Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 222111168

GI number: 222111168

Start: 2112582

End: 2114096

Strand: Direct

Name: mltD [H]

Synonym: Dtpsy_1976

Alternate gene names: 222111168

Gene position: 2112582-2114096 (Clockwise)

Preceding gene: 222111167

Following gene: 222111174

Centisome position: 55.64

GC content: 64.69

Gene sequence:

>1515_bases
ATGAGACTTCTGCACATCCTGGGGCTGGCAAGCCTGCTGTGGTTGACCGGTTGCGCGACCACTGCCCCTACGGACAACGC
CGCATCCACCACCGACCCCACTACTGCCAGCCACACGCCGCTGTATCCGTCGGGGCCATTGCAGCCCATCACGGCGAGCG
CCGCCAGCGGCCACACAGTGGCTGGCGTCAGCGCGCCAGCCGATCTGTGGGACCGCATCCGGCGCGGCTTTTCCATGCCC
GACCTGGACACCGATCTGGTACGCGACCGGGAACAGTGGTATGCCGCGCGCCCCGACTACATGCAGCGCATGACCGAGCG
TTCCAGCAAGTACCTGTTCCATATCGTCGAAGAGCTGGAACGCCGGGGCATGCCCACAGAACTGGCGCTGCTGCCCTACA
TCGAGAGCGCCTTCAACCCTCAGGCAGTGTCCAGCGCCAAGGCCGCGGGCATGTGGCAGTTCATGCCGGCCACGGGAACC
TACTTCGACTTGAAGCAGAACGCCTTCCGCGACGACCGGCGCGATGTGCTGGCGTCCACGCGCGCGGCACTGGACTACCT
GCAGAAGCTCTACGGCATGTTCGGCGACTGGCATCTGGCCCTGGCCGCCTACAACTGGGGAGAAGGCAGCGTGGCCCGCG
CGATCGCGCGCAACGAGAAACAGGGTCTGGGCACGCGCTACACCGAGTTGACGATGCCCGCCGAAACCCGCATGTACGTT
CCCAAACTGCAGGCCGTGAAGAACATCGTGGCTAATCCCGACAAGTTCAGCACCGAGCTGCCGTTGATCGAGAACCATCC
GTACTTTCAGAGCGTGGAGATCACGCGCGACATGGACGTCACCCTGGTAGCCAGTCTCGCCGGCGTACGCCTCGAGGATT
TCCGCGCCCTGAACCCGTCGCTGCACAAGCCCGTGATCCTTGCGGCGGGCATGCCGCAGATCCTGCTGCCCTGGGAAAAC
GCCCAGGTGTTCCGCAAGAATCTGGAGGCCTACACCGAAGGCCAATACGCCAGTTGGACGGTATGGACGGTACCCAACAC
GATGAGCGTCTCCACCGCGGCGCAACGCACGGGCATGAGCGAAAACGATTTGCGTCAGGTGAATGGCATTCCACCACGCA
TGATGATCAAGGCCGGCTCGGCCCTCATGGTGCCCCGCGCAGCCACCATGCGCGCAGACGTCCCGGGTCATCTCGCCGAC
AACGGCCAAGTCAGCTTCACGCCTGAAATCGTGACGCGGCGCACCACGGTGCGCGCGGGCAAGCGCGACACCGTGGCCAG
CATCGCACGGCGCTACAAGGTCAGCACCGCAGACGTTGCCGACTGGAACGACGTGAAGACCTCCAGCAAGTTCAAGCCCG
GAGAACAGGTCGTGATGTACCTGCCCGTGCGGCTTGCCGCGACCTCGCTGGCGCGCACCAGCGCGAAACAACCCATCAGC
CGGGCGACAGTCCGCTCGGCAAAGGCCGGTGCGCCCACACGCAAGGGCGGCACACCGTCGAAACGCAAACGGTAA

Upstream 100 bases:

>100_bases
CTACGGTAGCCCAAGCCGCCCACGGCTTCGACGCCCTCATAGACCCGCGCGAACCCGCAGCCGTACTGGCCGCGCTGCGC
CAATGGAAAAACGAATTTCG

Downstream 100 bases:

>100_bases
GCCCGGCTAGAAACGCAGCGTGTGCATACCCAACAGGCCGAGGATGCCCATCACGATCAGGTACAGCGCAACGATCACGT
TGAGCAGTTTGGGCATGATC

Product: lytic transglycosylase subunit

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MRLLHILGLASLLWLTGCATTAPTDNAASTTDPTTASHTPLYPSGPLQPITASAASGHTVAGVSAPADLWDRIRRGFSMP
DLDTDLVRDREQWYAARPDYMQRMTERSSKYLFHIVEELERRGMPTELALLPYIESAFNPQAVSSAKAAGMWQFMPATGT
YFDLKQNAFRDDRRDVLASTRAALDYLQKLYGMFGDWHLALAAYNWGEGSVARAIARNEKQGLGTRYTELTMPAETRMYV
PKLQAVKNIVANPDKFSTELPLIENHPYFQSVEITRDMDVTLVASLAGVRLEDFRALNPSLHKPVILAAGMPQILLPWEN
AQVFRKNLEAYTEGQYASWTVWTVPNTMSVSTAAQRTGMSENDLRQVNGIPPRMMIKAGSALMVPRAATMRADVPGHLAD
NGQVSFTPEIVTRRTTVRAGKRDTVASIARRYKVSTADVADWNDVKTSSKFKPGEQVVMYLPVRLAATSLARTSAKQPIS
RATVRSAKAGAPTRKGGTPSKRKR

Sequences:

>Translated_504_residues
MRLLHILGLASLLWLTGCATTAPTDNAASTTDPTTASHTPLYPSGPLQPITASAASGHTVAGVSAPADLWDRIRRGFSMP
DLDTDLVRDREQWYAARPDYMQRMTERSSKYLFHIVEELERRGMPTELALLPYIESAFNPQAVSSAKAAGMWQFMPATGT
YFDLKQNAFRDDRRDVLASTRAALDYLQKLYGMFGDWHLALAAYNWGEGSVARAIARNEKQGLGTRYTELTMPAETRMYV
PKLQAVKNIVANPDKFSTELPLIENHPYFQSVEITRDMDVTLVASLAGVRLEDFRALNPSLHKPVILAAGMPQILLPWEN
AQVFRKNLEAYTEGQYASWTVWTVPNTMSVSTAAQRTGMSENDLRQVNGIPPRMMIKAGSALMVPRAATMRADVPGHLAD
NGQVSFTPEIVTRRTTVRAGKRDTVASIARRYKVSTADVADWNDVKTSSKFKPGEQVVMYLPVRLAATSLARTSAKQPIS
RATVRSAKAGAPTRKGGTPSKRKR
>Mature_504_residues
MRLLHILGLASLLWLTGCATTAPTDNAASTTDPTTASHTPLYPSGPLQPITASAASGHTVAGVSAPADLWDRIRRGFSMP
DLDTDLVRDREQWYAARPDYMQRMTERSSKYLFHIVEELERRGMPTELALLPYIESAFNPQAVSSAKAAGMWQFMPATGT
YFDLKQNAFRDDRRDVLASTRAALDYLQKLYGMFGDWHLALAAYNWGEGSVARAIARNEKQGLGTRYTELTMPAETRMYV
PKLQAVKNIVANPDKFSTELPLIENHPYFQSVEITRDMDVTLVASLAGVRLEDFRALNPSLHKPVILAAGMPQILLPWEN
AQVFRKNLEAYTEGQYASWTVWTVPNTMSVSTAAQRTGMSENDLRQVNGIPPRMMIKAGSALMVPRAATMRADVPGHLAD
NGQVSFTPEIVTRRTTVRAGKRDTVASIARRYKVSTADVADWNDVKTSSKFKPGEQVVMYLPVRLAATSLARTSAKQPIS
RATVRSAKAGAPTRKGGTPSKRKR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=467, Percent_Identity=31.0492505353319, Blast_Score=224, Evalue=9e-60,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 55678; Mature: 55678

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHILGLASLLWLTGCATTAPTDNAASTTDPTTASHTPLYPSGPLQPITASAASGHTV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
AGVSAPADLWDRIRRGFSMPDLDTDLVRDREQWYAARPDYMQRMTERSSKYLFHIVEELE
ECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RRGMPTELALLPYIESAFNPQAVSSAKAAGMWQFMPATGTYFDLKQNAFRDDRRDVLAST
HCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHH
RAALDYLQKLYGMFGDWHLALAAYNWGEGSVARAIARNEKQGLGTRYTELTMPAETRMYV
HHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHCCHH
PKLQAVKNIVANPDKFSTELPLIENHPYFQSVEITRDMDVTLVASLAGVRLEDFRALNPS
HHHHHHHHHHCCCCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHCCCCC
LHKPVILAAGMPQILLPWENAQVFRKNLEAYTEGQYASWTVWTVPNTMSVSTAAQRTGMS
CCCCEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCC
ENDLRQVNGIPPRMMIKAGSALMVPRAATMRADVPGHLADNGQVSFTPEIVTRRTTVRAG
HHHHHHHCCCCCHHEEECCCEEECCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHCCC
KRDTVASIARRYKVSTADVADWNDVKTSSKFKPGEQVVMYLPVRLAATSLARTSAKQPIS
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCHH
RATVRSAKAGAPTRKGGTPSKRKR
HHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLLHILGLASLLWLTGCATTAPTDNAASTTDPTTASHTPLYPSGPLQPITASAASGHTV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
AGVSAPADLWDRIRRGFSMPDLDTDLVRDREQWYAARPDYMQRMTERSSKYLFHIVEELE
ECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RRGMPTELALLPYIESAFNPQAVSSAKAAGMWQFMPATGTYFDLKQNAFRDDRRDVLAST
HCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHH
RAALDYLQKLYGMFGDWHLALAAYNWGEGSVARAIARNEKQGLGTRYTELTMPAETRMYV
HHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHCCHH
PKLQAVKNIVANPDKFSTELPLIENHPYFQSVEITRDMDVTLVASLAGVRLEDFRALNPS
HHHHHHHHHHCCCCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHCCCCC
LHKPVILAAGMPQILLPWENAQVFRKNLEAYTEGQYASWTVWTVPNTMSVSTAAQRTGMS
CCCCEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCC
ENDLRQVNGIPPRMMIKAGSALMVPRAATMRADVPGHLADNGQVSFTPEIVTRRTTVRAG
HHHHHHHCCCCCHHEEECCCEEECCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHCCC
KRDTVASIARRYKVSTADVADWNDVKTSSKFKPGEQVVMYLPVRLAATSLARTSAKQPIS
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCHH
RATVRSAKAGAPTRKGGTPSKRKR
HHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]