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The map label for this gene is yagI [H]

Identifier: 222111138

GI number: 222111138

Start: 2082152

End: 2082964

Strand: Direct

Name: yagI [H]

Synonym: Dtpsy_1946

Alternate gene names: 222111138

Gene position: 2082152-2082964 (Clockwise)

Preceding gene: 222111137

Following gene: 222111146

Centisome position: 54.84

GC content: 65.56

Gene sequence:

>813_bases
ATGAAGAAACACTCGCCGGACGCGCCCGCCAAACCCAGTGTCCAGGTGCTGGAGCGCATGTTCCAGTTGATTGACGTACT
CGCTTCGCGCGAGGAAGCCATCTCGCTGAAGGAGATCAGCGAGAAGACGGGCCTGCATCCGTCCACCACCCACCGCATCC
TCAATGACCTGACCATCGGCCGTTTCGTGGACCGTCCTGAGTCGGGCAGCTACCGCCTGGGCATGCGCCTGCTGGAGCTG
GGCAATCTGGTGAAGGGGCGCCTGAGCGTGCGCGACGCCGCGATGGCACCCATGCGGCAATTGCACAAGCAGATCCAGCA
ACCGGTGAACCTCAGCGTGCGCCAGGGGGACGAGATCGTGTACGTGGAACGCGCCTACAGCGAGCGTTCGGGCATGCAGG
TGGTGCGCGCCATTGGCGGCCACGCCCCGCTGCACCTCACCTCTACGGGCAAGCTCTTCCTTGCGGCCGACGATCCGCAA
CGCGTGCGCGCGTACGCCACGCGTACCGGCCTGCCAGGGCACACGCGCAACAGCATCACCCAGTTGCCCATCCTGGAACG
TGAACTGGGCAAGGCGCGCCAATACGGCATTGCGCGGGACAACGAGGAGTTGGAACTCGGGGTGCGCTGCATCGCGGCGG
GGGTCTTTGACGACCAGGGCCAGCTCGTGGCAGGCCTGTCAATTTCAGCGCCCGCCGATCGACTGGATGACGGATGGCTG
CCCAAGCTCCAGTCCACCGCCGATGAAATCTCCCAGGCGCTGGGTTATACGGCGGGGCAGCGCACGGCCCTGGACGGCCC
GCTCAAGACCTGA

Upstream 100 bases:

>100_bases
CGCACTGAGGAAGGCACGGACCGGCCGGACAGCCAGTGCGTAGAAGCAGCGCGGCATTTCACAAGATAAAATCACCGGTT
CCTTGGTTCTGCCCTGCCTC

Downstream 100 bases:

>100_bases
ACGGCCTGACGCCGGCCGTCCGGCGGGTGGGTCGCAAGACGCATGCGACCCGTGGCCGCAACCGCGGCTAACCGTTGGTT
CCTGCGTGCAGGATGGCGCC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKKHSPDAPAKPSVQVLERMFQLIDVLASREEAISLKEISEKTGLHPSTTHRILNDLTIGRFVDRPESGSYRLGMRLLEL
GNLVKGRLSVRDAAMAPMRQLHKQIQQPVNLSVRQGDEIVYVERAYSERSGMQVVRAIGGHAPLHLTSTGKLFLAADDPQ
RVRAYATRTGLPGHTRNSITQLPILERELGKARQYGIARDNEELELGVRCIAAGVFDDQGQLVAGLSISAPADRLDDGWL
PKLQSTADEISQALGYTAGQRTALDGPLKT

Sequences:

>Translated_270_residues
MKKHSPDAPAKPSVQVLERMFQLIDVLASREEAISLKEISEKTGLHPSTTHRILNDLTIGRFVDRPESGSYRLGMRLLEL
GNLVKGRLSVRDAAMAPMRQLHKQIQQPVNLSVRQGDEIVYVERAYSERSGMQVVRAIGGHAPLHLTSTGKLFLAADDPQ
RVRAYATRTGLPGHTRNSITQLPILERELGKARQYGIARDNEELELGVRCIAAGVFDDQGQLVAGLSISAPADRLDDGWL
PKLQSTADEISQALGYTAGQRTALDGPLKT
>Mature_270_residues
MKKHSPDAPAKPSVQVLERMFQLIDVLASREEAISLKEISEKTGLHPSTTHRILNDLTIGRFVDRPESGSYRLGMRLLEL
GNLVKGRLSVRDAAMAPMRQLHKQIQQPVNLSVRQGDEIVYVERAYSERSGMQVVRAIGGHAPLHLTSTGKLFLAADDPQ
RVRAYATRTGLPGHTRNSITQLPILERELGKARQYGIARDNEELELGVRCIAAGVFDDQGQLVAGLSISAPADRLDDGWL
PKLQSTADEISQALGYTAGQRTALDGPLKT

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=257, Percent_Identity=32.6848249027237, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789999, Length=251, Percent_Identity=34.6613545816733, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788131, Length=258, Percent_Identity=31.0077519379845, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786715, Length=254, Percent_Identity=30.3149606299213, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082359, Length=233, Percent_Identity=30.4721030042918, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790752, Length=227, Percent_Identity=27.3127753303965, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788581, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29645; Mature: 29645

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHSPDAPAKPSVQVLERMFQLIDVLASREEAISLKEISEKTGLHPSTTHRILNDLTIG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RFVDRPESGSYRLGMRLLELGNLVKGRLSVRDAAMAPMRQLHKQIQQPVNLSVRQGDEIV
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEE
YVERAYSERSGMQVVRAIGGHAPLHLTSTGKLFLAADDPQRVRAYATRTGLPGHTRNSIT
EEEHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCHH
QLPILERELGKARQYGIARDNEELELGVRCIAAGVFDDQGQLVAGLSISAPADRLDDGWL
HCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCC
PKLQSTADEISQALGYTAGQRTALDGPLKT
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKHSPDAPAKPSVQVLERMFQLIDVLASREEAISLKEISEKTGLHPSTTHRILNDLTIG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RFVDRPESGSYRLGMRLLELGNLVKGRLSVRDAAMAPMRQLHKQIQQPVNLSVRQGDEIV
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEE
YVERAYSERSGMQVVRAIGGHAPLHLTSTGKLFLAADDPQRVRAYATRTGLPGHTRNSIT
EEEHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCHH
QLPILERELGKARQYGIARDNEELELGVRCIAAGVFDDQGQLVAGLSISAPADRLDDGWL
HCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCC
PKLQSTADEISQALGYTAGQRTALDGPLKT
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]