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The map label for this gene is nrdE [H]

Identifier: 222150733

GI number: 222150733

Start: 512219

End: 514324

Strand: Direct

Name: nrdE [H]

Synonym: MCCL_0483

Alternate gene names: 222150733

Gene position: 512219-514324 (Clockwise)

Preceding gene: 222150732

Following gene: 222150734

Centisome position: 24.36

GC content: 36.23

Gene sequence:

>2106_bases
GTGAATATCATACAAGAGAAACAGTACAATCATATTGAACTTAATAACCAGGTTACGAAACGTCGAGAAGATGGCTTCTT
TGATATTGATAAAGATCAAGAAGCACTTAAAGTTTACTTAGAAGAAATAAAAGATAAAACGATAACTTTTGACACACCGA
TTGCACGTCTTAAGTACCTCGTTGAACAAGATTTTTATTATGATTTATTTAATGAGTATAACGAAACAGATTTGAATGAT
ATTATCAAGTATGCACAAGACATTGAATTCAACTTTGCCAGCTATATGTCAGCAAGTAAATTCTTTAAAGATTACGCATT
GAAAACAAATGATAAACAGCAATATCTAGAAGATTATAAAGAGCATGTTATCATCGTAAGCCTATATTTAGCTAAAGGTG
ATGTAAATAAGGCGAAACAATTAATCTCTGCGATGATTGAGCAACGTTATCAACCAGCGACACCTACATTTTTAAATGCT
GGTCGTGCAAGACGCGGTGAGCTTGTATCTTGTTTCTTGCTAGAAGTAGATGATAGTTTAAATTCTATCAACTATATCGA
CTCAACTGCGAAACAATTATCTAAAATTGGTGGCGGTGTTGCAATCAACTTGTCAAAGTTACGTGCAAGAGGAGAAGCGA
TCAAAGGCATTAAAGGTGTAGCTAAAGGTGTATTACCTGTAGCTAAGGCGCTCGAAGGTGGATTTAGTTATGCAGACCAG
CTAGGACAACGTCCAGGTGCAGGCGCTGTATACTTAAACATCTTCCATTATGATGTGCTAGAGTTCTTAGATACGAAAAA
AGTAAATGCTGATGAGGACTTACGTTTATCGACGATTTCTACTGGACTTATCGTACCAAGTAAATTCTTTGATTTGGCGA
AAGAGGGCAAAGATTTCTTTATGTTTGCACCACATACAGTTGAACGTGAATATGGTTTAACGCTAGACGATCTGAACATT
GATGAAATGTATGACGAACTTGTGGCAAATCCAAATATTATGAAGAAATCTAAAGATGCGCGTGAAATGTTAAACTTGAT
TGCTCAAACGCAACTTCAGTCGGGTTATCCATACTTAATGTTTAAGGATAATGCGAACAAAGTGCATGCAAATTCGAATA
TCGGTCAGATTAAGATGAGCAATTTATGTACAGAGATCTTCCAGTTACAAGAAACATCCATTATTAATGATTATGGCACT
GAAGATGAAATCAAACGCGATATATCATGTAACTTAGGATCATTGAATATTGTGAATGTAATGGAATCTAAGAAATTTAA
AGATTCAGTACATACTGGTATGGATGCACTTACAACTGTAAGCGATGAAGCGGATATTAAAAATGCACCAGGTGTCGCGA
AAGCGAACCGTGAGTTACATTCGGTTGGTTTAGGGGTCATGAACCTGCATGGCTATTTAGCTAAAAACCAAATCGGTTAT
GAATCAGAGGAAGCGAAAGAATTTGCTGCGACGTTCTTTATGATGCTGAACTTCTATTCTTTACAACGTTCTATGGAAAT
CGCAATGGAACGTAACGAAACGTTTGCTGACTTTGAAAAATCAGATTATGCATCTGGTAAGTACTTTGATAAATACGTTG
CAACAGATTTTCGCCCGAAATCTGACAAAGTGACTGCATTATTTGAGGGTATCGACATGCCATCTCCGGAAGATTGGGCA
GACTTAAAGGATAAAGTAATGAAGAATGGTTTATTCCATGCGTATCGTTTAGCGATTGCGCCGACACAAAGCATTTCTTA
TGTACAAAATGCAACGAGTTCTGTAATGCCGATTGTTGACCAGATTGAGCGTAGAACGTATGGTAATGCCGAAACATTCT
ATCCTATGCCATTTTTATCACCTGAGACGATGTGGTTCTACAAATCAGCATTCAATACGGATCAAATGAAGTTGATTGAT
TTAATTGCAACGATTCAGGAACATATAGATCAGGGGATTAGTACAATCTTATTTGTAAATAGTGACATATCTACGAGAGA
GCTTGCAAGACTTTATGTTTATGCGCATCATAAAGGATTAAAGTCGCTTTACTACACAAGAAATAAATTATTAAGTGTAG
AAGAATGTACAAGCTGTGCAATTTAA

Upstream 100 bases:

>100_bases
TTGATAAGTAGTACGTATCATGTACCATTATTAATGAAGTTTGAACTACACGGTAATGATAATGATGTGAAAGAATTTAA
TATAAAGGTGGAAGAAATCA

Downstream 100 bases:

>100_bases
ACAATATTAAAGGCTGAGCAATCAGCCTTTAAAATATGTATAACAATAAAAAGGACTGAAATTATGATAGCTGTAAACTG
GAATACACAAGAAGATATGA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MNIIQEKQYNHIELNNQVTKRREDGFFDIDKDQEALKVYLEEIKDKTITFDTPIARLKYLVEQDFYYDLFNEYNETDLND
IIKYAQDIEFNFASYMSASKFFKDYALKTNDKQQYLEDYKEHVIIVSLYLAKGDVNKAKQLISAMIEQRYQPATPTFLNA
GRARRGELVSCFLLEVDDSLNSINYIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPVAKALEGGFSYADQ
LGQRPGAGAVYLNIFHYDVLEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFFMFAPHTVEREYGLTLDDLNI
DEMYDELVANPNIMKKSKDAREMLNLIAQTQLQSGYPYLMFKDNANKVHANSNIGQIKMSNLCTEIFQLQETSIINDYGT
EDEIKRDISCNLGSLNIVNVMESKKFKDSVHTGMDALTTVSDEADIKNAPGVAKANRELHSVGLGVMNLHGYLAKNQIGY
ESEEAKEFAATFFMMLNFYSLQRSMEIAMERNETFADFEKSDYASGKYFDKYVATDFRPKSDKVTALFEGIDMPSPEDWA
DLKDKVMKNGLFHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPETMWFYKSAFNTDQMKLID
LIATIQEHIDQGISTILFVNSDISTRELARLYVYAHHKGLKSLYYTRNKLLSVEECTSCAI

Sequences:

>Translated_701_residues
MNIIQEKQYNHIELNNQVTKRREDGFFDIDKDQEALKVYLEEIKDKTITFDTPIARLKYLVEQDFYYDLFNEYNETDLND
IIKYAQDIEFNFASYMSASKFFKDYALKTNDKQQYLEDYKEHVIIVSLYLAKGDVNKAKQLISAMIEQRYQPATPTFLNA
GRARRGELVSCFLLEVDDSLNSINYIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPVAKALEGGFSYADQ
LGQRPGAGAVYLNIFHYDVLEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFFMFAPHTVEREYGLTLDDLNI
DEMYDELVANPNIMKKSKDAREMLNLIAQTQLQSGYPYLMFKDNANKVHANSNIGQIKMSNLCTEIFQLQETSIINDYGT
EDEIKRDISCNLGSLNIVNVMESKKFKDSVHTGMDALTTVSDEADIKNAPGVAKANRELHSVGLGVMNLHGYLAKNQIGY
ESEEAKEFAATFFMMLNFYSLQRSMEIAMERNETFADFEKSDYASGKYFDKYVATDFRPKSDKVTALFEGIDMPSPEDWA
DLKDKVMKNGLFHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPETMWFYKSAFNTDQMKLID
LIATIQEHIDQGISTILFVNSDISTRELARLYVYAHHKGLKSLYYTRNKLLSVEECTSCAI
>Mature_701_residues
MNIIQEKQYNHIELNNQVTKRREDGFFDIDKDQEALKVYLEEIKDKTITFDTPIARLKYLVEQDFYYDLFNEYNETDLND
IIKYAQDIEFNFASYMSASKFFKDYALKTNDKQQYLEDYKEHVIIVSLYLAKGDVNKAKQLISAMIEQRYQPATPTFLNA
GRARRGELVSCFLLEVDDSLNSINYIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPVAKALEGGFSYADQ
LGQRPGAGAVYLNIFHYDVLEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFFMFAPHTVEREYGLTLDDLNI
DEMYDELVANPNIMKKSKDAREMLNLIAQTQLQSGYPYLMFKDNANKVHANSNIGQIKMSNLCTEIFQLQETSIINDYGT
EDEIKRDISCNLGSLNIVNVMESKKFKDSVHTGMDALTTVSDEADIKNAPGVAKANRELHSVGLGVMNLHGYLAKNQIGY
ESEEAKEFAATFFMMLNFYSLQRSMEIAMERNETFADFEKSDYASGKYFDKYVATDFRPKSDKVTALFEGIDMPSPEDWA
DLKDKVMKNGLFHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPETMWFYKSAFNTDQMKLID
LIATIQEHIDQGISTILFVNSDISTRELARLYVYAHHKGLKSLYYTRNKLLSVEECTSCAI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=661, Percent_Identity=26.3237518910741, Blast_Score=198, Evalue=2e-50,
Organism=Escherichia coli, GI1789030, Length=687, Percent_Identity=43.5225618631732, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI2367133, Length=555, Percent_Identity=25.045045045045, Blast_Score=141, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17555242, Length=663, Percent_Identity=26.5460030165913, Blast_Score=195, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6322123, Length=677, Percent_Identity=25.1107828655835, Blast_Score=190, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6320914, Length=678, Percent_Identity=24.6312684365782, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17352481, Length=669, Percent_Identity=26.0089686098655, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 79766; Mature: 79766

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIQEKQYNHIELNNQVTKRREDGFFDIDKDQEALKVYLEEIKDKTITFDTPIARLKYL
CCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHH
VEQDFYYDLFNEYNETDLNDIIKYAQDIEFNFASYMSASKFFKDYALKTNDKQQYLEDYK
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EHVIIVSLYLAKGDVNKAKQLISAMIEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSL
CCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEEEEEEECCCC
NSINYIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPVAKALEGGFSYADQ
CHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LGQRPGAGAVYLNIFHYDVLEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFF
HCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEE
MFAPHTVEREYGLTLDDLNIDEMYDELVANPNIMKKSKDAREMLNLIAQTQLQSGYPYLM
EECCCEECHHCCCEECCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCEEE
FKDNANKVHANSNIGQIKMSNLCTEIFQLQETSIINDYGTEDEIKRDISCNLGSLNIVNV
EECCCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEHHH
MESKKFKDSVHTGMDALTTVSDEADIKNAPGVAKANRELHSVGLGVMNLHGYLAKNQIGY
HHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
ESEEAKEFAATFFMMLNFYSLQRSMEIAMERNETFADFEKSDYASGKYFDKYVATDFRPK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHCCCCCC
SDKVTALFEGIDMPSPEDWADLKDKVMKNGLFHAYRLAIAPTQSISYVQNATSSVMPIVD
CCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHH
QIERRTYGNAETFYPMPFLSPETMWFYKSAFNTDQMKLIDLIATIQEHIDQGISTILFVN
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
SDISTRELARLYVYAHHKGLKSLYYTRNKLLSVEECTSCAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MNIIQEKQYNHIELNNQVTKRREDGFFDIDKDQEALKVYLEEIKDKTITFDTPIARLKYL
CCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHH
VEQDFYYDLFNEYNETDLNDIIKYAQDIEFNFASYMSASKFFKDYALKTNDKQQYLEDYK
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EHVIIVSLYLAKGDVNKAKQLISAMIEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSL
CCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEEEEEEECCCC
NSINYIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPVAKALEGGFSYADQ
CHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LGQRPGAGAVYLNIFHYDVLEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFF
HCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEE
MFAPHTVEREYGLTLDDLNIDEMYDELVANPNIMKKSKDAREMLNLIAQTQLQSGYPYLM
EECCCEECHHCCCEECCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCEEE
FKDNANKVHANSNIGQIKMSNLCTEIFQLQETSIINDYGTEDEIKRDISCNLGSLNIVNV
EECCCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEHHH
MESKKFKDSVHTGMDALTTVSDEADIKNAPGVAKANRELHSVGLGVMNLHGYLAKNQIGY
HHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
ESEEAKEFAATFFMMLNFYSLQRSMEIAMERNETFADFEKSDYASGKYFDKYVATDFRPK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHCCCCCC
SDKVTALFEGIDMPSPEDWADLKDKVMKNGLFHAYRLAIAPTQSISYVQNATSSVMPIVD
CCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHH
QIERRTYGNAETFYPMPFLSPETMWFYKSAFNTDQMKLIDLIATIQEHIDQGISTILFVN
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
SDISTRELARLYVYAHHKGLKSLYYTRNKLLSVEECTSCAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]