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The map label for this gene is mdeA [H]

Identifier: 222111240

GI number: 222111240

Start: 2192076

End: 2193389

Strand: Direct

Name: mdeA [H]

Synonym: Dtpsy_2048

Alternate gene names: 222111240

Gene position: 2192076-2193389 (Clockwise)

Preceding gene: 222111237

Following gene: 222111241

Centisome position: 57.74

GC content: 68.04

Gene sequence:

>1314_bases
ATGCCCGGATATTCCGACCCCGGCTTCGACACGCTGGCCCTGCACGCCGGCGCGCAGCCGGACCCCGCCACAGGTGCGCG
CGCCGTGCCGATCCACCTGAGCACCTCGTTCGTCTTCGAGTCGAGCGACCATGCGGCGGCCCTGTTCAACCTGGAGCGCC
CGGGCCATGTGTACAGCCGCCTGAGCAACCCCACCAACGCGGTGCTGGAGCAGCGCGTGGCAGCGCTGGAGGGCGGCGTG
GGGGCGATTGCCGTGGCCAGCGGGCAGGCCGCGCTGCACCTGGCGATTGCCACATTGATGGGCGCAGGCAGCCATATCGT
GGCCAGCACGGCGCTGTACGGCGGCAGCCAGAACCTGCTGCACTACACGCTGCGCCGCTTCGGCATTGACACCACCTTCG
TGAAGCCCGGCGACATCGACGGCTGGCGCGCCGCCATCCGCCCCAACACCAAGCTGTTCTTTGGCGAAACCGTAGGCAAC
CCCGGGCTGGAGGTGCTGGACATCCCCACGGTCGCGGACATTGCGCATGAAGCTGGCGTGCCCCTGCTGGTGGACTCCAC
CCTCACCTCGCCGTGGCTCATCAAGCCCTTCGAGCATGGCGCGGACCTGGTGTACCACTCGGCCACCAAGTTCCTCTCAG
GCCATGGCACGGTGATCGGCGGCGTCGTGGTCGATGGCGGCAGCTTCGACTGGGAAGGCTCCGGCCGCTTCCCCGAGTTG
ACCCAGCCCTACGACGGCTTTCACAACATGGTGTTCACCGAGGAGAGCACCGTGGGCGCCTTCCTGCTGCGCGCGCGCCG
CGAGGGCCTGCGCGACTTCGGCGCCTGCATGAGCCCGCACACCGCCTGGCTGATCCTGCAGGGCATTGAGACCCTGCCGC
TGCGCATGGAACGCCACATGCGCAATACCGAGAAGGTGGTGCAGTTCCTGGCCAGCCACCCGTTCGTCGAGCGCGTGGGC
CACCCGCTGCTCGAGTCCCACCACAGCCACCAGCTCGCGCAGCGCCTGCTGCCGCGCGGCGCGGGCTCGGTGTTCAGCTT
CGACCTCAAGGGCAACCGCGAGCAGGGCAAGACGTTCATCGAGACGCTCAAGCTCTTCAGCCACCTGGCCAATGTGGGTG
ACTGCCGCAGCTTGGTGATCCACCCCGCCTCCACCACGCACTTCCGCATGAGCGACGAAGCGCTCGCCGCGGGCGGCATC
AGCCAGGGCACGATCCGCCTGTCGATCGGACTGGAAGATGCCGACGACCTGATCGACGACCTCAAGCGGGCACTCAAGGC
TGCGGAGAAGCAAGCGGGAAAGGCAGGTGCGTGA

Upstream 100 bases:

>100_bases
ATGGGGAGTCTCCGAAGGGTTCTGCAGGCGCTGCATGCAGCCCGATAGCGAATAAATATAGCCCACAATGGCGCACAAAT
TCACCACTTTGGAGACATGA

Downstream 100 bases:

>100_bases
TGTACGTGCAAGTCAACAGCGCACAGACCTACTGCTACACCGGCGGCAAGCCCTTCGATGCCGCCAAGCCCACCGTGGTG
TTCATCCACGGCGTGCTCAA

Product: o-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MPGYSDPGFDTLALHAGAQPDPATGARAVPIHLSTSFVFESSDHAAALFNLERPGHVYSRLSNPTNAVLEQRVAALEGGV
GAIAVASGQAALHLAIATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIDTTFVKPGDIDGWRAAIRPNTKLFFGETVGN
PGLEVLDIPTVADIAHEAGVPLLVDSTLTSPWLIKPFEHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEGSGRFPEL
TQPYDGFHNMVFTEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMERHMRNTEKVVQFLASHPFVERVG
HPLLESHHSHQLAQRLLPRGAGSVFSFDLKGNREQGKTFIETLKLFSHLANVGDCRSLVIHPASTTHFRMSDEALAAGGI
SQGTIRLSIGLEDADDLIDDLKRALKAAEKQAGKAGA

Sequences:

>Translated_437_residues
MPGYSDPGFDTLALHAGAQPDPATGARAVPIHLSTSFVFESSDHAAALFNLERPGHVYSRLSNPTNAVLEQRVAALEGGV
GAIAVASGQAALHLAIATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIDTTFVKPGDIDGWRAAIRPNTKLFFGETVGN
PGLEVLDIPTVADIAHEAGVPLLVDSTLTSPWLIKPFEHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEGSGRFPEL
TQPYDGFHNMVFTEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMERHMRNTEKVVQFLASHPFVERVG
HPLLESHHSHQLAQRLLPRGAGSVFSFDLKGNREQGKTFIETLKLFSHLANVGDCRSLVIHPASTTHFRMSDEALAAGGI
SQGTIRLSIGLEDADDLIDDLKRALKAAEKQAGKAGA
>Mature_436_residues
PGYSDPGFDTLALHAGAQPDPATGARAVPIHLSTSFVFESSDHAAALFNLERPGHVYSRLSNPTNAVLEQRVAALEGGVG
AIAVASGQAALHLAIATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIDTTFVKPGDIDGWRAAIRPNTKLFFGETVGNP
GLEVLDIPTVADIAHEAGVPLLVDSTLTSPWLIKPFEHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEGSGRFPELT
QPYDGFHNMVFTEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMERHMRNTEKVVQFLASHPFVERVGH
PLLESHHSHQLAQRLLPRGAGSVFSFDLKGNREQGKTFIETLKLFSHLANVGDCRSLVIHPASTTHFRMSDEALAAGGIS
QGTIRLSIGLEDADDLIDDLKRALKAAEKQAGKAGA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=428, Percent_Identity=37.1495327102804, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI299473759, Length=428, Percent_Identity=34.3457943925234, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI25453487, Length=427, Percent_Identity=33.0210772833724, Blast_Score=158, Evalue=7e-39,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=35.2380952380952, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789383, Length=428, Percent_Identity=29.2056074766355, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=32.5301204819277, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=33.0985915492958, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=32.6291079812207, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=43.287037037037, Blast_Score=322, Evalue=6e-89,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=33.6448598130841, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=451, Percent_Identity=25.9423503325942, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=353, Percent_Identity=25.7790368271955, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=431, Percent_Identity=27.6102088167053, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=34.192037470726, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46837; Mature: 46705

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGYSDPGFDTLALHAGAQPDPATGARAVPIHLSTSFVFESSDHAAALFNLERPGHVYSR
CCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCHHHHH
LSNPTNAVLEQRVAALEGGVGAIAVASGQAALHLAIATLMGAGSHIVASTALYGGSQNLL
HCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
HYTLRRFGIDTTFVKPGDIDGWRAAIRPNTKLFFGETVGNPGLEVLDIPTVADIAHEAGV
HHHHHHHCCCEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCEEEECCHHHHHHHHCCC
PLLVDSTLTSPWLIKPFEHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEGSGRFPEL
CEEEECCCCCCEEECCHHCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCC
TQPYDGFHNMVFTEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMERHM
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
RNTEKVVQFLASHPFVERVGHPLLESHHSHQLAQRLLPRGAGSVFSFDLKGNREQGKTFI
CCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
ETLKLFSHLANVGDCRSLVIHPASTTHFRMSDEALAAGGISQGTIRLSIGLEDADDLIDD
HHHHHHHHHHCCCCCCEEEEECCCCCEEEECCHHHHCCCCCCCEEEEEECCCCHHHHHHH
LKRALKAAEKQAGKAGA
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PGYSDPGFDTLALHAGAQPDPATGARAVPIHLSTSFVFESSDHAAALFNLERPGHVYSR
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCHHHHH
LSNPTNAVLEQRVAALEGGVGAIAVASGQAALHLAIATLMGAGSHIVASTALYGGSQNLL
HCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
HYTLRRFGIDTTFVKPGDIDGWRAAIRPNTKLFFGETVGNPGLEVLDIPTVADIAHEAGV
HHHHHHHCCCEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCEEEECCHHHHHHHHCCC
PLLVDSTLTSPWLIKPFEHGADLVYHSATKFLSGHGTVIGGVVVDGGSFDWEGSGRFPEL
CEEEECCCCCCEEECCHHCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCC
TQPYDGFHNMVFTEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMERHM
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
RNTEKVVQFLASHPFVERVGHPLLESHHSHQLAQRLLPRGAGSVFSFDLKGNREQGKTFI
CCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
ETLKLFSHLANVGDCRSLVIHPASTTHFRMSDEALAAGGISQGTIRLSIGLEDADDLIDD
HHHHHHHHHHCCCCCCEEEEECCCCCEEEECCHHHHCCCCCCCEEEEEECCCCHHHHHHH
LKRALKAAEKQAGKAGA
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]