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The map label for this gene is lctD

Identifier: 222150413

GI number: 222150413

Start: 168291

End: 169238

Strand: Direct

Name: lctD

Synonym: MCCL_0163

Alternate gene names: 222150413

Gene position: 168291-169238 (Clockwise)

Preceding gene: 222150412

Following gene: 222150414

Centisome position: 8.0

GC content: 34.49

Gene sequence:

>948_bases
ATGGAAAAATTTAAAGGCAATAAAGTAGTTCTTGTAGGTAATGGTGCAGTAGGATCTAGTTATGCATTTGCGATGTTAAA
CCAAGGTGCTTGTGATGAATTTGTTATCATCGATCTTAATGAAGATAAAGCTAAAGGTGATGCAATGGATTTAAATCATG
GTGTCGTTTATGCACCGAGTCCAATGCAAGTTAAATATGGTACGTATGAAGATTGTCATGATGCATCTTTAATAGTTATT
TGTGCAGGAGCAGCACAAAAACCTGGAGAAACAAGACTAGATTTAGTTGGTAAGAATATGAAAATCTTTAAATCAATTGT
CGATGAGATTATGAAAAGTGGGTTTGATGGTATCTTCTTAATTGCAACGAACCCAGTAGATGTATTAACTTATGCTGTTC
AAAAATTCTCTGGATTACCTGAAAATCAAGTTATAGGATCTGGTACAATACTAGACACTGCGCGTTTTAGACATTTATTA
AGTCAAGAGTTTAATGTTTCTCCTAATAGTGTCCACGGTTATATTATTGGAGAGCATGGGGATTCGGAATTAGCGGTATG
GAGCGGTACAAATATTGCAGGAAATTCACTTTATGACATTTTGAATGAAAACCCTGAAAAACAAAAACTAATCGAAGAAA
TATTTGTTAATACGCGTGATGCGGCATACGAAATTATTAAAGCCAAAGGTGCCACTTATTACGGAGTTGCAATGGGCTTA
ATGCGTATTTCTAAAGCAATCTTAAATAATGAAAATGTTGTACTGACAGTATCTGCGAAATTAAATGGTGAGTATGGCCA
CGACGATGTATATATCGGCGTTCCTGCAATTATTAACAGAAATGGTATCAGAGAAGTATTAGAAACACCATTAAATACGG
AAGAAAAAGAAAAGTTTGCAAAATCGGTAGAAACTTTAAAAGCGATTCAAACACCATTTTTCAGCTAA

Upstream 100 bases:

>100_bases
GACATTGAAATATAGTATTGCATTACTTGTATTTGTTTGTATTTGGACATTCGCACTTAGTTTAATACTCTAATCAATTA
TTATTAAAGGAGAGATAAAG

Downstream 100 bases:

>100_bases
ATAAATTAAACGATAATTAATTGATTGCAATGGATAAACCCCCTGGACGGTATGAGGAAATTTCTCCTTCTATGACTGTC
TGGGGTTTTTATAAGTAGAG

Product: L-lactate dehydrogenase 1

Products: NA

Alternate protein names: L-LDH

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MEKFKGNKVVLVGNGAVGSSYAFAMLNQGACDEFVIIDLNEDKAKGDAMDLNHGVVYAPSPMQVKYGTYEDCHDASLIVI
CAGAAQKPGETRLDLVGKNMKIFKSIVDEIMKSGFDGIFLIATNPVDVLTYAVQKFSGLPENQVIGSGTILDTARFRHLL
SQEFNVSPNSVHGYIIGEHGDSELAVWSGTNIAGNSLYDILNENPEKQKLIEEIFVNTRDAAYEIIKAKGATYYGVAMGL
MRISKAILNNENVVLTVSAKLNGEYGHDDVYIGVPAIINRNGIREVLETPLNTEEKEKFAKSVETLKAIQTPFFS

Sequences:

>Translated_315_residues
MEKFKGNKVVLVGNGAVGSSYAFAMLNQGACDEFVIIDLNEDKAKGDAMDLNHGVVYAPSPMQVKYGTYEDCHDASLIVI
CAGAAQKPGETRLDLVGKNMKIFKSIVDEIMKSGFDGIFLIATNPVDVLTYAVQKFSGLPENQVIGSGTILDTARFRHLL
SQEFNVSPNSVHGYIIGEHGDSELAVWSGTNIAGNSLYDILNENPEKQKLIEEIFVNTRDAAYEIIKAKGATYYGVAMGL
MRISKAILNNENVVLTVSAKLNGEYGHDDVYIGVPAIINRNGIREVLETPLNTEEKEKFAKSVETLKAIQTPFFS
>Mature_315_residues
MEKFKGNKVVLVGNGAVGSSYAFAMLNQGACDEFVIIDLNEDKAKGDAMDLNHGVVYAPSPMQVKYGTYEDCHDASLIVI
CAGAAQKPGETRLDLVGKNMKIFKSIVDEIMKSGFDGIFLIATNPVDVLTYAVQKFSGLPENQVIGSGTILDTARFRHLL
SQEFNVSPNSVHGYIIGEHGDSELAVWSGTNIAGNSLYDILNENPEKQKLIEEIFVNTRDAAYEIIKAKGATYYGVAMGL
MRISKAILNNENVVLTVSAKLNGEYGHDDVYIGVPAIINRNGIREVLETPLNTEEKEKFAKSVETLKAIQTPFFS

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI5031857, Length=313, Percent_Identity=40.8945686900958, Blast_Score=252, Evalue=3e-67,
Organism=Homo sapiens, GI260099723, Length=313, Percent_Identity=40.8945686900958, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=41.2698412698413, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=41.2698412698413, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI291575128, Length=310, Percent_Identity=39.0322580645161, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI4557032, Length=310, Percent_Identity=39.0322580645161, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI15082234, Length=310, Percent_Identity=40.6451612903226, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI9257228, Length=307, Percent_Identity=40.7166123778502, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI4504973, Length=307, Percent_Identity=40.7166123778502, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=46.4285714285714, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=46.4285714285714, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI207028494, Length=210, Percent_Identity=40, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI103472011, Length=306, Percent_Identity=26.4705882352941, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI103472015, Length=183, Percent_Identity=28.4153005464481, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1789632, Length=294, Percent_Identity=23.469387755102, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=293, Percent_Identity=40.6143344709898, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17136226, Length=311, Percent_Identity=41.1575562700965, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI45550422, Length=318, Percent_Identity=31.1320754716981, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24647881, Length=251, Percent_Identity=26.6932270916335, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH_MACCJ (B9E9F9)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559566.1
- ProteinModelPortal:   B9E9F9
- GeneID:   7390905
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0163
- OMA:   MDLNHGV
- ProtClustDB:   CLSK2459958
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34367; Mature: 34367

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 179-179 BINDING 92-92 BINDING 124-124 BINDING 155-155 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFKGNKVVLVGNGAVGSSYAFAMLNQGACDEFVIIDLNEDKAKGDAMDLNHGVVYAPS
CCCCCCCEEEEEECCCCCCCHHEEEECCCCCCCEEEEECCCCCCCCCCEECCCCEEECCC
PMQVKYGTYEDCHDASLIVICAGAAQKPGETRLDLVGKNMKIFKSIVDEIMKSGFDGIFL
CCEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEE
IATNPVDVLTYAVQKFSGLPENQVIGSGTILDTARFRHLLSQEFNVSPNSVHGYIIGEHG
EECCCHHHHHHHHHHHCCCCCCCEECCCCEEHHHHHHHHHHHHCCCCCCCEEEEEEECCC
DSELAVWSGTNIAGNSLYDILNENPEKQKLIEEIFVNTRDAAYEIIKAKGATYYGVAMGL
CCCEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
MRISKAILNNENVVLTVSAKLNGEYGHDDVYIGVPAIINRNGIREVLETPLNTEEKEKFA
HHHHHHHHCCCCEEEEEEEEECCCCCCCEEEECCCCEECCCHHHHHHHCCCCCHHHHHHH
KSVETLKAIQTPFFS
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKFKGNKVVLVGNGAVGSSYAFAMLNQGACDEFVIIDLNEDKAKGDAMDLNHGVVYAPS
CCCCCCCEEEEEECCCCCCCHHEEEECCCCCCCEEEEECCCCCCCCCCEECCCCEEECCC
PMQVKYGTYEDCHDASLIVICAGAAQKPGETRLDLVGKNMKIFKSIVDEIMKSGFDGIFL
CCEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEE
IATNPVDVLTYAVQKFSGLPENQVIGSGTILDTARFRHLLSQEFNVSPNSVHGYIIGEHG
EECCCHHHHHHHHHHHCCCCCCCEECCCCEEHHHHHHHHHHHHCCCCCCCEEEEEEECCC
DSELAVWSGTNIAGNSLYDILNENPEKQKLIEEIFVNTRDAAYEIIKAKGATYYGVAMGL
CCCEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
MRISKAILNNENVVLTVSAKLNGEYGHDDVYIGVPAIINRNGIREVLETPLNTEEKEKFA
HHHHHHHHCCCCEEEEEEEEECCCCCCCEEEECCCCEECCCHHHHHHHCCCCCHHHHHHH
KSVETLKAIQTPFFS
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA