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The map label for this gene is capD [H]

Identifier: 222150920

GI number: 222150920

Start: 722575

End: 724365

Strand: Direct

Name: capD [H]

Synonym: MCCL_0670

Alternate gene names: 222150920

Gene position: 722575-724365 (Clockwise)

Preceding gene: 222150919

Following gene: 222150921

Centisome position: 34.37

GC content: 33.33

Gene sequence:

>1791_bases
ATGACAGCTAAAAAAAGGTTAATGTTCTTAATTTTTTTAGATTCAATCATCGTTTCACTTTCTGTCTTTTTCTGCTATTC
AATTATCTTTCCATTTTTTAAAGGCAATCAGCTCAATGATTTATTAGTATATTCAGTACTTCTATTGCTAAGTCATCATC
TATTTGCATATATATTTAATCTGTATCATCGTGCATGGGAATATGCGAGCATTAATGAACTATTAGCAATCGTAAAAGGC
GTTACCGCAAGTATCATATTAACGTCAATCCTTTCCTTAATTATTAATGGGCAAGTCTTCATCCGATTAATATTTATTAC
CTGGATGATGCACCTTATATTAATTGGTGGTTCAAGAATCAGCTGGCGAGTTTTAAGACGACATATTCTAGATAAAGATA
TTGAATTAATTCCAACCTTGGTTGTAGGTGCGGGTCGTGGAGGAAACTTATTAATTCATCAGATGTTATCAACACCTACA
ATGGAGATGAAACCTGTAGTAGTAGTTGATGATGATATAAACAAGAAGAATATTGAATTAACAGATGGAATCAAGGTGAA
AGGCACAACTGAAGATATACATAGTTTAGTAACAAAATACGATATTAAACGTATTGTCATCGCTATTCCAAGTCTTGATA
AGCAAAAGTTAAATGATATACATAAAGCTGCAACTGAGACGGGGTCAGAAGTTTTAATTATGCCGAATATCGATGATGTG
ATGGCTGGTAAACTTGAAGTTTCACAGCTTAAGAAAGTAGAAGTAGAAGATTTGTTGGGTCGTGATCCTATTGAACTTGA
TGATCAAGGGATTTTAGAACAAATAGAAGGTAAGACTATACTTGTTACAGGTGCTGGAGGCTCTATCGGCTCCGAATTGT
GTCGTCAAATTGTAAAATACAAACCCAGACGTCTTGTAGTGTTAGGACATGGAGAAAATTCGATTTACTTAATCAACGAA
GAATTAAACAATAAGCTGCTGGGTAGTGTTGAAATTATTCCAATAATTGCAGATGTGCAGGATAGAGAGCGTATGTTTAA
ACAAATGTTGAAGTATAAACCGGATATTGTGTATCATGCTGCTGCCCATAAACATGTTCCATTAATGGAATATAATTCAA
CAGAAGCTGTGAAGAATAATATCATTGGAACAAAGAATACTGCAGAGGCTGCTTTAAATGCGAGTGTTAAACGATTTGTA
TTGGTATCTACAGATAAAGCAGTTAATCCGCCAAATGTAATGGGTGCTACAAAAAGAATGGCTGAAATGGTGATACAGGC
CTTAGATACTCAAAGTAAAACTACTAGATTTGTAGCTGTAAGATTTGGAAATGTTTTAGGATCTAGAGGTTCAGTGATAC
CTAAGTTTAAACAGCAAATTGAAGCTGGAGGTCCTGTAACTGTTACACATCCAGATATGACACGTTATTTTATGACTATA
CCAGAAGCCTCTAGATTAGTCATTCAAGCAGGTGCACTTGCAAATGGTGGAGAAATATTTGTACTTGACATGGGTGAACC
TGTTAAAATAGTTAACCTTGCGAAAAATATGATAAAATTAAGCGGTTTCTCTGAAGAAGATATAAAAATTGAATTTTCAG
GTATAAGACCTGGAGAAAAGCTGTTTGAAGAATTATTGGATGCTGATGAAATACATCCTGAACAAATCTATCCTAAAATT
TATATTGGAAAAGCGAATGAAATAAATATTGCTGATTTGGAAAATATTATTCCGAAGATATTAAAATCAAATAACCTTAA
AGAAGATTTATTAAATTTTATTAAAAAGTAG

Upstream 100 bases:

>100_bases
TACTTGGAGTAGTTCTGAACAATGTCGAGAAATCATCAACAGATGACTAATACTATTATGAATACTATGGCAAATCAAAT
TAACAGAAAGGAACAACTTT

Downstream 100 bases:

>100_bases
ATGATGAATTGAGTGGAAAAAATAAGTTCTTAATTTTTGTTGTTGGAAATATTAGTTATGGAGTTTCACAATGGATTATT
TTGTTAGGAATATTAAAGAT

Product: capsular polysaccharide biosynthesis protein CapD

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MTAKKRLMFLIFLDSIIVSLSVFFCYSIIFPFFKGNQLNDLLVYSVLLLLSHHLFAYIFNLYHRAWEYASINELLAIVKG
VTASIILTSILSLIINGQVFIRLIFITWMMHLILIGGSRISWRVLRRHILDKDIELIPTLVVGAGRGGNLLIHQMLSTPT
MEMKPVVVVDDDINKKNIELTDGIKVKGTTEDIHSLVTKYDIKRIVIAIPSLDKQKLNDIHKAATETGSEVLIMPNIDDV
MAGKLEVSQLKKVEVEDLLGRDPIELDDQGILEQIEGKTILVTGAGGSIGSELCRQIVKYKPRRLVVLGHGENSIYLINE
ELNNKLLGSVEIIPIIADVQDRERMFKQMLKYKPDIVYHAAAHKHVPLMEYNSTEAVKNNIIGTKNTAEAALNASVKRFV
LVSTDKAVNPPNVMGATKRMAEMVIQALDTQSKTTRFVAVRFGNVLGSRGSVIPKFKQQIEAGGPVTVTHPDMTRYFMTI
PEASRLVIQAGALANGGEIFVLDMGEPVKIVNLAKNMIKLSGFSEEDIKIEFSGIRPGEKLFEELLDADEIHPEQIYPKI
YIGKANEINIADLENIIPKILKSNNLKEDLLNFIKK

Sequences:

>Translated_596_residues
MTAKKRLMFLIFLDSIIVSLSVFFCYSIIFPFFKGNQLNDLLVYSVLLLLSHHLFAYIFNLYHRAWEYASINELLAIVKG
VTASIILTSILSLIINGQVFIRLIFITWMMHLILIGGSRISWRVLRRHILDKDIELIPTLVVGAGRGGNLLIHQMLSTPT
MEMKPVVVVDDDINKKNIELTDGIKVKGTTEDIHSLVTKYDIKRIVIAIPSLDKQKLNDIHKAATETGSEVLIMPNIDDV
MAGKLEVSQLKKVEVEDLLGRDPIELDDQGILEQIEGKTILVTGAGGSIGSELCRQIVKYKPRRLVVLGHGENSIYLINE
ELNNKLLGSVEIIPIIADVQDRERMFKQMLKYKPDIVYHAAAHKHVPLMEYNSTEAVKNNIIGTKNTAEAALNASVKRFV
LVSTDKAVNPPNVMGATKRMAEMVIQALDTQSKTTRFVAVRFGNVLGSRGSVIPKFKQQIEAGGPVTVTHPDMTRYFMTI
PEASRLVIQAGALANGGEIFVLDMGEPVKIVNLAKNMIKLSGFSEEDIKIEFSGIRPGEKLFEELLDADEIHPEQIYPKI
YIGKANEINIADLENIIPKILKSNNLKEDLLNFIKK
>Mature_595_residues
TAKKRLMFLIFLDSIIVSLSVFFCYSIIFPFFKGNQLNDLLVYSVLLLLSHHLFAYIFNLYHRAWEYASINELLAIVKGV
TASIILTSILSLIINGQVFIRLIFITWMMHLILIGGSRISWRVLRRHILDKDIELIPTLVVGAGRGGNLLIHQMLSTPTM
EMKPVVVVDDDINKKNIELTDGIKVKGTTEDIHSLVTKYDIKRIVIAIPSLDKQKLNDIHKAATETGSEVLIMPNIDDVM
AGKLEVSQLKKVEVEDLLGRDPIELDDQGILEQIEGKTILVTGAGGSIGSELCRQIVKYKPRRLVVLGHGENSIYLINEE
LNNKLLGSVEIIPIIADVQDRERMFKQMLKYKPDIVYHAAAHKHVPLMEYNSTEAVKNNIIGTKNTAEAALNASVKRFVL
VSTDKAVNPPNVMGATKRMAEMVIQALDTQSKTTRFVAVRFGNVLGSRGSVIPKFKQQIEAGGPVTVTHPDMTRYFMTIP
EASRLVIQAGALANGGEIFVLDMGEPVKIVNLAKNMIKLSGFSEEDIKIEFSGIRPGEKLFEELLDADEIHPEQIYPKIY
IGKANEINIADLENIIPKILKSNNLKEDLLNFIKK

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 66640; Mature: 66509

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKKRLMFLIFLDSIIVSLSVFFCYSIIFPFFKGNQLNDLLVYSVLLLLSHHLFAYIFN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LYHRAWEYASINELLAIVKGVTASIILTSILSLIINGQVFIRLIFITWMMHLILIGGSRI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCH
SWRVLRRHILDKDIELIPTLVVGAGRGGNLLIHQMLSTPTMEMKPVVVVDDDINKKNIEL
HHHHHHHHHHCCCHHHHHHHEEECCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCEEE
TDGIKVKGTTEDIHSLVTKYDIKRIVIAIPSLDKQKLNDIHKAATETGSEVLIMPNIDDV
ECCEEECCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHH
MAGKLEVSQLKKVEVEDLLGRDPIELDDQGILEQIEGKTILVTGAGGSIGSELCRQIVKY
HHCCHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHC
KPRRLVVLGHGENSIYLINEELNNKLLGSVEIIPIIADVQDRERMFKQMLKYKPDIVYHA
CCCEEEEEECCCCEEEEECCCCCCHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEE
AAHKHVPLMEYNSTEAVKNNIIGTKNTAEAALNASVKRFVLVSTDKAVNPPNVMGATKRM
CCCCCCCEEECCCHHHHHHCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHH
AEMVIQALDTQSKTTRFVAVRFGNVLGSRGSVIPKFKQQIEAGGPVTVTHPDMTRYFMTI
HHHHHHHHCCCCCCEEEEEEEEHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCEEEEEEC
PEASRLVIQAGALANGGEIFVLDMGEPVKIVNLAKNMIKLSGFSEEDIKIEFSGIRPGEK
CCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHH
LFEELLDADEIHPEQIYPKIYIGKANEINIADLENIIPKILKSNNLKEDLLNFIKK
HHHHHHCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TAKKRLMFLIFLDSIIVSLSVFFCYSIIFPFFKGNQLNDLLVYSVLLLLSHHLFAYIFN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LYHRAWEYASINELLAIVKGVTASIILTSILSLIINGQVFIRLIFITWMMHLILIGGSRI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCH
SWRVLRRHILDKDIELIPTLVVGAGRGGNLLIHQMLSTPTMEMKPVVVVDDDINKKNIEL
HHHHHHHHHHCCCHHHHHHHEEECCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCEEE
TDGIKVKGTTEDIHSLVTKYDIKRIVIAIPSLDKQKLNDIHKAATETGSEVLIMPNIDDV
ECCEEECCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHH
MAGKLEVSQLKKVEVEDLLGRDPIELDDQGILEQIEGKTILVTGAGGSIGSELCRQIVKY
HHCCHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHC
KPRRLVVLGHGENSIYLINEELNNKLLGSVEIIPIIADVQDRERMFKQMLKYKPDIVYHA
CCCEEEEEECCCCEEEEECCCCCCHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEE
AAHKHVPLMEYNSTEAVKNNIIGTKNTAEAALNASVKRFVLVSTDKAVNPPNVMGATKRM
CCCCCCCEEECCCHHHHHHCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHH
AEMVIQALDTQSKTTRFVAVRFGNVLGSRGSVIPKFKQQIEAGGPVTVTHPDMTRYFMTI
HHHHHHHHCCCCCCEEEEEEEEHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCEEEEEEC
PEASRLVIQAGALANGGEIFVLDMGEPVKIVNLAKNMIKLSGFSEEDIKIEFSGIRPGEK
CCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHH
LFEELLDADEIHPEQIYPKIYIGKANEINIADLENIIPKILKSNNLKEDLLNFIKK
HHHHHHCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]