Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 222150779

GI number: 222150779

Start: 562870

End: 563331

Strand: Direct

Name: smpB

Synonym: MCCL_0529

Alternate gene names: 222150779

Gene position: 562870-563331 (Clockwise)

Preceding gene: 222150778

Following gene: 222150780

Centisome position: 26.77

GC content: 40.04

Gene sequence:

>462_bases
ATGCCTAAAGGAACAGGAAAAGTATTAGCACAGAACAGAAAAGCAAGTCATGACTATATAATTGAAGAGACGATTGAAGC
GGGGATTGAATTAAAGGGAACAGAAATTAAATCTATCCGACGTGGGAGTGCGAATTTACGCGACTCGTATGCACGTGTGT
ATAAAGGTGAGGTGTTTGTATATAATATGCACATCGCGCCATATGAAGAAGGAAATCGATTCAATCATGACCCGCTCAGA
ACACGTAAATTACTGTTAAAGCGTAAGCAGATTGATAAACTGTTCGGTCAGACACGCGAGCAAGGTTACACGCTAGTACC
GCTTAAACTGTATATTAAAAATGGGTACTGTAAAATGCTGATTGGTGTAGCAAAAGGTAAGAAAGATTACGACAAGCGAC
ATGCACTGAAAGCGAAAGAAGCGAAACGTGAAATGGACCGTGCAATGAAAGAAAGATATTAG

Upstream 100 bases:

>100_bases
CGCGGTGCTGAGAAGAGTAAAAACAAAAATAAAAAACCATTCTATAAATCTAAAGCAGTGAAAAATTCAGGACGCAGAAA
GAAGAAGTAGAGGTGGCGTT

Downstream 100 bases:

>100_bases
AATAAGTGCGACCTGCTTATGTAGCAGGTCGTATTTTGATGTGTGAGATTATGATGAAGTAGCGACTGCTGCTGAAGATG
GCTCATAAATAAAAAACATG

Product: ssrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MPKGTGKVLAQNRKASHDYIIEETIEAGIELKGTEIKSIRRGSANLRDSYARVYKGEVFVYNMHIAPYEEGNRFNHDPLR
TRKLLLKRKQIDKLFGQTREQGYTLVPLKLYIKNGYCKMLIGVAKGKKDYDKRHALKAKEAKREMDRAMKERY

Sequences:

>Translated_153_residues
MPKGTGKVLAQNRKASHDYIIEETIEAGIELKGTEIKSIRRGSANLRDSYARVYKGEVFVYNMHIAPYEEGNRFNHDPLR
TRKLLLKRKQIDKLFGQTREQGYTLVPLKLYIKNGYCKMLIGVAKGKKDYDKRHALKAKEAKREMDRAMKERY
>Mature_152_residues
PKGTGKVLAQNRKASHDYIIEETIEAGIELKGTEIKSIRRGSANLRDSYARVYKGEVFVYNMHIAPYEEGNRFNHDPLRT
RKLLLKRKQIDKLFGQTREQGYTLVPLKLYIKNGYCKMLIGVAKGKKDYDKRHALKAKEAKREMDRAMKERY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=46.5277777777778, Blast_Score=138, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_MACCJ (B9EAH5)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559932.1
- GeneID:   7389627
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0529
- OMA:   EKYEAGI
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17837; Mature: 17706

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKGTGKVLAQNRKASHDYIIEETIEAGIELKGTEIKSIRRGSANLRDSYARVYKGEVFV
CCCCCCCHHCCCCCCCCCHHHHHHHHCCCEECCCHHHHHHCCCCCHHHHHHHHHCCEEEE
YNMHIAPYEEGNRFNHDPLRTRKLLLKRKQIDKLFGQTREQGYTLVPLKLYIKNGYCKML
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCHHHEE
IGVAKGKKDYDKRHALKAKEAKREMDRAMKERY
EEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKGTGKVLAQNRKASHDYIIEETIEAGIELKGTEIKSIRRGSANLRDSYARVYKGEVFV
CCCCCCHHCCCCCCCCCHHHHHHHHCCCEECCCHHHHHHCCCCCHHHHHHHHHCCEEEE
YNMHIAPYEEGNRFNHDPLRTRKLLLKRKQIDKLFGQTREQGYTLVPLKLYIKNGYCKML
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCHHHEE
IGVAKGKKDYDKRHALKAKEAKREMDRAMKERY
EEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA