Click here to switch to the map view.

The map label for this gene is walK [H]

Identifier: 222150272

GI number: 222150272

Start: 26887

End: 28725

Strand: Direct

Name: walK [H]

Synonym: MCCL_0022

Alternate gene names: 222150272

Gene position: 26887-28725 (Clockwise)

Preceding gene: 222150271

Following gene: 222150273

Centisome position: 1.28

GC content: 34.48

Gene sequence:

>1839_bases
ATGAAAGTGATCTTTAAAAAACTTCAGTCGTTACATATAAAGCTCGTTGTTATTTACGTATTATTAATTATTATTGGCAT
GCAAATTATCGGACTTTATTTTACAAATAATCTCGAAAAAGAGCTTACAAAGAATTTTAAAACAAATATTAATCAGCAAA
TAAAGTCTATCAACTATGATATTAGGACTGTATATAACGAAAATAAATCTTCACCAAATAAAATACAAAAAGAAGTACAG
AAAGTCGTAGATGAATATGCATTAAGAACAGAGATAGATACCATTCGTTTTATTGATGATTCAGAAGTTATCCTTGCGAC
AAGTAATGATACAACTAAAGAAAATATCAATCAGAAGATCAATGATAAACAGATACAAAATTCTTTATCGCTCGGGAAAA
CGAATGATCGTATTAAGCTGCGTGATGACCCTGGTGGTAAGGTAAGAGTTTGGGTAAAGAATTTTCCGATTAAAGATAAC
AATAAAATTCTTGGTGTAATTCATATTGAAGAGTCTATTGAGTCCGTATATGCACAGTTGATGAAAATCAATAGTATCTT
TATTATCGGTACGGGTCTGTCATTGTTAATTACAATTATTCTTGGGTTCTTTATCGCAAGAACTATTACTAAACCGATTG
CAGATATGCGTAATCAAGCACTAGAGATGTCTAAAGGGAACTACACAAATCGTGTTAAAGTATACGGTAACGATGAAATT
GGTGAGCTCGCACTAACTTTTAATAACTTATCTAAGCGTGTGCAAGAAGCGCAGGCGAATACAGAGAGTGAAAAGCGTAG
ACTCGATTCAGTAATCACGCACATGTCTGATGGGGTTATTGCGACAGATAGACGTGGCCGTGTCCGTATTGTTAATGAAA
TGGCACTTAAGATGCTTGATATGAATAAAGATGATGTAGAAGGACGTCATGTTCTGGATATTCTTGCAATCGAAGCGCAT
TATTCATTAGATGAACTGCAGGATATCGCAGGTGGTATTATCATTGATATTAATGAAAAAGAAAGGCTTATTGTAAGAGC
ATCGTTTAGTACCATTATTCAAGATACAGGGTTTATTACGGGTTATATCGCAGTACTGCATGATGTTACCGAACAACATC
ACGTTGAGAATGAACGACGTGAATTTGTTGCGAATGTATCACACGAATTACGTACGCCACTAACGAGTATGCGCAGTTAT
ATTGAAGCATTAGAAGAAGGCGCCTGGAAAGATGAGAATTTAGCGCCTCAATTTTTAAGTGTGACGAGAGAAGAAACAGA
TCGTATGATTCGTTTAGTCAATGATTTGCTTCAATTATCTAAGATGGATAATACAGATGATAATTTAAATAAAGAGTTGA
TTGATTTTAATATGTTTATTCACAAAATTATCAATCGCTTTGAGATGTCAGAAGGGAAGAATGCGACCTTTATACGTGAT
ATCCCTAAAAATGGTATATTCGTTGAAATCGATCCAGATAAGATGACACAAGTATTTGATAATGTAATTACGAATGCGTT
GAAGTACTCTAAAGGAAGAAAGAAAGTCGAATTTCACGTGAAACAAAATACCTTGTTCAATCGTATGACGATACAAATTA
AGGATAATGGTATCGGTATTCCTTTGAATAAAGTAGATAAAATATTTGATCGTTTCTTTCGTGTAGATAAAGCGCGAACA
CGTAAAATGGGCGGAACTGGGTTAGGTCTCGCAATTAGTAAGGAAATCATTGAAGCTCATAATGGGAGAATATGGGCTAA
TAGTAAGGAAGGGTACGGCACTTCGGTGTATATAACGTTACCTTGTGAAGTGCTAGATGATGGTGAATGGGATGTATAA

Upstream 100 bases:

>100_bases
AGACGTTTAAGAGAAAAGATTGAAGATGATGCAAGTCATCCAGAATATATTGTGACACGCCGTGGTGTAGGATATTTCTT
GCAGACGCAGGAGTAATAGA

Downstream 100 bases:

>100_bases
AAGTATCGTTAAAGGTCTTACACTTTTCACCCTTGTTTTGATGAGCGTTGTATTGACATATAAAATATGGAACTTCAAAC
CTAATCTTGTGAATATAGAG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MKVIFKKLQSLHIKLVVIYVLLIIIGMQIIGLYFTNNLEKELTKNFKTNINQQIKSINYDIRTVYNENKSSPNKIQKEVQ
KVVDEYALRTEIDTIRFIDDSEVILATSNDTTKENINQKINDKQIQNSLSLGKTNDRIKLRDDPGGKVRVWVKNFPIKDN
NKILGVIHIEESIESVYAQLMKINSIFIIGTGLSLLITIILGFFIARTITKPIADMRNQALEMSKGNYTNRVKVYGNDEI
GELALTFNNLSKRVQEAQANTESEKRRLDSVITHMSDGVIATDRRGRVRIVNEMALKMLDMNKDDVEGRHVLDILAIEAH
YSLDELQDIAGGIIIDINEKERLIVRASFSTIIQDTGFITGYIAVLHDVTEQHHVENERREFVANVSHELRTPLTSMRSY
IEALEEGAWKDENLAPQFLSVTREETDRMIRLVNDLLQLSKMDNTDDNLNKELIDFNMFIHKIINRFEMSEGKNATFIRD
IPKNGIFVEIDPDKMTQVFDNVITNALKYSKGRKKVEFHVKQNTLFNRMTIQIKDNGIGIPLNKVDKIFDRFFRVDKART
RKMGGTGLGLAISKEIIEAHNGRIWANSKEGYGTSVYITLPCEVLDDGEWDV

Sequences:

>Translated_612_residues
MKVIFKKLQSLHIKLVVIYVLLIIIGMQIIGLYFTNNLEKELTKNFKTNINQQIKSINYDIRTVYNENKSSPNKIQKEVQ
KVVDEYALRTEIDTIRFIDDSEVILATSNDTTKENINQKINDKQIQNSLSLGKTNDRIKLRDDPGGKVRVWVKNFPIKDN
NKILGVIHIEESIESVYAQLMKINSIFIIGTGLSLLITIILGFFIARTITKPIADMRNQALEMSKGNYTNRVKVYGNDEI
GELALTFNNLSKRVQEAQANTESEKRRLDSVITHMSDGVIATDRRGRVRIVNEMALKMLDMNKDDVEGRHVLDILAIEAH
YSLDELQDIAGGIIIDINEKERLIVRASFSTIIQDTGFITGYIAVLHDVTEQHHVENERREFVANVSHELRTPLTSMRSY
IEALEEGAWKDENLAPQFLSVTREETDRMIRLVNDLLQLSKMDNTDDNLNKELIDFNMFIHKIINRFEMSEGKNATFIRD
IPKNGIFVEIDPDKMTQVFDNVITNALKYSKGRKKVEFHVKQNTLFNRMTIQIKDNGIGIPLNKVDKIFDRFFRVDKART
RKMGGTGLGLAISKEIIEAHNGRIWANSKEGYGTSVYITLPCEVLDDGEWDV
>Mature_612_residues
MKVIFKKLQSLHIKLVVIYVLLIIIGMQIIGLYFTNNLEKELTKNFKTNINQQIKSINYDIRTVYNENKSSPNKIQKEVQ
KVVDEYALRTEIDTIRFIDDSEVILATSNDTTKENINQKINDKQIQNSLSLGKTNDRIKLRDDPGGKVRVWVKNFPIKDN
NKILGVIHIEESIESVYAQLMKINSIFIIGTGLSLLITIILGFFIARTITKPIADMRNQALEMSKGNYTNRVKVYGNDEI
GELALTFNNLSKRVQEAQANTESEKRRLDSVITHMSDGVIATDRRGRVRIVNEMALKMLDMNKDDVEGRHVLDILAIEAH
YSLDELQDIAGGIIIDINEKERLIVRASFSTIIQDTGFITGYIAVLHDVTEQHHVENERREFVANVSHELRTPLTSMRSY
IEALEEGAWKDENLAPQFLSVTREETDRMIRLVNDLLQLSKMDNTDDNLNKELIDFNMFIHKIINRFEMSEGKNATFIRD
IPKNGIFVEIDPDKMTQVFDNVITNALKYSKGRKKVEFHVKQNTLFNRMTIQIKDNGIGIPLNKVDKIFDRFFRVDKART
RKMGGTGLGLAISKEIIEAHNGRIWANSKEGYGTSVYITLPCEVLDDGEWDV

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG5002

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=340, Percent_Identity=30.2941176470588, Blast_Score=153, Evalue=4e-38,
Organism=Escherichia coli, GI1788549, Length=465, Percent_Identity=27.9569892473118, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788393, Length=228, Percent_Identity=34.2105263157895, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1786783, Length=225, Percent_Identity=31.5555555555556, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790346, Length=248, Percent_Identity=28.6290322580645, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788713, Length=233, Percent_Identity=27.0386266094421, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=31.5555555555556, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786912, Length=237, Percent_Identity=28.2700421940928, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=26.7441860465116, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1789808, Length=238, Percent_Identity=30.672268907563, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789149, Length=230, Percent_Identity=27.8260869565217, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787894, Length=223, Percent_Identity=25.1121076233184, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI48994928, Length=251, Percent_Identity=25.0996015936255, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790300, Length=246, Percent_Identity=29.6747967479675, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=233, Percent_Identity=23.175965665236, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790551, Length=247, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=247, Percent_Identity=24.6963562753036, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787374, Length=277, Percent_Identity=25.9927797833935, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790861, Length=206, Percent_Identity=23.7864077669903, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 70143; Mature: 70143

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50885 HAMP ; PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIFKKLQSLHIKLVVIYVLLIIIGMQIIGLYFTNNLEKELTKNFKTNINQQIKSINYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
IRTVYNENKSSPNKIQKEVQKVVDEYALRTEIDTIRFIDDSEVILATSNDTTKENINQKI
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHCC
NDKQIQNSLSLGKTNDRIKLRDDPGGKVRVWVKNFPIKDNNKILGVIHIEESIESVYAQL
CHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHHH
MKINSIFIIGTGLSLLITIILGFFIARTITKPIADMRNQALEMSKGNYTNRVKVYGNDEI
HHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GELALTFNNLSKRVQEAQANTESEKRRLDSVITHMSDGVIATDRRGRVRIVNEMALKMLD
CCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHC
MNKDDVEGRHVLDILAIEAHYSLDELQDIAGGIIIDINEKERLIVRASFSTIIQDTGFIT
CCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCEEEEECCCCEEEEEEHHHHHHHHCCHHH
GYIAVLHDVTEQHHVENERREFVANVSHELRTPLTSMRSYIEALEEGAWKDENLAPQFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
VTREETDRMIRLVNDLLQLSKMDNTDDNLNKELIDFNMFIHKIINRFEMSEGKNATFIRD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
IPKNGIFVEIDPDKMTQVFDNVITNALKYSKGRKKVEFHVKQNTLFNRMTIQIKDNGIGI
CCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEEECCCCCC
PLNKVDKIFDRFFRVDKARTRKMGGTGLGLAISKEIIEAHNGRIWANSKEGYGTSVYITL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEEE
PCEVLDDGEWDV
EEEEECCCCCCC
>Mature Secondary Structure
MKVIFKKLQSLHIKLVVIYVLLIIIGMQIIGLYFTNNLEKELTKNFKTNINQQIKSINYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
IRTVYNENKSSPNKIQKEVQKVVDEYALRTEIDTIRFIDDSEVILATSNDTTKENINQKI
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHCC
NDKQIQNSLSLGKTNDRIKLRDDPGGKVRVWVKNFPIKDNNKILGVIHIEESIESVYAQL
CHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHHH
MKINSIFIIGTGLSLLITIILGFFIARTITKPIADMRNQALEMSKGNYTNRVKVYGNDEI
HHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GELALTFNNLSKRVQEAQANTESEKRRLDSVITHMSDGVIATDRRGRVRIVNEMALKMLD
CCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHC
MNKDDVEGRHVLDILAIEAHYSLDELQDIAGGIIIDINEKERLIVRASFSTIIQDTGFIT
CCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCEEEEECCCCEEEEEEHHHHHHHHCCHHH
GYIAVLHDVTEQHHVENERREFVANVSHELRTPLTSMRSYIEALEEGAWKDENLAPQFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
VTREETDRMIRLVNDLLQLSKMDNTDDNLNKELIDFNMFIHKIINRFEMSEGKNATFIRD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
IPKNGIFVEIDPDKMTQVFDNVITNALKYSKGRKKVEFHVKQNTLFNRMTIQIKDNGIGI
CCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEEECCCCCC
PLNKVDKIFDRFFRVDKARTRKMGGTGLGLAISKEIIEAHNGRIWANSKEGYGTSVYITL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEEE
PCEVLDDGEWDV
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA