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The map label for this gene is purK [H]

Identifier: 222150938

GI number: 222150938

Start: 740409

End: 741527

Strand: Direct

Name: purK [H]

Synonym: MCCL_0688

Alternate gene names: 222150938

Gene position: 740409-741527 (Clockwise)

Preceding gene: 222150937

Following gene: 222150939

Centisome position: 35.22

GC content: 46.38

Gene sequence:

>1119_bases
ATGACCTTAAATAATTTCGCGCTCGGTGACACGATTGGTATCATCGGCGGGGGTCAGCTGGGTAAGATGATGGCACAGTC
TGCACAGCGCATGGGATTTAAAGTTGCTGTGCTTGATCCCGATGAACGTTGTCCTGCACGCTTCGTGGCACATCACTTCG
TGCATGCACCGTACAATGATATTGCAGGATTGAAAGCGTTAAGCCGCTATAGCGACGTCATCACATATGAATTTGAGAAT
ATTAATTCAGAGGCGCTGGAAGTCATTGTAAAAGAAAATCATGTGCCTCAAGGTTTTGAAACGGTACATATTCTGCAGAA
TCGTCTCAGTGAGAAGGAAGCCATCGCAAGAAGTGGCGCTACGGTTGTTCCATACAGGCGTATCGAAGGTCCGGCGGATG
TCGATGCTGCTGTGCGTGAGATCGGTCTGCCACTCGTACTGAAGACGGTGTTCGGCGGTTATGATGGCAAAGGTCAGCTT
GTTGTAACGGAGAAAGCACAGCTAGAAGAAGCGTACAATATGGCTGATGGCAATGTTCTCGTCGCAGAGAAACGTATTGC
GCTGGACCGTGAGATTTCGATTACAGCATCACGTAATCTTCAGGGAGAGGTCGTCTACTTCCCTGTACAGGAGAATGTCC
ATCGTAACCAGATTCTGTTCAGTACGACGGTGAAGCAGGCGCATGAGTATAGAGCAGCTGCGATTGCTGAAGTGGAGAAG
ATCATTCATAAGATTCATTTCGTCGGTACGTTCACTGTGGAATTCTTTATCGATAAAGCGGGTAAGCTCTACGTCAACGA
AATCGCACCACGTCCTCATAACTCCGGACATTACTCTATTGAAGCGTGTGATTATTCGCAATTTGATACGCATATTCTGT
CGGTGCTTGGCTACAGATTACCGAAACAAATCGAGCTGCACAGTTCTGCTGTGATGTACAATCTGCTCGGTCAGGATCTG
GACTTGATGGAACATCTCTTCCCTGAACATCCAGAATGGCATGTCCATGTATATGGTAAAGAGGCAAGGAAAGAAAACAG
GAAGATGGGACATATTACCCTTTTGTTAGACGATGACACGGATACTTATTATCCTATATATAAGGAGCTGATGAAATGA

Upstream 100 bases:

>100_bases
ATTCTTGCCGCACGTATGCTCAGTCTGAAGGATACCGCTTTAGTAGCTCAGCTGGATGATTATGAAGCATCTTTAGTAAA
GAAAGTGGAGGCGATGCAGC

Downstream 100 bases:

>100_bases
CCTTAGTTTATGAAGGAAAAGCAAAGCGTGTATACAGTACGGATGAGGACCATGTGTTCCGCATCGAATACAAGGATGAA
GTAACAGCGGGCAACGGTGC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTLNNFALGDTIGIIGGGQLGKMMAQSAQRMGFKVAVLDPDERCPARFVAHHFVHAPYNDIAGLKALSRYSDVITYEFEN
INSEALEVIVKENHVPQGFETVHILQNRLSEKEAIARSGATVVPYRRIEGPADVDAAVREIGLPLVLKTVFGGYDGKGQL
VVTEKAQLEEAYNMADGNVLVAEKRIALDREISITASRNLQGEVVYFPVQENVHRNQILFSTTVKQAHEYRAAAIAEVEK
IIHKIHFVGTFTVEFFIDKAGKLYVNEIAPRPHNSGHYSIEACDYSQFDTHILSVLGYRLPKQIELHSSAVMYNLLGQDL
DLMEHLFPEHPEWHVHVYGKEARKENRKMGHITLLLDDDTDTYYPIYKELMK

Sequences:

>Translated_372_residues
MTLNNFALGDTIGIIGGGQLGKMMAQSAQRMGFKVAVLDPDERCPARFVAHHFVHAPYNDIAGLKALSRYSDVITYEFEN
INSEALEVIVKENHVPQGFETVHILQNRLSEKEAIARSGATVVPYRRIEGPADVDAAVREIGLPLVLKTVFGGYDGKGQL
VVTEKAQLEEAYNMADGNVLVAEKRIALDREISITASRNLQGEVVYFPVQENVHRNQILFSTTVKQAHEYRAAAIAEVEK
IIHKIHFVGTFTVEFFIDKAGKLYVNEIAPRPHNSGHYSIEACDYSQFDTHILSVLGYRLPKQIELHSSAVMYNLLGQDL
DLMEHLFPEHPEWHVHVYGKEARKENRKMGHITLLLDDDTDTYYPIYKELMK
>Mature_371_residues
TLNNFALGDTIGIIGGGQLGKMMAQSAQRMGFKVAVLDPDERCPARFVAHHFVHAPYNDIAGLKALSRYSDVITYEFENI
NSEALEVIVKENHVPQGFETVHILQNRLSEKEAIARSGATVVPYRRIEGPADVDAAVREIGLPLVLKTVFGGYDGKGQLV
VTEKAQLEEAYNMADGNVLVAEKRIALDREISITASRNLQGEVVYFPVQENVHRNQILFSTTVKQAHEYRAAAIAEVEKI
IHKIHFVGTFTVEFFIDKAGKLYVNEIAPRPHNSGHYSIEACDYSQFDTHILSVLGYRLPKQIELHSSAVMYNLLGQDLD
LMEHLFPEHPEWHVHVYGKEARKENRKMGHITLLLDDDTDTYYPIYKELMK

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=355, Percent_Identity=28.169014084507, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788155, Length=383, Percent_Identity=27.154046997389, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=32.5136612021858, Blast_Score=190, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006140
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C; PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41945; Mature: 41814

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNNFALGDTIGIIGGGQLGKMMAQSAQRMGFKVAVLDPDERCPARFVAHHFVHAPYND
CCCCCEECCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCHH
IAGLKALSRYSDVITYEFENINSEALEVIVKENHVPQGFETVHILQNRLSEKEAIARSGA
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TVVPYRRIEGPADVDAAVREIGLPLVLKTVFGGYDGKGQLVVTEKAQLEEAYNMADGNVL
EEECHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCEE
VAEKRIALDREISITASRNLQGEVVYFPVQENVHRNQILFSTTVKQAHEYRAAAIAEVEK
EEEHHEECCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
IIHKIHFVGTFTVEFFIDKAGKLYVNEIAPRPHNSGHYSIEACDYSQFDTHILSVLGYRL
HHHHHHHHEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC
PKQIELHSSAVMYNLLGQDLDLMEHLFPEHPEWHVHVYGKEARKENRKMGHITLLLDDDT
CCCEEHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECHHHHHCCCCEEEEEEEEECCC
DTYYPIYKELMK
CCCHHHHHHHCC
>Mature Secondary Structure 
TLNNFALGDTIGIIGGGQLGKMMAQSAQRMGFKVAVLDPDERCPARFVAHHFVHAPYND
CCCCEECCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCHH
IAGLKALSRYSDVITYEFENINSEALEVIVKENHVPQGFETVHILQNRLSEKEAIARSGA
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TVVPYRRIEGPADVDAAVREIGLPLVLKTVFGGYDGKGQLVVTEKAQLEEAYNMADGNVL
EEECHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCEE
VAEKRIALDREISITASRNLQGEVVYFPVQENVHRNQILFSTTVKQAHEYRAAAIAEVEK
EEEHHEECCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
IIHKIHFVGTFTVEFFIDKAGKLYVNEIAPRPHNSGHYSIEACDYSQFDTHILSVLGYRL
HHHHHHHHEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC
PKQIELHSSAVMYNLLGQDLDLMEHLFPEHPEWHVHVYGKEARKENRKMGHITLLLDDDT
CCCEEHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECHHHHHCCCCEEEEEEEEECCC
DTYYPIYKELMK
CCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA