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The map label for this gene is lutR [H]

Identifier: 222150421

GI number: 222150421

Start: 177225

End: 177923

Strand: Direct

Name: lutR [H]

Synonym: MCCL_0171

Alternate gene names: 222150421

Gene position: 177225-177923 (Clockwise)

Preceding gene: 222150420

Following gene: 222150422

Centisome position: 8.43

GC content: 34.19

Gene sequence:

>699_bases
ATGGTGAAATTAGAACGAAAGAAACTATATGAAGAGATTGCTGAAATAATTACTGAGAAGATAAAGAACGGAGAATACAG
TAGGGGAGACAAATTGCCCTCAATTCAAGCTTTATCTAAAGAATACGGTGTGGGACAGGCCTCTATTCGTGAAGCGCTAA
ATGCATTACGTGTGATTGGTCTGCTGGAAATAAAGCATGGCGAGGGGACATTTGTTAAAGCGATTGAAAGCTCTGCATTT
ACACCTGAAATGGCACTATATGATAAGGAAGATATCAAGGATCTCCTTGAAGTGCGTAAAGTGCTTGAAGTCGGATCTGT
CAGAGCAGCTGCGATGCATAGATCTGAACAACAATTGGAAGCGATACACTATGCATTAAAACAAATGGAACAGGCAATGT
ATGACAAGGAAATAGGTGAGGAAAGTGATTTAAACTTTCATCTTTCTATTGCGGAAGCTTCTAATAACAAATTGTTAAAA
CATATGTTAATAGAAGTATCAGATAAAATTAAGACAGTGATGAAAGAAACAAGACAAATTTGGTTATTTAATGAAGAGAA
ATCTATTGAAAAACTTTTTAATGAACATACCGAAATTTTTCTGGCTATAAAAAATAGAAATATGAAACTTTCTGAGAAAA
AAATGCTTAAACACCTGAAAGACGTGGAAGAAGTATTAATAGAGCATTATAAAATATAA

Upstream 100 bases:

>100_bases
ATATATCATATCGAAAACAGGTGACGCATTGCGTTACCTGTTATTTTTATATTCTATAGTTTGGTATAATTAAGATATTA
AATTAGCGAGGGATATATAC

Downstream 100 bases:

>100_bases
TTTTGTCACTTGCTTTTAGAAAAACGTTGCAATAGAATAGAACTATTGGTCACCGAGTCATATGATGACTGTTTAATTTT
CAGCTGTGGGCACACTGAAA

Product: hypothetical protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MVKLERKKLYEEIAEIITEKIKNGEYSRGDKLPSIQALSKEYGVGQASIREALNALRVIGLLEIKHGEGTFVKAIESSAF
TPEMALYDKEDIKDLLEVRKVLEVGSVRAAAMHRSEQQLEAIHYALKQMEQAMYDKEIGEESDLNFHLSIAEASNNKLLK
HMLIEVSDKIKTVMKETRQIWLFNEEKSIEKLFNEHTEIFLAIKNRNMKLSEKKMLKHLKDVEEVLIEHYKI

Sequences:

>Translated_232_residues
MVKLERKKLYEEIAEIITEKIKNGEYSRGDKLPSIQALSKEYGVGQASIREALNALRVIGLLEIKHGEGTFVKAIESSAF
TPEMALYDKEDIKDLLEVRKVLEVGSVRAAAMHRSEQQLEAIHYALKQMEQAMYDKEIGEESDLNFHLSIAEASNNKLLK
HMLIEVSDKIKTVMKETRQIWLFNEEKSIEKLFNEHTEIFLAIKNRNMKLSEKKMLKHLKDVEEVLIEHYKI
>Mature_232_residues
MVKLERKKLYEEIAEIITEKIKNGEYSRGDKLPSIQALSKEYGVGQASIREALNALRVIGLLEIKHGEGTFVKAIESSAF
TPEMALYDKEDIKDLLEVRKVLEVGSVRAAAMHRSEQQLEAIHYALKQMEQAMYDKEIGEESDLNFHLSIAEASNNKLLK
HMLIEVSDKIKTVMKETRQIWLFNEEKSIEKLFNEHTEIFLAIKNRNMKLSEKKMLKHLKDVEEVLIEHYKI

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=235, Percent_Identity=23.8297872340426, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790032, Length=233, Percent_Identity=28.3261802575107, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789621, Length=226, Percent_Identity=26.5486725663717, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994955, Length=204, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789353, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786303, Length=231, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082208, Length=230, Percent_Identity=24.3478260869565, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26858; Mature: 26858

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLERKKLYEEIAEIITEKIKNGEYSRGDKLPSIQALSKEYGVGQASIREALNALRVIG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
LLEIKHGEGTFVKAIESSAFTPEMALYDKEDIKDLLEVRKVLEVGSVRAAAMHRSEQQLE
HHEEECCCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIHYALKQMEQAMYDKEIGEESDLNFHLSIAEASNNKLLKHMLIEVSDKIKTVMKETRQI
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHE
WLFNEEKSIEKLFNEHTEIFLAIKNRNMKLSEKKMLKHLKDVEEVLIEHYKI
EEECCHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKLERKKLYEEIAEIITEKIKNGEYSRGDKLPSIQALSKEYGVGQASIREALNALRVIG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
LLEIKHGEGTFVKAIESSAFTPEMALYDKEDIKDLLEVRKVLEVGSVRAAAMHRSEQQLE
HHEEECCCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIHYALKQMEQAMYDKEIGEESDLNFHLSIAEASNNKLLKHMLIEVSDKIKTVMKETRQI
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHE
WLFNEEKSIEKLFNEHTEIFLAIKNRNMKLSEKKMLKHLKDVEEVLIEHYKI
EEECCHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]