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The map label for this gene is prfB [H]

Identifier: 222150753

GI number: 222150753

Start: 533514

End: 534566

Strand: Direct

Name: prfB [H]

Synonym: MCCL_0503

Alternate gene names: 222150753

Gene position: 533514-534566 (Clockwise)

Preceding gene: 222150752

Following gene: 222150754

Centisome position: 25.38

GC content: 39.41

Gene sequence:

>1053_bases
TTGACAGTTTTAGGGGGTCTCTTTGACTTAGAAGAAAAAGAGACGAATATTAAGGAATATGAAGAGATGATGGCTGACCC
GGATTTCTGGAATGATGGAGATAAGGCTCAAGATATCATTAATAAGAATAATGCTCTGAAATCAGTTGTTCAGGAGTTTA
GTACACTCGAAGAGGGGCTGGAAAACAGCGAAGTCCTGTTTGAGATGTATAAAGAAGAATCAGATGAAGACACACACCAG
GAACTCATTGAGCAGCTCAATACACTTCAGCAAATTGCAAATGACTTTGAACTTAAGATGTTACTGAGTGGAGAGTTTGA
TGCGAATAATGCGATATTAGAACTGCATCCAGGTGCTGGTGGTACAGAATCACAGGACTGGGGAGAAATGCTGTTAAGAA
TGTATCAACGATTTGCGGATAAAAAGGGTTTCAAAGTTGAAACTGTAGATTATCTGCCGGGAGATGAAGCGGGGATTAAA
AGTGTGACCTTACTGATTAAAGGACACAATGCTTACGGCTATTTAAAGGCGGAAAAAGGTGTTCATCGTCTTGTAAGGAT
TTCTCCATTTGATTCATCCGGACGTCGTCATACTTCATTCGTCTCATGTGAGGTTACACCAGAATTTGACAATGATAATA
TTGAGATTGAAATTAATTCAGAAGACATTAAGATTGATACGTATCGTGCTAGTGGCGCTGGAGGTCAGCACGTTAATACA
ACGGATTCAGCCGTGCGTATTACCCATCAGCCGACAGGTATTGTAGTAACTTGTCAGAATGAACGTTCTCAAATAAAGAA
TAGAGAGCAAGCAATGAAGATGCTTAAAGCGAAGCTATATCAAAAGAAAATAGAAGAACAAGAAGCAGAACTTGCAGCCA
TTCGTGGTGAACAAAAAGAGATTGGCTGGGGAAGCCAGATTCGTTCATATGTATTTCACCCGTATTCTATGGTAAAAGAC
CATAGAACAAACGTTGAAACCGGAAATACAAATGCTGTAATGGATGGCGATATAGATTTATTTATCGATGCATACTTAAA
GAGTCAGCTGTAA

Upstream 100 bases:

>100_bases
TGTGGTAGTGGAAAGAAATATAAAAATTGTCACGGAAGAGGGTAGATTGATTTGGAACAATTTGAAATAAAAAATACACT
TGAAACATTAGCAGAAAAGA

Downstream 100 bases:

>100_bases
CATTATTTTATAAATTTTCCTTTTTTATGAATTATAAGTTATAATTATAGTAATCATATATATTAGGGAGCTGACCAATG
AAAAAATTATATGCAGGTAT

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTVLGGLFDLEEKETNIKEYEEMMADPDFWNDGDKAQDIINKNNALKSVVQEFSTLEEGLENSEVLFEMYKEESDEDTHQ
ELIEQLNTLQQIANDFELKMLLSGEFDANNAILELHPGAGGTESQDWGEMLLRMYQRFADKKGFKVETVDYLPGDEAGIK
SVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSCEVTPEFDNDNIEIEINSEDIKIDTYRASGAGGQHVNT
TDSAVRITHQPTGIVVTCQNERSQIKNREQAMKMLKAKLYQKKIEEQEAELAAIRGEQKEIGWGSQIRSYVFHPYSMVKD
HRTNVETGNTNAVMDGDIDLFIDAYLKSQL

Sequences:

>Translated_350_residues
MTVLGGLFDLEEKETNIKEYEEMMADPDFWNDGDKAQDIINKNNALKSVVQEFSTLEEGLENSEVLFEMYKEESDEDTHQ
ELIEQLNTLQQIANDFELKMLLSGEFDANNAILELHPGAGGTESQDWGEMLLRMYQRFADKKGFKVETVDYLPGDEAGIK
SVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSCEVTPEFDNDNIEIEINSEDIKIDTYRASGAGGQHVNT
TDSAVRITHQPTGIVVTCQNERSQIKNREQAMKMLKAKLYQKKIEEQEAELAAIRGEQKEIGWGSQIRSYVFHPYSMVKD
HRTNVETGNTNAVMDGDIDLFIDAYLKSQL
>Mature_349_residues
TVLGGLFDLEEKETNIKEYEEMMADPDFWNDGDKAQDIINKNNALKSVVQEFSTLEEGLENSEVLFEMYKEESDEDTHQE
LIEQLNTLQQIANDFELKMLLSGEFDANNAILELHPGAGGTESQDWGEMLLRMYQRFADKKGFKVETVDYLPGDEAGIKS
VTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSCEVTPEFDNDNIEIEINSEDIKIDTYRASGAGGQHVNTT
DSAVRITHQPTGIVVTCQNERSQIKNREQAMKMLKAKLYQKKIEEQEAELAAIRGEQKEIGWGSQIRSYVFHPYSMVKDH
RTNVETGNTNAVMDGDIDLFIDAYLKSQL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=293, Percent_Identity=38.5665529010239, Blast_Score=187, Evalue=9e-48,
Organism=Homo sapiens, GI34577120, Length=328, Percent_Identity=35.3658536585366, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=36.4928909952607, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI2367172, Length=348, Percent_Identity=50.5747126436782, Blast_Score=354, Evalue=6e-99,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=40.9836065573771, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=216, Percent_Identity=38.4259259259259, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=282, Percent_Identity=35.8156028368794, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19921226, Length=259, Percent_Identity=39.3822393822394, Blast_Score=168, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39693; Mature: 39562

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLGGLFDLEEKETNIKEYEEMMADPDFWNDGDKAQDIINKNNALKSVVQEFSTLEEGL
CCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCC
ENSEVLFEMYKEESDEDTHQELIEQLNTLQQIANDFELKMLLSGEFDANNAILELHPGAG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCC
GTESQDWGEMLLRMYQRFADKKGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKG
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCCC
VHRLVRISPFDSSGRRHTSFVSCEVTPEFDNDNIEIEINSEDIKIDTYRASGAGGQHVNT
CEEEEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCC
TDSAVRITHQPTGIVVTCQNERSQIKNREQAMKMLKAKLYQKKIEEQEAELAAIRGEQKE
CCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IGWGSQIRSYVFHPYSMVKDHRTNVETGNTNAVMDGDIDLFIDAYLKSQL
CCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TVLGGLFDLEEKETNIKEYEEMMADPDFWNDGDKAQDIINKNNALKSVVQEFSTLEEGL
CCCCCCCCCCHHHCCHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCC
ENSEVLFEMYKEESDEDTHQELIEQLNTLQQIANDFELKMLLSGEFDANNAILELHPGAG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCC
GTESQDWGEMLLRMYQRFADKKGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKG
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCCC
VHRLVRISPFDSSGRRHTSFVSCEVTPEFDNDNIEIEINSEDIKIDTYRASGAGGQHVNT
CEEEEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCC
TDSAVRITHQPTGIVVTCQNERSQIKNREQAMKMLKAKLYQKKIEEQEAELAAIRGEQKE
CCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IGWGSQIRSYVFHPYSMVKDHRTNVETGNTNAVMDGDIDLFIDAYLKSQL
CCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]