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The map label for this gene is gcvH [H]

Identifier: 222150786

GI number: 222150786

Start: 576289

End: 576657

Strand: Direct

Name: gcvH [H]

Synonym: MCCL_0536

Alternate gene names: 222150786

Gene position: 576289-576657 (Clockwise)

Preceding gene: 222150785

Following gene: 222150787

Centisome position: 27.41

GC content: 35.77

Gene sequence:

>369_bases
ATGTCAAACTTAAAATTTTCAAAAGATCATGAGTGGGTTAAATCAGAAGATAACGTAAAAGTAATCGGAATTACAGACTT
TGCACAATCAGAACTAGGGGATATCGTATTCGTTGAACTTCCTGAAGTAGGCGATGAAATTACTACCGGTTCACCATTTG
GTAGCGTTGAGTCGGTTAAAACTGTTTCTGAATTATATGCTCCAATCTCAGGCAAAGTTGTAGAAGTTAACTCTGAATTA
GAAGATTCTCCTGAATTAGTAAACGAATCTGCTATGGATCAAGCATGGATGATCAAAGTTGAAGCAGATGACAGCGAACT
TGATGATTTAATGGATCAAGCGCAATATGATGAAATGATTAATGCATAA

Upstream 100 bases:

>100_bases
GCAGACTATAAACAAACTTGGTTATAAAAAAGATTGAAGAAACGCTTACATTATGTCATTATAAAAGTGTTAAGTATTTT
ATTTATATGGAGGGCTTATA

Downstream 100 bases:

>100_bases
ATGATATACTTTCCTTGATAATAAATTTATCAAGGATTTTTTAATATGACGAAAAAGGTGATGGAAAATGTCTGTAATTC
AGAAGGTCATCGTAGTAGAG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MSNLKFSKDHEWVKSEDNVKVIGITDFAQSELGDIVFVELPEVGDEITTGSPFGSVESVKTVSELYAPISGKVVEVNSEL
EDSPELVNESAMDQAWMIKVEADDSELDDLMDQAQYDEMINA

Sequences:

>Translated_122_residues
MSNLKFSKDHEWVKSEDNVKVIGITDFAQSELGDIVFVELPEVGDEITTGSPFGSVESVKTVSELYAPISGKVVEVNSEL
EDSPELVNESAMDQAWMIKVEADDSELDDLMDQAQYDEMINA
>Mature_121_residues
SNLKFSKDHEWVKSEDNVKVIGITDFAQSELGDIVFVELPEVGDEITTGSPFGSVESVKTVSELYAPISGKVVEVNSELE
DSPELVNESAMDQAWMIKVEADDSELDDLMDQAQYDEMINA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=123, Percent_Identity=44.7154471544715, Blast_Score=123, Evalue=3e-29,
Organism=Homo sapiens, GI49574537, Length=123, Percent_Identity=44.7154471544715, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=47.6190476190476, Blast_Score=117, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=45.2173913043478, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=46.4285714285714, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=39.344262295082, Blast_Score=104, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=42.9752066115703, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13491; Mature: 13360

Theoretical pI: Translated: 3.62; Mature: 3.62

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLKFSKDHEWVKSEDNVKVIGITDFAQSELGDIVFVELPEVGDEITTGSPFGSVESVK
CCCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHH
TVSELYAPISGKVVEVNSELEDSPELVNESAMDQAWMIKVEADDSELDDLMDQAQYDEMI
HHHHHHCCCCCEEEEECCCCCCCHHHHCHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
NA
CC
>Mature Secondary Structure 
SNLKFSKDHEWVKSEDNVKVIGITDFAQSELGDIVFVELPEVGDEITTGSPFGSVESVK
CCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHH
TVSELYAPISGKVVEVNSELEDSPELVNESAMDQAWMIKVEADDSELDDLMDQAQYDEMI
HHHHHHCCCCCEEEEECCCCCCCHHHHCHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
NA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA