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The map label for this gene is lutB

Identifier: 222150423

GI number: 222150423

Start: 178777

End: 180210

Strand: Direct

Name: lutB

Synonym: MCCL_0173

Alternate gene names: 222150423

Gene position: 178777-180210 (Clockwise)

Preceding gene: 222150422

Following gene: 222150424

Centisome position: 8.5

GC content: 39.68

Gene sequence:

>1434_bases
ATGAGTATGAGAATAGGTAACGAAACGTTTAAGCAGGCAGTTAAGACGGAACTTAAAGATGACTTCATGCGTGGTGCTGT
GAGTAGTGCACAAGATAGATTAAGAGAACGCAAACTTAAAACTCAAGAAGATCTTGGAAACTGGGAAGAGTGGAGAGACC
ATTCTGAAGAGATTCGTCAGCATACGCTTGCGAACCTTGACTATTACTTAAACATGCTTTCTGAGAATGTCGCTGCAAAA
GGTGGACATGTATTCTTCGCTGAAACAGCAGAAGAGGCCAATCAGTATGTGCAAAAAGTACTACGTGACAAAAATGCTAA
AAAAGTAGCAAAATCAAAATCAATGGTAACTGAAGAAATTGGTCTGAACGAAGCGATGGAAGCGGTAGGATGTGAAGTCA
TTGAGACAGACTTAGGAGAATATATTCTTCAAGTCGATGATCATAATCCACCTTCACATATCGTTGTACCTGCACTTCAT
CTTAATAAAGAGCAAATCGAAAAGGTCTTCAAAGAAAAACTTGGATATGATAAATCGAGTCAACCGGAAGAACTTGCGTT
ATTTGCGCGTCAAAAGCTACGTGAAGAGTTTCTTTCTGCAGATGTTGGGGTTACCGGGTGTAACTTTGGTGTTGCAGAAA
GTGGTTCATTCAGCTTAGTTACAAATGAAGGTAACGCACGTATGGTAACAACATTACCTAAAACTCAGATTACAGTTATG
GGTATGGAACGTTTAGTTCCTACGCATGAAGAACTAGAAGTATTAGTGTCAATGCTTACGCGTTCGGCTGTAGGGCAAAA
ACTAACAAGTTATGTGACAACTTTAACAGGACCACGTGCGGAAGATGAGATTGATGGGCCAGAAGAATTCCATTTAATTA
TTGTGGATAATGGACGCTCTAAAATTTTAGGAACGCAATTCCAGTCTATTCTACAATGTATTCGTTGTGCTGCTTGTGTC
AACGTTTGTCCTGTTTATCGTCAAACAGGTGGCCATTCATATGGTTCGATTTATCCAGGACCAATTGGTGCGGTATTATC
ACCATTACTTGGTGGATATGATACTTACAAAGAATTGCCATATGCTTCTACATTATGTGGTGCTTGTACTGAAGCATGTC
CGGTTAAGATCCCGTTACATGACTTATTACTTGAACATAGACGTGTTATTGTGGAGGAAGAACATATGTCACCTGTTGCA
GAACGTCTTGTCATGAAAGGTTTTGCACAAGGAGCTAGTCATTCAACTTTATTTAAATACGGTACTAAAGCAGCACCAGC
ATTAATGTCACCGTTTGAAAACAAAGATAAAGGTATGATTACTAAAGGTCCTAAGCCTTTACAAGCATGGACAAATTCTC
GCGATTTCCCAATGCCGGATGATGAAAACTTCCGCGACTGGATGCAAAATAGATTGAAAAGAGGTGCGAAATAA

Upstream 100 bases:

>100_bases
TGATGAATATTGATGGTAGATTAAGACGTCGTGGTGTTGATGTGAAAGCTGTACATATCATTGAAATCTTAAATAACCAA
GTTGAGGCGGTGACTACACG

Downstream 100 bases:

>100_bases
TGGAAAGAGGCACAATTCACAATAAAGGGTCATTCTTAAGTAACATCTCTAAAAATTTACAGCGTGAAATGCCACAGCAT
GTAGAACGTCCTGAATGGCA

Product: iron-sulfur cluster binding protein YvfW

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSMRIGNETFKQAVKTELKDDFMRGAVSSAQDRLRERKLKTQEDLGNWEEWRDHSEEIRQHTLANLDYYLNMLSENVAAK
GGHVFFAETAEEANQYVQKVLRDKNAKKVAKSKSMVTEEIGLNEAMEAVGCEVIETDLGEYILQVDDHNPPSHIVVPALH
LNKEQIEKVFKEKLGYDKSSQPEELALFARQKLREEFLSADVGVTGCNFGVAESGSFSLVTNEGNARMVTTLPKTQITVM
GMERLVPTHEELEVLVSMLTRSAVGQKLTSYVTTLTGPRAEDEIDGPEEFHLIIVDNGRSKILGTQFQSILQCIRCAACV
NVCPVYRQTGGHSYGSIYPGPIGAVLSPLLGGYDTYKELPYASTLCGACTEACPVKIPLHDLLLEHRRVIVEEEHMSPVA
ERLVMKGFAQGASHSTLFKYGTKAAPALMSPFENKDKGMITKGPKPLQAWTNSRDFPMPDDENFRDWMQNRLKRGAK

Sequences:

>Translated_477_residues
MSMRIGNETFKQAVKTELKDDFMRGAVSSAQDRLRERKLKTQEDLGNWEEWRDHSEEIRQHTLANLDYYLNMLSENVAAK
GGHVFFAETAEEANQYVQKVLRDKNAKKVAKSKSMVTEEIGLNEAMEAVGCEVIETDLGEYILQVDDHNPPSHIVVPALH
LNKEQIEKVFKEKLGYDKSSQPEELALFARQKLREEFLSADVGVTGCNFGVAESGSFSLVTNEGNARMVTTLPKTQITVM
GMERLVPTHEELEVLVSMLTRSAVGQKLTSYVTTLTGPRAEDEIDGPEEFHLIIVDNGRSKILGTQFQSILQCIRCAACV
NVCPVYRQTGGHSYGSIYPGPIGAVLSPLLGGYDTYKELPYASTLCGACTEACPVKIPLHDLLLEHRRVIVEEEHMSPVA
ERLVMKGFAQGASHSTLFKYGTKAAPALMSPFENKDKGMITKGPKPLQAWTNSRDFPMPDDENFRDWMQNRLKRGAK
>Mature_476_residues
SMRIGNETFKQAVKTELKDDFMRGAVSSAQDRLRERKLKTQEDLGNWEEWRDHSEEIRQHTLANLDYYLNMLSENVAAKG
GHVFFAETAEEANQYVQKVLRDKNAKKVAKSKSMVTEEIGLNEAMEAVGCEVIETDLGEYILQVDDHNPPSHIVVPALHL
NKEQIEKVFKEKLGYDKSSQPEELALFARQKLREEFLSADVGVTGCNFGVAESGSFSLVTNEGNARMVTTLPKTQITVMG
MERLVPTHEELEVLVSMLTRSAVGQKLTSYVTTLTGPRAEDEIDGPEEFHLIIVDNGRSKILGTQFQSILQCIRCAACVN
VCPVYRQTGGHSYGSIYPGPIGAVLSPLLGGYDTYKELPYASTLCGACTEACPVKIPLHDLLLEHRRVIVEEEHMSPVAE
RLVMKGFAQGASHSTLFKYGTKAAPALMSPFENKDKGMITKGPKPLQAWTNSRDFPMPDDENFRDWMQNRLKRGAK

Specific function: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1786498, Length=469, Percent_Identity=55.2238805970149, Blast_Score=556, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LUTB_MACCJ (B9E9G9)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559576.1
- GeneID:   7390912
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0173
- OMA:   MAIADKG
- GO:   GO:0006810
- HAMAP:   MF_02103
- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825
- Gene3D:   G3DSA:3.40.50.10420
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR00273

Pfam domain/function: PF02589 DUF162; SSF46548 Helical_ferredxn

EC number: NA

Molecular weight: Translated: 53276; Mature: 53145

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMRIGNETFKQAVKTELKDDFMRGAVSSAQDRLRERKLKTQEDLGNWEEWRDHSEEIRQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
HTLANLDYYLNMLSENVAAKGGHVFFAETAEEANQYVQKVLRDKNAKKVAKSKSMVTEEI
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GLNEAMEAVGCEVIETDLGEYILQVDDHNPPSHIVVPALHLNKEQIEKVFKEKLGYDKSS
CHHHHHHHHCHHHHHHHHHHHEEEECCCCCCCCEEEEEHHCCHHHHHHHHHHHHCCCCCC
QPEELALFARQKLREEFLSADVGVTGCNFGVAESGSFSLVTNEGNARMVTTLPKTQITVM
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEE
GMERLVPTHEELEVLVSMLTRSAVGQKLTSYVTTLTGPRAEDEIDGPEEFHLIIVDNGRS
EHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
KILGTQFQSILQCIRCAACVNVCPVYRQTGGHSYGSIYPGPIGAVLSPLLGGYDTYKELP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCC
YASTLCGACTEACPVKIPLHDLLLEHRRVIVEEEHMSPVAERLVMKGFAQGASHSTLFKY
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHH
GTKAAPALMSPFENKDKGMITKGPKPLQAWTNSRDFPMPDDENFRDWMQNRLKRGAK
CCCCCHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SMRIGNETFKQAVKTELKDDFMRGAVSSAQDRLRERKLKTQEDLGNWEEWRDHSEEIRQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
HTLANLDYYLNMLSENVAAKGGHVFFAETAEEANQYVQKVLRDKNAKKVAKSKSMVTEEI
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GLNEAMEAVGCEVIETDLGEYILQVDDHNPPSHIVVPALHLNKEQIEKVFKEKLGYDKSS
CHHHHHHHHCHHHHHHHHHHHEEEECCCCCCCCEEEEEHHCCHHHHHHHHHHHHCCCCCC
QPEELALFARQKLREEFLSADVGVTGCNFGVAESGSFSLVTNEGNARMVTTLPKTQITVM
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEE
GMERLVPTHEELEVLVSMLTRSAVGQKLTSYVTTLTGPRAEDEIDGPEEFHLIIVDNGRS
EHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
KILGTQFQSILQCIRCAACVNVCPVYRQTGGHSYGSIYPGPIGAVLSPLLGGYDTYKELP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCC
YASTLCGACTEACPVKIPLHDLLLEHRRVIVEEEHMSPVAERLVMKGFAQGASHSTLFKY
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHH
GTKAAPALMSPFENKDKGMITKGPKPLQAWTNSRDFPMPDDENFRDWMQNRLKRGAK
CCCCCHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA