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The map label for this gene is ahpF [H]

Identifier: 222150349

GI number: 222150349

Start: 101662

End: 103185

Strand: Direct

Name: ahpF [H]

Synonym: MCCL_0099

Alternate gene names: 222150349

Gene position: 101662-103185 (Clockwise)

Preceding gene: 222150348

Following gene: 222150350

Centisome position: 4.84

GC content: 38.06

Gene sequence:

>1524_bases
ATGCTAGACAGTAGTTTAAAACAACAATTAAGTCAATACCTTGAGTTACTTGAAGGTGAGATTATTTTAACAGCAAGTAA
AGGTGAAGATAAAACTTCACAGGATATGATGGCATTATTAAATGAAATTAGTGAAATGTCGGACAAAATATCTATTAAAG
AAGCAGTGCTTAAGAGAACACCTAGCTTCACAGTTGCGCGTCCTGAAGAGGAGGCACGTGTTACTTTTGCAGGTGTACCA
TTAGGTCATGAATTTACATCATTAGTACTTGCTCTACTACAAGTGAGTGGCAGAGCTCCAAAACTTGATGAAGCGGTAAT
CAATCAAATTAAAAAGATTGATCAGCCTTTAAATTTTGAAACGTTTGTAAGTCTAACTTGTACGAAGTGTCCTGATGTTG
TCCAGGCATTGAACGTAATGTCGGTGCTTAATCCAAATATTACTCATACAATGATTGATGGTGCAGTATTCAGAGAAGAA
TCTAAAGATATTATGGCAGTACCTAGTATTTTCTTAGATGGTGAAGAGTTTGGCAGTGGCAAAATGACGGTTGAAGAAAT
CGTTAATCAAGTTGGTGGAGGCGCTGATGCATCAGAATTTGATGCAAGAGAGCCTTTCGATGTCCTTGTTATTGGTGGAG
GGCCTGCAAGTGCAGCAGCTTCAATATATGCTGCACGTAAAGGTTTAAGAACTGGTATTGTTGCAGAACGTATCGGTGGA
CAAGTAAATGATACTGCTGCAATTGAAAACTTTATTTCTGTAAAAGAAACAACAGGTCCGGAGTTTGCTCAGAACTTAGT
TGATCATATTAAAAGCTATGACATCGATGTGATGAATGCAGTGCGTGTAGAATCGATTGAGAAAATTGATGATATGAGTT
ATGTCACACTTGAGAACGGTGGAAAGCTTTCAAGTAAAACATTGATTATTAGTACAGGTGCAAAATGGCGTAATATGAAC
GTGCCAGGTGAAGCTGAATATAAAAATAAAGGTGTAGCTTATTGCCCGCATTGTGATGGTCCTTTATTTGAGGGTAAACG
TGTTGCAGTTGTTGGTGGCGGAAACTCTGGTGTAGAGGCTGCGATTGATTTAGCGGGTATTGTAAAGCATGTCACTTTAC
TTGAATTTGGTGAAGAATTAAGAGCTGATAAAGTATTACAGGACCGTCTTGCTTCGTTAGAAAATGCAGAGATAATTAAA
TCTGCAGCTACGAAAGAGTTGACAGGTACAGATAAACTTCAAGCCTTGACTTATGTAGATCGTACGACAAATGAAGAACA
TACAGTTGATTTAGAAGGTGTCTTCGTGCAAATTGGTTTAGTACCAAGTACTGAATTTGTGGGAGATTTAGTAGAGCGCA
ACAACCGTGGTGAAATTATCGTTGATCGTAATGGTGCTACGAATGTTCCAGGTATATTTGCAGCAGGAGATTGCACAGAT
CAAACATATAAACAAATTATTATTTCTATGGGTGCAGGTGCGACAGCAGCATTAAGCGCATTCGACTATTTAATTAGAAA
TTAA

Upstream 100 bases:

>100_bases
AACTTAGCGTTAATATCTATAGTGCCACAACGTATATATTGCGTTGTGGTCTCTATTATCTTGAGGGTGTATAAATATAC
AATAAAAGGAGCATAAGTAA

Downstream 100 bases:

>100_bases
GATTATCATCAATACCACTTAAGTAATAACTTACATTAAGTGGTATTTTTATTTTGTTTGTGCTTATAATAAAATGAGGA
GGAATATTATGAGAAAAATA

Product: alkyl hydroperoxide reductase (large subunit) and NADH dehydrogenase

Products: NA

Alternate protein names: Alkyl hydroperoxide reductase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLDSSLKQQLSQYLELLEGEIILTASKGEDKTSQDMMALLNEISEMSDKISIKEAVLKRTPSFTVARPEEEARVTFAGVP
LGHEFTSLVLALLQVSGRAPKLDEAVINQIKKIDQPLNFETFVSLTCTKCPDVVQALNVMSVLNPNITHTMIDGAVFREE
SKDIMAVPSIFLDGEEFGSGKMTVEEIVNQVGGGADASEFDAREPFDVLVIGGGPASAAASIYAARKGLRTGIVAERIGG
QVNDTAAIENFISVKETTGPEFAQNLVDHIKSYDIDVMNAVRVESIEKIDDMSYVTLENGGKLSSKTLIISTGAKWRNMN
VPGEAEYKNKGVAYCPHCDGPLFEGKRVAVVGGGNSGVEAAIDLAGIVKHVTLLEFGEELRADKVLQDRLASLENAEIIK
SAATKELTGTDKLQALTYVDRTTNEEHTVDLEGVFVQIGLVPSTEFVGDLVERNNRGEIIVDRNGATNVPGIFAAGDCTD
QTYKQIIISMGAGATAALSAFDYLIRN

Sequences:

>Translated_507_residues
MLDSSLKQQLSQYLELLEGEIILTASKGEDKTSQDMMALLNEISEMSDKISIKEAVLKRTPSFTVARPEEEARVTFAGVP
LGHEFTSLVLALLQVSGRAPKLDEAVINQIKKIDQPLNFETFVSLTCTKCPDVVQALNVMSVLNPNITHTMIDGAVFREE
SKDIMAVPSIFLDGEEFGSGKMTVEEIVNQVGGGADASEFDAREPFDVLVIGGGPASAAASIYAARKGLRTGIVAERIGG
QVNDTAAIENFISVKETTGPEFAQNLVDHIKSYDIDVMNAVRVESIEKIDDMSYVTLENGGKLSSKTLIISTGAKWRNMN
VPGEAEYKNKGVAYCPHCDGPLFEGKRVAVVGGGNSGVEAAIDLAGIVKHVTLLEFGEELRADKVLQDRLASLENAEIIK
SAATKELTGTDKLQALTYVDRTTNEEHTVDLEGVFVQIGLVPSTEFVGDLVERNNRGEIIVDRNGATNVPGIFAAGDCTD
QTYKQIIISMGAGATAALSAFDYLIRN
>Mature_507_residues
MLDSSLKQQLSQYLELLEGEIILTASKGEDKTSQDMMALLNEISEMSDKISIKEAVLKRTPSFTVARPEEEARVTFAGVP
LGHEFTSLVLALLQVSGRAPKLDEAVINQIKKIDQPLNFETFVSLTCTKCPDVVQALNVMSVLNPNITHTMIDGAVFREE
SKDIMAVPSIFLDGEEFGSGKMTVEEIVNQVGGGADASEFDAREPFDVLVIGGGPASAAASIYAARKGLRTGIVAERIGG
QVNDTAAIENFISVKETTGPEFAQNLVDHIKSYDIDVMNAVRVESIEKIDDMSYVTLENGGKLSSKTLIISTGAKWRNMN
VPGEAEYKNKGVAYCPHCDGPLFEGKRVAVVGGGNSGVEAAIDLAGIVKHVTLLEFGEELRADKVLQDRLASLENAEIIK
SAATKELTGTDKLQALTYVDRTTNEEHTVDLEGVFVQIGLVPSTEFVGDLVERNNRGEIIVDRNGATNVPGIFAAGDCTD
QTYKQIIISMGAGATAALSAFDYLIRN

Specific function: Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=520, Percent_Identity=52.6923076923077, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1787114, Length=310, Percent_Identity=34.5161290322581, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320560, Length=314, Percent_Identity=35.3503184713376, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321898, Length=314, Percent_Identity=32.484076433121, Blast_Score=148, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.6.99.3 [H]

Molecular weight: Translated: 54599; Mature: 54599

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSSLKQQLSQYLELLEGEIILTASKGEDKTSQDMMALLNEISEMSDKISIKEAVLKRT
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSFTVARPEEEARVTFAGVPLGHEFTSLVLALLQVSGRAPKLDEAVINQIKKIDQPLNFE
CCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHH
TFVSLTCTKCPDVVQALNVMSVLNPNITHTMIDGAVFREESKDIMAVPSIFLDGEEFGSG
HEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCEEECCHHCCCC
KMTVEEIVNQVGGGADASEFDAREPFDVLVIGGGPASAAASIYAARKGLRTGIVAERIGG
CEEHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHCC
QVNDTAAIENFISVKETTGPEFAQNLVDHIKSYDIDVMNAVRVESIEKIDDMSYVTLENG
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCC
GKLSSKTLIISTGAKWRNMNVPGEAEYKNKGVAYCPHCDGPLFEGKRVAVVGGGNSGVEA
CCCCCCEEEEECCCCEECCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCHHH
AIDLAGIVKHVTLLEFGEELRADKVLQDRLASLENAEIIKSAATKELTGTDKLQALTYVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
RTTNEEHTVDLEGVFVQIGLVPSTEFVGDLVERNNRGEIIVDRNGATNVPGIFAAGDCTD
CCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCH
QTYKQIIISMGAGATAALSAFDYLIRN
HHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDSSLKQQLSQYLELLEGEIILTASKGEDKTSQDMMALLNEISEMSDKISIKEAVLKRT
CCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSFTVARPEEEARVTFAGVPLGHEFTSLVLALLQVSGRAPKLDEAVINQIKKIDQPLNFE
CCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHH
TFVSLTCTKCPDVVQALNVMSVLNPNITHTMIDGAVFREESKDIMAVPSIFLDGEEFGSG
HEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCEEECCHHCCCC
KMTVEEIVNQVGGGADASEFDAREPFDVLVIGGGPASAAASIYAARKGLRTGIVAERIGG
CEEHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHCC
QVNDTAAIENFISVKETTGPEFAQNLVDHIKSYDIDVMNAVRVESIEKIDDMSYVTLENG
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCC
GKLSSKTLIISTGAKWRNMNVPGEAEYKNKGVAYCPHCDGPLFEGKRVAVVGGGNSGVEA
CCCCCCEEEEECCCCEECCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCHHH
AIDLAGIVKHVTLLEFGEELRADKVLQDRLASLENAEIIKSAATKELTGTDKLQALTYVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
RTTNEEHTVDLEGVFVQIGLVPSTEFVGDLVERNNRGEIIVDRNGATNVPGIFAAGDCTD
CCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCH
QTYKQIIISMGAGATAALSAFDYLIRN
HHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8125345; 8180695 [H]