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The map label for this gene is nrdF [H]

Identifier: 222150734

GI number: 222150734

Start: 514388

End: 515356

Strand: Direct

Name: nrdF [H]

Synonym: MCCL_0484

Alternate gene names: 222150734

Gene position: 514388-515356 (Clockwise)

Preceding gene: 222150733

Following gene: 222150735

Centisome position: 24.47

GC content: 37.25

Gene sequence:

>969_bases
ATGATAGCTGTAAACTGGAATACACAAGAAGATATGACGAACATGTTCTGGAGACAGAATATCTCACAGATGTGGGTAGA
ATCTGAATTTAAAGTATCAAAAGACATCGCAAGCTGGAAAGAGTTAACTGATGATGAGAAGTTTACATTTAATCATGCGC
TTGCTGGACTTACTGGATTAGATACGCATCAGGCAGACGATGGTATGCCGCTTATTGGTTTACATACGCAAGACCTGAGA
AAGAAAGCAGTATATTCGTTTATGGGGATGATGGAGCAAATCCATGCGAAAAGTTATTCACATATCTTTACAACGTTATT
ACCATCTGCTGAAACGAACGATTTACTGGATAACTGGGTTGTGAATGAACCACATCTAAAGTTTAAATCAGAAAAGATCG
TTTCTAGATACCATAAACTTTGGGGCAAAGAAGCAACAATCTATGATCAGTATATGGCACGTGTTGCGAGCGTATTCTTA
GAGACATTCTTATTCTATAGTGGCTTTTATTATCCACTATACCTAGCTGGTCAAGGTCGCATGACGACGAGTGGAGAGAT
TATTCGTAAGATTCTGCTTGATGAATCAATTCACGGTGTGTTTACTGGATTGGATGCACAAAGCTTACGCAATGAAATGA
CAGAAGATGAGAAAATTCGTGCAGATAAAGAAATGTACGACCTACTGAAAGAGTTATATGCAAATGAAGAAAGTTATACA
AAGATGTTATATGATCGCGTAGGTTTAACTGAAGATGTGCTCAACTACGTTCAGTATAACGGTAATAAAGCATTAGCCAA
TTTAGGGTTTGAACCATACTTCGAAGAAAAAGGATTCAATCCCATTATCGAAAATGCTTTAAACACTTCTACGAAAAACC
ATGACTTCTTCAGCGTTAAAGGTGACGGTTATGTTATCGCATTAAACGTAGAAGCATTAACAGACGAAGACTTCCAATTT
GAATCTTAA

Upstream 100 bases:

>100_bases
ATTAAGTGTAGAAGAATGTACAAGCTGTGCAATTTAAACAATATTAAAGGCTGAGCAATCAGCCTTTAAAATATGTATAA
CAATAAAAAGGACTGAAATT

Downstream 100 bases:

>100_bases
TATTTGACGAGCTTGTGACAAATGTCGCAAGCTCTTTTTGAGTACAGTATTTGTCACAAATTGGACATTGCTAGAAATGT
ATAAAACAGACATAATTGTA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MIAVNWNTQEDMTNMFWRQNISQMWVESEFKVSKDIASWKELTDDEKFTFNHALAGLTGLDTHQADDGMPLIGLHTQDLR
KKAVYSFMGMMEQIHAKSYSHIFTTLLPSAETNDLLDNWVVNEPHLKFKSEKIVSRYHKLWGKEATIYDQYMARVASVFL
ETFLFYSGFYYPLYLAGQGRMTTSGEIIRKILLDESIHGVFTGLDAQSLRNEMTEDEKIRADKEMYDLLKELYANEESYT
KMLYDRVGLTEDVLNYVQYNGNKALANLGFEPYFEEKGFNPIIENALNTSTKNHDFFSVKGDGYVIALNVEALTDEDFQF
ES

Sequences:

>Translated_322_residues
MIAVNWNTQEDMTNMFWRQNISQMWVESEFKVSKDIASWKELTDDEKFTFNHALAGLTGLDTHQADDGMPLIGLHTQDLR
KKAVYSFMGMMEQIHAKSYSHIFTTLLPSAETNDLLDNWVVNEPHLKFKSEKIVSRYHKLWGKEATIYDQYMARVASVFL
ETFLFYSGFYYPLYLAGQGRMTTSGEIIRKILLDESIHGVFTGLDAQSLRNEMTEDEKIRADKEMYDLLKELYANEESYT
KMLYDRVGLTEDVLNYVQYNGNKALANLGFEPYFEEKGFNPIIENALNTSTKNHDFFSVKGDGYVIALNVEALTDEDFQF
ES
>Mature_322_residues
MIAVNWNTQEDMTNMFWRQNISQMWVESEFKVSKDIASWKELTDDEKFTFNHALAGLTGLDTHQADDGMPLIGLHTQDLR
KKAVYSFMGMMEQIHAKSYSHIFTTLLPSAETNDLLDNWVVNEPHLKFKSEKIVSRYHKLWGKEATIYDQYMARVASVFL
ETFLFYSGFYYPLYLAGQGRMTTSGEIIRKILLDESIHGVFTGLDAQSLRNEMTEDEKIRADKEMYDLLKELYANEESYT
KMLYDRVGLTEDVLNYVQYNGNKALANLGFEPYFEEKGFNPIIENALNTSTKNHDFFSVKGDGYVIALNVEALTDEDFQF
ES

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=274, Percent_Identity=24.0875912408759, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI289177074, Length=274, Percent_Identity=24.0875912408759, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789031, Length=322, Percent_Identity=36.9565217391304, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788567, Length=302, Percent_Identity=26.158940397351, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 37294; Mature: 37294

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVNWNTQEDMTNMFWRQNISQMWVESEFKVSKDIASWKELTDDEKFTFNHALAGLTGL
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCC
DTHQADDGMPLIGLHTQDLRKKAVYSFMGMMEQIHAKSYSHIFTTLLPSAETNDLLDNWV
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCC
VNEPHLKFKSEKIVSRYHKLWGKEATIYDQYMARVASVFLETFLFYSGFYYPLYLAGQGR
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
MTTSGEIIRKILLDESIHGVFTGLDAQSLRNEMTEDEKIRADKEMYDLLKELYANEESYT
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHH
KMLYDRVGLTEDVLNYVQYNGNKALANLGFEPYFEEKGFNPIIENALNTSTKNHDFFSVK
HHHHHHCCCHHHHHHHHHHCCCCCEECCCCCCCHHCCCCCHHHHHHHCCCCCCCCEEEEC
GDGYVIALNVEALTDEDFQFES
CCCEEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MIAVNWNTQEDMTNMFWRQNISQMWVESEFKVSKDIASWKELTDDEKFTFNHALAGLTGL
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCC
DTHQADDGMPLIGLHTQDLRKKAVYSFMGMMEQIHAKSYSHIFTTLLPSAETNDLLDNWV
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCC
VNEPHLKFKSEKIVSRYHKLWGKEATIYDQYMARVASVFLETFLFYSGFYYPLYLAGQGR
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
MTTSGEIIRKILLDESIHGVFTGLDAQSLRNEMTEDEKIRADKEMYDLLKELYANEESYT
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHH
KMLYDRVGLTEDVLNYVQYNGNKALANLGFEPYFEEKGFNPIIENALNTSTKNHDFFSVK
HHHHHHCCCHHHHHHHHHHCCCCCEECCCCCCCHHCCCCCHHHHHHHCCCCCCCCEEEEC
GDGYVIALNVEALTDEDFQFES
CCCEEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]