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The map label for this gene is gloB [H]

Identifier: 222111167

GI number: 222111167

Start: 2111806

End: 2112585

Strand: Direct

Name: gloB [H]

Synonym: Dtpsy_1975

Alternate gene names: 222111167

Gene position: 2111806-2112585 (Clockwise)

Preceding gene: 222111164

Following gene: 222111168

Centisome position: 55.62

GC content: 63.97

Gene sequence:

>780_bases
ATGAAGAACTTGCTGCCATTGCCCGCCTTCGCCGACAACTACATCTGGATGTTGTACGACGCGCAGCGTGCGGTCGTTGT
GGATCCCGGCGATGCAGAACCCGTGTTGCAGGCGCTGCGCAGCATGGGGCTGCCATTGGAAGCGATTCTAGTCACGCACC
ACCATGGCGACCACGTGGGCGGGGTGAACGCCCTGCGCGAAGCCACGGGCGCAACCGTGTATGGCCCTGCCACGGAGCCC
ATGCCCGAGCCCCTCATCCGCCTGTCGGGCGGCGACACTCTTCAGGTGCTAGGCCTCACGTTTTCCGTGCTGGACGTGCC
GGGGCATACGGCGGGGCATATCGCCTACTACTGTGCGGACATGGAAGGCGAGCCCCTGCTGTTGTGCGGGGATACGCTGT
TCTCAGGTGGATGCGGGCGCTTGTTTGAAGGCACGCCCACGCAAATGCAGGCCTCGCTGGATACGCTGGCTGCATTGCCC
GGCAACACCCGCGTATGCTGCGCCCATGAATACACGCTGTCCAACCTGAAGTTCGCCCGCGCGGTGGAACCCCATAACGC
TGCGCTACTCCAGTACATCACCGAATGCGAGTCCCGGCGCCGCGCGGATCAGCCCACACTGCCGTCGCGCATTGCCATCG
AGCGCGACATCAACCCTTTTCTGCGCACGCGCGTTGCTACGGTAGCCCAAGCCGCCCACGGCTTCGACGCCCTCATAGAC
CCGCGCGAACCCGCAGCCGTACTGGCCGCGCTGCGCCAATGGAAAAACGAATTTCGATGA

Upstream 100 bases:

>100_bases
GTCCGCCACCAGCTCGTCGTAACGCTCCTGCTCCCAGGCCAGCAGGTAGCGGCCAGGGGCAGTATCGAACCAGTGGTGCA
AACCTATAATTTCGCCGGTC

Downstream 100 bases:

>100_bases
GACTTCTGCACATCCTGGGGCTGGCAAGCCTGCTGTGGTTGACCGGTTGCGCGACCACTGCCCCTACGGACAACGCCGCA
TCCACCACCGACCCCACTAC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKNLLPLPAFADNYIWMLYDAQRAVVVDPGDAEPVLQALRSMGLPLEAILVTHHHGDHVGGVNALREATGATVYGPATEP
MPEPLIRLSGGDTLQVLGLTFSVLDVPGHTAGHIAYYCADMEGEPLLLCGDTLFSGGCGRLFEGTPTQMQASLDTLAALP
GNTRVCCAHEYTLSNLKFARAVEPHNAALLQYITECESRRRADQPTLPSRIAIERDINPFLRTRVATVAQAAHGFDALID
PREPAAVLAALRQWKNEFR

Sequences:

>Translated_259_residues
MKNLLPLPAFADNYIWMLYDAQRAVVVDPGDAEPVLQALRSMGLPLEAILVTHHHGDHVGGVNALREATGATVYGPATEP
MPEPLIRLSGGDTLQVLGLTFSVLDVPGHTAGHIAYYCADMEGEPLLLCGDTLFSGGCGRLFEGTPTQMQASLDTLAALP
GNTRVCCAHEYTLSNLKFARAVEPHNAALLQYITECESRRRADQPTLPSRIAIERDINPFLRTRVATVAQAAHGFDALID
PREPAAVLAALRQWKNEFR
>Mature_259_residues
MKNLLPLPAFADNYIWMLYDAQRAVVVDPGDAEPVLQALRSMGLPLEAILVTHHHGDHVGGVNALREATGATVYGPATEP
MPEPLIRLSGGDTLQVLGLTFSVLDVPGHTAGHIAYYCADMEGEPLLLCGDTLFSGGCGRLFEGTPTQMQASLDTLAALP
GNTRVCCAHEYTLSNLKFARAVEPHNAALLQYITECESRRRADQPTLPSRIAIERDINPFLRTRVATVAQAAHGFDALID
PREPAAVLAALRQWKNEFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=259, Percent_Identity=39.7683397683398, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI94538322, Length=259, Percent_Identity=39.7683397683398, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI21703352, Length=237, Percent_Identity=39.2405063291139, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI116642887, Length=237, Percent_Identity=39.2405063291139, Blast_Score=147, Evalue=6e-36,
Organism=Homo sapiens, GI14150041, Length=222, Percent_Identity=39.1891891891892, Blast_Score=140, Evalue=9e-34,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=37.8787878787879, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786406, Length=258, Percent_Identity=48.8372093023256, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1787158, Length=205, Percent_Identity=32.1951219512195, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536925, Length=257, Percent_Identity=34.2412451361868, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320478, Length=270, Percent_Identity=31.8518518518519, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324614, Length=269, Percent_Identity=28.996282527881, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=40.3846153846154, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=40.3846153846154, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=40.3846153846154, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI221330176, Length=169, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28090; Mature: 28090

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00743 BETA_LACTAMASE_B_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLPLPAFADNYIWMLYDAQRAVVVDPGDAEPVLQALRSMGLPLEAILVTHHHGDHVG
CCCCCCCCCCCCCEEEEEEECCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCC
GVNALREATGATVYGPATEPMPEPLIRLSGGDTLQVLGLTFSVLDVPGHTAGHIAYYCAD
HHHHHHHHCCCEEECCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCCCCCCEEEEEEEC
MEGEPLLLCGDTLFSGGCGRLFEGTPTQMQASLDTLAALPGNTRVCCAHEYTLSNLKFAR
CCCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCHHH
AVEPHNAALLQYITECESRRRADQPTLPSRIAIERDINPFLRTRVATVAQAAHGFDALID
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHCC
PREPAAVLAALRQWKNEFR
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNLLPLPAFADNYIWMLYDAQRAVVVDPGDAEPVLQALRSMGLPLEAILVTHHHGDHVG
CCCCCCCCCCCCCEEEEEEECCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCC
GVNALREATGATVYGPATEPMPEPLIRLSGGDTLQVLGLTFSVLDVPGHTAGHIAYYCAD
HHHHHHHHCCCEEECCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCCCCCCEEEEEEEC
MEGEPLLLCGDTLFSGGCGRLFEGTPTQMQASLDTLAALPGNTRVCCAHEYTLSNLKFAR
CCCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCHHH
AVEPHNAALLQYITECESRRRADQPTLPSRIAIERDINPFLRTRVATVAQAAHGFDALID
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHCC
PREPAAVLAALRQWKNEFR
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA