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The map label for this gene is thiC [H]

Identifier: 222150548

GI number: 222150548

Start: 304994

End: 306709

Strand: Direct

Name: thiC [H]

Synonym: MCCL_0298

Alternate gene names: 222150548

Gene position: 304994-306709 (Clockwise)

Preceding gene: 222150547

Following gene: 222150550

Centisome position: 14.51

GC content: 42.66

Gene sequence:

>1716_bases
ATGGTAAATTTGAAGAAGTCTTTTCCGGCAAGTAAGAGGATCTTTAAAGAGGGTAAGGGCGAGGATTCGGATATTAAAGT
ACCTTTCCGTGAAATTACGTTAACGGATACAGTGACAGATCAAGGTACTGCTTCCAATGCACCAGTTGTTGTCTATGACA
CAGGTGGCATATATCACGATGATACATATGATATTGATGTTGAGCGTGGTATCCCTAAACTACGCGAGCGATGGATTGAT
GATCGCAATGACATAGAGCGCTACACAGGACGCAGAGTACTGGCGGTGGATAATGGTTTTAAAGATATCACGCATAACAA
GTTTGTGGAGACGCCATTTAAGTATGAACCCAAACGTGCGCTTGAAGGTAAGAACGTGACACAGATGCATTATGCAAAGC
AAGGAATCATTACGAAGGAGATGAAGTTCGTCGCGATTCGTGAGAACGTCGATCCTGAATTTGTACGCAGTGAAATTGCA
AGAGGGCGAGCAATTATTCCAAACAATATTAATCACCCAGAATCTGAACCGATGATTATTGGAAAGAACTTTAAGGTGAA
GGTTAATGCGAATATCGGAAACTCTGCGGTATCCTCATCAATCGAAGATGAGATTGAAAAGATGGTGTGGGCGACACATT
GGGGAGCAGATACGATTATGGATTTATCTACAGGTAAGAATATTCATGCGACACGTGAATATTTACTGAGAAATTCACCG
GTACCTGTTGGAACGGTGCCTATTTATCAAGCGTTAGAAAAAGTAGATGGCATTGCTGAAGACTTAACGTGGGAAGTGTA
TCGAGATACGTTAATTGAACAAGCAGAACAAGGCGTGGACTATTTTACAATTCATGCAGGTGTATTATTGAGGTATGTGC
CGATGACAGTGAATCGTCTGACAGGCATTGTTTCACGTGGAGGTTCAATTATGGCGCAGTGGTGTTTAGCCCATCATGAA
GAAAGCTTCTTATACACGCACTTCGAAGAAATTTGTGAAATCTTAAAGACTTATGATATTGCAGTATCACTCGGTGATGG
ATTAAGACCAGGGTCAATTTATGATGCGAACGATGAGAGTCAGTTGGCAGAACTGAAGACGCTTGGTGAACTGACGAAGA
TTGCTTGGGCACATGACGTACAAGTGATGATAGAAGGACCGGGACATGTACCGCTTCATAAGATCAAAGAGAATCAGGAC
CTTGCAGATTTCTATTGTAATGAAGCACCATTCTATACGTTAGGACCATTGACGACAGATATTGCACCTGCATATGACCA
TATTACATCAGCGATTGGCGCAGCAAACATCGGTTCATATGGTACAGCGATGTTATGCTATGTTACACCGAAGGAACATC
TCGGGCTACCGAATAAAGATGATGTACGCACGGGCGTTGTAACATATAAGATAGCAGCACATGCAGCGGATCTTGCGAAA
GGCTTACCGGGAGCCGATGAACGAGACAATGCCATCAGTAAGGCACGCTTTGAATTCCGCTGGATTGATCAGTTTAATTT
AGCTCTGGATCCAGAGCGTGCGAAGGAATATCATGATGAAACCTTACCTGCCGAAGCAGCGAAAGTAGCACATTTCTGTA
GTATGTGCGGGCCTAAGTTCTGTTCAATGCGTATTTCCCATGATTTGCGTAAAGATAAAGGAATGCAGGAAATGGCTGAT
AAATTCAAAGCACAAGGTAGTGAAATTTACTCTTAG

Upstream 100 bases:

>100_bases
TGGTTAGTACCAGCGTAGGGAAGCGAGCGTGTATACTTATGATGTTTTTTGTCGTGAATTTTCACCTCCTAGTAATGAAT
ATAACTAGGAGGTTTTTTGT

Downstream 100 bases:

>100_bases
AATTTGCTGTGAATCGGAGCGCAGTTTATTCATATATAAAAAGCACCACCCTTATACCAGACCGGGGGTGGTGCTTTATT
TTATATAGTCACTGAAATTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MVNLKKSFPASKRIFKEGKGEDSDIKVPFREITLTDTVTDQGTASNAPVVVYDTGGIYHDDTYDIDVERGIPKLRERWID
DRNDIERYTGRRVLAVDNGFKDITHNKFVETPFKYEPKRALEGKNVTQMHYAKQGIITKEMKFVAIRENVDPEFVRSEIA
RGRAIIPNNINHPESEPMIIGKNFKVKVNANIGNSAVSSSIEDEIEKMVWATHWGADTIMDLSTGKNIHATREYLLRNSP
VPVGTVPIYQALEKVDGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYVPMTVNRLTGIVSRGGSIMAQWCLAHHE
ESFLYTHFEEICEILKTYDIAVSLGDGLRPGSIYDANDESQLAELKTLGELTKIAWAHDVQVMIEGPGHVPLHKIKENQD
LADFYCNEAPFYTLGPLTTDIAPAYDHITSAIGAANIGSYGTAMLCYVTPKEHLGLPNKDDVRTGVVTYKIAAHAADLAK
GLPGADERDNAISKARFEFRWIDQFNLALDPERAKEYHDETLPAEAAKVAHFCSMCGPKFCSMRISHDLRKDKGMQEMAD
KFKAQGSEIYS

Sequences:

>Translated_571_residues
MVNLKKSFPASKRIFKEGKGEDSDIKVPFREITLTDTVTDQGTASNAPVVVYDTGGIYHDDTYDIDVERGIPKLRERWID
DRNDIERYTGRRVLAVDNGFKDITHNKFVETPFKYEPKRALEGKNVTQMHYAKQGIITKEMKFVAIRENVDPEFVRSEIA
RGRAIIPNNINHPESEPMIIGKNFKVKVNANIGNSAVSSSIEDEIEKMVWATHWGADTIMDLSTGKNIHATREYLLRNSP
VPVGTVPIYQALEKVDGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYVPMTVNRLTGIVSRGGSIMAQWCLAHHE
ESFLYTHFEEICEILKTYDIAVSLGDGLRPGSIYDANDESQLAELKTLGELTKIAWAHDVQVMIEGPGHVPLHKIKENQD
LADFYCNEAPFYTLGPLTTDIAPAYDHITSAIGAANIGSYGTAMLCYVTPKEHLGLPNKDDVRTGVVTYKIAAHAADLAK
GLPGADERDNAISKARFEFRWIDQFNLALDPERAKEYHDETLPAEAAKVAHFCSMCGPKFCSMRISHDLRKDKGMQEMAD
KFKAQGSEIYS
>Mature_571_residues
MVNLKKSFPASKRIFKEGKGEDSDIKVPFREITLTDTVTDQGTASNAPVVVYDTGGIYHDDTYDIDVERGIPKLRERWID
DRNDIERYTGRRVLAVDNGFKDITHNKFVETPFKYEPKRALEGKNVTQMHYAKQGIITKEMKFVAIRENVDPEFVRSEIA
RGRAIIPNNINHPESEPMIIGKNFKVKVNANIGNSAVSSSIEDEIEKMVWATHWGADTIMDLSTGKNIHATREYLLRNSP
VPVGTVPIYQALEKVDGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYVPMTVNRLTGIVSRGGSIMAQWCLAHHE
ESFLYTHFEEICEILKTYDIAVSLGDGLRPGSIYDANDESQLAELKTLGELTKIAWAHDVQVMIEGPGHVPLHKIKENQD
LADFYCNEAPFYTLGPLTTDIAPAYDHITSAIGAANIGSYGTAMLCYVTPKEHLGLPNKDDVRTGVVTYKIAAHAADLAK
GLPGADERDNAISKARFEFRWIDQFNLALDPERAKEYHDETLPAEAAKVAHFCSMCGPKFCSMRISHDLRKDKGMQEMAD
KFKAQGSEIYS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=608, Percent_Identity=63.3223684210526, Blast_Score=784, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 64052; Mature: 64052

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLKKSFPASKRIFKEGKGEDSDIKVPFREITLTDTVTDQGTASNAPVVVYDTGGIYHD
CCCCCCCCCHHHHHHHCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCEEEEECCCEECC
DTYDIDVERGIPKLRERWIDDRNDIERYTGRRVLAVDNGFKDITHNKFVETPFKYEPKRA
CCEEEEHHCCCHHHHHHHCCCHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCCCCCHHH
LEGKNVTQMHYAKQGIITKEMKFVAIRENVDPEFVRSEIARGRAIIPNNINHPESEPMII
CCCCCCHHHHHHHHCCEECCEEEEEEECCCCHHHHHHHHHCCCEECCCCCCCCCCCCEEE
GKNFKVKVNANIGNSAVSSSIEDEIEKMVWATHWGADTIMDLSTGKNIHATREYLLRNSP
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
VPVGTVPIYQALEKVDGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYVPMTVNRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
TGIVSRGGSIMAQWCLAHHEESFLYTHFEEICEILKTYDIAVSLGDGLRPGSIYDANDES
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEECCCCHH
QLAELKTLGELTKIAWAHDVQVMIEGPGHVPLHKIKENQDLADFYCNEAPFYTLGPLTTD
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCHHHHHCCCCCCEEECCCCCC
IAPAYDHITSAIGAANIGSYGTAMLCYVTPKEHLGLPNKDDVRTGVVTYKIAAHAADLAK
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHH
GLPGADERDNAISKARFEFRWIDQFNLALDPERAKEYHDETLPAEAAKVAHFCSMCGPKF
CCCCCCCCCCHHHHHEEEEEEEECCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHCCHHH
CSMRISHDLRKDKGMQEMADKFKAQGSEIYS
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVNLKKSFPASKRIFKEGKGEDSDIKVPFREITLTDTVTDQGTASNAPVVVYDTGGIYHD
CCCCCCCCCHHHHHHHCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCEEEEECCCEECC
DTYDIDVERGIPKLRERWIDDRNDIERYTGRRVLAVDNGFKDITHNKFVETPFKYEPKRA
CCEEEEHHCCCHHHHHHHCCCHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCCCCCHHH
LEGKNVTQMHYAKQGIITKEMKFVAIRENVDPEFVRSEIARGRAIIPNNINHPESEPMII
CCCCCCHHHHHHHHCCEECCEEEEEEECCCCHHHHHHHHHCCCEECCCCCCCCCCCCEEE
GKNFKVKVNANIGNSAVSSSIEDEIEKMVWATHWGADTIMDLSTGKNIHATREYLLRNSP
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
VPVGTVPIYQALEKVDGIAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYVPMTVNRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
TGIVSRGGSIMAQWCLAHHEESFLYTHFEEICEILKTYDIAVSLGDGLRPGSIYDANDES
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEECCCCHH
QLAELKTLGELTKIAWAHDVQVMIEGPGHVPLHKIKENQDLADFYCNEAPFYTLGPLTTD
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCHHHHHCCCCCCEEECCCCCC
IAPAYDHITSAIGAANIGSYGTAMLCYVTPKEHLGLPNKDDVRTGVVTYKIAAHAADLAK
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHH
GLPGADERDNAISKARFEFRWIDQFNLALDPERAKEYHDETLPAEAAKVAHFCSMCGPKF
CCCCCCCCCCHHHHHEEEEEEEECCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHCCHHH
CSMRISHDLRKDKGMQEMADKFKAQGSEIYS
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA