGIGASDGIAIAKAYLLVEPDLSFNNDKVADVDAEVEKFKAAINQSKVELTQIRNNAEIALGPDKAAIFDAHLLVLE
DPELINPIEDNIRNNQMNAAASLSEVSTNFITIFESMDNEYMKERAADIKDVSKRVLSHILGVSLPNPTMIDEPVVIIAE
DLTPSDTAQLNKKFVQGFATNIGGRTSHSAIMSRSLEIPAVVGTKNITDAVQQGDFVIVDGIAGEVIINPDEQTVTGYKA
RQAAFLQEKEELKQLVNDKTVTKEGKHVELAANIGTPNDLEGVKNNGAEGIGLYRTEFLYMGRDAMPSEDEQFEAYKKVL
SEMDGKRVVVRTLDIGGDKELPYLNLPKEMNPFLGYRAIRLCLDQQDIFRTQLRALLRASIHGKLSIMFPMIATINEFRE
AKAILEEEKANLIADKVKVADDIELGIMVEIPSTAAMADIFAKEVDFFSIGTNDLIQYTMAADRMSERVSYLYQPYNPAI
LRLIKQVIDASHKEGKWTGMCGEMAGDSTAIPLLLGLGLDEFSMSATSILSARRQIKNLSQPEMAKVAEQALNCATQEEV
VKLVDAYL

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=568, Percent_Identity=49.8239436619718, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1788726, Length=499, Percent_Identity=39.0781563126253, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=38.2470119521912, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI1789193, Length=580, Percent_Identity=32.4137931034483, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1787994, Length=438, Percent_Identity=30.1369863013699, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI226510935, Length=186, Percent_Identity=26.8817204301075, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 62611; Mature: 62480

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLKGIGASDGIAIAKAYLLVEPDLSFNNDKVADVDAEVEKFKAAINQSKVELTQIRNN
CCCCCCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEHHHHCCC
AEIALGPDKAAIFDAHLLVLEDPELINPIEDNIRNNQMNAAASLSEVSTNFITIFESMDN
CEEEECCCCCEEEEEEEEEECCCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EYMKERAADIKDVSKRVLSHILGVSLPNPTMIDEPVVIIAEDLTPSDTAQLNKKFVQGFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TNIGGRTSHSAIMSRSLEIPAVVGTKNITDAVQQGDFVIVDGIAGEVIINPDEQTVTGYK
HCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCHH
ARQAAFLQEKEELKQLVNDKTVTKEGKHVELAANIGTPNDLEGVKNNGAEGIGLYRTEFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCEEHHHHHHE
YMGRDAMPSEDEQFEAYKKVLSEMDGKRVVVRTLDIGGDKELPYLNLPKEMNPFLGYRAI
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEECCCHHCCHHHHHHHH
RLCLDQQDIFRTQLRALLRASIHGKLSIMFPMIATINEFREAKAILEEEKANLIADKVKV
HHHHCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADDIELGIMVEIPSTAAMADIFAKEVDFFSIGTNDLIQYTMAADRMSERVSYLYQPYNPA
CCCCCEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
ILRLIKQVIDASHKEGKWTGMCGEMAGDSTAIPLLLGLGLDEFSMSATSILSARRQIKNL
HHHHHHHHHCCCCCCCCEEECCHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
SQPEMAKVAEQALNCATQEEVVKLVDAYL
CCHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TTLKGIGASDGIAIAKAYLLVEPDLSFNNDKVADVDAEVEKFKAAINQSKVELTQIRNN
CCCCCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEHHHHCCC
AEIALGPDKAAIFDAHLLVLEDPELINPIEDNIRNNQMNAAASLSEVSTNFITIFESMDN
CEEEECCCCCEEEEEEEEEECCCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EYMKERAADIKDVSKRVLSHILGVSLPNPTMIDEPVVIIAEDLTPSDTAQLNKKFVQGFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TNIGGRTSHSAIMSRSLEIPAVVGTKNITDAVQQGDFVIVDGIAGEVIINPDEQTVTGYK
HCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCHH
ARQAAFLQEKEELKQLVNDKTVTKEGKHVELAANIGTPNDLEGVKNNGAEGIGLYRTEFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCEEHHHHHHE
YMGRDAMPSEDEQFEAYKKVLSEMDGKRVVVRTLDIGGDKELPYLNLPKEMNPFLGYRAI
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEECCCHHCCHHHHHHHH
RLCLDQQDIFRTQLRALLRASIHGKLSIMFPMIATINEFREAKAILEEEKANLIADKVKV
HHHHCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADDIELGIMVEIPSTAAMADIFAKEVDFFSIGTNDLIQYTMAADRMSERVSYLYQPYNPA
CCCCCEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
ILRLIKQVIDASHKEGKWTGMCGEMAGDSTAIPLLLGLGLDEFSMSATSILSARRQIKNL
HHHHHHHHHCCCCCCCCEEECCHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
SQPEMAKVAEQALNCATQEEVVKLVDAYL
CCHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1551842; 1901791 [H]